Article
Virology
Jianqiu Qin, Tengyue Yan, Liujiang Yin, Cheng Yang, Liang Wang, Hong Qiu, Yanling Hu, Bin Xu
Summary: This study investigated the genotype diversity and epidemiology of human adenoviruses (HAdV) associated with respiratory tract infections (RTIs) in Nanning. The results showed significant variations in the age and gender distribution of HAdV-positive patients, with the highest positivity rate in the 0-6 age group. HAdV detection peaked during spring and autumn, especially in February. Genotyping identified the co-circulation of HAdV-B, HAdV-C, and HAdV-E in Nanning, with HAdV-C1, HAdV-C6, and HAdV-E4 being the most commonly detected genotypes.
Article
Microbiology
Xiaowei Wu, Jing Zhang, Wendong Lan, Lulu Quan, Junxian Ou, Wei Zhao, Jianguo Wu, Patrick C. Y. Woo, Donald Seto, Qiwei Zhang
Summary: This study presents a rapid, simple, and cost-effective method for molecular typing HAdV respiratory pathogens. It allows for the identification of HAdV genotypes and recombinants without the need for whole genome sequencing, making it suitable for real-time surveillance of circulating adenovirus strains in outbreaks and populations.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Immunology
Coraline Cheneau, Karsten Eichholz, Tuan Hiep Tran, Thi Thu Phuong Tran, Oceane Paris, Corinne Henriquet, Jeffrey J. Bajramovic, Martine Pugniere, Eric J. Kremer
Summary: Despite limited understanding of our innate immune response to human adenoviruses (HAdVs) and their vectors, this study found that lactoferrin can redirect HAdV types and induce an NLRP3-associated response in human phagocytes, impacting the immunogenicity of HAdVs and potentially enhancing the efficacy of HAdV-based vectors/vaccines.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Microbiology
Tailong Wang, Fanliang Meng, Changxiu Chen, Yesheng Shen, Peixun Li, Jie Xu, Zhaoyang Feng, Xiuchao Qu, Fuyong Wang, Baoquan Li, Mengda Liu
Summary: In recent years, the poultry industry in China has been greatly affected by adenoviral diseases caused by fowl adenovirus (FAdV). Shandong Province is an important area for poultry breeding and is seeing the emergence of various FAdV serotypes. A survey was conducted to determine the pathogenicity and epidemiology of FAdV, revealing that the dominant serotypes in the region were FAdV-2, FAdV-4, FAdV-8b, and FAdV-11. These serotypes caused high mortality rates and specific clinical signs in infected chicks. The findings contribute to the understanding of FAdV in poultry flocks and can aid in the development of vaccines and epidemic prevention strategies.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Selas T. F. Bots, Vera Kemp, Iris J. C. Dautzenberg, Rob C. Hoeben
Summary: The study analyzed the sequence of ten new adenoviruses (AdVs) isolated from healthy gorillas, bonobos, chimpanzees, and orangutans. The analysis showed that these ape-derived viruses are markedly distinct from human AdVs in terms of genome size, nucleotide composition, AdV protein sequence, and penton-base protein structure. The study also suggests that these viruses should be given species-level ranks and found evidence of recombination between orangutan and gorilla AdVs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Medicine, General & Internal
Dharmendra Shah, Shivani R. Chaudhary, Shahin Khan, Shashwat Mallik
Summary: We present a case of a giant retroperitoneal ganglioneuroma in a five-year-old child, which mimicked a mesenteric cyst on imaging and posed challenges in its management. This case serves as a reminder for clinicians to obtain histopathological confirmation before surgery.
CUREUS JOURNAL OF MEDICAL SCIENCE
(2023)
Article
Immunology
Yaru Li, Xia Yang, Renshuang Zhao, Zhiru Xiu, Shanzhi Li, Yue Li, Gaojie Song, Chenchen Ge, Jinbo Fang, Jicheng Han, Yilong Zhu, Yiquan Li
Summary: This study designed a recombinant subunit vaccine expressing adenovirus type 7 hexon protein using the baculovirus-insect cell expression system. The vaccine promoted antigen presenting cell maturation, improved antigen uptake capability, and triggered strong neutralizing antibody and cellular immune responses.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Microbiology
Renuka R. Nayak, Margaret Alexander, Ishani Deshpande, Kye Stapleton-Gray, Bipin Rimal, Andrew D. Patterson, Carles Ubeda, Jose U. Scher, Peter J. Turnbaugh
Summary: Immunomodulatory drug MTX can broadly alter human gut microbiota, especially in RA patients, with distinct effects on bacterial taxa and gene abundance between responders and non-responders. Transplantation of post-treatment samples into germ-free mice shows reduced immune activation, identifying MTX-modulated bacterial taxa associated with intestinal and splenic immune cells.
CELL HOST & MICROBE
(2021)
Article
Genetics & Heredity
Poonnada Jiraanont, Esther Manor, Nazi Tabatadze, Marwa Zafarullah, Guadalupe Mendoza, Gia Melikishvili, Flora Tassone
Summary: Fragile X syndrome is the most common cause of X-linked inherited intellectual disabilities and the most frequent monogenic form of autism spectrum disorders. It is caused by CGG trinucleotide repeat expansion in the FMR1 gene, leading to the absence of the fragile X mental retardation protein, FMRP. Other mechanisms such as deletions or point mutations of the FMR1 gene can also cause fragile X syndrome.
FRONTIERS IN GENETICS
(2022)
Article
Microbiology
Shan Zhao, Wenyi Guan, Kui Ma, Yuqian Yan, Junxian Ou, Jing Zhang, Zhiwu Yu, Jianguo Wu, Qiwei Zhang
Summary: Whole-genome sequencing plays a crucial role in identifying, typing, and mutation analysis of human adenoviruses (HAdVs). The first-generation sequencing method developed in this study allows for accurate and quick sequencing of common HAdV species B, C, and E. This novel method has been successfully applied to sequence the complete genomes of HAdV strains isolated from children with acute respiratory diseases, demonstrating high accuracy and efficiency.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Gastroenterology & Hepatology
Brian P. Lee, Giuseppe Cullaro, Aidan Vosooghi, Frederick Yao, Sarjukumar Panchal, David S. Goldberg, Norah A. Terrault, Nadim Mahmud
Summary: In this study, we found that the categorization of ACLF by UNOS showed weak agreement with manual chart review. These findings are informative for ongoing allocation policy discussions, highlight the importance of prospective studies regarding ACLF in liver transplantation, and should encourage UNOS reform.
JOURNAL OF HEPATOLOGY
(2022)
Article
Pharmacology & Pharmacy
Yiquan Li, Zhiru Xiu, Shanzhi Li, Yilong Zhu, Yaru Li, Renshuang Zhao, Yue Li, Xia Yang, Chenchen Ge, Nan Li, Ningyi Jin, Chao Shang, Xiao Li, Jicheng Han
Summary: Human adenovirus type 7 (HAdv-7) is the main cause of respiratory tract infections in children, but there are no available drugs or vaccines. In this study, a virus-like particle vaccine expressing the epitopes of HAdv-7 was developed and shown to induce strong immune responses. The vaccine activated the innate immune response and triggered neutralizing antibodies and cellular immune response.
ANTIVIRAL RESEARCH
(2023)
Article
Oncology
Emily A. Bates, James A. Davies, Jana Vanova, Davor Nestic, Valerie S. Meniel, Sarah Koushyar, Tabitha G. Cunliffe, Rosie M. Mundy, Elise Moses, Hanni K. Uusi-Kerttula, Alexander T. Baker, David K. Cole, Dragomira Majhen, Pierre J. Rizkallah, Toby Phesse, John D. Chester, Alan L. Parker
Summary: Oncolytic virotherapies based on human adenoviruses show great clinical potential. Incorporating a peptide that selectively targets alpha v beta 6 integrin, the HAdV-D10 serotype vector can effectively target alpha v beta 6-positive tumor cells. Structural and biological studies show that HAdV-D10 has weak interactions with adenoviral receptors and does not engage blood coagulation factor X, reducing off-target hepatice sequestration. In vitro and in vivo experiments demonstrate that HAdV-D10.A20 selectively kills cancer cells and has significant potential for clinical translation.
MOLECULAR THERAPY-ONCOLYTICS
(2022)
Article
Biotechnology & Applied Microbiology
Aleksi J. Leikas, Nihay Laham-Karam, Eline Agtereek, Hanna M. Peltonen, Tuomas Selander, Petra Korpisalo, Lari Holappa, Juha E. K. Hartikainen, Tommi Heikura, Seppo Yla-Herttuala
Summary: This study compared three different levels of replication-competent adenoviruses (RCA) in VEGF-D gene therapy production lots in a preclinical rabbit model and found no significant differences in efficacy or safety between the groups. Importantly, no detectable presence of RCA-specific E1 sequence was found in any samples tested, indicating that no detectable vector replication occurred in vivo. This suggests that relatively low levels of RCA in adenoviral gene therapy products may not pose a major safety concern as previously thought.
HUMAN GENE THERAPY
(2021)
Review
Cell Biology
Michele Palamenghi, Michele De Luca, Laura De Rosa
Summary: Cell therapy, gene therapy, and tissue engineering have the potential to revolutionize regenerative medicine. Gene therapy is particularly challenging but crucial for successful clinical treatment, and only a few advanced methods have been applied in routine therapy. Researchers need to address safety, efficacy, regulatory constraints, and market access in order to make gene therapy available for patients with rare diseases.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2022)
Article
Immunology
Ashrafali M. Ismail, Tiange Cui, Kalpana Dommaraju, Gurdeep Singh, Shoaleh Dehghan, Jason Seto, Susmita Shrivastava, Nadia B. Fedorova, Neha Gupta, Timothy B. Stockwell, Ramana Madupu, Albert Heim, Adriana E. Kajon, Eric G. Romanowski, Regis P. Kowalski, Jambulingam Malathi, Kuzhanthai L. Therese, Hajib Narahari Madhavan, Qiwei Zhang, Leonardo J. Ferreyra, Morris S. Jones, Jaya Rajaiya, David W. Dyer, James Chodosh, Donald Seto
EMERGING MICROBES & INFECTIONS
(2018)
Review
Microbiology
Ashrafali M. Ismail, Ji Sun Lee, Jeong Yoon Lee, Gurdeep Singh, David W. Dyer, Donald Seto, James Chodosh, Jaya Rajaiya
FRONTIERS IN MICROBIOLOGY
(2018)
Correction
Immunology
Ashrafali M. Ismail, Tiange Cui, Kalpana Dommaraju, Gurdeep Singh, Shoaleh Dehghan, Jason Seto, Susmita Shrivastava, Nadia B. Fedorova, Neha Gupta, Timothy B. Stockwell, Rebecca Halpin, Ramana Madupu, Albert Heim, Adriana E. Kajon, Eric G. Romanowski, Regis P. Kowalski, Jambulingam Malathi, Kuzhanthai L. Therese, Hajib Narahari Madhavan, Qiwei Zhang, Leonardo J. Ferreyra, Morris S. Jones, Jaya Rajaiya, David W. Dyer, James Chodosh, Donald Seto
EMERGING MICROBES & INFECTIONS
(2018)
Article
Biochemical Research Methods
Tahmid F. Mehdi, Gurdeep Singh, Jennifer A. Mitchell, Alan M. Moses
Article
Virology
Jing Zhang, June Kang, Shoaleh Dehghan, Siddharth Sridhar, Susanna K. P. Lau, Junxian Ou, Patrick C. Y. Woo, Qiwei Zhang, Donald Seto
Article
Virology
Gurdeep Singh, Ashrafali M. Ismail, Jeong Yoon Lee, Mirja Ramke, Ji Sun Lee, David W. Dyer, Donald Seto, Jaya Rajaiya, James Chodosh
Article
Virology
Shoaleh Dehghan, Jason Seto, Elizabeth B. Liu, Ashrafali M. Ismail, Ramana Madupu, Albert Heim, Morris S. Jones, David W. Dyer, James Chodosh, Donald Seto
JOURNAL OF VIROLOGY
(2019)
Article
Immunology
Kristen K. Coleman, Chui Ching Wong, Jayanthi Jayakumar, Tham T. Nguyen, Abigail W. L. Wong, Su Yadana, Koh C. Thoon, Kwai Peng Chan, Jenny G. Low, Shirin Kalimuddin, Shoaleh Dehghan, June Kang, Amirhossein Shamsaddini, Donald Seto, Yvonne C. F. Su, Gregory C. Gray
JOURNAL OF INFECTIOUS DISEASES
(2020)
Article
Evolutionary Biology
June Kang, Ashrafali Mohamed Ismail, Shoaleh Dehghan, Jaya Rajaiya, Marc W. Allard, Haw Chuan Lim, David W. Dyer, James Chodosh, Donald Seto
Article
Biochemistry & Molecular Biology
Gurdeep Singh, Shanelle Mullany, Sakthi D. Moorthy, Richard Zhang, Tahmid Mehdi, Ruxiao Tian, Alan M. Moses, Jennifer A. Mitchell, Andrew G. Duncan
Summary: Transcriptional enhancers play a critical role in development and phenotype evolution, and a diverse repertoire of TFBS sequences is necessary for constructing synthetic enhancers with comparable activity to native enhancers. A threshold of TFBS sequence diversity overrides the need for optimized regulatory grammar and specific master regulators' recruitment by individual TFBSs. At least 10 different TFBSs are required for enhancer activity, with some specific TFBSs being dispensable.
Article
Biology
Gurdeep Singh, Gilda Stefanelli, Klotilda Narkaj, Mark A. Brimble, Samantha D. Creighton, Timothy A. B. McLean, Meaghan Hall, Krista A. Mitchnick, Jacqueline Zakaria, Thanh Phung, Anas Reda, Amanda M. Leonetti, Ashley Monks, Lara Ianov, Boyer D. Winters, Brandon J. Walters, Andrew M. Davidoff, Jennifer A. Mitchell, Iva B. Zovkic
Summary: The structurally-unique histone variant mH2A1 has a greater impact on learning and memory in mice compared to a similar variant, mH2A2. mH2A1 is able to repress transcription related to memory, while mH2A2 does not have this function. This study further elucidates the role of histone variants in memory and provides insights into the underlying mechanisms.
COMMUNICATIONS BIOLOGY
(2022)
Correction
Biology
Gurdeep Singh, Gilda Stefanelli, Klotilda Narkaj, Mark A. Brimble, Samantha D. Creighton, Timothy A. B. McLean, Meaghan Hall, Krista A. Mitchnick, Jacqueline Zakaria, Thanh Phung, Anas Reda, Amanda M. Leonetti, Ashley Monks, Lara Ianov, Boyer D. Winters, Brandon J. Walters, Andrew M. Davidoff, Jennifer A. Mitchell, Iva B. Zovkic
COMMUNICATIONS BIOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Shoaleh Dehghan, Erin M. Horan, Gavin Frome
Summary: The implementation of a flipped classroom approach in organic chemistry courses can improve learning outcomes and student interest. This teaching method has been shown to increase student learning and satisfaction. However, the grade analysis did not show a significant difference between the flipped and traditional formats.
JOURNAL OF CHEMICAL EDUCATION
(2022)
Article
Microbiology
Ashrafali M. Ismail, Amrita Saha, Ji S. Lee, David F. Painter, Yinghua Chen, Gurdeep Singh, Gabriela N. Condezo, James Chodosh, Carmen San Martin, Jaya Rajaiya
Summary: In this study, the interaction between adenovirus protein pIIIa and host cellular proteins USP9x and RANBP2 was investigated. It was found that USP9x negatively regulates viral replication and protein expression, while RANBP2 is important for pIIIa transport and viral assembly. These findings have potential implications in the development of new antiviral therapeutics.
Article
Multidisciplinary Sciences
Hai-Feng Zhang, Alberto Delaidelli, Sumreen Javed, Busra Turgu, Taylor Morrison, Christopher S. Hughes, Xiaqiu Yang, Manideep Pachva, Michael M. Lizardo, Gurdeep Singh, Jennifer Hoffmann, Yue Zhou Huang, Khushbu Patel, Rawan Shraim, Sonia H. Y. Kung, Gregg B. Morin, Samuel Aparicio, Daniel Martinez, John M. Maris, Kristopher R. Bosse, Karla C. Williams, Poul H. Sorensen
Summary: This study reveals that GREB1 gene, located near MYCN locus, is frequently coexpressed with MYCN and promotes cell survival in high-risk neuroblastoma (NB). The research also identifies MYO1B gene as a crucial regulator of invasion and metastasis in MYCN amplified NB. Furthermore, MYO1B is found to regulate secretome reprogramming and the cytokine MIF mediates its pro-invasive and pro-metastatic activity.
