Article
Multidisciplinary Sciences
Qin Yu, Alba Herrero del Valle, Rahul Singh, Yorgo Modis
Summary: MDA5 is the primary immune sensor for SARS-CoV-2 and many other viruses, mutations can cause disease. This study shows how steric constraints cause MDA5 to misrecognize endogenous RNA as viral RNA, shedding light on how MDA5 recognizes dsRNA in health and disease.
NATURE COMMUNICATIONS
(2021)
Article
Immunology
Shuangjie Li, Jie Yang, Yuanyuan Zhu, Hui Wang, Xingyu Ji, Jia Luo, Qi Shao, Yulin Xu, Xueliang Liu, Wanglong Zheng, Francois Meurens, Nanhua Chen, Jianzhong Zhu
Summary: This study characterized the porcine RLR members RIG-I, MDA5, and LGP2 and found that porcine RIG-I and MDA5 exhibited similar signaling activity to distinct dsRNA and viruses compared to their human counterparts. Porcine LGP2 was found to positively regulate RIG-I and MDA5 in porcine alveolar macrophages and exert broad antiviral functions. Interestingly, mutant forms of porcine LGP2 with defects in ATPase and/or dsRNA binding showed constitutive activity likely through RIG-I and MDA5. The research provided significant insights into porcine innate immunity and immune biology.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Chemistry, Applied
Pengqi Bao, Li Chen, Yue Hu, Yan Wang, Cunliu Zhou
Summary: This study investigated the ability of L-arginine and L-lysine to mitigate the adverse effects of freezing on the structure and solubility of myofibrillar protein extract (MPE) in porcine Longissimus lumborum. The results showed that both L-arginine and L-lysine were effective in alleviating the adverse effects of freezing. These findings are important in the meat industry.
Article
Nutrition & Dietetics
Panagiota Binou, Amalia E. Yanni, Klio Kartsioti, Aikaterini Barmpagianni, Panagiotis Konstantopoulos, Vaios T. Karathanos, Alexander Kokkinos
Summary: The present study aimed to assess the impact of adding plant-based proteins with appetite-regulating properties to wheat biscuits on weight loss. Results showed that participants who consumed the protein-enriched biscuits experienced greater weight loss and improvement in metabolic parameters.
Review
Chemistry, Multidisciplinary
Sean R. Millar, Jie Qi Huang, Karl J. Schreiber, Yi-Cheng Tsai, Jiyun Won, Jianping Zhang, Alan M. Moses, Ji-Young Youn
Summary: Stress granules (SGs) are cytosolic biomolecular condensates that rapidly assemble through weak, multivalent interactions between their protein and RNA constituents. They have been implicated in cytoprotective processes and neurodegenerative diseases. In this study, a consensus SG proteome was provided and a user-friendly database for SGs was updated. The predisposition of SG proteins for phase separation and aggregation was assessed, primary sequence features of SG-resident proteins were analyzed, and determinants of SG composition and dynamics were reviewed.
Article
Chemistry, Physical
Fenfen Ma, Xiaoyan Zheng, Zesheng Li
Summary: The study using molecular dynamics simulations reveals that CB[7] binds differently to aromatic and basic residues in peptides, with the C-terminal basic residue significantly affecting the binding. Additionally, CB[7] shows efficient differentiation between peptides with similar structures, and exhibits sequence dependence in recognizing specific peptides.
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
(2021)
Article
Biochemistry & Molecular Biology
Xi Wang, Binhan Yu, Junji Iwahara
Summary: Aromatic NH2 groups are crucial for hydrogen-bond donors in the secondary structures of DNA and RNA. This study determined the kinetic rate constants and energy barriers for the rotational dynamics of cytosine NH2 groups in a 15-base-pair DNA duplex using an NMR method. The results show that the rotational dynamics of cytosine NH2 groups depend on their local environments, and there is a qualitative correlation between the ranges of N-15 chemical shifts and rotational time scales.
Editorial Material
Microbiology
Curt M. Horvath
CELL HOST & MICROBE
(2015)
Review
Biochemistry & Molecular Biology
Annie M. Bruns, Curt M. Horvath
Article
Biochemistry & Molecular Biology
Akihiko Komuro, Yuya Homma, Takaharu Negoro, Glen N. Barber, Curt M. Horvath
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2016)
Article
Microbiology
Darja Pollpeter, Maddy Parsons, Andrew E. Sobala, Sashika Coxhead, Rupert D. Lang, Annie M. Bruns, Stelios Papaioannou, James M. McDonnell, Luis Apolonia, Jamil A. Chowdhury, Curt M. Horvath, Michael H. Malim
NATURE MICROBIOLOGY
(2018)
Article
Biochemistry & Molecular Biology
Jean-Patrick Parisien, Jessica J. Lenoir, Roli Mandhana, Kenny R. Rodriguez, Kenin Qian, Annie M. Bruns, Curt M. Horvath
Article
Biochemistry & Molecular Biology
Roli Mandhana, Lily K. Qian, Curt M. Horvath
JOURNAL OF INTERFERON AND CYTOKINE RESEARCH
(2018)
Review
Biochemistry & Molecular Biology
Nancy Au-Yeung, Curt M. Horvath
CYTOKINE & GROWTH FACTOR REVIEWS
(2018)
Article
Biochemistry & Molecular Biology
Diana Saleiro, Gavin T. Blyth, Ewa M. Kosciuczuk, Patrick A. Ozark, Beata Majchrzak-Kita, Ahmet D. Arslan, Mariafausta Fischietti, Neha K. Reddy, Curt M. Horvath, Roger J. Davis, Eleanor N. Fish, Leonidas C. Platanias
Article
Multidisciplinary Sciences
Roli Mandhana, Curt M. Horvath
SCIENTIFIC REPORTS
(2018)
Article
Biochemistry & Molecular Biology
Kendra M. Quicke, Kristin Y. Kim, Curt M. Horvath, Mehul S. Suthar
JOURNAL OF INTERFERON AND CYTOKINE RESEARCH
(2019)
Article
Multidisciplinary Sciences
Rogan A. Grant, Luisa Morales-Nebreda, Nikolay S. Markov, Suchitra Swaminathan, Melissa Querrey, Estefany R. Guzman, Darryl A. Abbott, Helen K. Donnelly, Alvaro Donayre, Isaac A. Goldberg, Zasu M. Klug, Nicole Borkowski, Ziyan Lu, Hermon Kihshen, Yuliya Politanska, Lango Sichizya, Mengjia Kang, Ali Shilatifard, Chao Qi, Jon W. Lomasney, A. Christine Argento, Jacqueline M. Kruser, Elizabeth S. Malsin, Chiagozie O. Pickens, Sean B. Smith, James M. Walter, Anna E. Pawlowski, Daniel Schneider, Prasanth Nannapaneni, Hiam Abdala-Valencia, Ankit Bharat, Cara J. Gottardi, G. R. Scott Budinger, Alexander Misharin, Benjamin D. Singer, Richard G. Wunderink
Summary: The study found that patients infected with SARS-CoV-2 have enriched T cells and monocytes in the alveolar space, suggesting that the virus infects alveolar macrophages and induces T cell production of interferon-, leading to inflammation and persistent alveolitis.
Article
Cell Biology
Jessica J. Lenoir, Jean-Patrick Parisien, Curt M. Horvath
Summary: The study reveals that LGP2 inhibits K63-Ub chains by associating with and sequestering the K63-Ub-conjugating enzyme Ubc13/UBE2N, thereby affecting the regulation of a range of K63-Ub ligase proteins and immune signaling pathways.
Article
Virology
Curt M. Horvath, Rebecca E. Dutch, Charles J. Russell
JOURNAL OF VIROLOGY
(2023)
Article
Virology
Jean-Patrick Parisien, Jessica J. Lenoir, Gloria Alvarado, Curt M. Horvath
Summary: The ability of Zika virus to degrade STAT2 protein in order to evade the host immune response is investigated in this study. The interaction between NS5 and STAT2 is found to be essential for proteasome-mediated degradation of STAT2. This research provides new insights into the mechanisms of Zika virus evasion and identifies potential targets for antiviral therapies.
JOURNAL OF VIROLOGY
(2022)
Article
Multidisciplinary Sciences
Nancy Au-Yeung, Curt M. Horvath