Article
Microbiology
Ye Liu, Qiao You, Fang Zhang, Deyan Chen, Zhenping Huang, Zhiwei Wu
Summary: Harringtonine (HT) significantly inhibited HSV-1 infection and two ACV-resistant strains by mainly targeting HVEM and reducing the early stage of HSV-1 infection. The study demonstrated that HT could be a promising therapeutic candidate for mitigating HSV-1-induced pathogenesis.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Microbiology
James P. Cwick, Jonathan E. Owen, Irina Kochetkova, Kyle S. Hain, Nick Van Horssen, Matthew P. Taylor
Summary: Most viruses utilize superinfection exclusion (SIE) to conserve resources and control population dynamics. This study provides new insights into how alphaherpesviruses limit sequential infection through the undercharacterized gD-independent SIE, which differs between PRV and HSV-1.
MICROBIOLOGY SPECTRUM
(2022)
Article
Virology
Qing Fan, Richard Longnecker, Sarah A. Connolly
Summary: The viral fusion protein glycoprotein B (gB) is essential for virus entry in all herpesviruses. By introducing structure-based mutations, researchers identified mutations that counteracted a slow-entry phenotype in the virus and found that these mutations were located in different regions of the gB protein.
JOURNAL OF VIROLOGY
(2021)
Review
Pharmacology & Pharmacy
Yiwei Huang, Yuyun Song, Jichen Li, Changning Lv, Zhe-Sheng Chen, Zhijun Liu
Summary: Human herpes simplex viruses use cell surface receptors such as heparan sulfate, herpesvirus entry mediator, and nectin-1/-2, 3-O-sulfated heparan sulfate to enter host cells. No vaccine or curative treatment is currently available for HSV infections.
DRUG DISCOVERY TODAY
(2022)
Article
Virology
Mashidur Rana, Mizumi Setia, Pratima K. Suvas, Anish Chakraborty, Susmit Suvas
Summary: Reactive oxygen species (ROS) play a crucial role in herpes stromal keratitis (HSK), with neutrophils being the primary cells responsible for ROS generation. NOX2 is involved in ROS production in neutrophils. However, manipulating ROS levels in neutrophils may help alleviate the severity of HSK, and NOX2 is not the main contributor to ROS generation in neutrophils.
JOURNAL OF VIROLOGY
(2022)
Article
Microbiology
Qing Fan, Daniel P. Hippler, Yueqi Yang, Richard Longnecker, Sarah A. A. Connolly
Summary: This study reveals that two mutations in gH (H789Y and S830N) contribute to the activation of gB during herpes simplex virus 1 (HSV-1) entry into cells. These findings provide valuable insights into the functional interactions between gH/gL and gB.
Article
Microbiology
Kevin Danastas, Gerry Guo, Jessica Merjane, Nathan Hong, Ava Larsen, Monica Miranda-Saksena, Anthony L. Cunningham
Summary: This study explores the effects of interferons (IFNs) on herpes simplex virus-1 (HSV-1) and reveals that IFNs have the potential to block virus release from nerve endings, thereby preventing transmission into the skin. The study also highlights the potential wider antiviral effects of IFN-γ in neurons, suggesting its role in HSV-1 reactivation. These findings identify new targets for the development of immunotherapies to impede HSV-1 spread from nerves to the skin.
Article
Biochemistry & Molecular Biology
Bishajit Sarkar, Md. Asad Ullah, Yusha Araf, Sowmen Das, Md. Hasanur Rahman, Abu Tayab Moin
Summary: HSV is a highly infectious virus with two types, HSV-1 and HSV-2, infecting millions worldwide. This study designed three polyvalent subunit vaccines targeting multiple strains of HSV, which showed stability and potential effectiveness through molecular docking and simulation studies. Validation of these vaccines in wet lab-based studies is still needed.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2021)
Article
Microbiology
Xiaowei Song, Yiliang Wang, Feng Li, Wenyan Cao, Qiongzhen Zeng, Shurong Qin, Zhaoyang Wang, Jiaoyan Jia, Ji Xiao, Xiao Hu, Kaisheng Liu, Yifei Wang, Zhe Ren
Summary: This study found that inhibitors of heat shock protein 90 (Hsp90) have antiviral activity against HSV-1 infection in neuronal cells. The results suggest that Hsp90 inhibitors promote viral adsorption but inhibit subsequent penetration, providing further insights into the molecular mechanism of Hsp90 in HSV-1 infection.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Virology
Marianne Boutin, Halima Medjahed, Manon Nayrac, Rishikesh Lotke, Gabrielle Gendron-Lepage, Catherine Bourassa, Daniel Sauter, Jonathan Richard, Andres Finzi
Summary: HIV-1 envelope glycoproteins (Envs) play a crucial role in viral entry and are a preferred target for small molecule inhibitors. Temsavir inhibits the interaction between the host cell receptor CD4 and Env by binding a specific region on the Env subunit gp120. Besides blocking viral entry, temsavir also stabilizes the closed conformation of Env. This study shows that temsavir affects the glycosylation, proteolytic processing, and overall conformation of Env, which in turn impacts the recognition of HIV-1-infected cells by broadly neutralizing antibodies and their ability to mediate antibody-dependent cellular cytotoxicity (ADCC).
Article
Virology
Katrina A. Gianopulos, Tri Komala Sari, Darin J. Weed, Suzanne M. Pritchard, Anthony Nicola
Summary: Low endosomal pH facilitates herpesvirus entry in a cell-specific manner. The gC protein of HSV-1 plays a critical role in low-pH entry pathway by regulating the conformational changes of viral envelope glycoprotein B (gB). This study reveals that low pH triggers conformational changes in gC itself, and a specific gC epitope is important for low-pH entry inhibition.
JOURNAL OF VIROLOGY
(2022)
Article
Pathology
Melissa Krystel-Whittemore, May P. Chan, Sara C. Shalin, Kenan J. Sauder, Amy Hudson, Ruth K. Foreman, Mai P. Hoang, Jeoffry B. Brennick, Shaofeng Yan, Rosalynn M. Nazarian
Summary: This study presents the first known report of herpes virus infecting deep stromal cells of the dermis, highlighting the importance of considering cutaneous stromal herpes in patients with atypical clinical lesions, especially when immunocompromised. Establishing the correct diagnosis is crucial for initiating therapy.
AMERICAN JOURNAL OF SURGICAL PATHOLOGY
(2021)
Review
Virology
Nithya Jambunathan, Carolyn M. Clark, Farhana Musarrat, Vladimir N. Chouljenko, Jared Rudd, Konstantin G. Kousoulas
Summary: HSV-1 and HSV-2 are prototypical alphaherpesviruses that infect neurons and establish lifelong latent infections. They cause orofacial and ocular infections, genital and neonatal infections respectively. The viral glycoproteins bind to cellular receptors to enter cells and mediate cell-to-cell fusion for virus spread. The complex of viral glycoproteins and cellular receptors play a critical role in virus entry and spread.
Article
Dermatology
Deepthi Konda, Laxmisha Chandrashekar, Rahul Dhodapkar, Rajesh Nachiappa Ganesh, Devinder Mohan Thappa
Summary: This study characterized the clinical markers of herpes simplex virus (HSV) infection among patients with pemphigus vulgaris. It was found that male sex, presence of fissures, hemorrhagic crusts, erosions with angulated margins, linear erosions, and raised erythrocyte sedimentation rate were significantly associated with HSV infection. Hemorrhagic crusts and linear erosions were identified as independent predictors of HSV infection.
JOURNAL OF THE AMERICAN ACADEMY OF DERMATOLOGY
(2023)
Article
Virology
Carmen Elena Gonzalez, Nawel Ben Abdeljelil, Angela Pearson
Summary: UL24 of HSV-1 plays an important role in virus infection, and its C-terminal domain regulates its nuclear and cytoplasmic localization during infection. Mutations can enhance the accumulation of UL24 in the nucleus, and specific inhibitors can block the nuclear export of UL24.
Article
Immunology
David Bernstein, Derek A. Pullum, Rhonda D. Cardin, Fernando J. Bravo, David A. Dixon, Konstantin G. Kousoulas
Article
Veterinary Sciences
Natalie Fowlkes, Kelli Clemons, Paul J. F. Rider, Ramesh Subramanian, Nobuko Wakamatsu, Ingeborg Langohr, Konstantin G. Kousoulas
COMPARATIVE MEDICINE
(2019)
Article
Multidisciplinary Sciences
David I. Bernstein, Rhonda D. Cardin, Derek A. Pullum, Fernando J. Bravo, Konstantin G. Kousoulas, David A. Dixon
Article
Cell Biology
Jean Christopher Chamcheu, Stephane Esnault, Vagar M. Adhami, Andrea L. Noll, Sergette Banang-Mbeumi, Tithi Roy, Sitanshu S. Singh, Shile Huang, Konstantin G. Kousoulas, Hasan Mukhtar
Article
Genetics & Heredity
Andrew C. Lewin, Lyndon M. Coghill, Gillian J. McLellan, Ellison Bentley, Konstantin G. Kousoulas
Article
Multidisciplinary Sciences
Paul J. F. Rider, Lyndon M. Coghill, Misagh Naderi, Jeremy M. Brown, Michal Brylinski, Konstantin G. Kousoulas
SCIENTIFIC REPORTS
(2019)
Article
Multidisciplinary Sciences
Shan K. Naidu, Rafiq Nabi, Nagarjuna R. Cheemarla, Brent A. Stanfield, Paul J. Rider, Nithya Jambunathan, Vladimir N. Chouljenko, Renee Carter, Fabio Del Piero, Ingeborg Langohr, Konstantin G. Kousoulas
Article
Virology
Farhana Musarrat, Vladimir Chouljenko, Achyut Dahal, Rafiq Nabi, Tamara Chouljenko, Seetharama D. Jois, Konstantin G. Kousoulas
JOURNAL OF MEDICAL VIROLOGY
(2020)
Article
Virology
Farhana Musarrat, Vladimir Chouljenko, Konstantin G. Kousoulas
Summary: Herpes simplex virus 1 utilizes cellular motor proteins and kinase pathways for intracellular virion transport, with Akt and kinase C inhibitors blocking virus entry and virion capsid transport. UL37 and Vp5 viral proteins are crucial for dynein-dependent transport of virion capsids, while Akt and kinase C do not directly phosphorylate or activate dynein during infection.
JOURNAL OF VIROLOGY
(2021)
Article
Biochemistry & Molecular Biology
Tithi Roy, Samuel T. Boateng, Sergette Banang-Mbeumi, Pankaj K. Singh, Pratik Basnet, Roxane-Cherille N. Chamcheu, Federico Ladu, Isabel Chauvin, Vladimir S. Spiegelman, Ronald A. Hill, Konstantin G. Kousoulas, Bolni Marius Nagalo, Anthony L. Walker, Jean Fotie, Siva Murru, Mario Sechi, Jean Christopher Chamcheu
Summary: Novel flavonol-based derivatives targeting skin cancers were synthesized and characterized, showing significant inhibitory effects on human skin cancer cell lines. These compounds were found to induce apoptosis of cancer cells by regulating key molecular targets, presenting promising therapeutic potential for melanoma and non-melanoma skin cancers.
BIOORGANIC CHEMISTRY
(2021)
Article
Virology
Jared S. Rudd, Farhana Musarrat, Konstantin G. Kousoulas
Summary: BoHV-1 infects bovine-derived neuronal cells and can be efficiently studied using FBBC-1 cells. The study provides insights into virus entry and spread mechanisms in a neuronal cell culture system.
Review
Virology
Ifeanyi Kingsley Uche, Konstantin G. Kousoulas, Paul J. F. Rider
Summary: The development of cancer disrupts anti-tumor immunity needed for surveillance and elimination of tumor cells, with immunotherapy aiming to restore or establish these responses. However, a significant proportion of patients do not respond equally to immunotherapies, partially due to the immunosuppressive tumor microenvironment. Current research focuses on mechanisms of immunosuppression in the TME, with the goal of promoting anti-tumor immune responses.
Article
Multidisciplinary Sciences
Tithi Roy, Samuel T. Boateng, Sergette Banang-Mbeumi, Pankaj K. Singh, Pratik Basnet, Roxane-Cherille N. Chamcheu, Federico Ladu, Isabel Chauvin, Vladimir S. Spiegelman, Ronald A. Hill, Konstantin G. Kousoulas, Bolni Marius Nagalo, Anthony L. Walker, Jean Fotie, Siva Murru, Mario Sechi, Jean Christopher Chamcheu
Summary: This study identifies a panel of novel fisetin analogs that may be developed as potential therapeutic agents for melanoma and non-melanoma skin cancers, providing in-depth biological characterization data to support further research and experimental screening.
Article
Virology
Ifeanyi Kingsley Uche, Natalie Fowlkes, Luan Vu, Tatiane Watanabe, Mariano Carossino, Rafiq Nabi, Fabio del Piero, Jared S. Rudd, Konstantin G. Kousoulas, Paul J. F. Rider
Summary: Oncolytic virotherapy (OVT) uses viral infection to release tumor antigens, promoting antitumor immune responses. VC2, a modified herpes simplex virus, shows clinical potential in a mouse melanoma model and may be an effective treatment for human and animal cancers.
JOURNAL OF VIROLOGY
(2021)
Article
Microbiology
Paul J. F. Rider, Ifeanyi K. Uche, Larissa Sweeny, Konstantin G. Kousoulas
CURRENT CLINICAL MICROBIOLOGY REPORTS
(2019)