Article
Biochemistry & Molecular Biology
Shulong Zu, Chunfeng Li, Lili Li, Yong-Qiang Deng, Xiang Chen, Dan Luo, Qing Ye, Yi-Jiao Huang, Xiao-Feng Li, Rong-Rong Zhang, Nina Sun, Xianqi Zhang, Saba R. Aliyari, Karin Nielsen-Saines, Jae U. Jung, Heng Yang, Cheng-Feng Qin, Genhong Cheng
Summary: TRIM22, an ISG, plays a crucial role in host defense against flaviviruses by binding and degrading NS1 and NS3 proteins.
CELL AND BIOSCIENCE
(2022)
Article
Microbiology
Jasmine Moshiri, Ailsa R. R. Craven, Sara B. B. Mixon, Manuel R. R. Amieva, Karla Kirkegaard
Summary: Infected cells in the human colon tissue are extruded through a mechanism that depends on mechanical force, which helps maintain the integrity of the uninfected host epithelium. In the case of Enterovirus A71, infected cells are specifically extruded from the apical surface of differentiated human colon organoids using mechanosensitive ion channels, rather than undergoing apoptotic cell death. These extruded cells can be used as a source of virus for new infections.
NATURE MICROBIOLOGY
(2023)
Article
Microbiology
Wei Wu, Yang Qu, Shengqing Yu, Sa Wang, Yuncong Yin, Qinfang Liu, Chunchun Meng, Ying Liao, Zaib Ur Rehman, Lei Tan, Cuiping Song, Xusheng Qiu, Weiwei Liu, Chan Ding, Yingjie Sun
Summary: DDX21 is identified as a key regulator of immune balance, with virus infection and RNA/DNA ligands leading to its cleavage and suppression of the interferon beta signaling pathway. Modulating DDX21 cleavage may impact the innate immune response to viruses.
Article
Engineering, Chemical
Bo Gong, Wei Chen, Chen Qian, Patrick H. -L. Sit, Xian-Wei Liu, Han-Qing Yu
Summary: Membrane fouling caused by contaminants such as proteins is a common problem in water and wastewater treatment using membrane-based filtration. However, the contribution of protein-like fractions towards ceramic membrane fouling is not well understood. This study investigated the fouling behaviors of intact proteins (BSA) and peptide mixtures (tryptones) in a ceramic membrane microfiltration system, as well as the fouling behaviors of environmental protein-like substances (EP) extracted from different sludge sources. The results showed that both model and actual protein fractions led to light membrane fouling, indicating that proteins were not the main cause of membrane fouling at the early stage of ceramic membrane microfiltration.
SEPARATION AND PURIFICATION TECHNOLOGY
(2023)
Article
Multidisciplinary Sciences
Chenxu Guo, Shang-Jul Tsai, Yiwei Ai, Maggie Li, Eduardo Anaya, Andrew Pekosz, Andrea Cox, Stephen J. Gould
Summary: The D614G mutation in SARS-CoV-2 enhances Spike protein trafficking to lysosomes, reprograms lysosomes, and reduces cell surface Spike expression. This mutation is not specific to humans, but rather an adaptation to an earlier furin cleavage site insertion mutation. The D614G mutation restores Spike trafficking to lysosomes and enhances the earliest events in SARS-CoV-2 infectivity.
Article
Virology
Linyuan Yu, Yeqing Cao, Sicheng Ge, Anying Xu, Heying Qian, Gang Li
Summary: In this study, comparative transcriptome analyses were conducted to identify early-stage transcriptional changes in resistant and susceptible silkworms infected with BmNPV. Several differentially expressed genes were found, including four genes that were downregulated in the resistant variety and upregulated in the susceptible variety. These genes could potentially play a role in the resistance against early-stage viral replication. This study provides new insights into the mechanism of BmNPV infection in silkworms and potential treatment options.
Article
Biochemistry & Molecular Biology
Markus Hoffmann, Prerna Arora, Rudiger Gross, Alina Seidel, Bojan F. Hornich, Alexander S. Hahn, Nadine Kruger, Luise Graichen, Heike Hofmann-Winkler, Amy Kempf, Martin S. Winkler, Sebastian Schulz, Hans-Martin Jack, Bernd Jahrsdorfer, Hubert Schrezenmeier, Martin Muller, Alexander Kleger, Jan Munch, Stefan Pohlmann
Summary: The emerging SARS-CoV-2 variants may exhibit resistance to existing neutralizing antibodies and treatments, which could have significant implications for pandemic containment efforts.
Article
Immunology
Tiecheng Wang, Faming Miao, Shengnan Lv, Liang Li, Feng Wei, Lihua Hou, Renren Sun, Wei Li, Jian Zhang, Cheng Zhang, Guang Yang, Haiyang Xiang, Keyin Meng, Zhonghai Wan, Busen Wang, Guodong Feng, Zhongpeng Zhao, Deyan Luo, Nan Li, Changchun Tu, Hui Wang, Xiaochang Xue, Yan Liu, Yuwei Gao
Summary: This study established a COVID-19 infection model in cynomolgus macaques and analyzed the underlying pathogenic mechanisms of moderate cases using proteomic and metabolomic profiling. The results showed dysregulation of innate immune response, neutrophil and platelet activation in the COVID-19 macaque models. The symptoms of neutrophilia, lymphopenia, and cytokine storm, which are typically seen in severe cases, were significantly attenuated in most of the macaques, resembling moderate cases. The macaque model could be a valuable tool for understanding the pathogenesis of moderate COVID-19 and evaluating the safety and efficacy of therapeutics and vaccines.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Microbiology
Ruixuan Zhang, Hisashi Endo, Masaharu Takemura, Hiroyuki Ogata
Summary: Medusavirus, an amoeba-infecting giant virus, has a unique replication cycle and infection strategy compared to other NCLDVs. Through RNA-seq analysis, the ordered viral gene expression profiles and the impact on host nuclear environment during infection were revealed. The transcriptional profile of amoeba genes was significantly altered postinfection, with increased representation of encystment-related host genes.
MICROBIOLOGY SPECTRUM
(2021)
Article
Multidisciplinary Sciences
Mayuko Yagi, Minami Hama, Sayaka Ichii, Yurie Nakashima, Daiki Kanbayashi, Takako Kurata, Kosuke Yusa, Jun Komano
Summary: This study used CRISPR-Cas9 system to conduct a loss-of-function screening in human placenta-derived JAR cells, and identified sphingomyelin synthase 1 as a susceptibility factor for rubella virus infection. The study revealed the unique regulation of rubella virus replication by a host factor during the early and late phases of the viral life cycle.
Article
Multidisciplinary Sciences
Juan A. De la Cruz, Thota Ganesh, Becky A. Diebold, Weiping Cao, Amelia Hofstetter, Neetu Singh, Amrita Kumar, James McCoy, Priya Ranjan, Susan M. E. Smith, Suryaprakash Sambhara, J. David Lambeth, Shivaprakash Gangappa
Summary: TG6-44 can significantly reduce ROS and inflammatory mediators induced by influenza A virus infection, demonstrate antiviral effects in cells, and lead to decreased virus-induced cell death.
Article
Plant Sciences
Yan Zhang, Chuanxi Zhang, Junmin Li, Jianping Chen, Gang Lu
Summary: This study discovered that the 14K protein encoded by wheat yellow mosaic virus can induce systemic necrosis in plants and suppress the replication of other viruses.
PHYTOPATHOLOGY RESEARCH
(2022)
Article
Multidisciplinary Sciences
Lei Guo, Yan Liang, Heng Li, Huiwen Zheng, Zening Yang, Yanli Chen, Xin Zhao, Jing Li, Binxiang Li, Haijing Shi, Ming Sun, Longding Liu
Summary: Glycosylation of the SARS-CoV-2 spike glycoprotein is crucial for viral entry and immune evasion, with different glycan patterns affecting binding affinities to C-type lectin receptors. Although C-type lectin receptors do not support productive viral infection, they play a significant role in viral trans and cis-infection, especially with virions containing a high-mannose structure at specific glycan sites. This highlights the importance of viral epigenetic glycosylation in authentic viral infection and the co-receptor role of DC&L-SIGN in SARS-CoV-2 infection and prevention.
Article
Multidisciplinary Sciences
Joan B. Soriano, Adrian Pelaez, Xavier Busquets, Maria Rodrigo-Garcia, Elena Avalos Perez-Urria, Tamara Alonso, Rosa Giron, Claudia Valenzuela, Celeste Marcos, Elena Garcia-Castillo, Julio Ancochea
Summary: The association between ABO blood group and COVID-19 remains controversial. However, this study found no significant correlation between ABO blood groups and either COVID-19 or Long COVID.
Article
Gastroenterology & Hepatology
Xiaohui Zhang, Yingying Jiang, Shaobin Li, Dandan Bian, Mei Liu, Ming Kong, Yu Chen, Zhongping Duan, Sujun Zheng
Summary: After DAA treatment, NK cell cytotoxicity initially recovers in the early stage of treatment, but then continuously decreases to below normal levels. Changes in NK cell subsets and phenotypes are closely related to NK function.
JOURNAL OF CLINICAL AND TRANSLATIONAL HEPATOLOGY
(2022)
Article
Fisheries
Thavasimuthu Citarasu, Chinnadurai Lelin, Eswaramoorthy Thirumalaikumar, Mariavincent Michael Babu, Vikram N. Vakharia
FISH & SHELLFISH IMMUNOLOGY
(2019)
Article
Fisheries
Thavasimuthu Citarasu, Chinnadurai Lelin, Mariavincent Michael Babu, Setty Balakrishnan Anand, Abel Arul Nathan, Vikram N. Vakharia
FISH & SHELLFISH IMMUNOLOGY
(2019)
Article
Virology
Shelby Powell Kesterson, Jeffery Ringiesn, Vikram N. Vakharia, Brian S. Shepherd, Douglas W. Leaman, Krishnamurthy Malathi
Article
Microbiology
Ashis Debnath, Dinesh C. Pathak, Ajai Lawrence D'silva, Rahul Batheja, Narayan Ramamurthy, Vikram N. Vakharia, Madhan Mohan Chellappa, Sohini Dey
VETERINARY MICROBIOLOGY
(2020)
Article
Immunology
Changyong Mu, Qiwang Zhong, Yan Meng, Yong Zhou, Nan Jiang, Wenzhi Liu, Yiqun Li, Mingyang Xue, Lingbing Zeng, Vikram N. Vakharia, Yuding Fan
Summary: In this study, a recombinant baculovirus expressing VP35-VP4 proteins from GCRV was generated and used to vaccinate grass carp through oral administration. The results showed that the vaccine induced both non-specific and specific immune responses, providing protection against GCRV infection with a relative percent survival rate of 56%. This study suggests that the silkworm pupae powder could be used as an effective oral vaccine for grass carp.
Article
Virology
Ke Zhang, Wenzhi Liu, Yiqun Li, Yong Zhou, Yan Meng, Lingbing Zeng, Vikram N. Vakharia, Yuding Fan
Summary: A new grass carp reovirus, named healthy grass carp reovirus (HGCRV), was isolated from grass carp in 2019. Comparisons showed that it was closely related to GCRV-873 (GCRV-I), but distantly related to other known GCRVs.
Article
Veterinary Sciences
Narayan Ramamurthy, Dinesh C. Pathak, Ajai Lawrence D'Silva, Rahul Batheja, Asok Kumar Mariappan, Vikram N. Vakharia, Madhan Mohan Chellappa, Sohini Dey
Summary: The study demonstrated that the R2B-GFP virus significantly reduced the viability of infected 4T1 and B16-F10 cells, and induced apoptosis in these cells compared to the uninfected controls. Additionally, the R2B-GFP virus increased mitochondrial membrane permeability in the cells, suggesting the involvement of mitochondrial-regulated cell death.
RESEARCH IN VETERINARY SCIENCE
(2021)
Article
Virology
Maria Soderlund-Venermo, Anupam Varma, Deyin Guo, Douglas P. Gladue, Emma Poole, Flor H. Pujol, Hanu Pappu, Jesus L. Romalde, Laura Kramer, Mariana Baz, Marietjie Venter, Matthew D. Moore, Michael M. Nevels, Sayeh Ezzikouri, Vikram N. Vakharia, William C. Wilson, Yashpal S. Malik, Zhengli Shi, Ahmed S. Abdel-Moneim
Summary: This article summarizes the presentations given at the 1st international conference of the World Society for Virology (WSV). The conference focused on tackling global viral epidemics and aimed to foster international collaborations. The conference consisted of multiple sessions and attracted 270 scientists from 49 countries.
Article
Virology
Madhan Mohan Chellappa, Sohini Dey, Dinesh Chandra Pathak, Asmita Singh, Narayan Ramamurthy, Saravanan Ramakrishnan, Asok Kumar Mariappan, Kuldeep Dhama, Vikram N. Vakharia
Summary: The study utilized a Newcastle disease virus (NDV) strain as a viral vector to deliver immunogenic genes of chicken infectious anaemia virus (CIAV), resulting in a bivalent vaccine candidate for chickens. The vaccine was able to induce robust humoral and cell-mediated immune responses in chickens, showing potential for effective protection against NDV and CIAV infections.
Article
Multidisciplinary Sciences
Gina R. Castro-Sanguinetti, Paulo Vitor Marques Simas, Ana Paola Apaza-Chiara, Jose Alonso Callupe-Leyva, Juan Alexander Rondon-Espinoza, Cesar M. Gavidia, Juan Anderson More-Bayona, Rosa Isabel Gonzalez Veliz, Vikram N. Vakharia, Maria Eliana Icochea
Summary: This study aimed to monitor avian influenza viruses (AIVs) in wild birds from Peru. Several low-pathogenic AIVs were isolated from collected fecal samples, with one isolate showing a higher genetic relationship to Chilean strains. The results highlight the importance of geographical factors in the evolution of AIV.
Article
Multidisciplinary Sciences
Manolo Fernandez Diaz, Katherine Calderon, Aldo Rojas-Neyra, Vikram N. Vakharia, Ricardo Choque-Guevara, Angela Montalvan-Avalos, Astrid Poma-Acevedo, Dora Rios-Matos, Andres Agurto-Arteaga, Maria De Grecia Cauti-Mendoza, Norma Perez-Martinez, Gisela Isasi-Rivas, Luis Tataje-Lavanda, Yacory Sernaque-Aguilar, Freddy Ygnacio, Manuel Criollo-Orozco, Edison Huaccachi-Gonzalez, Elmer Delgado-Ccancce, Doris Villanueva-Perez, Ricardo Montesinos-Millan, Kristel Gutierrez-Manchay, Katherinne Pauyac-Antezana, Ingrid Ramirez-Ortiz, Stefany Quinones-Garcia, Yudith Cauna-Orocollo, Katherine Vallejos-Sanchez, Angela Rios-Angulo, Dennis Nunez-Fernandez, Mario Salguedo-Bohorquez, Julio Ticona, Manolo Fernandez-Sanchez, Eliana Icochea, Luis A. Guevara-Sarmiento, Mirko Zimic
Summary: This study developed three recombinant Newcastle disease virus (rNDV) vectored vaccines and demonstrated their effectiveness in generating neutralizing antibodies, preventing lung damage, and reducing viral load in animals. The results indicate the potential of these vaccines as a promising tool in fighting against COVID-19.
SCIENTIFIC REPORTS
(2022)
Article
Fisheries
Xiaodan Liu, Liwen Zhang, Yanbing Zhang, Vikram N. Vakharia, Xiaojun Zhang, Xiaoyang Lv, Wei Sun
Summary: This study investigates the interaction between snakehead ISG15 and other proteins using a yeast expression system, and identifies multiple interacting proteins. These results are important for further exploration of the function and mechanism of snakehead ISG15.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Fisheries
Xiaodan Liu, Yanbing Zhang, Zheling Zhang, Zhenhua An, Xiaojun Zhang, Vikram N. Vakharia, Li Lin
Summary: A highly pathogenic LMBV-like virus was isolated and identified from juvenile largemouth bass. Infected fish showed typical signs of acute haemorrhages and visceral enlargement. The virus also significantly enhanced the immunological activity of the juvenile largemouth bass.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Fisheries
Bartolomeo Gorgoglione, Juan-Ting Liu, Jie Li, Vikram N. Vakharia
Summary: Recombinant baculoviruses expressing IHNV G and IHNV G-C5a proteins were used to produce an oral vaccine for rainbow trout. The vaccine showed some level of antiviral protection, but there was no significant difference in viral load between the experimental groups. Further study is needed to evaluate the modulation of immune response by the oral vaccine.
FISH AND SHELLFISH IMMUNOLOGY REPORTS
(2023)
Article
Microbiology
Changyong Mu, Vikram N. Vakharia, Yong Zhou, Nan Jiang, Wenzhi Liu, Yan Meng, Yiqun Li, Mingyang Xue, Jieming Zhang, Lingbing Zeng, Qiwang Zhong, Yuding Fan
