Article
Immunology
Cooper K. Hayes, Christopher K. Villota, Fiona B. McEnany, Stacey Ceron, Sita Awasthi, Moriah L. Szpara, Harvey M. Friedman, David A. Leib, Richard Longnecker, Matthew D. Weitzman, Lisa N. Akhtar
Summary: Clinical HSV-2 isolates collected from neonates with encephalitis are more neurovirulent in human neuronal cell culture and murine models as compared to isolates from neonates with skin-limited disease, suggesting that viral factors contribute to neurologic outcome following human neonatal infection.
JOURNAL OF INFECTIOUS DISEASES
(2022)
Article
Biochemistry & Molecular Biology
Bishajit Sarkar, Md. Asad Ullah, Yusha Araf, Sowmen Das, Md. Hasanur Rahman, Abu Tayab Moin
Summary: HSV is a highly infectious virus with two types, HSV-1 and HSV-2, infecting millions worldwide. This study designed three polyvalent subunit vaccines targeting multiple strains of HSV, which showed stability and potential effectiveness through molecular docking and simulation studies. Validation of these vaccines in wet lab-based studies is still needed.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2021)
Review
Biochemistry & Molecular Biology
Ines Ripa, Sabina Andreu, Jose Antonio Lopez-Guerrero, Raquel Bello-Morales
Summary: The article discusses the role of the HSV-1 viral protein ICP34.5 in controlling cellular antiviral responses and debates its controversial functions such as autophagy inhibition.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Pharmacology & Pharmacy
Yiwei Huang, Yuyun Song, Jichen Li, Changning Lv, Zhe-Sheng Chen, Zhijun Liu
Summary: Human herpes simplex viruses use cell surface receptors such as heparan sulfate, herpesvirus entry mediator, and nectin-1/-2, 3-O-sulfated heparan sulfate to enter host cells. No vaccine or curative treatment is currently available for HSV infections.
DRUG DISCOVERY TODAY
(2022)
Article
Virology
Michiel van Gent, Jessica J. Chiang, Santoshi Muppala, Cindy Chiang, Walid Azab, Lisa Kattenhorn, David M. Knipe, Nikolaus Osterrieder, Michaela U. Gack
Summary: Recent studies show that herpes simplex virus 1 (HSV-1) can evade the immune response by phosphorylating the cytosolic pathogen sensor RIG-I, keeping it in a signaling-repressed state.
JOURNAL OF VIROLOGY
(2022)
Correction
Multidisciplinary Sciences
Rosamaria Pennisi, Maria Musarra-Pizzo, Zhixiang Lei, Grace Guoying Zhou, Maria Teresa Sciortino
Summary: The paper has been amended and the revised version can be accessed through a link at the top of the paper.
SCIENTIFIC REPORTS
(2021)
Article
Multidisciplinary Sciences
Lanxin Li, Inge Verstraeten, Mark Roosjen, Koji Takahashi, Lesia Rodriguez, Jack Merrin, Jian Chen, Lana Shabala, Wouter Smet, Hong Ren, Steffen Vanneste, Sergey Shabala, Bert De Rybel, Dolf Weijers, Toshinori Kinoshita, William M. Gray, Jiri Friml
Summary: Plant growth is regulated by environmental factors, such as how plants respond to gravity by bending shoots up and roots down. Research has shown that the plant hormone auxin affects root growth by regulating cell expansion and contraction in the roots.
Article
Virology
Huxley K. Hoffman, Rebekah S. Aguilar, Austin R. Clark, Nicholas S. Groves, Nairi Pezeshkian, Merissa M. Bruns, Schuyler B. van Engelenburg
Summary: This study investigates the trafficking pathways of internalized HIV-1 envelope glycoprotein (Env) in infected CD4(+) T cells and the role of host cell Rab GTPases. The findings suggest that internalized Env traffics to Rab14(+) compartments that resemble late endosomes and lysosomes. Additionally, the study demonstrates that Env can be recycled back to the plasma membrane.
JOURNAL OF VIROLOGY
(2022)
Article
Infectious Diseases
Zhaoqi Wang, Han Yan, Fujie He, Jie Wang, Yang Zhang, Lishan Sun, Cui Hao, Wei Wang
Summary: The natural compound wedelolactone (WDL) exhibits strong inhibitory effects against both HSV-1 and HSV-2 in vitro and in vivo, with mechanisms distinct from acyclovir and involving interference with host genes and signaling pathways. WDL treatment improves survival, attenuates inflammatory symptoms, and reduces viral titres in infected mice.
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2023)
Article
Virology
Katrina A. Gianopulos, Tri Komala Sari, Darin J. Weed, Suzanne M. Pritchard, Anthony Nicola
Summary: Low endosomal pH facilitates herpesvirus entry in a cell-specific manner. The gC protein of HSV-1 plays a critical role in low-pH entry pathway by regulating the conformational changes of viral envelope glycoprotein B (gB). This study reveals that low pH triggers conformational changes in gC itself, and a specific gC epitope is important for low-pH entry inhibition.
JOURNAL OF VIROLOGY
(2022)
Article
Virology
Qing Fan, Richard Longnecker, Sarah A. Connolly
Summary: The viral fusion protein glycoprotein B (gB) is essential for virus entry in all herpesviruses. By introducing structure-based mutations, researchers identified mutations that counteracted a slow-entry phenotype in the virus and found that these mutations were located in different regions of the gB protein.
JOURNAL OF VIROLOGY
(2021)
Article
Virology
Sachith Polpitiya Arachchige, Wyatt Henke, Maria Kalamvoki, Edward B. Stephens
Summary: The study demonstrates that the gD glycoprotein of HSV-1 restricts HIV-1 infectivity, with its cytoplasmic tail domain playing a significant role in restriction activity, while the transmembrane domain of gB influences its incorporation into HIV-1 particles. Swapping domains between gD and gB shed light on the importance of gD cell surface expression and its interaction with HIV-1 particles.
JOURNAL OF VIROLOGY
(2021)
Article
Medicine, Research & Experimental
Sita Awasthi, James J. Knox, Angela Desmond, Mohamad-Gabriel Alameh, Brian T. Gaudette, John M. Lubinski, Alexis Naughton, Lauren M. Hook, Kevin P. Egan, Ying K. Tam, Norbert Pardi, David Allman, Eline T. Luning Prak, Michael P. Cancro, Drew Weissman, Gary H. Cohen, Harvey M. Friedman
Summary: Nucleoside-modified mRNA vaccines show more durable protection in animal models compared to protein vaccines, with higher neutralizing antibody titers and robust B cell immune memory. The correlation between high neutralizing titers and B cell immune memory likely explains the more lasting protection provided by the mRNA vaccine.
JOURNAL OF CLINICAL INVESTIGATION
(2021)
Article
Immunology
Kening Wang, Lesia Dropulic, Joel Bozekowski, Harlan L. Pietz, Sinthujan Jegaskanda, Kennichi Dowdell, Joshua S. Vogel, Doreen Garabedian, Makinna Oestreich, Hanh Nguyen, Mir A. Ali, Keith Lumbard, Sally Hunsberger, Jack Reifert, Winston A. Haynes, Jaymie R. Sawyer, John C. Shon, Patrick S. Daugherty, Jeffrey Cohen
Summary: The HSV-2 replication-defective vaccine HSV529 induces serum neutralizing antibody responses in most recipients. Vaccine recipients developed antibodies to epitopes of several viral proteins, although fewer epitopes were detected compared to naturally infected individuals. The vaccine also induces antibodies that mediate HSV-2-specific natural killer cell activation, which can reduce HSV genital infection by transporting potent serum antibodies to the genital tract.
JOURNAL OF INFECTIOUS DISEASES
(2021)
Review
Medical Laboratory Technology
Sita Awasthi, Harvey M. Friedman
Summary: The rapid development of two nucleoside-modified mRNA vaccines has revolutionized the field of COVID-19 vaccines with their safety and high effectiveness. The mRNA technology offers advantages such as accelerated immunogen discovery, robust immune response induction, and rapid manufacturing scale-up. Developing a genital herpes vaccine has been a longstanding public health priority, and the advent of mRNA technology holds promise in changing the narrative. The development of nucleoside-modified mRNA-lipid nanoparticle vaccines for genital herpes shows great potential in inducing higher levels of neutralizing antibodies and more durable immune responses compared to protein-adjuvanted vaccines.
TRANSLATIONAL RESEARCH
(2022)
Article
Allergy
Akimasa Adachi, Tetsuya Honda, Teruki Dainichi, Gyohei Egawa, Yosuke Yamamoto, Takashi Nomura, Saeko Nakajima, Atsushi Otsuka, Masamitsu Maekawa, Nariyasu Mano, Naoto Koyanagi, Yasushi Kawaguchi, Toshiaki Ohteki, Takashi Nagasawa, Koichi Ikuta, Akihiko Kitoh, Kenji Kabashima
Summary: The study revealed that a single bout of prolonged high-intensity exercise can either impair or enhance the clearance of HSV-2 infection in mice, depending on the timing of exercise post infection. This effect is linked to the temporary decrease or increase in blood-circulating plasmacytoid dendritic cells, regulated by exercise-induced glucocorticoids.
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
(2021)
Article
Pharmacology & Pharmacy
Mahmoud Kandeel, Mizuki Yamamoto, Byoung Kwon Park, Abdulla Al-Taher, Aya Watanabe, Jin Gohda, Yasushi Kawaguchi, Kentaro Oh-hashi, Hyung-Joo Kwon, Jun-ichiro Inoue
Summary: A highly potent set of peptides was designed to prevent MERS-CoV fusion through competition with HR2 at its HR1 binding site. These peptides demonstrated strong inhibition of MERS-CoV cell-cell fusion and plaque formation, with no cytotoxic effects at high concentrations, suggesting their potential as promising antiviral agents.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Yuriko Tachida, Saori Miura, Yui Muto, Hiroyuki Takuwa, Naruhiko Sahara, Akihiro Shindo, Yukio Matsuba, Takashi Saito, Naoyuki Taniguchi, Yasushi Kawaguchi, Hidekazu Tomimoto, Takaomi Saido, Shinobu Kitazume
Summary: The deposition of amyloid P in blood vessels of the brain is commonly observed in Alzheimer's disease patients. However, it is difficult to examine the contribution of cerebral amyloid angiopathy (CAA) to the pathogenesis of Alzheimer's disease in mouse models. Based on biochemical analyses, the researchers hypothesized that endothelial APP expression would be lower in rodents and subsequently generated mice that express human WT APP in endothelial cells. The mice showed increased levels of serum A beta and soluble APP, indicating the crucial role of endothelial APP in blood A beta levels. Crossing these mice with a specific APP knock-in mouse model led to an expanded CAA pathology.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Virology
Fumio Maeda, Akihisa Kato, Kosuke Takeshima, Misato Shibazaki, Ryota Sato, Takuma Shibata, Kensuke Miyake, Hiroko Kozuka-Hata, Masaaki Oyama, Eigo Shimizu, Seiya Imoto, Satoru Miyano, Shungo Adachi, Tohru Natsume, Koh Takeuchi, Yuhei Maruzuru, Naoto Koyanagi, Arii Jun, Kawaguchi Yasushi
Summary: This study developed a screening system to identify cellular proteins involved in the nuclear egress of HSV-1. Through this system, the researchers discovered that the cellular orphan transporter SLC35E1 plays a critical role in HSV-1 de-envelopment.
JOURNAL OF VIROLOGY
(2022)
Article
Microbiology
Mizuki Yamamoto, Jin Gohda, Ayako Kobayashi, Keiko Tomita, Youko Hirayama, Naohiko Koshikawa, Motoharu Seiki, Kentaro Semba, Tetsu Akiyama, Yasushi Kawaguchi, Jun-ichiro Inoue
Summary: The study found that the cell surface entry pathway of SARS-CoV-2 includes a metalloproteinase-mediated pathway in addition to the TMPRSS2 and endosomal pathways. The metalloproteinase-mediated pathway requires prior cleavage of the spike protein into two domains, a condition met by SARS-CoV-2 but not by the related human coronavirus SARS-CoV. Additionally, inhibiting metalloproteins was crucial in preventing cell death, which may lead to organ damage. The findings provide new insights into the unique pathogenesis of COVID-19 and are relevant to the development of effective therapies.
Article
Virology
Kosuke Takeshima, Yuhei Maruzuru, Naoto Koyanagi, Akihisa Kato, Yasushi Kawaguchi
Summary: This study demonstrates the redundant functions of lamin A/C and LBR in regulating HSV-1 gene expression and DNA replication through the dynamics of nuclear architecture. It also reveals the involvement of LBR in the regulation of viral and cellular features during HSV-1 infection.
JOURNAL OF VIROLOGY
(2022)
Article
Immunology
Ayano Fukui, Yuhei Maruzuru, Kosuke Takeshima, Naoto Koyanagi, Akihisa Kato, Yasushi Kawaguchi
Summary: In this study, a system was established to measure the frequency of cell-cell fusion induced by wild-type herpes simplex virus (HSV) strains. It was found that the HSV-1 envelope glycoprotein B and its N-glycosylation at asparagine at position 141 were necessary for efficient cell-cell fusion. This study provides insight into the connection between wild-type HSV-1-induced cell-cell fusion and viral pathogenesis in vivo.
MICROBIOLOGY AND IMMUNOLOGY
(2023)
Article
Multidisciplinary Sciences
Hideki Ogura, Jin Gohda, Xiuyuan Lu, Mizuki Yamamoto, Yoshio Takesue, Aoi Son, Sadayuki Doi, Kazuyuki Matsushita, Fumitaka Isobe, Yoshihiro Fukuda, Tai-Ping Huang, Takamasa Ueno, Naomi Mambo, Hiromoto Murakami, Yasushi Kawaguchi, Jun-ichiro Inoue, Kunihiro Shirai, Sho Yamasaki, Jun-Ichi Hirata, Satoshi Ishido
Summary: This study explores the characteristics of virus-specific CTLs related to the severity of COVID-19. Through the establishment of a cellular screening method, the researchers found that the SARS-CoV-2 M protein is a frequent target of CD8(+) T cells, and identified M198-206 as an immunoprevalent epitope in convalescent COVID-19 patients. Further single cell RNA sequencing revealed an exhausted phenotype with less differentiated status in cells from the severe group compared to cells from the moderate group.
NATURE COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Kazuto Takahashi, Kenji Kanekiyo, Kanoko Sakuda, Yui Muto, Masahiro Iguchi, Nozomu Matsuda, Yuko Hashimoto, Kazuaki Kanai, Haruko Ogawa, Hajime Hirase, Akiyoshi Kakita, Norihisa Bizen, Hirohide Takebayashi, Yasushi Kawaguchi, Miwa Uzuki, Shinobu Kitazume
Summary: Astrocytes, specifically those expressing HNK-1-O-Man(+) PTPRZ, play a crucial role in demyelination-associated conditions such as multiple sclerosis. This unique glycosylation pattern of PTPRZ is responsible for the pattern of reactive astrocytes in damaged brain areas. Understanding the role of HNK-1-O-Man(+) PTPRZ in astrocytes can provide insights into the development and progression of brain diseases.
JOURNAL OF NEUROCHEMISTRY
(2023)
Article
Cell Biology
Yoshihiko Kuchitsu, Kojiro Mukai, Rei Uematsu, Yuki Takaada, Ayumi Shinojima, Ruri Shindo, Tsumugi Shoji, Shiori Hamano, Emari Ogawa, Ryota Sato, Kensuke Miyake, Akihisa Kato, Yasushi Kawaguchi, Masahiko Nishitani-Isa, Kazushi Izawa, Ryuta Nishikomori, Takahiro Yasumi, Takehiro Suzuki, Naoshi Dohmae, Takefumi Uemura, Glen N. N. Barber, Hiroyuki Arai, Satoshi Waguri, Tomohiko Taguchi
Summary: Stimulator of interferon genes (STING) is crucial for the immune response against DNA pathogens, and its degradation is mediated by endosomal sorting complexes required for transport (ESCRT) proteins. STING-positive vesicles derived from recycling endosomes are encapsulated into lysosomes after STING ubiquitination. This study provides insights into the regulation of STING and prevents hyperactivation of the immune response.
NATURE CELL BIOLOGY
(2023)
Article
Virology
Ahmed H. E. Hassan, Selwan M. M. El-Sayed, Mizuki Yamamoto, Jin Gohda, Takehisa Matsumoto, Mikako Shirouzu, Jun-ichiro Inoue, Yasushi Kawaguchi, Reem M. A. Mansour, Abtin Anvari, Abdelbasset A. A. Farahat
Summary: Coronaviruses and other viruses rely on host proteases for cell entry. Targeting the conserved host-based entry mechanism instead of viral proteins may be advantageous. Compound 14 is identified as a potential starting point for developing viral entry inhibitors.
Article
Immunology
Joseph K. Sampson Duncan, Danyang Xu, Maria Licursi, Michael A. Joyce, Holly A. Saffran, Kaiwen Liu, Jin Gohda, D. Lorne Tyrrell, Yasushi Kawaguchi, Kensuke Hirasawa
Summary: Interferon regulatory factors (IRFs) play important roles in regulating the antiviral response against human coronaviruses. Activation of IRF1, IRF3, and IRF7 is observed during infection with different strains of coronaviruses. Knockdown and overexpression experiments demonstrate that IRF1 and IRF3 have antiviral properties against OC43, while IRF3 and IRF7 are effective in restricting 229E infection. Our study highlights the potential of IRFs as antiviral regulators against human coronavirus infection.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Microbiology
Yujin Inayoshi, Shiori Oguro, Erika Tanahashi, Zhifeng Lin, Yasushi Kawaguchi, Toshiaki Kodama, Chihiro Sasakawa
Summary: A reverse genetics system was established in this study to clone and manipulate the avian infectious bronchitis virus (IBV) genome, providing a useful platform for analyzing the pathogenesis of IBV and developing new vaccines.
CURRENT RESEARCH IN MICROBIAL SCIENCES
(2022)
Article
Virology
Jun Arii, Kosuke Takeshima, Yuhei Maruzuru, Naoto Koyanagi, Yoshitaka Nakayama, Akihisa Kato, Yasuko Mori, Yasushi Kawaguchi
Summary: This study identifies an arginine cluster in the disordered domain of UL34 that interacts with ALIX and recruits ESCRT-III machinery for primary envelopment. The study highlights the importance of the disordered domain of UL34 in herpesvirus infections.
JOURNAL OF VIROLOGY
(2022)
Article
Virology
Mizuki Watanabe, Jun Arii, Kosuke Takeshima, Ayano Fukui, Masayuki Shimojima, Hiroko Kozuka-Hata, Masaaki Oyama, Takeharu Minamitani, Teruhito Yasui, Yuji Kubota, Mutsuhiro Takekawa, Isao Kosugi, Yuhei Maruzuru, Naoto Koyanagi, Akihisa Kato, Yasuko Mori, Yasushi Kawaguchi
Summary: The host cell protein prohibitin-1 contributes to HSV-1 cell-to-cell spread via the MAPK/ERK pathway, a mechanism that is conserved in representative members of every herpesvirus subfamily.
JOURNAL OF VIROLOGY
(2021)