Article
Immunology
Andy Dernstedt, Jana Leidig, Anna Holm, Priscilla F. Kerkman, Jenny Mjosberg, Clas Ahlm, Johan Henriksson, Magnus Hultdin, Mattias N. E. Forsell
Summary: The study found that B cells downregulate DAF upon engagement with BCR, and T cell-dependent stimuli preferentially activate DAF(lo) B cells. DAF(lo) B cells in GC are more susceptible to complement-dependent phagocytosis, while DAF(hi) GC B cells show increased expression of the plasma cell marker Blimp-1.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Biochemical Research Methods
Maria G. Detsika, K. Goudevenou, A. M. Geurts, H. Gakiopoulou, E. Grapsa, Elias A. Lianos
Summary: A novel DAF deficient rat model was successfully generated using CRISPR/Cas9 technology. The model exhibited complete absence of DAF in renal and extra-renal tissues and showed significant proteinuria and increased C3b deposition following complement-dependent injury experiments. This model provides a useful tool for studying complement-dependent kidney injury.
TRANSGENIC RESEARCH
(2021)
Article
Oncology
Harley Robinson, Jayde E. Ruelcke, Amanda Lewis, Charles S. Bond, Archa H. Fox, Vandhana Bharti, Shivangi Wani, Nicole Cloonan, Andrew Lai, David Margolin, Li Li, Carlos Salomon, Renee S. Richards, Aine Farrell, Robert A. Gardiner, Robert G. Parton, Alexandre S. Cristino, Michelle M. Hill
Summary: The study reveals a novel mechanism in which non-caveolar CAV1 drives the loading of metastasis-promoting microRNAs into exosomes by regulating hnRNPK localization, with this process being modulated by the lipid environment of membranes. Knockdown of hnRNPK in prostate cancer cells impairs the ability of extracellular vesicles to induce osteoclastogenesis, indicating a potential role in bone metastasis. Additionally, elevated levels of hnRNPK in biofluid extracellular vesicles are associated with metastatic prostate and colorectal cancer.
CLINICAL AND TRANSLATIONAL MEDICINE
(2021)
Review
Materials Science, Biomaterials
Jon Ander Nieto-Garai, Francesc-Xabier Contreras, Aroa Arboleya, Maier Lorizate
Summary: Viral entry is a sequential process involving the attachment of the virus to the host cell and the introduction of its genetic material. Both cellular and viral lipids play crucial roles in viral entry. This article summarizes the specific roles of lipids and lipid membrane nanostructures in viral attachment, fusion, and immune evasion, and discusses the limitations of commonly used techniques for studying protein-lipid interactions, as well as emerging tools in this field.
Article
Biochemistry & Molecular Biology
Kyoung Ok Jang, Youn Woo Lee, Hangeun Kim, Dae Kyun Chung
Summary: Staphylococcus aureus proliferates internally in host cells through internalization and evasion of the host immune system, eventually being released through induction of cell death. Infected host cells increase specific surface protein expression to inform immune cells of the infection.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Physics, Multidisciplinary
Long Li, Jinglei Hu, Bartosz Rozycki, Xiaohuan Wang, Helong Wu, Fan Song
Summary: Studies have found that lipid rafts can promote the formation of a ring of LFA1-ICAM1 complexes around a central domain of TCR-MHCp complexes in T cell adhesion, even in the absence of active transport of TCR molecules towards the center of the contact zone. In the presence of active TCR transport, lipid rafts can accelerate the formation of this monocentric pattern. Additionally, it has been shown that lipid rafts have a strong stabilizing effect on the monocentric pattern after removal of the active TCR transport.
NEW JOURNAL OF PHYSICS
(2021)
Review
Biochemistry & Molecular Biology
Long Li, Jing Ji, Fan Song, Jinglei Hu
Summary: Understanding the intercellular receptor-ligand binding is crucial for various physiological and pathological processes, as well as drug design. This review focuses on the regulatory effects of protein-membrane interactions on intercellular receptor-ligand binding, including membrane fluctuations, membrane curvature, glycocalyx, and lipid raft. The article discusses the biomedical significance and potential research directions to advance the field and emphasizes the importance of understanding the coupling effects of these factors in pharmaceutical development.
JOURNAL OF MOLECULAR BIOLOGY
(2023)
Review
Medicine, Research & Experimental
Siyu Zhang, Yuxin Tong
Summary: In this review, we summarize the structure, post-translational modifications, and cellular pathways regulated by SoHo family proteins, with a focus on ArgBP2. We also discuss the significance of SoHo family proteins in cellular distribution, extracellular matrix microenvironments, and drug discovery.
BIOMEDICINE & PHARMACOTHERAPY
(2022)
Review
Chemistry, Physical
Claudia Steinem, Michael Meinecke
Summary: Cellular membranes are not flat structures, and their shapes have evolved for specific physiological reasons. In membrane trafficking, dynamic reshaping of cellular membranes is apparent, as seen in clathrin-mediated endocytosis where the plasma membrane undergoes heavy deformation.
Review
Biochemistry & Molecular Biology
Wen-Yi Low, Shu-Sin Chng
Summary: The outer membrane of Gram-negative bacteria has unique lipid asymmetry that acts as a barrier against toxic molecules, including antibiotics. The OmpC-Mla system plays a central role in maintaining this lipid asymmetry by retrograde transport of phospholipids. Recent advances in structural elucidations and biochemical reconstitutions have provided detailed insights into the molecular mechanism of this lipid trafficking process.
CURRENT OPINION IN CHEMICAL BIOLOGY
(2021)
Article
Cell Biology
Kyle A. Lyman, Ye Han, Chengwen Zhou, Isabelle Renteria, Gai-Linn Besing, Jonathan E. Kurz, Dane M. Chetkovich
Summary: Research shows that cAMP signaling can impact behavior through the regulation of HCN channels, and chronically elevated cAMP can alleviate cognitive deficits induced by chronic stress by affecting the surface trafficking of HCN channels.
SCIENCE TRANSLATIONAL MEDICINE
(2021)
Article
Biophysics
Alex L. Lai, Jack H. Freed
Summary: This study investigates the interaction between the fusion peptide of coronaviruses and host cell membranes, revealing the crucial role of negatively charged residues and the involvement of calcium ions. The findings provide insights into the membrane fusion process and may contribute to the development of antiviral strategies targeting this mechanism.
BIOPHYSICAL JOURNAL
(2022)
Review
Neurosciences
Zack Blumenfeld, Kallol Bera, Eero Castren, Henry A. Lester
Summary: This article summarizes the therapeutic actions of antidepressants when they encounter their targets inside cells or intracellular organelles. It discusses the chemical, pharmacokinetic, and pharmacodynamic principles underlying drug movements into subcellular compartments. The article highlights the importance of charge and hydrophobicity/lipid solubility in drug membrane permeation and provides data and calculations for various antidepressants regarding their structures, charge properties, and distribution volume. It also reviews the pharmacokinetics of ketamine, ketamine metabolites, and other rapidly acting antidepressants, as well as the mechanisms involving intracellular targets.
NEUROPSYCHOPHARMACOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Ricardo Capone, Ajit Tiwari, Arina Hadziselimovic, Yelena Peskova, James M. Hutchison, Charles R. Sanders, Anne K. Kenworthy
Summary: Research has shown that C99 is predominantly located in disordered domains in cell-derived giant plasma membrane vesicles, with little association with ordered domains.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Oncology
Gregory L. Beatty, Jasmine S. Smith, Ran Reshef, Kunal P. Patel, Theresa A. Colligon, Barbara A. Vance, Noelle V. Frey, F. Brad Johnson, David L. Porter, Robert H. Vonderheide
CLINICAL CANCER RESEARCH
(2009)
Article
Cell Biology
Rebecca A. Bozym, Kunal Patel, Carl White, King-Ho Cheung, Jeffrey M. Bergelson, Stefanie A. Morosky, Carolyn B. Coyne
MOLECULAR BIOLOGY OF THE CELL
(2011)
Editorial Material
Biochemistry & Molecular Biology
Kunal P. Patel, Jeffrey M. Bergelson
Article
Multidisciplinary Sciences
Omar Khan, Josephine R. Giles, Sierra McDonald, Sasikanth Manne, Shin Foong Ngiow, Kunal P. Patel, Michael T. Werner, Alexander C. Huang, Katherine A. Alexander, Jennifer E. Wu, John Attanasio, Patrick Yan, Sangeeth M. George, Bertram Bengsch, Ryan P. Staupe, Greg Donahue, Wei Xu, Ravi K. Amaravadi, Xiaowei Xu, Giorgos C. Karakousis, Tara C. Mitchell, Lynn M. Schuchter, Jonathan Kaye, Shelley L. Berger, E. John Wherry
Article
Critical Care Medicine
William T. Atchley, Carolina Alvarez, Shruti Saxena-Beem, Todd A. Schwartz, Rumey C. Ishizawar, Kunal P. Patel, M. Patricia Rivera
Summary: In this retrospective case-control study, it was found that immune checkpoint inhibitor-related pneumonitis (ICI-P) was more common and severe in lung cancer patients than previously reported, and was independently associated with several chronic pulmonary diseases.
Letter
Critical Care Medicine
William T. Atchley, Little Rock, Carolina Alvarez, Shruti Saxena-Beem, Todd A. Schwartz, Rumey C. Ishizawar, Kunal P. Patel, M. Patricia Rivera
Article
Microbiology
ME Sebert, KP Patel, M Plotnick, JN Weiser
JOURNAL OF BACTERIOLOGY
(2005)
Article
Immunology
JD Schilling, SM Martin, DA Hunstad, KP Patel, MA Mulvey, SS Justice, RG Lorenz, SJ Hultgren
INFECTION AND IMMUNITY
(2003)
Review
Biotechnology & Applied Microbiology
KP Patel, RH Vonderheide