Article
Pharmacology & Pharmacy
Sergio E. Rodriguez, David W. Hawman, Teresa E. Sorvillo, T. Justin O'Neal, Brian H. Bird, Luis L. Rodriguez, Eric Bergeron, Stuart T. Nichol, Joel M. Montgomery, Christina F. Spiropoulou, Jessica R. Spengler
Summary: This article reviews the progress in immunobiology research of Crimean-Congo hemorrhagic fever virus (CCHFV), including the relationship between different outcomes and disease severity, and provides recommendations for future research.
ANTIVIRAL RESEARCH
(2022)
Review
Medicine, General & Internal
Aykut Ozdarendeli
Summary: Crimean-Congo hemorrhagic fever virus (CCHFV) is a highly infectious virus transmitted to humans through tick bites or contact with infected animal blood, causing severe symptoms and a high mortality rate. The lack of effective medical countermeasures and its potential for global spread have made CCHFV a high-priority pathogen. Recent advances in biotechnology and suitable animal models have accelerated the development of CCHFV vaccines.
Letter
Immunology
Mohammad M. Obaidat, James C. Graziano, Maria Morales-Betoulle, Shelley M. Brown, Cheng-Feng Chiang, John D. Klena
Summary: The epidemiology of Rift Valley fever virus (RVFV) and Crimean-Congo hemorrhagic fever virus (CCHFV) in Jordan is unknown. A study found 3% of tested dairy cattle, sheep, and goats were RVFV seropositive and 14% were CCHFV seropositive. Ongoing surveillance is necessary to evaluate the risk to humans and protect public health.
EMERGING INFECTIOUS DISEASES
(2021)
Review
Virology
Mohammad Fereidouni, Dmitry A. Apanaskevich, David B. Pecor, Natalia Yu. Pshenichnaya, Gulzhan N. Abuova, Farida H. Tishkova, Yekaterina Bumburidi, Xiankun Zeng, Jens H. Kuhn, Maryam Keshtkar-Jahromi
Summary: This study summarizes the status of Crimean-Congo hemorrhagic fever (CCHF) in Central, Eastern, and South-eastern Asia. The risk and burden of CCHF were assessed based on case reports, antibody prevalence, and vector ticks isolation. The majority of cases were reported in Central Asia, while only China reported cases in Eastern Asia. No cases were reported in South-eastern Asia. Countries were classified into different levels based on evidence of CCHF, guiding the strengthening or establishment of CCHF surveillance systems.
Article
Infectious Diseases
Nikolay Tsapko, Anna S. Volynkina, Anna Yu Evchenko, Yana Lisitskaya, Ludmila Shaposhnikova
Summary: Crimean-Congo hemorrhagic fever (CCHF) plays a central role in tick-borne infections in southern Russia, with an expansion of the disease across regions. Ticks are the main vectors and reservoirs of the CCHF virus (CCHFV), which has been detected in 33 tick species. The study found the virus in various tick species, with H. marginatum being the main vector.
TICKS AND TICK-BORNE DISEASES
(2022)
Article
Parasitology
Jyoti S. Kumar, Manmohan Parida, Anita M. Shete, Triparna Majumdar, Savita Patil, Pragya D. Yadav, Paban Kumar Dash
Summary: A rapid and sensitive RT-LAMP assay for detection of CCHFV has been developed in this study, which can be completed in a short time, providing crucial information for outbreak control and clinical management.
Article
Immunology
Johan Espunyes, Oscar Cabezon, Lola Pailler-Garcia, Andrea Dias-Alves, Lourdes Lobato-Bailon, Ignasi Marco, Maria P. Ribas, Pedro E. Encinosa-Guzman, Marta Valldeperes, Sebastian Napp
Summary: A serosurvey in Catalonia, northeastern Spain, found high seroprevalence of Crimean-Congo hemorrhagic fever virus antibodies in southern Catalonia, indicating potential presence of competent virus vectors in the region.
EMERGING INFECTIOUS DISEASES
(2021)
Article
Virology
Serdal Arslan, Burcu Bayyurt, Aynur Engin, Mehmet Bakir
Summary: The study identified significant changes in miRNA expression in patients with Crimean Congo hemorrhagic fever, with some miRNAs showing differential expression in acute and severe cases. These miRNAs are associated with antiviral and proinflammatory pathways, suggesting potential therapeutic targets for the acute phase of the disease.
JOURNAL OF MEDICAL VIROLOGY
(2021)
Article
Virology
Feyza Izci, Zeynep Ture, Pinar Sagiroglu, Sahin Temel, Orhan Yildiz
Summary: This study reports a case of severe CCHF with Clostridium perfringens bacteremia in a female patient, who presented with symptoms such as fever, nausea, vomiting, and abdominal pain. The diagnosis was confirmed using real-time polymerase chain reaction, and successful treatment was achieved through a multidisciplinary approach.
JOURNAL OF MEDICAL VIROLOGY
(2021)
Article
Virology
Nilgun Altin, Fatma Aybala Altay, Murat Albayrak, Seyda Ozdemir Sahingoz, Irfan Sencan
Summary: This study evaluates the relationship between TAFI levels and bleeding as well as prognosis in CCHF patients. The results show that although TAFI levels are lower in CCHF patients compared to healthy controls, it does not seem to be a major factor in prognosis.
JOURNAL OF MEDICAL VIROLOGY
(2023)
Article
Immunology
Anabel Negredo, Rafael Sanchez-Arroyo, Francisco Diez-Fuertes, Fernando de Ory, Marco Antonio Budino, Ana Vazquez, Angeles Garcinuno, Lourdes Hernandez, Cesar de la Hoz Gonzalez, Almudena Gutierrez-Arroyo, Carmen Grande, Paz Sanchez-Seco
Summary: In August 2018, a fatal autochthonous case of Crimean-Congo hemorrhagic fever was confirmed in western Spain, with a new virus circulating as the genotype differs from a previous case in 2016. Practitioners must remain alert to the possibility of new cases.
EMERGING INFECTIOUS DISEASES
(2021)
Letter
Immunology
Ana Negredo, Maria Sanchez-Ledesma, Francisco Llorente, Mayte Perez-Olmeda, Moncef Belhassen-Garcia, David Gonzalez-Calle, Maria Paz Sanchez-Seco, Miguel Angel Jimenez-Clavero
Summary: The earliest cases of emerging diseases are often difficult to recognize.
EMERGING INFECTIOUS DISEASES
(2021)
Article
Infectious Diseases
Shohra Qaderi, Masoud Mardani, Asghar Shah, Jaffer Shah, Narges Bazgir, Jalal Sayad, Ehsan Ghandchi, Majid Samsami, Javad Zebarjadi Bagherpour
Summary: The prevalence of CCHF is increasing in Afghanistan, with a majority of cases seeking medical care during the late phase of infection, which could negatively impact treatment efficacy. Impaired Alanine aminotransferase (ALT), hemorrhagic manifestations (including epistaxis), and impaired consciousness were factors associated with a higher mortality rate, potentially providing clues for early effective interventions.
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
(2021)
Article
Virology
Zeynep Ture, Gamze Kalin-Unuvar
Summary: Crimean-Congo hemorrhagic fever (CCHF) is a common tick-borne hemorrhagic infection. Apart from fever and hemorrhage, CCHF can present with different clinical manifestations. This study reports a rare case of a female patient with pulmonary embolism associated with severe CCHF, who was successfully treated with supportive and anticoagulation therapy.
JOURNAL OF MEDICAL VIROLOGY
(2021)
Article
Multidisciplinary Sciences
Anoop T. Ambikan, Nazif Elaldi, Sara Svensson- Akusjaervi, Binnur Bagci, Ayse Nur Pektas, Roger Hewson, Gokhan Bagci, Mehmet Arasli, Sofia Appelberg, Adil Mardinoglu, Vikas Sood, Akos Vegvari, Rui Benfeitas, Soham Gupta, Ilhan Cetin, Ali Mirazimi, Ujjwal Neogi
Summary: This study analyzed the trajectory of host response and metabolic changes following CCHFV infection, revealing that antiviral immune responses are regulated by genes and signaling pathways. The reorganization of metabolism during the acute phase contributes to pathogenicity, while downregulated metabolic processes during the convalescent phase may lead to metabolic rewiring.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Biochemical Research Methods
Katrin Zwirglmaier, Maria Weyh, Christian Krueger, Rosina Ehmann, Katharina Mueller, Roman Woelfel, Kilian Stoecker
Summary: In the current COVID-19 pandemic, a novel nucleic acid-based testing format called pulse controlled amplification was introduced, which is capable of detecting SARS-CoV-2 directly from multiple swab samples within 25 minutes without the need for RNA extraction, with a sensitivity of 100% for samples with high viral loads. This new approach may lead to rapid and sensitive point-of-care testing.
JOURNAL OF VIROLOGICAL METHODS
(2021)
Article
Multidisciplinary Sciences
Alexey Stukalov, Virginie Girault, Vincent Grass, Ozge Karayel, Valter Bergant, Christian Urban, Darya A. Haas, Yiqi Huang, Lila Oubraham, Anqi Wang, M. Sabri Hamad, Antonio Piras, Fynn M. Hansen, Maria C. Tanzer, Igor Paron, Luca Zinzula, Thomas Engleitner, Maria Reinecke, Teresa M. Lavacca, Rosina Ehmann, Roman Woelfel, Joerg Jores, Bernhard Kuster, Ulrike Protzer, Roland Rad, John Ziebuhr, Volker Thiel, Pietro Scaturro, Matthias Mann, Andreas Pichlmair
Summary: The study reports a concurrent multi-omics analysis of SARS-CoV-2 and SARS-CoV, revealing both distinct and common molecular mechanisms between these closely related coronaviruses, as well as their impact on the host cell proteome. The results of this study may provide insights into the design of virus- and host-directed therapies for these coronaviruses.
Article
Immunology
Antonia Sophia Peter, Edith Roth, Sebastian R. Schulz, Kirsten Fraedrich, Tobit Steinmetz, Dominik Damm, Manuela Hauke, Elie Richel, Sandra Mueller-Schmucker, Katharina Habenicht, Valentina Eberlein, Leila Issmail, Nadja Uhlig, Simon Dolles, Eva Gruner, David Peterhoff, Sandra Ciesek, Markus Hoffmann, Stefan Pohlmann, Paul F. McKay, Robin J. Shattock, Roman Wolfel, Eileen Socher, Ralf Wagner, Jutta Eichler, Heinrich Sticht, Wolfgang Schuh, Frank Neipel, Armin Ensser, Dirk Mielenz, Matthias Tenbusch, Thomas H. Winkler, Thomas Grunwald, Klaus Uberla, Hans-Martin Jack
Summary: TRIANNI mice were used to generate 29 hybridoma antibodies that reacted with the SARS-CoV-2 spike protein, and identified two clusters of neutralizing antibodies targeting different regions of the spike protein. These neutralizing antibodies significantly reduced viral spread and protected mice from SARS-CoV-2-induced weight loss, showing potential for therapy and prophylaxis of COVID-19.
EUROPEAN JOURNAL OF IMMUNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Laura Olbrich, Noemi Castelletti, Yannik Schaelte, Merce Gari, Peter Puetz, Abhishek Bakuli, Michael Pritsch, Inge Kroidl, Elmar Saathoff, Jessica Michelle Guggenbuehl Noller, Volker Fingerle, Ronan Le Gleut, Leonard Gilberg, Isabel Brand, Philine Falk, Alisa Markgraf, Flora Deak, Friedrich Riess, Max Diefenbach, Tabea Eser, Franz Weinauer, Silke Martin, Ernst-Markus Quenzel, Marc Becker, Juergen Durner, Philipp Girl, Katharina Mueller, Katja Radon, Christiane Fuchs, Roman Woelfel, Jan Hasenauer, Michael Hoelscher, Andreas Wieser
Summary: This study compared different SARS-CoV-2 testing methods using a large sample size and found varying levels of sensitivity, specificity, and effectiveness. In true-positive samples, the titres of serological indicators decreased over time, particularly in the period of 30 to 90 days after testing positive for the virus.
JOURNAL OF GENERAL VIROLOGY
(2021)
Article
Biochemical Research Methods
Fee Zimmermann, Maria Urban, Christian Krueger, Mathias Walter, Roman Woelfel, Katrin Zwirglmaier
Summary: RT-qPCR assays are widely used for SARS-CoV-2 detection, but mismatches in primers can affect performance negatively. Forward primer mismatches have a more detrimental effect than reverse primer mismatches. The design of primers can lead to variations in ct values.
JOURNAL OF VIROLOGICAL METHODS
(2022)
Article
Environmental Sciences
Raquel Rubio-Acero, Jessica Beyerl, Muenchhoff Maximilian, Marc Sancho Roth, Noemi Castelletti, Ivana Paunovic, Katja Radon, Bernd Springer, Christian Nagel, Bernhard Boehm, Merle M. Boehmer, Alexander Graf, Helmut Blum, Stefan Krebs, Oliver T. Keppler, Andreas Osterman, Zohaib Nisar Khan, Michael Hoelscher, Andreas Wieser
Summary: Wastewater-based epidemiology is an increasingly proposed tool for monitoring the burden of SARS-CoV-2 in populations, predicting the dynamics of local outbreaks and the introduction of variants of concern.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Virology
Kerstin Puchinger, Noemi Castelletti, Raquel Rubio-Acero, Christof Geldmacher, Tabea M. Eser, Flora Deak, Ivana Paunovic, Abhishek Bakuli, Elmar Saathoff, Alexander von Meyer, Alisa Markgraf, Philine Falk, Jakob Reich, Friedrich Riess, Philipp Girl, Katharina Mueller, Katja Radon, Jessica Michelle Guggenbuehl Noller, Roman Woelfel, Michael Hoelscher, Inge Kroidl, Andreas Wieser, Laura Olbrich
Summary: This study investigated the dynamics of viral loads and antibodies in COVID-19 patients and found a correlation between viral loads and clinical progression.
Article
Genetics & Heredity
Katharina Mueller, Philipp Girl, Andreas Giebl, Stefanie Gruetzner, Markus Antwerpen, Elham Khatamzas, Roman Woelfel, Heiner von Buttlar
Summary: Studies have shown that variants of SARS-CoV-2 have significantly reduced sensitivity to neutralizing antibodies, with some even completely resistant to neutralization. Individual mutations in the spike protein are sufficient to allow the virus to evade the humoral immune response, and the effect of escape mutations appears to accumulate over time.
Article
Medicine, General & Internal
Philipp Girl, Katrin Zwirglmaier, Heiner von Buttlar, Roman Woelfel, Katharina Mueller
Summary: This study evaluated the performance of four commercially available tests for detecting SARS-CoV-2 neutralizing antibodies. The overall sensitivity and specificity varied among lateral flow assays, while surrogate ELISA tests showed higher sensitivity. All tests demonstrated the highest sensitivity when testing samples from fully vaccinated individuals.
FRONTIERS IN MEDICINE
(2022)
Article
Hematology
Qiuyue Ma, Roland Immler, Monika Pruenster, Markus Sellmayr, Chenyu Li, Albrecht von Brunn, Brigitte von Brunn, Rosina Ehmann, Roman Woelfel, Matteo Napoli, Qiubo Li, Paola Romagnani, Ralph Thomas Boettcher, Markus Sperandio, Hans-Joachim Anders, Stefanie Steiger
Summary: This study investigates the impact of uric acid on neutrophils and finds that hyperuricemia impairs neutrophil function. Partial reduction of uric acid levels using uricase can partially restore the neutrophil defects. The study reveals that uric acid affects neutrophil migration by regulating intracellular pH and cytoskeletal dynamics. These findings have important implications for understanding immunodeficiency in kidney disease and sterile inflammation.
Article
Virology
Philipp Girl, Sonja Mantel, Heiner von Buttlar, Roman Woelfel, Katharina Mueller
Summary: In December 2020, the World Health Organization introduced the first international standard for anti-SARS-CoV-2 immunoglobulin. This study compared three different commercial ELISA kits for the quantification of SARS-CoV-2 IgG antibodies and found that the agreement between quantitative ELISAs is variable and they cannot be used interchangeably despite calibration against a standard. The results highlight the importance of individual interpretation and the limitation of quantitative ELISAs in replacing neutralization tests.
Article
Infectious Diseases
Sebastian Noe, Sabine Zange, Michael Seilmaier, Markus H. Antwerpen, Thomas Fenzl, Jochen Schneider, Christoph D. Spinner, Joachim J. Bugert, Clemens-Martin Wendtner, Roman Woelfel
Summary: This article presents the first two cases of human monkeypox infection diagnosed in Germany. The importance of rapid diagnosis and public health response is emphasized, along with the need for differential diagnosis of rash, especially in patients with frequent sexual contacts.
Article
Virology
Maximilian Kittel, Romy Eichner, Sihem Aida, Anna Bode, Volker Ast, Anja Kessler, Michael Neumaier, Roman Woelfel, Verena Haselmann
Summary: This study revealed serious concerns regarding the reliability and standardization of anti-SARS-CoV-2 antibody assays. Only 64% of participating laboratories successfully passed the external quality assessment, highlighting the importance of accurate interpretation of test results.
Article
Microbiology
Flavia Dematheis, Mathias C. Walter, Daniel Lang, Markus Antwerpen, Holger C. Scholz, Marie-Theres Pfalzgraf, Enrico Mantel, Christin Hinz, Roman Wolfel, Sabine Zange
Summary: In this study, MALDI-TOF mass spectrometry was used to differentiate closely related species of Brucella. The results showed that both unsupervised and optimized supervised machine learning models achieved excellent predictive performances.
Article
Hematology
Hye Kyung Lee, Manuela A. Hoechstetter, Maike Buchner, Trang Thu Pham, Jin Won Huh, Katharina Mueller, Sabine Zange, Heiner von Buttlar, Philipp Girl, Roman Woelfel, Lisa Brandmeier, Lisa Pfeuffer, Priscilla A. Furth, Clemens -Martin Wendtner, Lothar Hennighausen
Summary: Patients with CLL treated with B-cell pathway inhibitors and anti-CD20 antibodies have low humoral response rates after SARS-CoV-2 vaccination. A study compared the transcriptional response of peripheral blood mononuclear cells with antibody and T-cell response rates in CLL/SLL patients who received heterologous BNT162b2/ChAdOx1 vaccination. The antibody response rate after two doses was 40%, which increased to 53% after the booster dose. The T-cell response rate after two doses was 80%, increasing to 93% after the booster.
Article
Biochemical Research Methods
Atif Khurshid Wani, Chirag Chopra, Daljeet Singh Dhanjal, Nahid Akhtar, Himanshu Singh, Poorvi Bhau, Anjuvan Singh, Varun Sharma, Rafael Silvio Bonilha Pinheiro, Juliana Heloisa Pine Americo-Pinheiro, Reena Singh
Summary: Zoonotic viral infections pose significant threats to global public health. Understanding the origins and dynamics of these infections is crucial for prevention and management of future outbreaks. Metagenomics is a powerful tool for studying the diversity of viral populations and potential zoonotic events.
JOURNAL OF VIROLOGICAL METHODS
(2024)
Article
Biochemical Research Methods
Michel V. F. Sucupira, Ana P. C. Argondizzo, Mariana Miguez, Anna E. de Araujo, Leila B. R. Silva, Marcelle B. Mello, Christiane F. S. Marques, Danielle Brito Cunha, Renata C. Bastos, Vanessa S. de Paula, Luciane A. Amado Leon
Summary: This study focuses on improving the purification, solubility, and protein expression levels of recombinant VP1-2A, a hepatitis A virus (HAV) structural protein with immunogenic activity. The results show that the modified approach successfully produces biologically active HAV VP1-2A, which can be used as a diagnostic tool for HAV infection.
JOURNAL OF VIROLOGICAL METHODS
(2024)
Article
Biochemical Research Methods
Arun K. Dhar, Roberto Cruz-Flores, Hung N. Mai, Janet Warg
Summary: A sensitive and robust TaqMan PCR method for detecting IHHNV in three commercially important shrimp species was developed and compared with other published methods. Multiple primer/probe sets, including qIH-Fw/qIH-Rv and 3144F/3232R, are recommended for the detection of IHHNV. These findings are valuable for large-scale screening of shrimp using a TaqMan real-time PCR assay.
JOURNAL OF VIROLOGICAL METHODS
(2024)
Article
Biochemical Research Methods
Kemal Mehinagic, Matthias Liniger, Maksym Samoilenko, Nick Soltermann, Markus Gerber, Nicolas Ruggli
Summary: A plasmid-based reporter assay has been developed for sensitive detection and titration of infectious African swine fever virus (ASFV). This novel method allows accurate measurement of ASFV titers and is applicable to ASFV of different genotypes, virulence, and sources.
JOURNAL OF VIROLOGICAL METHODS
(2024)
Article
Biochemical Research Methods
Jan Van Slambrouck, Charlotte Schoenaers, Lies Laenen, Xin Jin, Kurt Beuselinck, Ann Verdonck, Joost Wauters, Geert Molenberghs, Bart M. Vanaudenaerde, Robin Vos, Peter Mombaerts, Katrien Lagrou, Laurens J. Ceulemans
Summary: The ability to detect SARS-CoV-2 RNA or antigen in BAL fluid samples using point-of-care tests (POCTs) can reduce the risk of developing severe COVID-19 after lung transplantation.
JOURNAL OF VIROLOGICAL METHODS
(2024)