Article
Biochemical Research Methods
Junsheng Dong, Wai Ning Tiffany Tsui, Xue Leng, Jinping Fu, Molly Lohman, Joseph Anderson, Vaughn Hamill, Nanyan Lu, Elizabeth Poulsen Porter, Mark Gray, Tesfaalem Sebhatu, Susan Brown, Roman Pogranichniy, Heng Wang, Lance Noll, Jianfa Bai
Summary: Infectious respiratory disease is a common disease in dogs worldwide. A multiplex real-time PCR assay was developed in this study to accurately and rapidly identify the causative agents of canine infectious respiratory disease. The assay showed high diagnostic sensitivity and specificity.
JOURNAL OF MICROBIOLOGICAL METHODS
(2022)
Article
Clinical Neurology
Yanjun Si, Weijun He, Shuo Guo, Xiaohui Wang, Meng Tang, Binwu Ying, Minjin Wang
Summary: This study investigated the clinical utility and epidemiological characteristics of ME in southwestern China using a novel multiplex PCR detection method. The results showed that MME-18 had good consistency with conventional testing for ME diagnosis, aiding in early pathogen identification.
FRONTIERS IN NEUROLOGY
(2022)
Article
Chemistry, Analytical
Kerou Zhang, Alessandro Pinto, Lauren Yuxuan Cheng, Ping Song, Peng Dai, Michael Wang, Luis Rodriguez, Cailin Weller, David Yu Zhang
Summary: In this study, we developed an occlusion system that functions as an efficient fluorescence indicator in HiFi qPCR reactions, improving the detection sensitivity for early cancer diagnosis and drug-resistance mutation identification.
ANALYTICAL CHEMISTRY
(2022)
Article
Multidisciplinary Sciences
Yun Li, LuJie Zhao, Jingye Wang, Peipei Qi, Zhongfa Yang, Xiangyu Zou, Fujun Peng, Shengguang Li
Summary: The study aimed to establish a new method for rapid identification of common sepsis pathogens and evaluate its clinical application. A multiplex PCR assay was used to simultaneously amplify specific conserved regions of nine common pathogenic microorganisms. The assay showed high sensitivity and accuracy in detecting pathogens, making it useful for early initiation of effective antimicrobial treatment and routine clinical practice identification of sepsis pathogens.
Article
Veterinary Sciences
Juan Zhang, Wan Wang, Mujiao Yang, Jun Lin, Fei Xue, Yuanmao Zhu, Xin Yin
Summary: A one-step multiplex real-time PCR method was developed to detect viral pathogens causing bovine respiratory disease complex (BRDC). The method showed good specificity and sensitivity, and could detect multiple viruses simultaneously, providing technical support for rapid clinical diagnosis of BRDC-associated viruses.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Food Science & Technology
Christian Brenn, Ute Schroeder, Reinhold Hanel, Pedro Martinez Arbizu
Summary: The study presents a TaqManprobe based multiplex real-time PCR screening assay for identification of four commercially relevant crustacean species, showing high specificity and potential for rapid species identification.
Article
Multidisciplinary Sciences
In Bum Suh, Jaegyun Lim, Hyo Seon Kim, Guil Rhim, Heebum Kim, Hana Kim, Sae-Mi Lee, Hyun-Sang Park, Hyun Ju Song, MyungKook Hong, Gyung Sook Shin, Moon Jung Kim
Summary: This research introduces the development of two novel multiplex real-time RT-PCR kits for the detection of SARS-CoV-2, which demonstrated high precision and compatibility with various sample types. The clinical performance of these kits showed a high level of agreement with a standard confirmation assay, making them suitable for effective management and control of COVID-19.
Article
Chemistry, Analytical
Sishuo Song, Fan Ji, Qingdong Zeng, Xiaoteng Ding, Jiahao Lin, Xinsheng Wang, Yuanhong Xu, Ruijie Deng, Jinghong Li
Summary: Fungicide abuse can lead to the emergence of fungicide-resistant fungal pathogens, posing a threat to agriculture and food safety. In this study, researchers developed iARMS, an isothermal amplification refractory mutation system, which enables rapid and sensitive detection of fungicide-resistant crop fungal pathogens. The iARMS assay showed high specificity and sensitivity, and it was able to detect rare fungicide-resistant isolates.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
M. G. Rizzo, S. Carnazza, L. M. De Plano, D. Franco, M. S. Nicol, V Zammuto, S. Petralia, Giovanna Calabrese, C. Gugliandolo, S. Conoci, S. P. P. Guglielmino
Summary: The study developed a new method for rapid and sensitive detection of bacteria in blood using engineered M13 phages and PCR in a miniaturized silicon microchip, providing a fast and efficient diagnosis approach.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Food Science & Technology
Mei Xie, Tianlan Chen, Xiong Xin, Zongwei Cai, Cheng Dong, Bo Lei
Summary: A method using a digital microfluidic chip and loop-mediated isothermal amplification technology was developed for multiplex detection of foodborne pathogens. The method showed higher sensitivity, simpler operation, higher efficiency, and smaller size compared to the commercial benchtop LAMP method.
Article
Chemistry, Multidisciplinary
Bong Kyun Kim, Sang-A Lee, Minju Park, Eui Ju Jeon, Mi Jung Kim, Jung Min Kim, Heesuk Kim, Seungwon Jung, Sang Kyung Kim
Summary: This article introduces a photonic PCR platform using hydrogel microparticles, which utilizes photothermal nanomaterials as heating elements for rapid and portable POCT. The researchers successfully demonstrate the ability to perform multiplex assays by loading multiple encoded pPIN microparticles in a single reaction.
Article
Virology
Brigitte Sigrist, Jessica Geers, Sarah Albini, Dennis Rubbenstroth, Nina Wolfrum
Summary: Avian bornaviruses were first identified as causative agents of a disease in parrots in 2008. Currently, detection of these viruses is primarily done through RT-PCR, but existing methods have limitations in specificity and breadth of detection. A new multiplex real-time RT-PCR assay has been developed to overcome these limitations and simultaneously detect and differentiate avian bornaviruses.
Article
Food Science & Technology
Eun-Ji Shin, Soo-Jeong Lee, Hoon-Jae Jeong, Ju-Hoon Lee
Summary: A novel multiplex real-time PCR method was developed for rapid and accurate serogrouping of Escherichia coli O-serotypes, enabling identification in food environments.
Article
Multidisciplinary Sciences
Li Sun, David G. Meckes
Summary: The new immuno-PCR method utilizes TotalSeq antibodies containing DNA conjugated oligos for high sensitivity and specificity profiling of multiple EV surface proteins simultaneously. This method has been successfully applied to cell and serum samples, and a micro-size exclusion chromatography method has been developed to detect EV surface proteins in as little as 10 μl of human serum when combined with immuno-PCR. Overall, this approach allows for rapid detection of multiple EV markers from small sample volumes in a single tube.
SCIENTIFIC REPORTS
(2021)
Article
Entomology
Carlos Aguirre, Evelyn Sanchez, Natalia Olivares, Patricio Hinrichsen
Summary: This study successfully developed a fast and sensitive detection protocol for Naupactus cervinus and Naupactus xanthographus, achieving 100% accuracy in a panel of 480 test samples. The protocol is based on multiplex TaqMan Real-time polymerase chain reaction and allows for species-specific detection with high sensitivity.
JOURNAL OF ECONOMIC ENTOMOLOGY
(2021)
Article
Entomology
Nicola Bodino, Vincenzo Cavalieri, Crescenza Dongiovanni, Anna Simonetto, Matteo Alessandro Saladini, Elisa Plazio, Gianni Gilioli, Giulia Molinatto, Maria Saponari, Domenico Bosco
Summary: The study examined the dispersal of Philaenus spumarius adults in different agroecosystems in Italy and found that the diffusion coefficient estimate for P. spumarius was higher in the meadow compared to the olive grove. The median distance from the release point for 1 day of dispersal was 26 m in the olive grove and 35 m in the meadow. The research also estimated that the dispersal of P. spumarius is limited to some hundreds of meters throughout the whole year, although it can be influenced by the structure of the agroecosystem.
ENVIRONMENTAL ENTOMOLOGY
(2021)
Editorial Material
Plant Sciences
Elia Choueiri, Fouad Jreijiri, Maria Saponari, Raied Abou Kubaa
JOURNAL OF PLANT PATHOLOGY
(2021)
Article
Multidisciplinary Sciences
Asmae Jlilat, Rosa Ragone, Stefania Gualano, Franco Santoro, Vito Gallo, Leonardo Varvaro, Piero Mastrorilli, Maria Saponari, Franco Nigro, Anna Maria D'Onghia
Summary: The study revealed that Xylella-infected plants showed higher levels of malic acid, formic acid, mannitol, and sucrose in their leaves, while slightly lower levels of oleuropein, with mannitol potentially playing a central role in resisting bacterial infection. This research contributes to identifying a set of metabolites that could serve as markers for X. fastidiosa infections in olive trees.
SCIENTIFIC REPORTS
(2021)
Article
Multidisciplinary Sciences
David Kottelenberg, Lia Hemerik, Maria Saponari, Wopke van der Werf
Summary: The study analyzed the spread of olive quick decline syndrome in Puglia, Italy, estimating the shape and movement rate of the disease front. The estimated rate of movement was 10.0 km per year, higher than previous expert judgements. The model suggests that the disease spread started around 2008.
SCIENTIFIC REPORTS
(2021)
Editorial Material
Plant Sciences
Mohamed Afechtal, Giuliana Loconsole, Maria Saponari, Raied Abou Kubaa
JOURNAL OF PLANT PATHOLOGY
(2021)
Article
Biochemical Research Methods
Sabrina Di Masi, Giuseppe E. De Benedetto, Cosimino Malitesta, Maria Saponari, Cinzia Citti, Giuseppe Cannazza, Giuseppe Ciccarella
Summary: The study identified potential biomarkers for Olive quick decline syndrome (OQDS) by analyzing metabolic profile differences in infected and healthy olive tree leaf samples. The findings suggest disturbances in flavonoid and long-chain fatty acid metabolism in infected samples, indicating a decrease in host defense capabilities. Additionally, oleic acid was confirmed as a putative diffusible signal factor (DSF), highlighting the power of LC-HRMS-based metabolomics in plant disease biomarker discovery.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Entomology
Nicola Bodino, Vincenzo Cavalieri, Mattia Pegoraro, Giuseppe Altamura, Francesca Canuto, Stefania Zicca, Giulio Fumarola, Rodrigo P. P. Almeida, Maria Saponari, Crescenza Dongiovanni, Domenico Bosco
Summary: This study investigated the transmission biology of Xf by the spittlebug Philaenus spumarius to olive trees, revealing that the vector is competent throughout its adult life, with bacterial load increasing in the foregut initially before stabilizing. Transmission rates may vary with season and climate conditions, and differences in vector survival can impact Xf spread among olive plants. These findings can enhance pathogen spread modeling and aid in the design of effective control measures against this vector-borne disease.
ENTOMOLOGIA GENERALIS
(2021)
Review
Microbiology
Massimiliano Morelli, Jose Manuel Garcia-Madero, Angeles Jos, Pasquale Saldarelli, Crescenza Dongiovanni, Magdalena Kovacova, Maria Saponari, Alberto Banos Arjona, Evelyn Hackl, Stephen Webb, Stephane Compant
Summary: Since 2013, Xylella fastidiosa has been reported to infect multiple hosts in various areas of Europe, causing severe damage to olive orchards in southern Apulia, Italy, resulting in the death of millions of trees. European and national measures have been implemented to reduce the spread of the pathogen and the associated olive quick decline syndrome. Research has been conducted to find better solutions to directly combat the bacterium and its main insect vector, Philaenus spumarius.
Editorial Material
Plant Sciences
Raied Abou Kubaa, Elia Choueiri, Khaled Heinoun, Fabrizio Cillo, Maria Saponari
JOURNAL OF PLANT PATHOLOGY
(2022)
Article
Agronomy
Giuliana Loconsole, Stefania Zicca, Lorenzo Manco, Oumaima El Hatib, Giuseppe Altamura, Oriana Potere, Vito Elicio, Franco Valentini, Donato Boscia, Maria Saponari
Summary: Two distinct protocols were optimized to detect X. fastidiosa in plant materials, with high diagnostic sensitivity values achieved using serological and molecular tests. The accuracy of the newly developed protocols was assessed based on ratios of healthy and infected plant tissues, showing qPCR consistently yielding the highest performance values regardless of host species.
Review
Plant Sciences
Blanca B. Landa, Maria Saponari, Oseias R. Feitosa-Junior, Annalisa Giampetruzzi, Filipe J. D. Vieira, Eliana Mor, Silke Robatzek
Summary: Xylella fastidiosa is a pathogenic bacterium that causes significant crop diseases and has seen a notable increase in occurrences worldwide. Recent studies have shed light on its interactions with different host plants, the plant immune system, and its impact on the host's microbiome.
Editorial Material
Plant Sciences
Annalisa Giampetruzzi, Giuliana Loconsole, Stefania Zicca, Donato Boscia, Giorgio Mariano Balestra, Maria Saponari
Article
Agronomy
Vincenzo Cavalieri, Angelo G. Delle Donne, Maria Saponari, Mauro Carrieri, Donato Boscia, Crescenza Dongiovanni
Summary: Infestations of Arge scita were first recorded in Italy in summer 2022, with almond trees in the Salento peninsula of the Apulia region showing severe leaf damages caused by the sawfly larvae. Molecular and morphological characterization confirmed the presence of A. scita on the infested trees. Further investigations are needed to understand the lifecycle, host range, and potential threat posed by this phytophagous insect in almond cultivation areas.
Review
Plant Sciences
Nicola Bodino, Vincenzo Cavalieri, Crescenza Dongiovanni, Maria Saponari, Domenico Bosco
Summary: The spatial-temporal dynamics and transmission biology of spittlebug populations play a crucial role in understanding the disease epidemiology of Xylella fastidiosa in Apulian olive groves. The spread of the pathogen is influenced by various factors, such as pathogen colonization, vector acquisition and inoculation, vector population dynamics, and dispersal. Despite advances in research on X. fastidiosa vectors in Europe, uncertainties remain, hindering a comprehensive understanding of pathogen epidemiology and effective management strategies. This review aims to provide a structured overview of key traits of X. fastidiosa vectors in the Apulian epidemic, identify knowledge gaps, and stimulate further research on X. fastidiosa pathosystems in Europe.
Article
Multidisciplinary Sciences
P. J. Zarco-Tejada, T. Poblete, C. Camino, V. Gonzalez-Dugo, R. Calderon, A. Hornero, R. Hernandez-Clemente, M. Roman-ecija, M. P. Velasco-Amo, B. B. Landa, P. S. A. Beck, M. Saponari, D. Boscia, J. A. Navas-Cortes
Summary: Plant pathogens, such as Xylella fastidiosa, are increasingly threatening global food security, causing significant yield losses. Spectral screening methods are crucial for early detection of non-visual symptoms of infection and prevention of spread. Using airborne spectroscopy, researchers have identified host-specific spectral pathways that can distinguish biotic-induced symptoms, improving detection accuracy and reducing crop losses worldwide.
NATURE COMMUNICATIONS
(2021)
Article
Biochemical Research Methods
Atif Khurshid Wani, Chirag Chopra, Daljeet Singh Dhanjal, Nahid Akhtar, Himanshu Singh, Poorvi Bhau, Anjuvan Singh, Varun Sharma, Rafael Silvio Bonilha Pinheiro, Juliana Heloisa Pine Americo-Pinheiro, Reena Singh
Summary: Zoonotic viral infections pose significant threats to global public health. Understanding the origins and dynamics of these infections is crucial for prevention and management of future outbreaks. Metagenomics is a powerful tool for studying the diversity of viral populations and potential zoonotic events.
JOURNAL OF VIROLOGICAL METHODS
(2024)
Article
Biochemical Research Methods
Michel V. F. Sucupira, Ana P. C. Argondizzo, Mariana Miguez, Anna E. de Araujo, Leila B. R. Silva, Marcelle B. Mello, Christiane F. S. Marques, Danielle Brito Cunha, Renata C. Bastos, Vanessa S. de Paula, Luciane A. Amado Leon
Summary: This study focuses on improving the purification, solubility, and protein expression levels of recombinant VP1-2A, a hepatitis A virus (HAV) structural protein with immunogenic activity. The results show that the modified approach successfully produces biologically active HAV VP1-2A, which can be used as a diagnostic tool for HAV infection.
JOURNAL OF VIROLOGICAL METHODS
(2024)
Article
Biochemical Research Methods
Arun K. Dhar, Roberto Cruz-Flores, Hung N. Mai, Janet Warg
Summary: A sensitive and robust TaqMan PCR method for detecting IHHNV in three commercially important shrimp species was developed and compared with other published methods. Multiple primer/probe sets, including qIH-Fw/qIH-Rv and 3144F/3232R, are recommended for the detection of IHHNV. These findings are valuable for large-scale screening of shrimp using a TaqMan real-time PCR assay.
JOURNAL OF VIROLOGICAL METHODS
(2024)
Article
Biochemical Research Methods
Kemal Mehinagic, Matthias Liniger, Maksym Samoilenko, Nick Soltermann, Markus Gerber, Nicolas Ruggli
Summary: A plasmid-based reporter assay has been developed for sensitive detection and titration of infectious African swine fever virus (ASFV). This novel method allows accurate measurement of ASFV titers and is applicable to ASFV of different genotypes, virulence, and sources.
JOURNAL OF VIROLOGICAL METHODS
(2024)
Article
Biochemical Research Methods
Jan Van Slambrouck, Charlotte Schoenaers, Lies Laenen, Xin Jin, Kurt Beuselinck, Ann Verdonck, Joost Wauters, Geert Molenberghs, Bart M. Vanaudenaerde, Robin Vos, Peter Mombaerts, Katrien Lagrou, Laurens J. Ceulemans
Summary: The ability to detect SARS-CoV-2 RNA or antigen in BAL fluid samples using point-of-care tests (POCTs) can reduce the risk of developing severe COVID-19 after lung transplantation.
JOURNAL OF VIROLOGICAL METHODS
(2024)