Journal
JOURNAL OF VIROLOGICAL METHODS
Volume 163, Issue 2, Pages 492-494Publisher
ELSEVIER
DOI: 10.1016/j.jviromet.2009.10.024
Keywords
HPV 52; HPV variants; Real-time PCR; TaqMan; LightCycler; Cervical cancer
Funding
- Slovenian Research Agency [Z3-0220-0381-08]
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A novel TaqMan real-time PCR (RT-PCR) assay for sensitive and specific detection of HPV 52 infection and confirmation/exclusion of the presence of HPV 52 in clinical specimens positive with a Roche HPV Linear Array cross-reactive probe was developed. Sensitivity of the assay at a 95% detection level was 3.9 DNA copies/reaction and the dynamic range was seven orders of magnitude, discriminating 10-10(7) viral genome equivalents/reaction. Testing 45 HPV-DNA negative samples and 102 HPV-DNA positive samples (42 HPV 52 positive samples and 60 samples containing 30 other HPV genotypes), showed complete agreement with results obtained with GP5+/GP6+ or PGMY09/PGMY0911 PCR-based screening and sequencing. Of the 27 HPV 31/33/58 positive samples cross-reacting with the Linear Array HPV 52 probe, 4 (14.8%) were identified as also containing HPV 52 using RT-PCR. All 16 Linear Array true HPV 52-positive samples were confirmed to contain HPV 52 DNA in RT-PCR testing. (C) 2009 Elsevier B.V. All rights reserved.
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