Article
Biochemistry & Molecular Biology
Yifen Wang, Jingjing Niu, Minmin Sun, Ziyi Li, Xiangyuan Wang, Yan He, Jie Qi
Summary: Establishing fast, sensitive, and convenient detection methods for S. iniae is of great significance for the early diagnosis and control of the disease in the global aquaculture industry and for humans dealing with raw fish. In this study, two methods of real-time MIRA and MIRA-LFD were established for the simA gene of S. iniae, which could complete amplification at a constant temperature of 42°C within 20 min. Real-time MIRA and MIRA-LFD assays showed high sensitivity, specificity, repeatability, simplicity, and rapidity for detecting S. iniae.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Fisheries
Venkata Satyanarayana Nallala, M. Makesh, K. Radhika, T. Sathish Kumar, P. Raja, R. Subburaj, M. Kailasam, K. K. Vijayan
Summary: In this study, nervous necrosis virus was successfully isolated from asymptomatic wild Asian seabass, and its genome was analyzed. The virus showed infectivity to Asian seabass larvae, causing 100% mortality, making it a potential candidate for developing a vaccine against viral nervous necrosis.
Article
Fisheries
Zhiqi Zhang, Jing Xing, Xiaoqian Tang, Xiuzhen Sheng, Heng Chi, Wenbin Zhan
Summary: This study manufactured abundant RGNNV VLPs and developed three monoclonal antibodies that can accurately detect the capsid protein of RGNNV and show potential in detection and tracking of the virus. Two of the antibodies also possess some neutralizing capacity against RGNNV infection.
Article
Fisheries
Weijun Mai, Bingjun Yan, Jian Xin
Summary: In this study, a vaccine utilizing Bacillus subtilis as a vehicle was developed to protect grouper against Nervous Necrosis Virus (NNV) infection. The vaccine induced enhanced non-specific immune responses, specific antibody production, and up-regulation of immune-related genes in the grouper. The highest dose of bath vaccination showed the most promising results, with significantly reduced mortality after NNV challenge. The vaccine demonstrated high levels of mucosal and humoral immunity and is a potential candidate for protecting against NNV infection.
Article
Fisheries
Menglan Zhang, Zhijie Lu, MeiZhen Tang, Gan Pan, Lijuan Zhao, Zhendong Qin, Li Lin
Summary: The study found that Epinephelus coioides mannose receptor (EcMR) responds to virus infection by modulating virus-induced apoptosis and inflammation.
Article
Virology
Zhiqi Zhang, Jing Xing, Xiaoqian Tang, Xiuzhen Sheng, Heng Chi, Wenbin Zhan
Summary: Nervous necrosis virus (NNV) is a significant pathogen in the aquaculture industry, causing substantial financial losses. The capsid protein (CP) is the only structural protein with pathogenicity and antigenicity, but information on immunodominant regions is scarce.
JOURNAL OF VIROLOGY
(2023)
Article
Fisheries
Min Yang, Qing Wang, Jinpeng Chen, Yuxin Wang, Yong Zhang, Qiwei Qin
Summary: The red-spotted grouper, a commercially important species in China and Southeast Asia, faces a serious threat from RGNNV disease outbreaks. By conducting a GWAS, researchers identified candidate genes involved in nervous system development, retinal formation, and lipid metabolism regulation, suggesting potential pathways to enhance disease resistance. This study provides insights for marker-assisted selection in breeding programs aiming to improve red-spotted grouper's resilience to RGNNV.
FISH & SHELLFISH IMMUNOLOGY
(2021)
Article
Forestry
Lu-Ke Zhang, Ke-Cheng Zhu, Hua-Yang Guo, Bao-Suo Liu, Bo Liu, Nan Zhang, Dian-Chang Zhang
Summary: A new cell line derived from the muscle tissue of the golden pompano, called golden pompano muscle (GPM), has been successfully developed and characterized in this study. GPM cells exhibit fibroblast-like morphology and have a high proliferation rate in Leibovitz's L-15 medium supplemented with fetal bovine serum. The GPM cell line has been stable for over 80 generations and can be cryopreserved. It has been confirmed that the GPM cells used in this study are from the golden pompano based on molecular characterization and chromosome analysis. In addition, GPM cells are susceptible to red-spotted grouper nervous necrosis virus (RGNNV) and can be used for gene expression studies and viral pathogenesis research. The interaction between host and virus can also be investigated using this cell line.
Article
Biochemistry & Molecular Biology
Siting Wu, Mengshi Sun, Xin Zhang, Jiaming Liao, Mengke Liu, Qiwei Qin, Jingguang Wei
Summary: The study characterized the EcTRAF4 homologue in orange-spotted grouper and found that it plays crucial roles in RGNNV infection process, promoting viral replication and interacting with RGNNV coat protein to inhibit IFN signaling and reduce inflammatory responses.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Immunology
Mengshi Sun, Siting Wu, Shaozhu Kang, Jiaming Liao, Luhao Zhang, Zhuqing Xu, Hong Chen, Linting Xu, Xin Zhang, Qiwei Qin, Jingguang Wei
Summary: This study found that the infection of red-spotted grouper nervous necrosis virus (RGNNV) induced the formation of stress granules (SGs) in grouper spleen cells. The SGs particles co-located with the classic SG marker protein eIF3 eta, and some SGs depolymerized under treatment with a translation inhibitor. Inhibition of the PERK kinase significantly inhibited the formation of SGs and decreased the phosphorylation of eIF2 alpha. Overexpression of G3BP1 in Epinephelus coioides inhibited the replication of RGNNV by up-regulating the interferon and inflammatory response.
FRONTIERS IN IMMUNOLOGY
(2022)
Review
Chemistry, Analytical
Poramin Boonbanjong, Kiatnida Treerattrakoon, Wassa Waiwinya, Piyawat Pitikultham, Deanpen Japrung
Summary: Isothermal amplification (IA) is a nucleic acid amplification technology that has significantly contributed to the healthcare system by improving disease diagnosis through higher sensitivity, specificity, and rapidity. Certain IA techniques provide a rapid, sensitive, specific, and simple method for disease diagnosis. However, not all IA techniques have been widely used in clinical applications due to the influence of different biomarkers and sample types on the IA system.
Article
Food Science & Technology
Ahtisham Ali, Jinfang Wang, Wei Jiang, Shuai Wei, Qinxiu Sun, Qiuyu Xia, Zefu Wang, Zongyuan Han, Yang Liu, Shucheng Liu
Summary: In this study, differential metabolites and potential biomarkers of hybrid grouper and golden pompano muscles were identified. Liquid chromatography-mass spectrometry (LC-MS) metabolomic analysis was performed, and principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA) were used to evaluate the results. The study found 1678 co-contained differential metabolites in positive modes and 1445 co-contained differential metabolites in negative modes, and determined 16 most differential metabolites as potential biomarkers in different muscle groups. The top 10 KEGG-enriched pathways were also identified.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2023)
Article
Fisheries
Xixi Guo, Wenji Wang, Qi Zheng, Qiwei Qin, Youhua Huang, Xiaohong Huang
Summary: This study compared the transcriptomic analysis of SGIV and RGNNV infected grouper cells, revealing the different cell death mechanisms and host immune responses caused by these two viruses. The results also showed the important roles of cytoskeleton and MAPK signaling pathways in SGIV infection, as well as the relationship between post-translational modifications of MAPK signaling-related proteins and viral replication and vacuole formation during RGNNV infection.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Xuzhuo Duan, Xianze Jia, Kaishan Liang, Fengqi Huang, Jinhong Shan, Huitao Chen, Xinhe Ruan, Lihua Li, Huihong Zhao, Qing Wang
Summary: In this study, the researchers successfully induced testis maturation in red-spotted grouper by injecting recombinant plasmids into the immature testis. The results showed that the testis developed from the immature to the mature state within 7 days after injection, and the spermatozoa concentration and motility in injected fish were comparable to naturally mature fish.
MARINE BIOTECHNOLOGY
(2022)
Article
Fisheries
Sumit Vinod Jungi, Pakkakul Sangsuriya, Suwimon Taengphu, Kornsunee Phiwsaiya, Molruedee Sonthi, Bunlung Nuangsaeng, Krishna R. Salin, Saengchan Senapin, Ha Thanh Dong
Summary: This study identified Nervous Necrosis Virus (NNV) of the RGNNV genotype as a virus associated with massive die-offs in imported pearl grouper fry in Thailand. The virus was found to localize in the vacuolation lesions of the central nervous system and retina of infected fish. Appropriate biosecurity measures are needed to prevent any loss to the marine aquaculture industry considering the infectious nature and broad host range of the virus.
Article
Biochemical Research Methods
Atif Khurshid Wani, Chirag Chopra, Daljeet Singh Dhanjal, Nahid Akhtar, Himanshu Singh, Poorvi Bhau, Anjuvan Singh, Varun Sharma, Rafael Silvio Bonilha Pinheiro, Juliana Heloisa Pine Americo-Pinheiro, Reena Singh
Summary: Zoonotic viral infections pose significant threats to global public health. Understanding the origins and dynamics of these infections is crucial for prevention and management of future outbreaks. Metagenomics is a powerful tool for studying the diversity of viral populations and potential zoonotic events.
JOURNAL OF VIROLOGICAL METHODS
(2024)
Article
Biochemical Research Methods
Michel V. F. Sucupira, Ana P. C. Argondizzo, Mariana Miguez, Anna E. de Araujo, Leila B. R. Silva, Marcelle B. Mello, Christiane F. S. Marques, Danielle Brito Cunha, Renata C. Bastos, Vanessa S. de Paula, Luciane A. Amado Leon
Summary: This study focuses on improving the purification, solubility, and protein expression levels of recombinant VP1-2A, a hepatitis A virus (HAV) structural protein with immunogenic activity. The results show that the modified approach successfully produces biologically active HAV VP1-2A, which can be used as a diagnostic tool for HAV infection.
JOURNAL OF VIROLOGICAL METHODS
(2024)
Article
Biochemical Research Methods
Arun K. Dhar, Roberto Cruz-Flores, Hung N. Mai, Janet Warg
Summary: A sensitive and robust TaqMan PCR method for detecting IHHNV in three commercially important shrimp species was developed and compared with other published methods. Multiple primer/probe sets, including qIH-Fw/qIH-Rv and 3144F/3232R, are recommended for the detection of IHHNV. These findings are valuable for large-scale screening of shrimp using a TaqMan real-time PCR assay.
JOURNAL OF VIROLOGICAL METHODS
(2024)
Article
Biochemical Research Methods
Kemal Mehinagic, Matthias Liniger, Maksym Samoilenko, Nick Soltermann, Markus Gerber, Nicolas Ruggli
Summary: A plasmid-based reporter assay has been developed for sensitive detection and titration of infectious African swine fever virus (ASFV). This novel method allows accurate measurement of ASFV titers and is applicable to ASFV of different genotypes, virulence, and sources.
JOURNAL OF VIROLOGICAL METHODS
(2024)
Article
Biochemical Research Methods
Jan Van Slambrouck, Charlotte Schoenaers, Lies Laenen, Xin Jin, Kurt Beuselinck, Ann Verdonck, Joost Wauters, Geert Molenberghs, Bart M. Vanaudenaerde, Robin Vos, Peter Mombaerts, Katrien Lagrou, Laurens J. Ceulemans
Summary: The ability to detect SARS-CoV-2 RNA or antigen in BAL fluid samples using point-of-care tests (POCTs) can reduce the risk of developing severe COVID-19 after lung transplantation.
JOURNAL OF VIROLOGICAL METHODS
(2024)
