Journal
JOURNAL OF VIROLOGICAL METHODS
Volume 167, Issue 1, Pages 103-106Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jviromet.2010.01.025
Keywords
HHV-6; Variant specific; Direct LAMP; HSCT
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A variant specific direct loop-mediated isothermal amplification (LAMP) method was developed to detect human herpesvirus-6 (HHV-6) variants in serum samples. Specific primers were designed against HHV-6 U86 gene. Initial validation analysis confirmed high specificity and sensitivity of the method. This method was shown to be highly reliable for monitoring active HHV-6 infection in hematopoietic stem cell transplant recipients. (C) 2010 Elsevier B.V. All rights reserved.
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