Reverse transcription loop-mediated isothermal amplification for the detection of highly pathogenic porcine reproductive and respiratory syndrome virus

A reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay targeting the open reading frames la of highly pathogenic porcine reproductive and respiratory syndrome virus genome was developed. The 10 reference strains, I clinical isolation strain and 122 positive samples were tested. Positive reactions were confirmed for all strains and specimens by reverse transcription loop-mediated isothermal amplification and nested reverse transcription polymerase chain reaction (RT-PCR). The results showed this detection technique is more reliable and convenient for rapid and sensitive diagnosis of highly pathogenic porcine reproductive and respiratory syndrome virus infection. (C) 2008 Elsevier B.V. All rights reserved.