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Food Science & Technology
Qiyuan Jing, Shijian Liu, Yang Song, Xiaoqi Tao
Summary: In this study, a TaqMan qPCR method based on the cytochrome b gene was established for quantitative detection of beef tallow. A normalized Delta Cq method based on Cyt b and 18S rDNA was also created for quantitative analysis of oils, which was validated on simulated oil samples.
Article
Virology
Yi Huang, Shuqi Xiao, Zhiming Yuan
Summary: The study compared and evaluated the sensitivity, reproducibility, and specificity of 23 in-house assays for ebolavirus detection, finding that targeting the more conservative NP gene may offer better sensitivity for EVD case definition and quantitative measurement. This analysis could contribute to the standardization of ebolavirus detection and quantification assays, providing a better understanding of results and making implementation easier, particularly during outbreaks.
Article
Agronomy
Jiao He, Mei-Ling Sun, De-Wei Li, Li-Hua Zhu, Jian-Ren Ye, Lin Huang
Summary: The TaqMan real-time PCR targeting the ApMat gene has the potential to provide rapid, specific, and reliable molecular detection of C. fructicola, C. gloeosporioides, C. siamense, and C. cangyuanense. This study also offers a reference solution for identifying species within the Colletotrichum gloeosporioides species complex (CGSC) that share similar morphological characteristics.
PEST MANAGEMENT SCIENCE
(2023)
Article
Microbiology
Alexandra Puertolas, Peter J. M. Bonants, Eric Boa, Steve Woodward
Summary: Numerous outbreaks of Phytophthora and Pythium diseases have occurred in Europe due to introduction of contaminated ornamental plants. Detection and identification of pathogens are crucial. Results showed that oomycetes were isolated from 89.9% of plants using classical techniques, and real-time PCR demonstrated that oomycetes were associated with all plants tested.
Article
Agriculture, Dairy & Animal Science
Min Zheng, Su Lin, Shizhong Zhang, Xiuqin Chen, Dandan Jiang, Shaoying Chen, Shao Wang, Shilong Chen
Summary: A novel TaqMan-based real-time PCR approach was developed to reliably detect and diagnose H146-like GCV, enabling real-time diagnosis of infections and providing a greater understanding of the epidemiology and clinical presentation of the disease.
Article
Veterinary Sciences
Siti Tasnim Makhtar, Sheau Wei Tan, Nur Amalina Nasruddin, Nor Azlina Abdul Aziz, Abdul Rahman Omar, Farina Mustaffa-Kamal
Summary: A specific TaqMan-based real-time RT-PCR assay targeting the N gene of FeMV was developed in this study, showing higher detection rate and sensitivity in early detection of FeMV infection.
BMC VETERINARY RESEARCH
(2021)
Article
Veterinary Sciences
Yuelin Liu, Libing Liu, Jinfeng Wang, Ting Wang, Yaxin Gao, Xiaoxia Sun, Wanzhe Yuan, Ruiwen Li, Jianchang Wang
Summary: In this study, a new Taqman-based real-time RT-PCR method was developed for the detection of Bovine kobuvirus (BKV), which can be used efficiently and specifically for BKV detection and epidemiological research.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Biotechnology & Applied Microbiology
Da Zhang, Junhuang Wu, Jianfei Sun, Caixia Bai, Fazhi Xu, Pengfei Duan, Yong Wang
Summary: A TaqMan-based real-time PCR assay was established to detect Duck circovirus, showing high sensitivity, specificity, and repeatability, making it suitable for clinical use.
Article
Microbiology
Amro Hashish, Avanti Sinha, Yuko Sato, Nubia R. Macedo, Mohamed El-Gazzar
Summary: This article describes the development of an improved detection method for Ornithobacterium rhinotracheale (ORT). The modified method exhibits higher efficiency, sensitivity, and specificity, enabling sensitive and efficient diagnosis of ORT from clinical samples.
Article
Biochemical Research Methods
Babu K. Nath, Shubhagata Das, Tridip Das, Jade K. Forwood, Shane R. Raidal
Summary: TaqMan qPCR is a robust and reliable technique for detecting and quantifying target DNA copies. This study reports the development and validation of a highly sensitive TaqMan qPCR for the detection of highly diverse PiCV in pigeon samples with excellent reproducibility, specificity, and sensitivity.
JOURNAL OF VIROLOGICAL METHODS
(2022)
Article
Agriculture, Dairy & Animal Science
Jinpeng Zhang, Huanru Fu, Cuiteng Chen, Jinxiu Jiang, Yusheng Lin, Bin Jiang, Lin Lin, Qilin Hu, Chunhe Wan
Summary: PiMeV infection was detected in fecal samples of pigeons and a sensitive and specific TaqMan-qPCR method was established. The study also suggests that PiMeV may be vertically transmitted in racing pigeon embryos.
Article
Fisheries
Han Lin, Jingyang Sun, Jie Ma, Zhendong Qin, Biao Jiang, Wei Li, Qing Wang, Youlu Su, Li Lin, Chun Liu
Summary: This study investigated the spread and replication of Klebsiella pneumoniae in infected bullfrog tissues. A qPCR assay was developed to detect and quantify the bacteria, with the spleen identified as the major site for K. pneumoniae replication. Additionally, the mucus assay showed potential as a noninvasive tool for the early surveillance and prevention of K. pneumoniae in bullfrogs.
Article
Food Science & Technology
Liang Guo, Chun-Dong Li, Guo-Qiang Liu, Jian-Xing Luo, Wei-Liang Xu, Yuan-Sheng Guo
Summary: This study investigated SNPs in the FecB locus of Xilingol indigenous sheep breeds using novel techniques, laying the groundwork for future authentication and breeding strategies.
FOOD SCIENCE & NUTRITION
(2022)
Article
Biochemical Research Methods
Zeinab Alsaheli, Ali Abdallah, Ornella Incerti, Ahmad Shalaby, Sahar Youssef, Michele Digiaro, Toufic Elbeaino
Summary: Singleplex and multiplex real-time RT-PCR assays were developed to detect seven fig-infecting viruses, and a large-scale survey in Egypt showed a high prevalence of mixed infections, with FMV being the most commonly observed virus.
JOURNAL OF VIROLOGICAL METHODS
(2021)
Article
Microbiology
Amro Hashish, Avanti Sinha, Amr Mekky, Yuko Sato, Nubia R. Macedo, Mohamed El-Gazzar
Summary: In this study, two TaqMan qPCR assays were developed and validated for the accurate detection of Bordetella avium, showing higher PCR efficiencies compared to the currently available TaqMan qPCR. The new assays correctly identified all BA isolates and exhibited negative results against other microorganisms, with high repeatability and reproducibility. These newly developed assays are reliable tools for the sensitive, specific, and efficient diagnosis of BA.
Article
Biotechnology & Applied Microbiology
Huifeng Peng, Dong Wei, Feng Chen, Gu Chen
JOURNAL OF APPLIED PHYCOLOGY
(2016)
Article
Biotechnology & Applied Microbiology
Huifeng Peng, Dong Wei, Gu Chen, Feng Chen
BIOTECHNOLOGY FOR BIOFUELS
(2016)
Article
Food Science & Technology
Fuhua Li, Gu Chen, Xiong Fu
FOOD AND BIOPROCESS TECHNOLOGY
(2016)
Article
Chemistry, Applied
Hong Wang, Junhong Wang, Caisheng Qiu, Yutong Ye, Xinbo Guo, Gu Chen, Tong Li, Yufu Wang, Xiong Fu, Rui Hai Liu
Article
Food Science & Technology
Fuhua Li, Bin Zhang, Gu Chen, Xiong Fu
FOOD RESEARCH INTERNATIONAL
(2017)
Review
Food Science & Technology
Fuhua Li, Gu Chen, Bin Zhang, Xiong Fu
FOOD RESEARCH INTERNATIONAL
(2017)
Article
Food Science & Technology
Fuhua Li, Bin Zhang, Gu Chen, Xiong Fu
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
(2017)
Article
Immunology
Shiqi Lin, Ling Yang, Gu Chen, Bing Li, Dingqiang Chen, Lin Li, Zhenbo Xu
MICROBIAL PATHOGENESIS
(2017)
Review
Food Science & Technology
Gu Chen, Congcong Chen, Zhonghua Lei
TRENDS IN FOOD SCIENCE & TECHNOLOGY
(2017)
Article
Food Science & Technology
Jing He, Congcong Chen, Qianxian He, Jingyu Li, Fan Ying, Gu Chen
FOOD RESEARCH INTERNATIONAL
(2019)
Article
Food Science & Technology
Fan Ying, Shiqi Lin, Jingyu Li, Xuewu Zhang, Gu Chen
Summary: MAOs, essential deamination enzymes for neurotransmitters and amines, are potential targets for treating mental and neurodegenerative disorders. By utilizing ultrafiltration purification and in silico prediction, inhibitory peptides targeting MAOs were identified from soybean protein hydrolysate, demonstrating potential as functional nutraceuticals for treating neurodegenerative and mental disorders, with PLYSN showing strong inhibition activity against MAO-B.
Article
Microbiology
Qianxian He, Jingyu Li, Yongkai Ma, Qi Chen, Gu Chen
Summary: The study is the first report on probiotic screening and characterization of bacteria from PCR-Chachiensis, revealing some bacteria with cholesterol-lowering potential and carbohydrate utilization capability.
Article
Biochemistry & Molecular Biology
Shiqi Lin, Shiliang Li, Tong Ouyang, Gu Chen
Summary: Excess ammonium causes toxicity and stress in cyanobacteria, and how cyanobacteria respond to ammonium stress is not well understood. In this study, a gene called Slr1821 was found to be a crucial regulator for ammonium stress response in Synechocystis sp. PCC6803. The expression of many genes involved in nitrogen uptake, assimilation, and carbon regulation depended on the presence of Slr1821, highlighting its importance in maintaining carbon/nitrogen balance under high nitrogen conditions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Agricultural Engineering
Baojun Zhu, Gu Chen, Xupeng Cao, Dong Wei
BIORESOURCE TECHNOLOGY
(2017)
Article
Biochemistry & Molecular Biology
Na Lu, Jun-Hui Chen, Dong Wei, Feng Chen, Gu Chen
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2016)
Article
Biochemical Research Methods
Atif Khurshid Wani, Chirag Chopra, Daljeet Singh Dhanjal, Nahid Akhtar, Himanshu Singh, Poorvi Bhau, Anjuvan Singh, Varun Sharma, Rafael Silvio Bonilha Pinheiro, Juliana Heloisa Pine Americo-Pinheiro, Reena Singh
Summary: Zoonotic viral infections pose significant threats to global public health. Understanding the origins and dynamics of these infections is crucial for prevention and management of future outbreaks. Metagenomics is a powerful tool for studying the diversity of viral populations and potential zoonotic events.
JOURNAL OF VIROLOGICAL METHODS
(2024)
Article
Biochemical Research Methods
Michel V. F. Sucupira, Ana P. C. Argondizzo, Mariana Miguez, Anna E. de Araujo, Leila B. R. Silva, Marcelle B. Mello, Christiane F. S. Marques, Danielle Brito Cunha, Renata C. Bastos, Vanessa S. de Paula, Luciane A. Amado Leon
Summary: This study focuses on improving the purification, solubility, and protein expression levels of recombinant VP1-2A, a hepatitis A virus (HAV) structural protein with immunogenic activity. The results show that the modified approach successfully produces biologically active HAV VP1-2A, which can be used as a diagnostic tool for HAV infection.
JOURNAL OF VIROLOGICAL METHODS
(2024)
Article
Biochemical Research Methods
Arun K. Dhar, Roberto Cruz-Flores, Hung N. Mai, Janet Warg
Summary: A sensitive and robust TaqMan PCR method for detecting IHHNV in three commercially important shrimp species was developed and compared with other published methods. Multiple primer/probe sets, including qIH-Fw/qIH-Rv and 3144F/3232R, are recommended for the detection of IHHNV. These findings are valuable for large-scale screening of shrimp using a TaqMan real-time PCR assay.
JOURNAL OF VIROLOGICAL METHODS
(2024)
Article
Biochemical Research Methods
Kemal Mehinagic, Matthias Liniger, Maksym Samoilenko, Nick Soltermann, Markus Gerber, Nicolas Ruggli
Summary: A plasmid-based reporter assay has been developed for sensitive detection and titration of infectious African swine fever virus (ASFV). This novel method allows accurate measurement of ASFV titers and is applicable to ASFV of different genotypes, virulence, and sources.
JOURNAL OF VIROLOGICAL METHODS
(2024)
Article
Biochemical Research Methods
Jan Van Slambrouck, Charlotte Schoenaers, Lies Laenen, Xin Jin, Kurt Beuselinck, Ann Verdonck, Joost Wauters, Geert Molenberghs, Bart M. Vanaudenaerde, Robin Vos, Peter Mombaerts, Katrien Lagrou, Laurens J. Ceulemans
Summary: The ability to detect SARS-CoV-2 RNA or antigen in BAL fluid samples using point-of-care tests (POCTs) can reduce the risk of developing severe COVID-19 after lung transplantation.
JOURNAL OF VIROLOGICAL METHODS
(2024)