Article
Biotechnology & Applied Microbiology
R. Aravintharaj, R. Asokan, K. Prasad Babu, M. Manamohan, Krishnan Nagendran
Summary: This study characterizes the Tomato spotted wilt virus (TSWV) isolate (Ka-To) infecting tomato from India through biological, serological, and molecular assays. The pathogenicity of TSWV (Ka-To) isolate was confirmed by mechanical inoculation on tomato, cowpea, and datura, resulting in necrotic or chlorotic local lesions. Serological assay and RT-PCR amplification confirmed the identity of TSWV. The obtained nucleotide sequences of Ka-To isolate showed similarity to TSWV isolates from Spain and Hungary, indicating reassortment and recombination in the genome.
Article
Microbiology
Wanhong Zhang, Yubing Jiao, Chengying Ding, Lili Shen, Ying Li, Yanbi Yu, Kun Huang, Bin Li, Fenglong Wang, Jinguang Yang
Summary: A sensitive detection system based on CRISPR/Cas13a was developed for rapid and accurate diagnosis of tomato spotted wilt virus (TSWV), demonstrating high selectivity and improved sensitivity compared to traditional RT-PCR methods.
FRONTIERS IN MICROBIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Shiming Qi, Shijie Zhang, Md. Monirul Islam, Ahmed H. El-Sappah, Fei Zhang, Yan Liang
Summary: The Tomato spotted wilt virus (TSWV) is highly destructive to tomato cultivation globally, with only the natural resistance gene Sw-5 demonstrating high resistance. Over time, however, resistance induced by Sw-5 weakens due to the global spread of TSWV, necessitating the exploration of other resistance genes and novel resources for breeding TSWV-resistant tomato varieties.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Agronomy
Tais M. F. Suassuna, Timothy Lane Grey, Xuelin Luo, Albert K. Culbreath, Cristiane Pilon
Summary: This study aimed to evaluate the vigor and germination of peanut seeds from different genotypes affected by Tomato spotted wilt disease (TSW). The results showed that TSW symptoms did not negatively affect the germination, vigor, and seed size of the seeds.
Article
Multidisciplinary Sciences
Jing Li, Si Chen, Run-Shuang Qiu, Li-Zhen Zhang, Yue Chen, Xue Zheng, Ting-Ting Li, Li-Hua Zhao, Zhong-Kai Zhang
Summary: This study found that the NSs protein of Tomato zonate spot virus acts as an RNA silencing suppressor in tobacco plants, inhibiting the transcription and protein expression of viral genes and alleviating the symptoms of viral infection. The study also discovered that the NSs protein suppresses the expression of the Zingipain-2-like protein, which co-localizes with NSs.
Article
Entomology
Xiaobin Zheng, Yanran Wan, Min Tao, Jiangjiang Yuan, Kun Zhang, Jing Wang, Youjun Zhang, Pei Liang, Qingjun Wu
Summary: The Obst protein plays an important role in the acquisition and transmission of Tomato spotted wilt orthotospovirus (TSWV) in Frankliniella occidentalis, while the ApoD and Orai proteins do not have this effect.
Article
Multidisciplinary Sciences
Juan Francisco Iturralde Martinez, Cristina Rosa
Summary: Detecting viruses in the early stages of infection is challenging due to their low abundance, making it difficult to use sensitive laboratory techniques. To overcome this, researchers have developed a portable and affordable system that can detect tomato spotted wilt orthotospovirus in real time using a technique called Recombinase Polymerase Amplification. This technique allows for detection directly from crude plant extracts without the need for nucleic acid extraction and can be visually identified by the formation of DNA-flocculus complexes. This system provides a practical solution for in-field virus detection and can be used by scientists and extension managers without the need for specialized laboratory facilities.
SCIENTIFIC REPORTS
(2023)
Article
Genetics & Heredity
Junheng Lv, Yunrong Mo, Minghua Deng, Junqiang Xu, Bin Xu, Xinyun Li, Jing Li, Caiqian Jiang, Ying Zhou, Ziran Wang, Zhengan Yang, Kai Zhao
Summary: In this study, a tomato inbred line, YNAU335, showed immunity to tomato spotted wilt virus (TSWV) at first but later lost its immunity. The researchers used various techniques to analyze the pathological features and genetic polymorphism of the TSWV infecting the tomato fruit. They discovered a mutated protein that likely caused the loss of immunity in YNAU335.
Article
Agriculture, Multidisciplinary
Baoan Song, Jiao Li, Ningning Zan, Hongfu He, Deyu Hu
Summary: A total of 35 piperazine derivatives were synthesized and assessed for their anti-tomato spotted wilt virus (TSWV) activity. Compounds 34 and 35 displayed significant potency against TSWV with EC50 values of 62.4 and 59.9 μg/mL, respectively, superior to the control agents ningnanmycin and ribavirin. Mechanistic studies revealed that compounds 34 and 35 inhibited TSWV infection by binding to the N protein, preventing assembly of the viral core structure ribonucleoprotein (RNP). Amino acid residue 94 on the N protein was identified as the key site for interaction between the compounds and the TSWV N target. This study highlights the potential of piperazine derivatives containing alpha-ketoamide moieties as antiviral agents.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Genetics & Heredity
Sirine Werghi, Frederic Aparicio Herrero, Hatem Fakhfakh, Faten Gorsane
Summary: Research has shown that tomato plants exhibit genotype-specific symptoms progression upon TSWV infection. The regulation of miRNA167a and ARF8 gene by TSWV disrupts plant growth intentionally to promote spread in sensitive cultivars, affecting auxin synthesis and shaping tomato responses to the virus. Modulation of the miR167a-ARF8 regulatory module could potentially be utilized in tomato resistance breeding programs.
Article
Agronomy
Junheng Lv, Minghua Deng, Shurui Jiang, Haishan Zhu, Zuosen Li, Ziran Wang, Jing Li, Zhengan Yang, Yanling Yue, Junqiang Xu, Kai Zhao
Summary: This study identified the gene SlCHS3 as a key factor in the resistance of tomato plants to Tomato spotted wilt virus. The regulation of flavonoid synthesis plays a significant role in improving resistance to the virus.
MOLECULAR BREEDING
(2022)
Article
Plant Sciences
Sun-Jung Kwon, Young-Eun Cho, Oh-Hun Kwon, Hyung-Gon Kang, Jang-Kyun Seo
Summary: This study identified a breakthrough TSWV variant (TSWV-YI) in pepper crops in South Korea, with phylogenetic and recombination analyses showing it to be a reassortant variant incorporating RNA segments from both local and foreign sources. The emergence of TSWV-YI, which infected previously resistant pepper cultivars, highlights the potential threat of resistance-breaking variants to sustainable pepper production in South Korea, necessitating the development of management strategies.
Article
Biochemistry & Molecular Biology
Chen Yang, Chengming Yu, Zhenjia Zhang, Deya Wang, Xuefeng Yuan
Summary: This study identified the molecular characteristics of subgenomic RNAs (sgRNAs) in Tomato spotted wilt virus (TSWV) that contribute to viral protein expression. The 3' ends of sgRNAs have A-rich characteristics and are conserved among different TSWV isolates. Additionally, the untranslated regions (UTRs) of sgRNA-Ns can enhance cap-dependent translation. These findings enrich the understanding of sgRNAs in ambisense RNA viruses.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Junheng Lv, Minghua Deng, Zuosen Li, Haishan Zhu, Ziran Wang, Yanling Yue, Zhengan Yang, Junqiang Xu, Shurui Jiang, Wei Zhao, Jing Li, Kai Zhao
Summary: In this study, genes and metabolites related to TSWV resistance in tomato plants were identified and studied using transcriptomic and metabolomic techniques. The results revealed that these genes and metabolites are mainly involved in plant hormone signal transduction and flavonoid synthesis pathways. Nucleotide-binding site-leucine-rich repeat genes and transcription factors were also found to potentially be involved in the response to TSWV.
HORTICULTURAL PLANT JOURNAL
(2023)
Article
Plant Sciences
Edgar Antonio Rodriguez-Negrete, Enrique Alejandro Guevara-Rivera, Angela Paulina Arce-Leal, Norma Elena Leyva-Lopez, Jesus Mendez-Lozano
Summary: The nonstructural protein NSm of tomato spotted wilt virus (TSWV) has been identified as the avirulence determinant of the tomato single dominant Sw-5 resistance gene. However, resistance-breaking (RB) isolates with specific point mutations in the NSm viral protein have been observed. In this study, a novel TSWV-RB Mexican isolate with a noncanonical C118F substitution in the NSm protein was discovered, highlighting a previously undescribed viral adaptation.
MOLECULAR PLANT PATHOLOGY
(2023)
Article
Biochemical Research Methods
Atif Khurshid Wani, Chirag Chopra, Daljeet Singh Dhanjal, Nahid Akhtar, Himanshu Singh, Poorvi Bhau, Anjuvan Singh, Varun Sharma, Rafael Silvio Bonilha Pinheiro, Juliana Heloisa Pine Americo-Pinheiro, Reena Singh
Summary: Zoonotic viral infections pose significant threats to global public health. Understanding the origins and dynamics of these infections is crucial for prevention and management of future outbreaks. Metagenomics is a powerful tool for studying the diversity of viral populations and potential zoonotic events.
JOURNAL OF VIROLOGICAL METHODS
(2024)
Article
Biochemical Research Methods
Michel V. F. Sucupira, Ana P. C. Argondizzo, Mariana Miguez, Anna E. de Araujo, Leila B. R. Silva, Marcelle B. Mello, Christiane F. S. Marques, Danielle Brito Cunha, Renata C. Bastos, Vanessa S. de Paula, Luciane A. Amado Leon
Summary: This study focuses on improving the purification, solubility, and protein expression levels of recombinant VP1-2A, a hepatitis A virus (HAV) structural protein with immunogenic activity. The results show that the modified approach successfully produces biologically active HAV VP1-2A, which can be used as a diagnostic tool for HAV infection.
JOURNAL OF VIROLOGICAL METHODS
(2024)
Article
Biochemical Research Methods
Arun K. Dhar, Roberto Cruz-Flores, Hung N. Mai, Janet Warg
Summary: A sensitive and robust TaqMan PCR method for detecting IHHNV in three commercially important shrimp species was developed and compared with other published methods. Multiple primer/probe sets, including qIH-Fw/qIH-Rv and 3144F/3232R, are recommended for the detection of IHHNV. These findings are valuable for large-scale screening of shrimp using a TaqMan real-time PCR assay.
JOURNAL OF VIROLOGICAL METHODS
(2024)
Article
Biochemical Research Methods
Kemal Mehinagic, Matthias Liniger, Maksym Samoilenko, Nick Soltermann, Markus Gerber, Nicolas Ruggli
Summary: A plasmid-based reporter assay has been developed for sensitive detection and titration of infectious African swine fever virus (ASFV). This novel method allows accurate measurement of ASFV titers and is applicable to ASFV of different genotypes, virulence, and sources.
JOURNAL OF VIROLOGICAL METHODS
(2024)
Article
Biochemical Research Methods
Jan Van Slambrouck, Charlotte Schoenaers, Lies Laenen, Xin Jin, Kurt Beuselinck, Ann Verdonck, Joost Wauters, Geert Molenberghs, Bart M. Vanaudenaerde, Robin Vos, Peter Mombaerts, Katrien Lagrou, Laurens J. Ceulemans
Summary: The ability to detect SARS-CoV-2 RNA or antigen in BAL fluid samples using point-of-care tests (POCTs) can reduce the risk of developing severe COVID-19 after lung transplantation.
JOURNAL OF VIROLOGICAL METHODS
(2024)