Article
Genetics & Heredity
Abhishek Singh, Mukesh Thakur, Lalit Kumar Sharma, Dhriti Banerjee
Summary: Species assignment using DNA analysis is a routine procedure for wildlife cases, but mitochondrial markers often fail or provide biased results in identifying hybrids or making assignments at sub-species level. In this study, genome sequencing identified a rescued lion cub as a hybrid of Asiatic and African lion, contributing to the operating procedures of wildlife forensics.
Article
Veterinary Sciences
Dongwei Yuan, Zihan Yan, Mingyue Li, Yi Wang, Mingjun Su, Dongbo Sun
Summary: Transmissible gastroenteritis virus (TGEV) is a major pathogen of viral enteritis and diarrhea in suckling piglets. A study on the TGEV strain HQ2016 showed that it is more similar to the TGEV Purdue cluster and can cause clinical symptoms in inoculated piglets.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Biophysics
Sophie Krokhine, Hadis Torabi, Ali Doostmohammadi, Pouya Rezai
Summary: The COVID-19 pandemic has highlighted the threat of infectious diseases to public health and safety, especially in developing countries. Airborne viruses like SARS-CoV-2 are difficult to control and require rapid detection methods for effective containment and precaution implementation.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2021)
Article
Agriculture, Dairy & Animal Science
Enrica Sozzi, Davide Lelli, Ilaria Barbieri, Chiara Chiapponi, Ana Moreno, Tiziana Trogu, Giovanni Tosi, Antonio Lavazza
Summary: In this study, a paramyxovirus was identified in a sample from a wild boar carcass imported from Australia in 2004. The virus showed antigenic and morphological similarities with other members of the Paramyxoviridae family. Phylogenetic analysis revealed that the virus belonged to genotype A of bovine parainfluenza virus type 3 (BPIV-3) and was closely related to two swine paramyxoviruses (SPMV) isolated from encephalitic pigs in the United States. The high sequence identity to a bovine respirovirus suggests potential cross-species transmission from cattle to wild boars.
Article
Veterinary Sciences
Hui-Hua Zheng, Yi-Lin Bai, Tong Xu, Lan-Lan Zheng, Xin-Sheng Li, Hong-Ying Chen, Zhen-Ya Wang
Summary: This study investigated the biological characteristics of reemerging pseudorabies virus (PRV) strains in central China. The PRV isolates showed closer genetic relationship to Chinese PRV variants post-2012, with distinct differences from earlier Chinese PRV isolates. The isolates displayed amino acid variations compared to early Chinese PRV strains and European-American PRV strains, shedding light on the evolution of PRV strains.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Correction
Biotechnology & Applied Microbiology
Gurusamy Raman, Kyu Tae Park, Joo-Hwan Kim, SeonJoo Park
Summary: A revised version of this article has been published and is available through the original article.
Article
Neurosciences
Demi Oddes, Ayanda Ngwenya, Illke B. Malungo, Anita Burkevica, Therese Hard, Mads F. Bertelsen, Muhammad A. Spocter, D. Michael Scantlebury, Paul R. Manger
Summary: Using orexin-A immunohistochemistry, the distribution, morphology, and nuclear parcellation of orexinergic neurons in the hypothalami of Asiatic lions, African lions, and Southeast African cheetahs were studied. The clustering and distribution of orexinergic neurons in these felids appear to be more complex than in other mammals.
JOURNAL OF COMPARATIVE NEUROLOGY
(2023)
Article
Multidisciplinary Sciences
Aiping Song, Jiangshuo Su, Haibin Wang, Zhongren Zhang, Xingtan Zhang, Yves van de Peer, Fei Chen, Weimin Fang, Zhiyong Guan, Fei Zhang, Zhenxing Wang, Likai Wang, Baoqing Ding, Shuang Zhao, Lian Ding, Ye Liu, Lijie Zhou, Jun He, Diwen Jia, Jiali Zhang, Chuwen Chen, Zhongyu Yu, Daojin Sun, Jiafu Jiang, Sumei Chen, Fadi Chen
Summary: Chrysanthemum is a globally important ornamental plant with great economic, cultural, and symbolic value. This study reports the near-complete assembly and annotation of the chrysanthemum genome, revealing its complex genetic background and the presence of whole-genome triplication and lineage-specific polyploidization events. The identified genes associated with key ornamental traits can be used for chrysanthemum genetic improvement.
NATURE COMMUNICATIONS
(2023)
Article
Veterinary Sciences
Xue Mi, Chunjie Yang, Ying Lu, Hejie Wang, Qiuying Qin, Ronglin Chen, Zhenkong Chen, Yunyan Luo, Ying Chen, Zuzhang Wei, Weijian Huang, Kang Ouyang
Summary: This study isolated and purified an EV-G strain from piglets in Guangxi Province, China, which showed stable cytopathic effects on Marc-145 cells and pathogenicity to neonatal piglets. Infection with the CH/17GXQZ/2017 strain significantly decelerated the weight gain of suckling piglets.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Immunology
Gani Asa Dudin, Ibrahim M. M. Aziz, Rasha M. M. Alzayed, Anwar Ahmed, Tajamul Hussain, Ali M. M. Somily, Muslim M. M. Alsaadi, Fahad N. N. Almajhdi
Summary: The presence of a large foreign workforce and the annual pilgrimage in Saudi Arabia has contributed to the emergence and diversity of respiratory viruses. This study focused on the H3N2 subtype of influenza A virus (IAV) in clinical samples from Riyadh, Saudi Arabia. The analysis revealed amino acid substitutions in the H3N2 strains that were not present in the current vaccine strains, emphasizing the need for regular monitoring of vaccine efficacy and the potential for designing novel and population-based vaccines.
Article
Virology
Ming Jie Lim, Zhi Yang Loh, Hui Ling Yeo, Surya Pavan Yenamandra, Marcella Kong, Hao Yang Pang, Meng Han Lee, Mahathir Humaidi, Cliff Chua, Jane Griffiths, Lee Ching Ng, Hapuarachchige Chanditha Hapuarachchi, Diyar Mailepessov
Summary: This study reports the genetic characterization and first isolation of JEV from mosquito pools in Singapore. The findings indicate the presence of genotype I of JEV and suggest multiple introductions and potential establishment of an enzootic cycle in the local fauna.
Article
Veterinary Sciences
Quang Lam Truong, Hoang Minh Duc, Tuan Nguyen Anh, Yen Nguyen Thi, Thang Nguyen Van, Phuong Hoang Thi, Huong Nguyen Thi Thu, Lan Nguyen Thi
Summary: In Vietnam, the predominant antigenic type of CDV is the Asia-1 variant, which shows notable regional and genetic variation. Vaccinated dogs had significantly lower neutralization ability against field CDV strains in northern and central Vietnam.
RESEARCH IN VETERINARY SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
David Diaz-Escandon, Gulnara Tagirdzhanova, Dan Vanderpool, Carmen C. G. Allen, Andre Aptroot, Oluna Ceska, David L. Hawksworth, Alejandro Huereca, Kerry Knudsen, Jana Kocourkova, Robert Luecking, Philipp Resl, Toby Spribille
Summary: Ascomycota account for a significant proportion of named fungal species. By analyzing previously undersampled and uncultured lineages, researchers have discovered a new clade within Pezizomycotina that diverged over 300 million years ago. This clade, known as Lichinomycetes, includes diverse symbionts, endophytes, and mycorrhizae, and challenges traditional taxonomic classifications.
Article
Infectious Diseases
Ahmed Magdy Khalil, Isshu Kojima, Wataru Fukunaga, Misuzu Okajima, Sumire Mitarai, Yoshikazu Fujimoto, Tsutomu Matsui, Masakazu Kuwahara, Tatsunori Masatani, Kosuke Okuya, Makoto Ozawa
Summary: By improving the method for isolating avian influenza viruses (AIVs) from environmental water samples, we were able to significantly increase the recovery efficiency and isolate a larger number of AIVs from water samples collected at a wintering site. Genetic analysis showed that multiple genetic constellations of the same AIV subtypes were circulating in a single winter season.
TRANSBOUNDARY AND EMERGING DISEASES
(2022)
Article
Genetics & Heredity
Hui He, Tao Wang, Chuyu Tang, Zhengfei Cao, Xiaojian Pu, Yuling Li, Xiuzhang Li
Summary: This study annotated and analyzed the chloroplast genomes of three Saussurea plants, and identified potential molecular markers and phylogenetic relationships. The results provide important references for the identification and evolutionary studies of Saussurea plants.
Article
Cell Biology
Huildore Bommanna Ranjitha, Veena Ammanathan, Neha Guleria, Madhusudan Hosamani, B. Parameshwaraiah Sreenivasa, Valiya Valappil Dhanesh, Rashmi Santhoshkumar, B. K. Chandrasekhar Sagar, Bishnu Prasad Mishra, Raj Kumar Singh, Aniket Sanyal, Ravi Manjithaya, Suresh H. Basagoudanavar
Summary: Foot-and-mouth disease virus (FMDV) induces UPR and enhances functional autophagy through the PERK-mediated pathway, with inhibition of BiP alleviating infection. Modulation of UPR and autophagy can reduce viral progeny numbers by enhancing interferon response.
JOURNAL OF CELL SCIENCE
(2021)
Article
Engineering, Environmental
Rani Rai, Raj Kumar Singh, Surindra Suthar
Summary: Composting toxic weed Lantana camara foliage with cow dung resulted in increased nutrient content and decreased organic carbon. The compost showed prevention against Escherichia coll. and Salmonella, while exhibiting strong pathogen-inhibiting properties. GC MS analysis revealed the presence of biocidal compounds in the Lantana compost, suggesting its potential as a bio-manure with biopesticide properties for sustainable organic farming.
JOURNAL OF HAZARDOUS MATERIALS
(2021)
Article
Infectious Diseases
Shyam Singh Dahiya, Saravanan Subramaniam, Jitendra Kumar Biswal, Biswajit Das, Bikash Ranjan Prusty, Syed Zeeshan Ali, Sagar Ashok Khulape, Jajati Keshari Mohapatra, Raj Kumar Singh
Summary: Research in India has shown that foot-and-mouth disease (FMD) is primarily caused by the ME-SA topotype of the FMDV serotype O, with the O/ME-SA/Ind2001e lineage being the most prevalent during 2014-2018. Further assessment is needed to understand the spread of the new O/ME-SA/2018 cluster, while the Indian vaccine strain O/INDR2/1975 has been found to provide adequate antigenic coverage against emerging and prevalent O serotype lineages.
TRANSBOUNDARY AND EMERGING DISEASES
(2021)
Article
Biotechnology & Applied Microbiology
V. Balamurugan, S. R. A. Thirumalesh, A. Alamuri, S. SowjanyaKumari, K. Vinod Kumar, L. Linshamol, V. Bharath, M. Nagalingam, P. Roy
Summary: The study aimed to express and evaluate recombinant outer membrane proteins of Leptospira for use as diagnostic antigens in the LAT test for detecting anti-leptospiral antibodies. Results showed that both rLoa22 and rOmpL37 had high diagnostic sensitivity and specificity in the LAT.
LETTERS IN APPLIED MICROBIOLOGY
(2021)
Article
Biochemical Research Methods
Ritesh Sharma, Sameer Shrivastava, Sanjay Kumar Singh, Abhinav Kumar, Sonal Saxena, Raj Kumar Singh
Summary: The study introduces Deep-ABPpred, a deep learning classifier for identifying ABPs in protein sequences, which outperforms other state-of-the-art classifiers. This model shows high precision and applicability in proposing and synthesizing novel ABPs for experimentation. Deep-ABPpred demonstrates the capability of identifying potent antibacterial peptides in protein sequences, confirming its potential impact in the field of antimicrobial research.
BRIEFINGS IN BIOINFORMATICS
(2021)
Article
Biochemical Research Methods
V Balamurugan, Bibitha Varghese, S. Sowjanya Kumari, K. Vinod Kumar, D. Muthuchelvan, M. Nagalingam, Parimal Roy
Summary: The study developed an ABrAC ELISA method for detecting PPRV antigens in clinical specimens of sheep and goats with high diagnostic sensitivity and specificity.
JOURNAL OF VIROLOGICAL METHODS
(2021)
Article
Virology
Vinayagamurthy Balamurugan, Kirubakaran Vinod Kumar, Ramesh Dheeraj, Rashmi Kurli, Kuralayanapalya Puttahonnappa Suresh, GurrappaNaidu Govindaraj, Bibek Ranjan Shome, Parimal Roy
Summary: The study used national surveillance data to understand the temporal and spatial epidemiology of PPR in India. Analysis from 1995 to 2019 showed PPR as a significant disease in sheep and goats, with outbreaks occurring round the year and most frequently in winter. Since 2011, outbreaks have progressively declined in most regions of India.
Article
Biochemical Research Methods
V Balamurugan, Bibitha Varghese, S. SowjanyaKumari, K. Vinod Kumar, D. Muthuchelvan, M. Nagalingam, D. Hemadri, Parimal Roy, B. R. Shome
Summary: The study developed a novel immuno-diagnostic assay ABrC-ELISA for detecting PPRV antibodies in sheep and goats, with high sensitivity and specificity demonstrated in a known standard panel of sera. The assay showed rapid, sensitive, and specific characteristics, making it a better alternative for serosurveillance of PPR in both endemic and non-endemic countries.
JOURNAL OF VIROLOGICAL METHODS
(2021)
Article
Infectious Diseases
Sreenivasa Beeragere Parameshwaraiah, Jajati K. Mohapatra, Veena Jumanal, Dhanesh Valia Valappil, Saravanan Subramaniam, Bhimnere Hanumanthagouda Manjunatha Patel, Suresh H. Basagoudanavar, Madhusudan Hosamani, Brahmadev Pattnaik, Raj Kumar Singh, Aniket Sanyal
Summary: Recent field outbreak strains of foot-and-mouth disease virus serotype A in India have shown considerable antigenic drift from the vaccine strain, necessitating the selection of a new strain. The new candidate strain, A IND 27/2011, showed variation in key antigenic amino acid positions compared to the vaccine strain A IND 40/2000, but demonstrated suitability as an effective vaccine strain with strong adaptability, immunogenicity, and potency in cattle.
TRANSBOUNDARY AND EMERGING DISEASES
(2022)
Article
Agriculture, Dairy & Animal Science
Vinayagamurthy Balamurugan, Anusha Alamuri, Kirubakaran Vinod Kumar, Bibitha Varghese, Gurrappanaidu Govindaraj, Divakar Hemadri, Parimal Roy
Summary: This study investigated the seroprevalence of leptospirosis in small ruminants in the coastal districts of South Peninsular India, revealing a high prevalence and diverse distribution of serovars, which can be valuable for diagnostic reference panels in laboratories.
Article
Virology
Monu Karki, Gnanavel Venkatesanna, Amit Kumar, A. B. Pandey
Summary: In this study, the chemokine-binding protein (CBP) and the GM-CSF/IL-2 inhibition factor (GIF) of orf virus (ORFV) isolates from sheep and goats in different regions of India were analyzed. Both proteins play a crucial role in establishing short-term immunity and aiding re-infection in their host. The study also identified potential therapeutic applications of these proteins in inflammatory diseases caused by the overexpression of specific cytokines.
ARCHIVES OF VIROLOGY
(2022)
Article
Biochemistry & Molecular Biology
Chandan Dharmashekar, Bhargav Shreevatsa, Anisha S. Jain, Bhavana Harendra, Sushma Pradeep, Prashanth M. Vishwanath, Pranav Singh, V Balamurugan, K. K. Vinod, Sharanagouda S. Patil, Ali A. Shati, Mohammad Y. Alfaifi, Serag Eldin I. Elbehairi, Raghavendra G. Amachawadi, Shiva Prasad Kollur, Chandan Shivamallu
Summary: A pseudopeptide was found to be highly effective in inhibiting the growth of Leptospira, suggesting its potential as an alternative drug for the treatment of leptospirosis.
Article
Agriculture, Dairy & Animal Science
V. Balamurugan, K. Vinod Kumar, Anusha Alamuri, P. P. Sengupta, G. Govindaraj, B. R. Shome
Summary: This study aimed to estimate the prevalence of Toxoplasma gondii antibodies in dairy cattle with reproductive problems and identified the coexistence of other pathogens. The results revealed the widespread presence of these pathogens in cattle and their association with reproductive disorders.
Review
Immunology
Krithiga Natesan, Shrikrishna Isloor, Balamurugan Vinayagamurthy, Sharada Ramakrishnaiah, Rathnamma Doddamane, Anthony R. Fooks
Summary: Rabies is an ancient disease with a long history, and efforts to prevent it have been made since the first interactions between humans and dogs. In the past 100 years, significant progress has been made in developing vaccines for both humans and animals, with next-generation vaccines showing increased immunogenicity and clinical efficacy.
Article
Chemistry, Analytical
Satheesh Natarajan, Jayaraj Joseph, Balamurugan Vinayagamurthy, Pedro Estrela
Summary: The clinical manifestation of leptospirosis is often misdiagnosed as other febrile illnesses, making a need for a precise molecular diagnostic tool to detect the lipL32 gene. The developed CRISPR dFnCas9-based quantitative lateral flow immunoassay showed excellent performance with a low-cost lateral flow device and a lowest detectable limit of 1 fg/mL for leptospirosis diagnosis.
