Journal
JOURNAL OF VETERINARY MEDICAL SCIENCE
Volume 76, Issue 1, Pages 139-143Publisher
JAPAN SOC VET SCI
DOI: 10.1292/jvms.13-0274
Keywords
antioxidant activity; Babesia gibsoni; canine; peroxiredoxin
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Funding
- Global COE program
- Ministry of Education, Culture, Sports, Science and Technology, Japan
- Grants-in-Aid for Scientific Research [24780295, 13J05843, 24658242] Funding Source: KAKEN
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Peroxiredoxins (Prxs) are a family of antioxidant enzymes. Here, we cloned a 2-Cys Prx, BgTPx-1, from the canine Babesia parasite B. gibsoni. Sequence identity between BgTPx-1 and 2-Cys Prx of B. bovis was 81% at the amino acid level. Enzyme activity assay by using recombinant BgTPx-1 (rBgTPx-1) indicated that BgTPx-1 has antioxidant activity. Antiserum from a mouse immunized with rBgTPx-1 reacted with parasite lysates and detect a protein with a monomeric size of 22 kDa and also a 44 kDa protein, which might be an inefficiently reduced dimer. BgTPx-1 was expressed in the cytoplasm of B. gibsoni merozoites. These results suggest that the BgTPx-1 may play a role to control redox balance in the cytoplasm of B. gibsoni.
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