Review
Biochemistry & Molecular Biology
Lucie Larigot, Daniel Mansuy, Ilona Borowski, Xavier Coumoul, Julien Dairou
Summary: Caenorhabditis elegans is an important model used in biological research, with 76 genes encoding cytochromes P450 in its genome. Research has shown that P450s play a role in various biological functions of C. elegans, but more studies are needed to fully understand their biochemical properties.
Review
Toxicology
Slobodan P. Rendic, F. Peter Guengerich
Summary: This overview discusses the metabolic activation of various compounds by cytochrome P450 enzymes, with a focus on bioactivation reactions. The study identifies a higher level of activation for general chemicals, drugs, and natural products compared to physiological compounds. Enzymes such as P450 3A4, 1A2, 2E1, and 1A1 are highlighted for their roles in catalyzing the formation of activated metabolites from different compound groups.
ARCHIVES OF TOXICOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Andrew D. Huber, William C. Wright, Wenwei Lin, Kinjal Majumder, Jonathan A. Low, Jing Wu, Cameron D. Buchman, David J. Pintel, Taosheng Chen
Summary: This study found that mutating a single residue in the PXR receptor can convert the antagonist SPA70 into an agonist. Molecular dynamics simulations revealed that in certain cases, the action of SPA70 is similar to that of agonists, further elucidating the relationship between the structure and activity of the PXR receptor.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Yousef M. Al-saraireh, Fatemah O. F. O. Alshammari, Omar H. Abu-azzam, Sa'ed M. Al-dalain, Yahya M. Al-sarayra, Mansour Haddad, Hafiz Makeen, Aiman Al-Qtaitat, Mohammad Almermesh, Sameeh A. Al-sarayreh
Summary: This review aims to investigate the differential expression of the cytochrome P450 enzyme family in ovarian cancer and its impact on treatment. It is of significance for understanding the pathogenesis of ovarian cancer and developing new therapeutic methods.
Review
Pharmacology & Pharmacy
Mahmoud A. El-Ghiaty, Sara R. El-Mahrouk, Mohammed A. Alqahtani, Ayman O. S. El-Kadi
Summary: Arsenic is a hazardous heavy metalloid that poses threats to human health worldwide. It can be found in the environment in various forms. Arsenic-based compounds, either inorganic (iAs) or organoarsenicals (oAs), can cause biochemical derangements in living systems, particularly affecting the network of cytochrome P450 enzymes (CYPs), which play a crucial role in metabolism. This review focuses on non-human models to gain a comprehensive understanding of possible CYP alterations in response to arsenic exposure.
DRUG METABOLISM REVIEWS
(2023)
Article
Biochemistry & Molecular Biology
Marta Nowak, Katarzyna Zawadzka, Janusz Szemraj, Aleksandra Goralczyk-Binkowska, Katarzyna Lisowska
Summary: This study investigated the biodegradation mechanism of chloroxylenol by fungal strains and analyzed the ecotoxicity of emerging by-products. The results showed a high elimination rate of PCMX by the tested microorganisms, and the identification of new fungal by-products. Furthermore, the study revealed a detoxifying nature of the biodegradation process of PCMX.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biodiversity Conservation
M. G. Gantchoff, J. D. Erb, D. M. MacFarland, D. C. Norton, J. L. Price Tack, B. J. Roell, J. L. Belant
Summary: Cougars have the potential for re-establishment in certain regions, with a need for greater understanding of potential distribution and connectivity to inform management and policy decisions. The Great Lakes region of the USA is projected to be an important area for cougar range expansion into the Midwestern and Eastern USA. Conservation efforts for large carnivores like cougars should focus on protecting habitat patches, linkages, and promoting long-term coexistence.
BIOLOGICAL CONSERVATION
(2021)
Review
Biochemistry & Molecular Biology
Annika Roeder, Saskia Huesken, Michael C. Hutter, Allan E. Rettie, Helmut Hanenberg, Constanze Wiek, Marco Girhard
Summary: The mammalian cytochrome P450 monooxygenase CYP4B1 has the ability to activate a wide range of xenobiotics, leading to tissue-specific toxicities. It can also hydroxylate fatty acids and fatty alcohols. Recent crystal structures of native rabbit CYP4B1 and the p.E310A variant have provided insights into its enigmatic role in xenobiotic and endobiotic metabolism, as well as its unusual heme-binding characteristics. Although CYP4B1 does not play a major role in hepatic P450-catalyzed drug metabolism, its expression patterns have been associated with various cancers and potential roles in cancer development. This review will summarize the current research on CYP4B1, focusing on its roles in endogenous and exogenous compound metabolism, structural properties, cancer association, and potential application in targeted cancer therapy with suicide gene approaches.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Agriculture, Dairy & Animal Science
Elisa Scarsella, J. Skyla Fay, Guillaume Jospin, Jessica K. Jarett, Zhandra Entrolezo, Holly H. Ganz
Summary: This study compares the gut microbiomes of healthy domestic ferrets and cats, finding significant differences between the two. Ferrets exhibit a higher representation of Firmicutes and Proteobacteria, while cats have a higher prevalence of Bacteroidota and Actinomycetota. The ferrets' microbiomes have lower alpha diversities. The research highlights the potential need for tailored gastrointestinal care for ferrets.
Review
Virology
John S. Munday, Neroli A. Thomson
Summary: Papillomaviruses can cause warts in humans and animals, and are also linked to cancer. In cats, the number of virus-related lesions has increased in recent years. Research covers the virus life cycle, associated lesions, and vaccination for prevention in domestic cats.
Article
Multidisciplinary Sciences
Laurentijn Tilleman, Kaat Rubben, Wim Van Criekinge, Dieter Deforce, Filip Van Nieuwerburgh
Summary: This study tested the effectiveness of Targeted Locus Amplification (TLA) combined with Illumina or Nanopore sequencing in accurate genotyping and phasing of pharmacogenes. The results showed limited nucleotide mapping on target and incomplete coverage of genes with both sequencing methods. However, the optimized primer pair for the CYP2D6 gene in the Nanopore dataset resulted in correct haplotypes, indicating the potential reliability of this method with patient-specific primer design and optimization.
SCIENTIFIC REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Freeborn Rwere, Sangchoul Im, Lucy Waskell
Summary: By altering the 140s FMN binding loop of CYPOR, the activity with cyt P450s can be affected, with the mutant activity primarily determined by its ability to reduce ferric cyt P450. The different behaviors of the mutants are mainly due to the altered structure and mobility of the loop.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Lulu Ni, Ping Miao, Jian Jiang, Fang Wan, Jiangan Li, Min Ai, Lingzhong Kong, Su Tu
Summary: This study found that Glycyrrhiza promotes the metabolism of toxic components of Aconitum by upregulating the expression of CYP3A, reducing the content of heart failure-related markers, and improving the myocardial energy metabolism disorder, thus alleviating the development of congestive heart failure.
Article
Veterinary Sciences
Kraisiri Khidkhan, Saranya Poapolathep, Sittinee Kulprasertsri, Rattapong Sukkheewan, Paphatsara Khunlert, Mario Giorgi, Amnart Poapolathep
Summary: This study investigated the comparative capacity of in vitro biotransformation of fipronil among chicken, duck, quail, goose, and rat. The results showed that chicken and duck are more efficient in metabolizing fipronil to sulfone compared to quail, goose, and rat. The study also suggested that fipronil sulfone may serve as a biomarker to indicate fipronil exposure in quail, goose, and rat.
JOURNAL OF VETERINARY SCIENCE
(2022)
Article
Chemistry, Multidisciplinary
Alecia R. Gee, Isobella S. J. Stone, Tegan P. Stockdale, Tara L. Pukala, James J. De Voss, Stephen G. Bell
Summary: We have achieved efficient oxidation reactions on unactivated carbon-hydrogen bonds by modifying the enzyme structure.
CHEMICAL COMMUNICATIONS
(2023)
Article
Pharmacology & Pharmacy
Yasuhiro Uno, Hiroshi Yamazaki
Article
Pharmacology & Pharmacy
Yasuhiro Uno, Shotaro Uehara, Takashi Inoue, Shu Kawamura, Norie Murayama, Miyu Nishikawa, Shinichi Ikushiro, Erika Sasaki, Hiroshi Yamazaki
BIOCHEMICAL PHARMACOLOGY
(2020)
Article
Pharmacology & Pharmacy
Yasuhiro Uno, Norie Murayama, Hiroshi Yamazaki
BIOCHEMICAL PHARMACOLOGY
(2020)
Article
Pharmacology & Pharmacy
Makiko Shimizu, Yasuhiro Uno, Masahiro Utoh, Hiroshi Yamazaki
DRUG METABOLISM AND PHARMACOKINETICS
(2020)
Article
Pharmacology & Pharmacy
Yasuhiro Uno, Hiroshi Yamazaki
DRUG METABOLISM AND PHARMACOKINETICS
(2020)
Article
Pharmacology & Pharmacy
Shotaro Uehara, Yasuhiro Uno, Hiroshi Yamazaki
DRUG METABOLISM AND PHARMACOKINETICS
(2020)
Article
Pharmacology & Pharmacy
Shotaro Uehara, Yasuhiro Uno, Hiroshi Yamazaki
DRUG METABOLISM AND PHARMACOKINETICS
(2020)
Article
Veterinary Sciences
Tetsuya Komatsu, Kennosuke Sugie, Naoko Inukai, Osamu Eguchi, Toshifumi Oyamada, Hiroshi Sawada, Noriko Yamanaka, Tomoyuki Shibahara
JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION
(2020)
Article
Pharmacology & Pharmacy
Yasuhiro Uno, Shotaro Uehara, Norie Murayama, Makiko Shimizu, Hiroshi Yamazaki
BIOCHEMICAL PHARMACOLOGY
(2020)
Article
Pharmacology & Pharmacy
Yasuhiro Uno, Takahiro Mikami, Yasuko Tsukazaki, Yasuharu Nakanishi, Norie Murayama, Shinichi Ikushiro, Hideshi Tsusaki, Hiroshi Yamazaki
Summary: In cynomolgus and rhesus macaques, genetic variants in UGT1A1/6/9 genes were identified, including unique variants specific to each species. The study suggests that these variants, such as UGT1A1G69R, may affect the glucuronidation of drugs mediated by UGT1A1 in these macaque species.
Article
Pharmacology & Pharmacy
Yasuhiro Uno, Shotaro Uehara, Norie Murayama, Hiroshi Yamazaki
Summary: Genetic variants in AOX1 were identified and allele frequencies were assessed in cynomolgus and rhesus macaques. The study found that AOX1 is polymorphic in these non-human primate species, suggesting potential impacts on drug metabolism that require further investigation.
Article
Pharmacology & Pharmacy
Yurie Okawa, Sakae Kohara, Asako Uchiyama, Hiroshi Yamazaki, Yasuhiro Uno
Summary: The biodistribution profile of cell-based therapy products in animal models is crucial for evaluating their safety and efficacy, with qPCR being a useful method for detecting and quantifying xenogeneic cell-derived DNA. The use of the DUF1220 gene for analysis showed promising results in differentiating human genome from mouse and cynomolgus monkey genomes, providing potential for future biodistribution studies.
DRUG METABOLISM AND PHARMACOKINETICS
(2021)
Article
Veterinary Sciences
Tetsuya Komatsu, Erina Yoshida, Ayumi Shigenaga, Nozomi Yasuie, Shintaro Uchiyama, Yuji Takamura, Kennosuke Sugie, Kumiko Kimura, Makoto Haritani, Tomoyuki Shibahara
Summary: The study found that two calves died due to Klebsiella pneumoniae infection leading to bacterial meningoencephalitis, primarily attributed to multidrug-resistant Klebsiella and immunosuppression. This provides new insights into Klebsiella pneumoniae infections in cattle.
JOURNAL OF VETERINARY MEDICAL SCIENCE
(2021)
Article
Veterinary Sciences
Tetsuya Komatsu, Takashi Kubo, Rena Kitou, Naomi Kawamoto, Masaji Mase, Yu Yamamoto, Tomoyuki Shibahara
JOURNAL OF VETERINARY MEDICAL SCIENCE
(2020)
Correction
Pharmacology & Pharmacy
S. Uehara, Y. Uno, T. Inoue, M. Kawano, M. Shimizu, A. Toda, M. Utoh, E. Sasaki, H. Yamazaki
DRUG METABOLISM AND DISPOSITION
(2020)
