Article
Biochemical Research Methods
Jianlin Zhang, Dengfeng Cheng, Jiuyang He, Juanji Hong, Chang Yuan, Minmin Liang
Summary: Ferritins are iron storage proteins that can be used for targeted delivery of anticancer drugs and diagnostic molecules. This study provides detailed procedures for loading different types of cargoes into ferritin nanocages for controlled delivery.
Article
Chemistry, Multidisciplinary
Xiuhua Pan, Xiaochen Pei, Haiqin Huang, Nan Su, Ziheng Wu, Zhenghong Wu, Xiaole Qi
Summary: In this study, a novel approach using tetralysine modified H-chain ferritin as packaging material for individualized CRISPR/Cas9 delivery was developed. This method showed higher genome editing efficiency both in vitro and in vivo, likely due to enhanced endosomal escape and pH-induced disassembly abilities of this packaging material. These results suggest potential advantages for intracellular delivery of CRISPR/Cas9.
JOURNAL OF CONTROLLED RELEASE
(2021)
Article
Biochemistry & Molecular Biology
Ayush K. Srivastava, Paolo Arosio, Maura Poli, Fadi Bou-Abdallah
Summary: A unique approach using a novel plasmid design allows the synthesis of complex ferritin nanostructures, with the ability to easily tune the H to L subunit ratio for the synthesis of various heteropolymer ferritins.
JOURNAL OF MOLECULAR BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Yushun Li, Xinyu Wang, Qinghua Zhang, Yuanbo Shen, Jin Wang, Shiming Qi, Pan Zhao, Tayeb Muhammad, Md. Monirul Islam, Xiangqiang Zhan, Yan Liang
Summary: In this study, a gene related to chlorophyll content and chloroplast ultrastructure in a yellow stigma mutant of tomato was identified through map-based cloning. This gene encodes a magnesium chelatase H subunit and its mutation leads to the yellow stigma phenotype. In addition, the functional complementation test confirmed the function of this gene.
Article
Pharmacology & Pharmacy
Xiuhua Pan, Nan Su, Jiachen Li, Haiqin Huang, Ziheng Wu, Kang Sha, Jiaojiao Chen, Zhenghong Wu, Xiaole Qi
Summary: By genetically modifying H-chain apoferritin with tetralysine at each subunit to create TL-HFn, a novel approach was explored to enhance the lysosomal escape performance and nuclear accumulation of chemotherapy drugs, showing promising potential for ultimate nucleus delivery. The constructed TL-HFn demonstrated better performance in escaping lysosomes and accumulating DOX in the nucleus, indicating potential for application in delivering chemotherapy drugs effectively.
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Ippei Inoue, Mayumi Chiba, Kenichiro Ito, Yoriko Okamatsu, Yasuyo Suga, Yoshiro Kitahara, Yuichi Nakahara, Yuta Endo, Kazutoshi Takahashi, Uno Tagami, Naofumi Okamoto
Summary: A novel, high-yield method for preparing high drug-loaded ferritin nanomedicine has been established, allowing for the successful introduction of various types of drug molecules into ferritin nano-cages. By incubating ferritin with a target drug at an appropriate pH, a complex is formed which enables efficient drug loading and higher drug recovery rates. This simple one-step method shows promise as a powerful production process for constructing drug-loaded ferritin carriers for drug delivery systems.
Article
Biochemistry & Molecular Biology
Jaswir Basran, Elizabeth S. Booth, Laura P. Campbell, Sarah J. Thackray, Mehul H. Jesani, Jonathan Clayden, Peter C. E. Moody, Christopher G. Mowat, Hanna Kwon, Emma L. Raven
Summary: 【】The kynurenine pathway is the major route of tryptophan metabolism, catalyzed by heme-dependent dioxygenase enzymes. The crystal structure of a bacterial TDO in complex with L-kynurenine is presented in this paper, revealing a mechanism for the hydrolysis of NFK in the active site. Hydrogen bonding interactions and heme 7-propionate anchor the L-kynurenine molecule into the pocket.
JOURNAL OF INORGANIC BIOCHEMISTRY
(2021)
Article
Chemistry, Analytical
Lei Yang, Xu Sun, Dong Wei, Huangxian Ju, Yu Du, Hongmin Ma, Qin Wei
Summary: An AIECL bioconjugate was developed by encapsulating Ir(ppy)(3) complexes in apoFt, enhancing ECL emission and enabling sensitive detection of CYFRA 21-1. This study demonstrates the potential for utilizing apoFt in designing iridium(III)-based AIECL emitters for innovative ECL immunoassays.
ANALYTICAL CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Maddalena Di Sanzo, Flora Cozzolino, Anna Martina Battaglia, Ilenia Aversa, Vittoria Monaco, Alessandro Sacco, Flavia Biamonte, Camillo Palmieri, Francesca Procopio, Gianluca Santamaria, Francesco Ortuso, Piero Pucci, Maria Monti, Maria Concetta Faniello
Summary: The study found that the interaction between FTH1 and PRDX6 in cancer cells can affect cell proliferation and migration, leading to a less invasive phenotype.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Martin Kondza, Marta Mandic, Ivona Ivancic, Sanda Vladimir-Knezevic, Ivica Brizic
Summary: The study aimed to investigate the inhibitory effect of Artemisia annua extract on CYP2B6 and CYP3A4 enzymes, as well as the type of inhibition, the presence of reversible or pseudo-irreversible inhibition, and the possible heme destruction. The methanolic extract of Artemisia annua showed significant inhibition on CYP2B6 (by almost 90%) and CYP3A4 enzymes (by almost 70%). It also exhibited irreversible inhibition, suggesting potential interactions with drugs and dietary supplements.
Article
Chemistry, Multidisciplinary
Hai Chen, Liang Ma, Yuhao Zhang
Summary: Functional nanoparticles with high peroxidase-like activity can be easily synthesized through enzymatic catalysis by utilizing the intrinsic ferroxidase activity and mineralization capability of ferritin. These reconstituted nanoparticles show comparable catalytic activity with a natural enzyme, horse radish peroxidase, allowing for the construction of a dual-enzyme sensor system for the detection of biomolecules. The biocompatibility and easy modification of the ferritin shell make these nanoparticles encapsulated by a protein shell have great potential application in theranostics and immunoassays.
Article
Chemistry, Multidisciplinary
Christian A. Gomez, Dibyendu Mondal, Qian Du, Natalie Chan, Jared C. Lewis
Summary: Directed evolution was used to engineer variants of SadX, resulting in site-selective azidation and succinylation of amino acids and amines. This study provides a promising starting point for evolving SadX variants that can functionalize structurally distinct substrates and enable enzymatic C-H functionalization with non-native functional groups.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Multidisciplinary Sciences
Sachio Shibata, Satoshi Sogabe, Masanori Miwa, Takuya Fujimoto, Nobuyuki Takakura, Akihiko Naotsuka, Shuji Kitamura, Tomohiro Kawamoto, Tomoyoshi Soga
Summary: LDHA and LDHB are two isoforms of lactate dehydrogenase with different roles in cancer therapy and cell autophagy. The selective inhibitor AXKO-0046 developed in this study effectively inhibits LDHB activity and has potential applications in cancer metabolism research.
SCIENTIFIC REPORTS
(2021)
Article
Chemistry, Multidisciplinary
Victor Yin, Paul W. A. Devine, Janet C. Saunders, Arjan Barendregt, Fiona Cusdin, Alexandra Ristani, Alistair Hines, Sam Shepherd, Marcin Dembek, Claire L. Dobson, Joost Snijder, Nicholas J. Bond, Albert J. R. Heck
Summary: Native mass spectrometry is a powerful tool for studying biomacromolecular assemblies. However, the conventional assumption that all peaks in a mass spectrum can be attributed to an underlying charge state distribution breaks down for certain types of macromolecular protein complexes. We propose a novel analytical framework that combines high-resolution native mass spectrometry, single particle Orbitrap-based charge detection mass spectrometry, and sophisticated spectral simulations to accurately interpret and extract mass information from these distorted spectra.
Article
Multidisciplinary Sciences
Antonia Grauel, Jan Kaegi, Tim Rasmussen, Iryna Makarchuk, Sabrina Oppermann, Aurelien F. A. Moumbock, Daniel Wohlwend, Rolf Mueller, Frederic Melin, Stefan Guenther, Petra Hellwig, Bettina Boettcher, Thorsten Friedrich
Summary: Bacterial pathogens possess resistance to cellular stressors through terminal bd oxidases. The authors presented the structure of E. coli bd-II oxidase and the inhibitor aurachin D, which could be utilized for the development of specifically binding antibiotics.
NATURE COMMUNICATIONS
(2021)