Article
Immunology
A. Elisabeth Gressler, Sabrina Luebke, Bettina Wagner, Corinna Arnold, Katharina L. Lohmann, Christiane L. Schnabel
Summary: Equine asthma, a common disease affecting approximately 20% of horses, shows similarities to human asthma and is believed to be immune-mediated. By analyzing the cell populations in the lower airways of horses with different severities of equine asthma, researchers identified characteristic changes in the cell compositions, highlighting potential differences between severe cases and mild to moderate cases as well as healthy horses. These findings contribute to a better understanding of the pathogenesis of equine asthma.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Pharmacology & Pharmacy
Justyna Kobryn, Bartosz Raszewski, Tomasz Zieba, Witold Musial
Summary: The aim of this study was to investigate the application of newly synthesized starch derivatives as excipients in hydrophilic gel for prolonging drug release. The modified starch with citric acid was found to effectively prolong the release of lidocaine hydrochloride from methylcellulose gel.
Article
Chemistry, Medicinal
Mohammed Gamal, Ibrahim A. Naguib, Rehab M. Abdelfatah
Summary: A new chromatographic method was introduced to simultaneously estimate three drugs in a veterinary formulation, successfully determining their concentrations and purities without interference from other additives. This method shows potential for application in quality control laboratories for purity testing and concentration assays of pure drugs and commercial formulations.
ARCHIV DER PHARMAZIE
(2021)
Article
Pharmacology & Pharmacy
Urszula Adamiak-Giera, Anna Nowak, Wiktoria Duchnik, Paula Ossowicz-Rupniewska, Anna Czerkawska, Anna Machoy-Mokrzynska, Tadeusz Sulikowski, Lukasz Kucharski, Marta Bialecka, Adam Klimowicz, Monika Bialecka
Summary: The study evaluated the in vitro penetration of ketoprofen and lidocaine hydrochloride through human skin using a transdermal vehicle. It was found that ketoprofen had higher penetration from the transdermal vehicle compared to commercial preparations, and the addition of lidocaine did not affect the permeation. The inclusion of certain excipients, such as capsicum tincture, menthol, and camphor, increased the penetration of both ketoprofen and lidocaine. This alternative method shows potential for patients with multiple diseases and polypragmasy.
FRONTIERS IN PHARMACOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Qian Yuan, Xiaojin Wang, Zhanglin Li, Wenzhuo Guo, Hua Cheng, Qingdong Cao
Summary: We analyzed the microbial mNGS results of BALF from 229 patients with pulmonary nodules and found differences in lung microbiota between lung cancer and benign nodules. The bacterial diversity was lower in lung cancer and four species (Porphyromonas somerae, Corynebacterium accolens, Burkholderia cenocepacia, and Streptococcus mitis) were enriched in lung cancer. Streptococcus mitis had the highest diagnostic value for differentiating lung cancer from benign lung diseases. The abundance of lung microbiota varied with different pathological subtypes of lung adenocarcinoma.
Article
Biochemistry & Molecular Biology
Enxhi Shaba, Claudia Landi, Alfonso Carleo, Lorenza Vantaggiato, Eugenio Paccagnini, Mariangela Gentile, Laura Bianchi, Pietro Lupetti, Elena Bargagli, Antje Prasse, Luca Bini
Summary: This study is the first to investigate the proteomics of EVs isolated from BALF of IPF patients, revealing that EV proteins are predominantly involved in cytoskeleton remodeling, signaling pathways, and lipid metabolism. These findings suggest a wider system involvement in the pathogenesis of IPF.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Gagandeep Kaur, Krishna Prahlad Maremanda, Michael Campos, Hitendra S. Chand, Feng Li, Nikhil Hirani, M. A. Haseeb, Dongmei Li, Irfan Rahman
Summary: The study investigated the miRNA profiles of lung-tissue-derived exosomes in COPD and IPF patients, revealing miRNA downregulation in exosomes derived from the lung tissue of COPD patients, while a large number of differentially expressed miRNAs were found in exosomes derived from the lung tissue of IPF patients.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Marina P. Sutunkova, Svetlana V. Klinova, Yuliya V. Ryabova, Anastasiya V. Tazhigulova, Ilzira A. Minigalieva, Lada V. Shabardina, Svetlana N. Solovyeva, Tatiana V. Bushueva, Larisa I. Privalova
Summary: Industrial production generates complex aerosols with ultrafine particles, which are dangerous components of inhaled air. This study investigated the cytotoxicity of different nanoparticles based on their chemical nature and dose. Tests on rats exposed to nanoparticles of CuO, PbO, CdO, Fe2O3, NiO, SiO2, Mn3O4, and SeO were conducted, and it was found that cytotoxicity varied depending on the chemical nature and dose of the nanoparticles. Changes in cytological and biochemical parameters of bronchoalveolar lavage fluid were observed, indicating the cytotoxic effects of nanoparticles. These findings suggest that cytological and biochemical analysis can be used to predict the potential danger of new nanomaterials.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Microbiology
Xiao Jin, Juan Li, Mingyue Shao, Xuedong Lv, Ningfei Ji, Yehan Zhu, Mao Huang, Feichao Yu, Changwen Zhang, Lixu Xie, Jianling Huang, Sixi Chen, Changjun Zhu, Minjie Lv, Ganzhu Feng
Summary: mNGS has shown superior performance in the diagnosis of suspected pulmonary infection, especially in detecting Mycobacterium tuberculosis, atypical pathogens, viruses, and fungi. It significantly improves the diagnosis and guides targeted treatment and clinical management. The unbiased nature of mNGS makes it a promising microbiological detection and diagnostic method in clinical practice.
MICROBIOLOGY SPECTRUM
(2022)
Review
Cell Biology
Ziyu Liu, Jiaqing Yan, Lingling Tong, Shouyue Liu, Ying Zhang
Summary: Exosomes, released from a variety of cells, have been shown to effectively deliver various molecules to target cells or tissues. Exosomes from bronchoalveolar lavage fluid play a crucial role in intercellular communication and diagnosis of pulmonary diseases.
JOURNAL OF CELLULAR PHYSIOLOGY
(2022)
Article
Medicine, General & Internal
Hanying Liu, Ying Zhang, Guiyang Chen, Shenghua Sun, Jiangang Wang, Fengyi Chen, Chun Liu, Quan Zhuang
Summary: mNGS can detect pathogens in a more timely and accurate manner, assisting clinicians in adjusting antibiotics in time. Therefore, we recommend mNGS as a complementary diagnostic tool for severe pneumonia or complicated infections.
FRONTIERS IN MEDICINE
(2022)
Article
Engineering, Biomedical
Yafeng Li, Xiaoxiang Liao, Bin Zheng
Summary: This study developed photo-triggered implantable polymeric microneedles for drug delivery in a transdermal analgesia system. The prepared PCL/PVP-Fe(3)O(4)NPs were spherical in shape, with sizes ranging from 9 to 11 nm, and showed outstanding insertion ability in porcine skin. Additionally, near-infrared (NIR) irradiation could enhance drug delivery, allowing for painless delivery of lidocaine hydrochloride through the stratum corneum barrier, with a quick onset time.
JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION
(2022)
Article
Chemistry, Inorganic & Nuclear
A. S. Kubasov, E. S. Turishev, A. Kopytin, L. K. Shpigun, K. Yu Zhizhin, N. T. Kuznetsov
Summary: A method for preparing sulfonium derivatives of closo-hydridodecaborate anions with exo-polyhedral alkyl groups has been developed, along with a procedure for preparing mono-S-substituted derivatives through interaction with secondary alkyl halides. The sulfonium derivative has been studied as an active component for a lidocaine-selective sensor, with optimal membrane composition identified and a detection limit of 3.5 . 10(-8) M determined.
INORGANICA CHIMICA ACTA
(2021)
Article
Microbiology
Daniele Roberto Giacobbe, Juergen Prattes, Joost Wauters, Silvia Dettori, Alessio Signori, Jon Salmanton-Garcia, Johan Maertens, Marc Bourgeois, Marijke Reynders, Lynn Rutsaert, Niels Van Regenmortel, Piet Lormans, Simon Feys, Nikolay Klimko, Olga Shadrivova, Oliver A. Cornely, Riina Rautemaa-Richardson, Philipp Koehler, Katrien Lagrou, Matteo Bassetti, Martin Hoenigl
Summary: Critically ill patients with COVID-19 may develop COVID-19-associated pulmonary aspergillosis (CAPA), and positive bronchoalveolar lavage fluid (BALF) mycological tests are associated with increased 90-day mortality.
JOURNAL OF CLINICAL MICROBIOLOGY
(2022)
Article
Dentistry, Oral Surgery & Medicine
Hakan Arslan, Ezgi Doganay Yildiz, Huseyin Sinan Topcuoglu, Ebru Tepecik, Gizem Tas
Summary: The study found that calcium hydroxide mixed with lidocaine HCl can significantly reduce postoperative pain in patients with irreversible pulpitis and symptomatic apical periodontitis. The mixture was more effective than calcium hydroxide mixed with saline in reducing pain during days 1 to 4 postoperatively.
CLINICAL ORAL INVESTIGATIONS
(2021)