Article
Chemistry, Physical
Karlo Guerrero, Roberto Gallardo, Ivan Paredes, Julian Quintero, Silvia Mau, Raul Conejeros, Juan Carlos Gentina, German Aroca
Summary: The degenerated strain of Clostridium acetobutylicum obtained through continuous batch cultures showed potential for high hydrogen production in continuous culture, achieving good hydrogen yield and stability, providing an interesting alternative for hydrogen production.
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
(2021)
Article
Biotechnology & Applied Microbiology
Sang Hyun Kim, Jeong Hyeon Hwang, Hyun Joong Kim, Suk Jin Oh, Hyun Jin Kim, Nara Shin, Sang-Hyoun Kim, Jeong-Hoon Park, Shashi Kant Bhatia, Yung-Hun Yang
Summary: The study developed Clostridium acetobutylicum as a superior biohydrogen producer by introducing glyceraldehyde-3-phosphate dehydrogenase (GAPDH) to enhance hydrogen production. The strain CAC824-G containing gapC showed a 66.3% higher hydrogen production than the wild-type strain, with increased NADH and NADPH pools. Overexpression of gapC resulted in increased hydrogen production even in the presence of inhibitors.
ENZYME AND MICROBIAL TECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Karlo Guerrero, Roberto Gallardo, Ernesto Gonzalez, Fabian Veliz, Raul Conejeros, Juan Carlos Gentina, German Aroca
Summary: By adding butyrate and neutral red to the culture medium, and using electro-fermentation with an external electron source, the production of butanol can be increased. However, the presence of butyrate and the absence of neutral red in electro-fermentation are detrimental to the microorganism.
JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Zongjie Dai, Yan Zhu, Hongjun Dong, Chunhua Zhao, Yanping Zhang, Yin Li
Summary: Depletion of intracellular ATP significantly increased glycolytic rate, enhanced solvent production, and resulted in a wide range of metabolic changes in C. acetobutylicum DSM 1731. This finding provides a novel strategy for engineering solvent-producing C. acetobutylicum and other anaerobic microbial cell factories.
MICROBIAL CELL FACTORIES
(2021)
Review
Biotechnology & Applied Microbiology
Petra Patakova, Barbora Branska, Maryna Vasylkivska, Katerina Jureckova, Jana Musilova, Ivo Provaznik, Karel Sedlar
Summary: Solventogenic clostridia, although not a strictly defined group within the genus Clostridium, share common features such as being anaerobic, non-pathogenic, non-toxinogenic, and endospore forming bacteria. Their main metabolite is typically 1-butanol, but they can also produce other metabolites depending on species and culture conditions. Despite being used for industrial production in the past, they were replaced by more efficient chemical production methods. Transcriptome analysis is a valuable tool for gaining deeper insights into clostridial metabolism and life.
BIOTECHNOLOGY ADVANCES
(2022)
Article
Materials Science, Paper & Wood
Basirah Fauzi, Mohd Ghazali Mohd Nawawi, Roslinda Fauzi, Nurul Izzati Mohd Ismail, Siti Fatimah Mohd Noor, Izat Yahaya, Syazwan Hanani Meriam Suhaimy, Muhammad Sufi Roslan
Summary: A fermentation medium for the synthesis of butanol was designed, and it was found that the optimal glucose concentration for maximum growth and butanol production of Clostridium acetobutylicum was 60 g/L. Further increase in glucose content would deteriorate the performance of butanol production.
Article
Engineering, Environmental
Marwa M. El-Dalatony, Bikram Basak, Mayur B. Kurade, Hyun-Seog Roh, Min Jang, Byong-Hun Jeon
Summary: This study demonstrates the effective production of biohydrogen (bioH(2)) and ABE through ABE fermentation using sonication pretreated Chlamydomonas mexicana biomass as a cost-effective substrate. Sonication enhances the recovery and bioaccessibility of carbohydrates and proteins, resulting in higher yields of bioH(2) and volatile fatty acids. The study shows the efficient conversion of soluble carbohydrates and proteins into bioH(2) and ABE, which can be used for high-energy biofuel production with minimal waste generation.
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
(2022)
Article
Biotechnology & Applied Microbiology
M. Capilla, P. San-Valero, M. Izquierdo, J. M. Penya-roja, C. Gabaldon
Summary: This study evaluated the influence of initial glucose concentrations and pH control strategies on biobutanol production and glucose consumption. The results showed that different pH control conditions had a significant impact on butanol production under different initial glucose concentrations.
BIOCHEMICAL ENGINEERING JOURNAL
(2021)
Article
Energy & Fuels
Zhiyou Zhou, Yujie Jing, Saijin Wei, Qinghua Zhang, Shuaiying Peng, Xuejiao An, Hanguang Li
Summary: In this study, an exogenous regulatory strategy named oxidation-reduction potential (ORP) regulation was used to enhance butanol production. The findings showed that controlling the ORP value at -400 mV resulted in the highest butanol production, with increased yield and productivity. The study also investigated the physiological mechanisms of ORP regulation on butanol synthesis.
Article
Microbiology
Jonathan R. Humphreys, Zak Bean, Jamie Twycross, Klaus Winzer
Summary: Lanthipeptide synthetases, present in all domains of life, catalyze an important step in lanthipeptide biosynthesis. LanM-like proteins, found in Clostridium species, are associated with agr quorum sensing operons. The physiological role and mode of action of these proteins are unclear. In this study, the LanM-like protein CA_C0082 was shown to act as an effector of Agr quorum sensing, controlling granulose formation and spore and solvent production in Clostridium acetobutylicum.
Article
Biochemical Research Methods
Sireethorn Khaonuan, Rattana Jariyaboon, Nikannapas Usmanbaha, Benjamas Cheirsilp, Nils-Kare Birkeland, Prawit Kongjan
Summary: Marine macroalgae, specifically Gracilaria tenuistipitata, showed the highest potential for butanol production through acetone-butanol-ethanol fermentation. Low-temperature hydrothermal pretreatment of G. tenuistipitata resulted in a high reducing sugar yield rate and ABE yield.
BIOTECHNOLOGY JOURNAL
(2023)
Article
Agricultural Engineering
Piyawat Chinwatpaiboon, Ancharida Savarajara, Apanee Luengnaruemitchai
Summary: This study aimed to investigate the utilization of water hyacinth as a feedstock for biofuel production through acetone-butanol-ethanol fermentation. The water hyacinth was pretreated with sodium hydroxide at different concentrations, reaction times, and temperatures. The optimal pretreatment condition was found to be 2% (w/v) NaOH for 90 minutes at 100 degrees C, resulting in a maximum total sugar concentration of 21.05 g/L. The subsequent fermentation by C. beijerinckii JCM 8026 yielded a butanol concentration, yield, and productivity of 2.09 g/L, 0.15 g/g, and 0.012 g/L/h, respectively.
BIOMASS & BIOENERGY
(2023)
Article
Chemistry, Physical
Abdullah Bilal Ozturk, Tulin Arasoglu, Jale Gulen, Shou Cheng, Najeeb Kaid Nasser Al-Shorgani, Hiroaki Habaki, Ryuichi Egashira, Mohd Sahaid Kalil, Wan Mohtar Wan Yusoff, Jeffrey Scott Cross
Summary: The study focuses on utilizing cooked rice waste for biobutanol production, conducting techno-economic evaluation and process optimization through process simulation tools, resulting in cost estimation and improvement measures.
SUSTAINABLE ENERGY & FUELS
(2021)
Article
Thermodynamics
Zhiyou Zhou, Shuaiying Peng, Yujie Jing, Saijin Wei, Qinghua Zhang, Huanhuan Ding, Hanguang Li
Summary: An efficient pretreatment technology, known as DALP, was developed for the bioconversion of puerariae slag into biobutanol. The method of dilute acid combined with laccase pretreatment significantly accelerated the enzymatic saccharification of puerariae slag. The highest concentration of reducing sugar obtained was 48.96 ± 1.69 g/L, and the highest butanol productivity achieved was 0.15 g/L/h.
