Article
Fisheries
Xiaohong Huang, Jingguang Wei, Qi Zheng, Ya Zhang, Weibin Zhu, Jiangchun Liu, Yunxia Hou, Qiwei Qin, Youhua Huang
Summary: The spotted knifejaw (Oplegnathus punctatus) is an emerging economical aquaculture species in China. In this study, an iridovirus-like disease outbreak (SKIV-SD) in the spotted knifejaw aquaculture industry in Shandong Province was characterized. The SKIV-SD virus, identified as an ISKNV-type megalocytivirus, caused severe mortality and pathological lesions in cultured spotted knifejaw, sharing genetic similarities with infectious spleen and kidney necrosis virus (ISKNV).
Article
Fisheries
Hanchang Sohn, Seongdo Lee, Hyukjae Kwon, Mun Gyeong Kwon, Jee Youn Hwang, Seong Don Hwang, Jehee Lee
Summary: A multiplex PCR method was established to detect two genotypes of Megalocytivirus, RSIV and TRBIV, with optimized primer pairs for increased sensitivity and specificity. The evaluation showed successful differentiation of 21 Korean Megalocytivirus isolates, including 20 RSIV and one TRBIV, providing a convenient tool for diagnosing viral diseases in the Korean aquaculture industry.
AQUACULTURE REPORTS
(2021)
Article
Fisheries
Wenji Wang, Mengke Liu, Weihua Xu, Shiting Chen, Qiwei Qin, Xiaohong Huang, Youhua Huang
Summary: Increasing evidence has shown that megalocytiviruses cause severe disease with high mortality in freshwater and marine species. However, the molecular events involved in megalocytivirus infection in vitro are still not well understood. This study investigated the transcriptomic profiles of sea perch fry (SPF) cells infected with sea perch iridovirus (SPIV) in vitro and examined the roles of crucial molecular events during infection. The results demonstrated that SPIV infection induces DNA damage response (DDR) and cell cycle arrest, with the ATR-CHK1 pathway playing a crucial role in viral replication. These findings provide new insights into the interaction between megalocytiviruses and host cells in vitro.
Article
Fisheries
Myung-Hwa Jung, Sajal Kole, Sung-Ju Jung
Summary: This study aims to develop a safe and efficient inactivated (IV) vaccine against rock bream iridovirus (RBIV). The evaluation results show that saponin (Sa) acts as an adjuvant to induce antiviral immune response and protect rock bream from RBIV infection. The Sa-adjuvanted IV vaccine significantly improves fish survivability through upregulation of IL1 beta, Mx, and PKR gene transcript. It provides strong protection against reinfection with RBIV.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Fisheries
Hsiang-Chieh Chuang, Tah-Wei Chu, Ann-Chang Cheng, Nai-Yu Chen, Yu-Shen Lai
Summary: Ornamental fish are often infected with iridoviruses, which can easily spread through international trade. The study showed that iridoviruses can be infectious in both freshwater and marine water habitats, resulting in high mortality rates in infected fish. The mortality rate of marine fish infected with iridoviruses was higher than that of freshwater fish.
Article
Fisheries
Channapha Sakseepipad, Reiko Nozaki, Keisuke Yoshii, Yutaka Fukuda, Kaori Mizuno, Hidemasa Kawakami, Ikuo Hirono, Hidehiro Kondo
Summary: This study developed two primer sets to amplify SNP regions of RSIV-type megalocytiviruses, identifying 53 polymorphic sites and clustering 25 isolates into eight haplotypes through phylogenetic analysis. These findings suggest that SNPs in the RSIV genome are a powerful tool for detection and identification of RSIV-type megalocytiviruses.
JOURNAL OF FISH DISEASES
(2021)
Article
Biochemistry & Molecular Biology
Yu Pan, Xiang Zhao, Di Li, Tianxiang Gao, Na Song
Summary: Transcriptome analysis of Banggai cardinalfish revealed significant gene expression changes in response to different temperatures, identifying 47 genes potentially related to temperature stress. This study provides valuable information for further research on temperature tolerance in Banggai cardinalfish.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS
(2021)
Article
Fisheries
Zhenxing Liu, Yanping Ma, Le Hao
Summary: Although several cell lines derived from fish brain tissue have been constructed, little is known about their specific cell types. Three new cell lines were established from spotted sea bass brain tissue, with one identified as astroglia-like cells, one showing smooth muscle-like features, and the third exhibiting activation of the WNT/beta-catenin pathway. Apart from differences in cell marker expression profiles, these cell lines also showed variations in transfection efficiency and susceptibility to iridovirus infection. The study also led to the discovery of a novel megalocytivirus from these cell lines, providing insights into cell-virus interactions.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Multidisciplinary Sciences
Samantha A. A. Koda, Kuttichantran Subramaniam, Paul M. M. Hick, Evelyn Hall, Thomas B. B. Waltzek, Joy A. A. Becker
Summary: A newly developed TaqMan qPCR assay was able to detect different genotypes of ISKNV with high sensitivity and specificity. This assay can be used for surveillance and diagnosis of ISKNV in the aquaculture and ornamental fish industries.
Article
Fisheries
Kyung-Ho Kim, Kwang-Min Choi, Gyoungsik Kang, Won-Sik Woo, Min-Young Sohn, Ha-Jeong Son, Dongbin Yun, Do-Hyung Kim, Chan-Il Park
Summary: The diagnostic performance of a TaqMan-based real-time PCR assay for detecting red sea bream iridovirus (RSIV) was evaluated. The assay showed rapid and accurate detection of RSIV, with high sensitivity and specificity.
Article
Biochemistry & Molecular Biology
Krisztina Nikovics, Cedric Castellarin, Xavier Holy, Marjorie Durand, Halima Morin, Abdelhafid Bendahmane, Anne-Laure Favier
Summary: Understanding bone homeostasis and regeneration is of great clinical and scientific importance for the development of bone therapy. Macrophages play a crucial role in bone regeneration during healing. In situ hybridization and hybridization chain reaction can be used to explore gene expression in bone tissues.
Review
Biochemistry & Molecular Biology
Krisztina Nikovics, Anne-Laure Favier
Summary: Understanding the pivotal role of macrophages in inflammation and tissue regeneration post-injury is crucial. Recent advancements in macrophage biology studies have revealed distinctive features of in vivo macrophages, emphasizing the importance of precise characterization for developing new healing treatments. Improvements in in situ macrophage identification using antigen surface markers and cytokine secretion identification have led to specific patterns in macrophage characterization.
Article
Materials Science, Multidisciplinary
Yeguang Wang, Chengjun Liu
Summary: Novel experiments using confocal laser scanning microscopy were conducted to observe the transient evolution of inclusions morphology and size during the modification process from Al2O3 and MgO-Al2O3 to low-melting point CaO-Al2O3-(MgO). The experiments detected the disaggregation of large-sized Al2O3 clusters by Ca treatment, resulting in the reduction of inclusions size in melts.
METALLURGICAL AND MATERIALS TRANSACTIONS B-PROCESS METALLURGY AND MATERIALS PROCESSING SCIENCE
(2022)
Article
Microbiology
Weixuan Fu, Yong Li, Yuting Fu, Wenfeng Zhang, Panpan Luo, Qianqian Sun, Fangzhao Yu, Shaoping Weng, Wangdong Li, Jianguo He, Chuanfu Dong
Summary: Red seabream iridovirus (RSIV) is a major cause of economic loss in mariculture bony fish worldwide. Previous studies have shown that the diversity of RSIV isolates can affect their virulence, antigenicity, and vaccine efficacy. This study demonstrated that ISKNV-I-based inactivated vaccine can provide almost complete cross-protection against RSIV-I and RSIV-II, as well as ISKNV-I itself.
MICROBIOLOGY SPECTRUM
(2023)
Article
Microbiology
Yasuhiko Kawato, Tohru Mekata, Mari Inada, Takafumi Ito
Summary: Red sea bream iridoviral disease (RSIVD) causes significant economic damage in mariculture in Asian countries. The study monitored the dynamics of RSIV in a fish farm over 3 years and found that outbreaks in juveniles originated from asymptomatically virus-infected broodstocks, highlighting the potential of using eDNA monitoring as a tool for controlling fish diseases in aquaculture.
MICROBIOLOGY SPECTRUM
(2021)
Editorial Material
Biotechnology & Applied Microbiology
V. Gregory Chinchar, Paul Hick, Ikbal Agah Ince, James K. Jancovich, Rachel Marschang, Qiwei Qin, Kuttichantran Subramaniam, Thomas B. Waltzek, Richard Whittington, Trevor Williams, Qi-Ya Zhang
JOURNAL OF GENERAL VIROLOGY
(2017)
Article
Veterinary Sciences
Bryan S. Vorbach, David S. Rotstein, Nicole I. Stacy, Carla Mavian, Marco Salemi, Thomas B. Waltzek, Martine de Wit
JOURNAL OF WILDLIFE DISEASES
(2017)
Article
Virology
V. Gregory Chinchar, Thomas B. Waltzek, Kuttichantran Subramaniam
Article
Virology
Paul M. Hick, Kuttichantran Subramaniam, Patrick M. Thompson, Thomas B. Waltzek, Joy A. Becker, Richard J. Whittington
Article
Virology
Sieara C. Claytor, Kuttichantran Subramaniam, Nelmarie Landrau-Giovannetti, V. Gregory Chinchar, Matthew J. Gray, Debra L. Miller, Carla Mavian, Marco Salemi, Samantha Wisely, Thomas B. Waltzek
Article
Microbiology
Md. Shamim Ahasan, Thomas B. Waltzek, Roger Huerlimann, Ellen Ariel
MICROBIOLOGICAL RESEARCH
(2018)
Article
Virology
Sunil Kumar Mor, Nicholas B. D. Phelps, Terry Fei Fan Ng, Kuttichantran Subramaniam, Alexander Primus, Anibal G. Armien, Rebekah McCann, Corey Puzach, Thomas B. Waltzek, Sagar M. Goyal
ARCHIVES OF VIROLOGY
(2017)
Article
Microbiology
Tania S. Bonny, Kuttichantran Subramaniam, Thomas B. Waltzek, Maha A. Elbadry, Valery Madsen Beau De Rochars, Taina Telisma, Mohammed Rashid, J. Glenn Morris, John A. Lednicky
GENOME ANNOUNCEMENTS
(2017)
Article
Microbiology
Andrew J. Davison, Ole Nielsen, Kuttichantran Subramaniam, Jessica M. Jacob, Carlos H. Romero, Kathy A. Burek-Huntington, Thomas B. Waltzek
GENOME ANNOUNCEMENTS
(2017)
Article
Microbiology
Kuttichantran Subramaniam, John A. Lednicky, Julia Loeb, Katherine A. Sayler, Samantha M. Wisely, Thomas B. Waltzek
GENOME ANNOUNCEMENTS
(2017)
Article
Microbiology
Andrew J. Davison, Kuttichantran Subramaniam, Karen Kerr, Jessica M. Jacob, Nelmarie Landrau-Giovannetti, Michael T. Walsh, Randall S. Wells, Thomas B. Waltzek
GENOME ANNOUNCEMENTS
(2017)
Article
Fisheries
Esteban Soto, Christine Richey, Stephen R. Reichley, Brittany Stevens, Kirsten V. Kenelty, Janiee Lewis, Barbara Byrne, Nathan P. Wiederhold, Thomas B. Waltzek, Matthew F. Sheley, Alvin C. Camus, Matt J. Griffin
DISEASES OF AQUATIC ORGANISMS
(2017)
Article
Virology
Steven J. van Beurden, Lonneke L. IJsseldijk, Marco W. G. van de Bildt, Lineke Begeman, James F. X. Wellehan, Thomas B. Waltzek, Geert de Vrieze, Andrea Grone, Thijs Kuiken, M. Helene Verheije, Judit J. Penzes
ARCHIVES OF VIROLOGY
(2017)
Article
Fisheries
Pedro H. O. Viadanna, Tim Miller-Morgan, Trace Peterson, Keith Way, David M. Stone, Gary D. Marty, Fabiana Pilarski, Ronald P. Hedrick, Thomas B. Waltzek
DISEASES OF AQUATIC ORGANISMS
(2017)
Article
Veterinary Sciences
Galaxia Cortes-Hinojosa, Bethany Doescher, Michael Kinsel, John Lednicky, Julia Loeb, Thomas Waltzek, James F. X. Wellehan
JOURNAL OF ZOO AND WILDLIFE MEDICINE
(2016)