Alleviation of off-target effects from vector-encoded shRNAs via codelivered RNA decoys

miR-122 – A key factor and therapeutic target in liver disease

(2015) Simonetta Bandiera et al.   JOURNAL OF HEPATOLOGY

In VitroAntiviral Activity and Preclinical and Clinical Resistance Profile of Miravirsen, a Novel Anti-Hepatitis C Virus Therapeutic Targeting the Human Factor miR-122

(2014) Søren Ottosen et al.   ANTIMICROBIAL AGENTS AND CHEMOTHERAPY

Development and Applications of CRISPR-Cas9 for Genome Engineering

(2014) Patrick D. Hsu et al.   CELL

Pegylated interferon based therapy with second-wave direct-acting antivirals in genotype 1 chronic hepatitis C

(2014) Nicole M. Welch et al.   LIVER INTERNATIONAL

A Systematic Mammalian Genetic Interaction Map Reveals Pathways Underlying Ricin Susceptibility

(2013) Michael C. Bassik et al.   CELL

Formulated Minimal-Length Synthetic Small Hairpin RNAs Are Potent Inhibitors of Hepatitis C Virus in Mice With Humanized Livers

(2013) Han Ma et al.   GASTROENTEROLOGY

Molecular dissection of human Argonaute proteins by DNA shuffling

(2013) Nina Schürmann et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Treatment of HCV Infection by Targeting MicroRNA

(2013) Harry L.A. Janssen et al.   NEW ENGLAND JOURNAL OF MEDICINE

The anti-genomic (negative) strand of Hepatitis C Virus is not targetable by shRNA

(2013) Leszek Lisowski et al.   NUCLEIC ACIDS RESEARCH

Dicer-independent processing of short hairpin RNAs

(2013) Y. P. Liu et al.   NUCLEIC ACIDS RESEARCH

Robust RNAi enhancement via human Argonaute-2 overexpression from plasmids, viral vectors and cell lines

(2013) Kathleen Börner et al.   NUCLEIC ACIDS RESEARCH

Integrated platform for genome-wide screening and construction of high-density genetic interaction maps in mammalian cells

(2013) M. Kampmann et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Argonaute-3 activates the let-7a passenger strand microRNA

(2013) Julia Winter et al.   RNA Biology

Suppression of microRNAs by dual-targeting and clustered Tough Decoy inhibitors

(2013) Anne Kruse Hollensen et al.   RNA Biology

Multiple sensors ensure guide strand selection in human RNAi pathways

(2013) C. L. Noland et al.   RNA

Prospects for nucleic acid-based therapeutics against hepatitis C virus

(2013) Chang Ho Lee   WORLD JOURNAL OF GASTROENTEROLOGY

Adenovirus vectors lacking virus-associated RNA expression enhance shRNA activity to suppress hepatitis C virus replication

(2013) Zheng Pei et al.   Scientific Reports

The Loop Position of shRNAs and Pre-miRNAs Is Critical for the Accuracy of Dicer Processing In Vivo

(2012) Shuo Gu et al.   CELL

A genome-wide homologous recombination screen identifies the RNA-binding protein RBMX as a component of the DNA-damage response

(2012) Britt Adamson et al.   NATURE CELL BIOLOGY

Long-term, efficient inhibition of microRNA function in mice using rAAV vectors

(2012) Jun Xie et al.   NATURE METHODS

The N domain of Argonaute drives duplex unwinding during RISC assembly

(2012) Pieter Bas Kwak et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

A potent 2′ -O- methylated RNA-based microRNA inhibitor with unique secondary structures

(2012) Takeshi Haraguchi et al.   NUCLEIC ACIDS RESEARCH

Right- and left-loop short shRNAs have distinct and unusual mechanisms of gene silencing

(2012) Anne Dallas et al.   NUCLEIC ACIDS RESEARCH

Functional parameters of Dicer-independent microRNA biogenesis

(2012) J.-S. Yang et al.   RNA

Potent microRNA suppression by RNA Pol II-transcribed 'Tough Decoy' inhibitors

(2012) R. O. Bak et al.   RNA

The effects of stem length and core placement on shRNA activity

(2011) Glen J Mcintyre et al.   BMC MOLECULAR BIOLOGY

New targets for antiviral therapy of chronic hepatitis C

(2011) Sandra Bühler et al.   LIVER INTERNATIONAL

siRNA Repositioning for Guide Strand Selection by Human Dicer Complexes

(2011) Cameron L. Noland et al.   MOLECULAR CELL

Functional Identification of Optimized RNAi Triggers Using a Massively Parallel Sensor Assay

(2011) Christof Fellmann et al.   MOLECULAR CELL

Rational Design of Therapeutic siRNAs: Minimizing Off-targeting Potential to Improve the Safety of RNAi Therapy for Huntington's Disease

(2011) Ryan L Boudreau et al.   MOLECULAR THERAPY

Thermodynamic stability of small hairpin RNAs highly influences the loading process of different mammalian Argonautes

(2011) S. Gu et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Argonaute proteins regulate microRNA stability: Increased microRNA abundance by Argonaute proteins is due to microRNA stabilization

(2011) Julia Winter et al.   RNA Biology

Dicer is dispensable for asymmetric RISC loading in mammals

(2011) J. G. Betancur et al.   RNA

Argonaute proteins are key determinants of RNAi efficacy, toxicity, and persistence in the adult mouse liver

(2010) Dirk Grimm et al.   JOURNAL OF CLINICAL INVESTIGATION

Conserved vertebrate mir-451 provides a platform for Dicer-independent, Ago2-mediated microRNA biogenesis

(2010) J.-S. Yang et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A Novel miRNA Processing Pathway Independent of Dicer Requires Argonaute2 Catalytic Activity

(2010) D. Cifuentes et al.   SCIENCE

Asymmetry in siRNA design

(2009) D Grimm   GENE THERAPY

Low-level shRNA Cytotoxicity Can Contribute to MYC-induced Hepatocellular Carcinoma in Adult Mice

(2009) Shelly Beer et al.   MOLECULAR THERAPY

The promises and pitfalls of RNA-interference-based therapeutics

(2009) Daniela Castanotto et al.   NATURE

Rapid creation and quantitative monitoring of high coverage shRNA libraries

(2009) Michael C Bassik et al.   NATURE METHODS

Recognizing and avoiding siRNA off-target effects for target identification and therapeutic application

(2009) Aimee L. Jackson et al.   NATURE REVIEWS DRUG DISCOVERY

Off-target and a portion of target-specific siRNA mediated mRNA degradation is Ago2 ‘Slicer’ independent and can be mediated by Ago1

(2009) Timothy A. Vickers et al.   NUCLEIC ACIDS RESEARCH

Vectors expressing efficient RNA decoys achieve the long-term suppression of specific microRNA activity in mammalian cells

(2009) Takeshi Haraguchi et al.   NUCLEIC ACIDS RESEARCH

Therapeutic Silencing of MicroRNA-122 in Primates with Chronic Hepatitis C Virus Infection

(2009) R. E. Lanford et al.   SCIENCE

In Vitro and In Vivo Gene Therapy Vector Evolution via Multispecies Interbreeding and Retargeting of Adeno-Associated Viruses

(2008) D. Grimm et al.   JOURNAL OF VIROLOGY

Expression of shRNA From a Tissue-specific pol II Promoter Is an Effective and Safe RNAi Therapeutic

(2008) Jeffery C Giering et al.   MOLECULAR THERAPY

Artificial MicroRNAs as siRNA Shuttles: Improved Safety as Compared to shRNAs In vitro and In vivo

(2008) Ryan L Boudreau et al.   MOLECULAR THERAPY

The regulatory activity of microRNA* species has substantial influence on microRNA and 3′ UTR evolution

(2008) Katsutomo Okamura et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Minimizing variables among hairpin-based RNAi vectors reveals the potency of shRNAs

(2008) R. L. Boudreau et al.   RNA