Dissecting enzyme function with microfluidic-based deep mutational scanning
Published 2015 View Full Article
- Home
- Publications
- Publication Search
- Publication Details
Title
Dissecting enzyme function with microfluidic-based deep mutational scanning
Authors
Keywords
-
Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 112, Issue 23, Pages 7159-7164
Publisher
Proceedings of the National Academy of Sciences
Online
2015-05-27
DOI
10.1073/pnas.1422285112
References
Ask authors/readers for more resources
Related references
Note: Only part of the references are listed.- Accurate microfluidic sorting of droplets at 30 kHz
- (2015) Adam Sciambi et al. LAB ON A CHIP
- Using droplet-based microfluidics to improve the catalytic properties of RNA under multiple-turnover conditions
- (2015) Michael Ryckelynck et al. RNA
- CotA laccase: high-throughput manipulation and analysis of recombinant enzyme libraries expressed in E. coli using droplet-based microfluidics
- (2014) Thomas Beneyton et al. ANALYST
- Deep mutational scanning: a new style of protein science
- (2014) Douglas M Fowler et al. NATURE METHODS
- An Improved Single Cell Ultrahigh Throughput Screening Method Based on In Vitro Compartmentalization
- (2014) Fuqiang Ma et al. PLoS One
- Ultrahigh-throughput sorting of microfluidic drops with flow cytometry
- (2013) Shaun W. Lim et al. LAB ON A CHIP
- The carbohydrate-active enzymes database (CAZy) in 2013
- (2013) Vincent Lombard et al. NUCLEIC ACIDS RESEARCH
- Resistance to vemurafenib resulting from a novel mutation in the BRAFV600E kinase domain
- (2013) Timothy R. Wagenaar et al. Pigment Cell & Melanoma Research
- Navigating the protein fitness landscape with Gaussian processes
- (2013) P. A. Romero et al. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
- Capturing the mutational landscape of the beta-lactamase TEM-1
- (2013) H. Jacquier et al. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
- Hierarchical molecular tagging to resolve long continuous sequences by massively parallel sequencing
- (2013) Sverker Lundin et al. Scientific Reports
- Picoliter Cell Lysate Assays in Microfluidic Droplet Compartments for Directed Enzyme Evolution
- (2012) Balint Kintses et al. CHEMISTRY & BIOLOGY
- Stabilizing Proteins from Sequence Statistics: The Interplay of Conservation and Correlation in Triosephosphate Isomerase Stability
- (2012) Brandon J. Sullivan et al. JOURNAL OF MOLECULAR BIOLOGY
- Systematic Identification of H274Y Compensatory Mutations in Influenza A Virus Neuraminidase by High-Throughput Screening
- (2012) N. C. Wu et al. JOURNAL OF VIROLOGY
- A completely in vitro ultrahigh-throughput droplet-based microfluidic screening system for protein engineering and directed evolution
- (2012) Ali Fallah-Araghi et al. LAB ON A CHIP
- The spatial architecture of protein function and adaptation
- (2012) Richard N. McLaughlin Jr et al. NATURE
- Optimization of affinity, specificity and function of designed influenza inhibitors using deep sequencing
- (2012) Timothy A Whitehead et al. NATURE BIOTECHNOLOGY
- Fast gapped-read alignment with Bowtie 2
- (2012) Ben Langmead et al. NATURE METHODS
- A fundamental protein property, thermodynamic stability, revealed solely from large-scale measurements of protein function
- (2012) C. L. Araya et al. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
- Dynamics of molecular transport by surfactants in emulsions
- (2012) Yousr Skhiri et al. Soft Matter
- Protein Model Discrimination Using Mutational Sensitivity Derived from Deep Sequencing
- (2012) Bharat V. Adkar et al. STRUCTURE
- Circular polymerase extension cloning for high-throughput cloning of complex and combinatorial DNA libraries
- (2011) Jiayuan Quan et al. Nature Protocols
- Experimental illumination of a fitness landscape
- (2011) R. T. Hietpas et al. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
- High-Throughput Screening of Enzymes by Retroviral Display Using Droplet-Based Microfluidics
- (2010) Lucia Granieri et al. CHEMISTRY & BIOLOGY
- Parallel, tag-directed assembly of locally derived short sequence reads
- (2010) Joseph B Hiatt et al. NATURE METHODS
- High-resolution mapping of protein sequence-function relationships
- (2010) Douglas M Fowler et al. NATURE METHODS
- Exploring challenges in rational enzyme design by simulating the catalysis in artificial kemp eliminase
- (2010) M. P. Frushicheva et al. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
- Origins of catalysis by computationally designed retroaldolase enzymes
- (2010) J. K. Lassila et al. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
- Ultrahigh-throughput screening in drop-based microfluidics for directed evolution
- (2010) J. J. Agresti et al. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
- An exciting but challenging road ahead for computational enzyme design
- (2010) David Baker PROTEIN SCIENCE
- Protein Sectors: Evolutionary Units of Three-Dimensional Structure
- (2009) Najeeb Halabi et al. CELL
- From Microdroplets to Microfluidics: Selective Emulsion Separation in Microfluidic Devices
- (2008) Luis M. Fidalgo et al. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
- Phylogeny.fr: robust phylogenetic analysis for the non-specialist
- (2008) A. Dereeper et al. NUCLEIC ACIDS RESEARCH
Find Funding. Review Successful Grants.
Explore over 25,000 new funding opportunities and over 6,000,000 successful grants.
ExploreBecome a Peeref-certified reviewer
The Peeref Institute provides free reviewer training that teaches the core competencies of the academic peer review process.
Get Started