Article
Genetics & Heredity
Stanley Dean Rider Jr, French J. J. Damewood, Rujuta Yashodhan Gadgil, David C. C. Hitch, Venicia Alhawach, Resha Shrestha, Matilyn Shanahan, Nathen Zavada, Michael Leffak
Summary: Unbiased genetic screens using a lentiviral shRNA library identified genes that suppress break-induced mutagenesis in human cells with fragile non-B DNA. These genes have roles in DNA replication and repair, chromatin modification, responses to ionizing radiation, and replication fork-associated proteins. Novel loci implicated in BIR were also identified. Knockdown of selected candidates increased the frequency of nucleoside analog resistance and DNA rearrangements near the non-B DNA.
Article
Chemistry, Analytical
Qingqing Zhang, Heng Gao, Chengxiao Yan, Tong Yang, Xiong Zheng, Qiuda Xu, Dandan Wang, Xiao-Shun Zhou, Yong Shao
Summary: The study uncovers the rationale in designing ligands to selectively resolve the E-motif nucleotides within TRs, achieving E-motif selectivity through specific hydrogen bonding interactions.
ANALYTICA CHIMICA ACTA
(2022)
Review
Cell Biology
Elisa Balzano, Franca Pelliccia, Simona Giunta
Summary: Repeated sequences make up more than half of the human genome and play a significant role in various physiological and pathological conditions. Tandem repeats, a major category of repetitive DNA in the human genome, have a higher mutation rate and are prone to aberrant recombination, leading to associations with a variety of diseases.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Yirong Shi, Yiwei Niu, Peng Zhang, Huaxia Luo, Shuai Liu, Sijia Zhang, Jiajia Wang, Yanyan Li, Xinyue Liu, Tingrui Song, Tao Xu, Shunmin He
Summary: This study presents a comprehensive map of 366,013 polymorphic short tandem repeats (pSTRs) in the human genome, based on deeply sequenced genomes. The study explores the associations between STR mutations and various genomic features, such as gene expression and alternative polyadenylation. It also investigates population differentiation and genotypes disease-causing STRs.
NATURE COMMUNICATIONS
(2023)
Article
Multidisciplinary Sciences
Corella S. Casas-Delucchi, Manuel Daza-Martin, Sophie L. Williams, Gideon Coster
Summary: Replication of repetitive DNA sequences was reconstituted in vitro using purified components. It was found that DNA alone was sufficient to induce replication stalling. Both stalling and recovery were dictated by the capacity of DNA to fold into unusual secondary structures.
NATURE COMMUNICATIONS
(2022)
Article
Chemistry, Analytical
Narges Asefifeyzabadi, Torrey E. Holland, Poopalasingam Sivakumar, Saikat Talapatra, Ishani M. Senanayake, Boyd M. Goodson, Mohtashim H. Shamsi
Summary: DNA adsorption on oxidized graphene surfaces significantly enhances electrochemical charge transfer, leading to amplified current response from redox probes. Inkjet-printed GO(x) electrodes show promise in fabricating highly sensitive platforms for label-free and chemisorption-free DNA biosensing applications.
Article
Multidisciplinary Sciences
Yogeeshwar Ajjugal, Kripi Tomar, D. Krishna Rao, Thenmalarchelvi Rathinavelan
Summary: The study investigates the influence of A...A mismatch in DNA, revealing that it may experience various transient events during B-Z junction formation, and could adapt to the DNA environment through conformational transitions. Experimental results also show that an increase in the number of A...A mismatches deepens the extent of B-Z junction, and the protein binding process commences with B-Z junction recognition.
SCIENTIFIC REPORTS
(2021)
Review
Microbiology
John P. Pribis, Yin Zhai, P. J. Hastings, Susan M. Rosenberg
Summary: This article reviews recent advances in antibiotic-induced mutagenesis, discussing the roles of ROS, SOS and general stress responses, and multichromosome cells in stress-response-induced repair of DNA breaks. It also explores other evolvable subpopulations and potential targets for drugs to slow down the evolution of antibiotic resistance.
Article
Biochemistry & Molecular Biology
Feifei Zhao, Sara Whiting, Sarah Lambourne, R. John Aitken, Ying-pu Sun
Summary: The study demonstrates that melatonin can protect human sperm from heat-induced oxidative stress by suppressing sperm mitochondrial ROS generation, increasing mitochondrial membrane potential, reducing lipid peroxidation product 4-HNE formation, and decreasing sperm DNA damage and apoptosis. This suggests melatonin as a potential treatment option for male infertility caused by heat-induced oxidative stress.
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Gabriel Matos-Rodrigues, Niek van Wietmarschen, Wei Wu, Veenu Tripathi, Natasha C. Koussa, Raphael Pavani, William J. Nathan, Elsa Callen, Frida Belinky, Ashraf Mohammed, Marek Napierala, Karen Usdin, Aseem Z. Ansari, Sergei M. Mirkin, Andre Nussenzweig
Summary: DNA can become single stranded during replication, transcription, and repair. Stable regions of single stranded DNA (ssDNA) have been identified in the human genome, including cruciform DNA and triple-stranded DNA (H-DNA). These structures contribute to genome instability.
Article
Environmental Sciences
Hayati Arda, Oguzhan Doganlar
Summary: This study explored the therapeutic potential and mechanisms of action of stress-induced miRNA cocktails from Einkorn wheat on human keratinocyte cells exposed to UV-B radiation. Results showed that the wheat miRNAs could prevent lipid peroxidation, DNA damage, regulate signaling pathways, and inhibit apoptosis in UV-stressed cells, suggesting their strong potential as a novel therapy for UV stress-related skin damage.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2022)
Article
Chemistry, Medicinal
Sarah B. Krueger, Steven C. Zimmerman
Summary: This study reports a target-guided screening method based on dynamic covalent chemistry for identifying multitarget inhibitors. A library of amine- or aldehyde-containing fragments was synthesized and assembled, resulting in a diverse set of hit combinations. These hit combinations selectively and cooperatively inhibited transcription of myotonic dystrophy type 1 and Huntington's disease repeat sequences in vitro.
JOURNAL OF MEDICINAL CHEMISTRY
(2022)
Article
Multidisciplinary Sciences
Patrik F. Viana, Eliana Feldberg, Fabio Hiroshi Takagui, Sabrina Menezes, Richard C. Vogt, Tariq Ezaz
Summary: The Matamata, also known as Chelus, is an emblematic species among Neotropical chelids. Little is known about its natural history, including its chromosomal features and sex determining strategies. This study examined the karyotype of Chelus fimbriata and Chelus orinocensis and identified a genetic sex determining mechanism with a micro XY sex chromosome system in both species. This XY system likely dates back to the earliest lineages of Chelid species.
SCIENTIFIC REPORTS
(2022)
Review
Oncology
Cristina Mitric, Lina Salman, Lusine Abrahamyan, Soyoun Rachel Kim, Petros Pechlivanoglou, Kelvin K. W. Chan, Lilian T. Gien, Sarah E. Ferguson
Summary: The study investigates the prevalence of mismatch repair protein deficiency (MMRd), microsatellite instability (MSI), and Lynch syndrome (LS) in epithelial ovarian cancer (EOC) and their diagnostic accuracy. It also examines the prevalence of MMRd, MSI-high, and LS in synchronous ovarian endometrial cancer and in histological subtypes.
GYNECOLOGIC ONCOLOGY
(2023)
Article
Genetics & Heredity
In K. Cho, Charles A. Easley, Anthony W. S. Chan
Summary: This study investigated the efficacy of aspirin and araC on stabilizing trinucleotide repeats (TNRs) in spermatogenic cells using an in vitro spermatogenesis model. The results showed that aspirin and araC can significantly stabilize TNR in Huntington's disease (HD) patient-derived cells.
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
(2022)