Review
Pharmacology & Pharmacy
Jun Liu, Lili Yang, Kexin Liu, Feng Gao
Summary: Bone tissue engineering (BTE) is a promising method for treating large bone defects that cannot be healed naturally. It involves the use of progenitor/stem cells, scaffolds, and growth factors/biochemical cues. Hydrogels, with their biocompatibility and controlled properties, are commonly used in BTE for bone regeneration. Angiogenesis plays a crucial role in the success of bone reconstruction by providing essential nutrients and factors, and hydrogels have a promising role in promoting angiogenesis during BTE.
FRONTIERS IN PHARMACOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Su Hee Kim, Donghak Kim, Misun Cha, Soo Hyun Kim, Youngmee Jung
Summary: A dome-shaped elastic PLCL scaffold with a channel and pore structure was fabricated using 3D printing technology and gel pressing method. The scaffold combined with dECM hydrogels and hADSCs promoted adipogenesis and angiogenesis. In vitro and in vivo studies showed promising results for tissue regeneration, indicating potential clinical application.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biotechnology & Applied Microbiology
Kihoon Nam, Harim T. Dos Santos, Frank Maslow, Bryan G. Trump, Pedro Lei, Stelios T. Andreadis, Olga J. Baker
Summary: Chemically conjugated Laminin-1 peptides in fibrin hydrogels promote tissue regeneration and enhance functionality of irradiated salivary glands. Untreated irradiated salivary glands experience loss in structure and functionality.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Nanoscience & Nanotechnology
Jixing Ye, Ningyuan Liu, Zongxin Li, Liehua Liu, Ming Zheng, Xueping Wen, Nan Wang, Yanqin Xu, Biemin Sun, Qiang Zhou
Summary: In this study, an injectable, hierarchically degradable bioactive scaffold was developed to simulate the natural process of autologous bone repair. The scaffold exhibited superior mechanical properties and promoted cell proliferation and osteogenic differentiation. It provided even and complete repair of bone defects and showed promising potential for bone regeneration.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Review
Biochemistry & Molecular Biology
Parisa Khayambashi, Janaki Iyer, Sangeeth Pillai, Akshaya Upadhyay, Yuli Zhang, Simon D. Tran
Summary: Tissue engineering has a long history in regenerative medicine, and methods such as cell-encapsulated hydrogels and exosomes can enhance the regenerative potential of various tissues, with hydrogels being the most user-friendly, economical, and accessible material for encapsulation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Engineering, Biomedical
Hang Xue, Zhenhe Zhang, Ze Lin, Jin Su, Adriana C. Panayi, Yuan Xiong, Liangcong Hu, Yiqiang Hu, Lang Chen, Chenchen Yan, Xudong Xie, Yusheng Shi, Wu Zhou, Bobin Mi, Guohui Liu
Summary: In this study, multifaceted nanohybrids 5QCS-1GO-PDA were synthesized and showed excellent biocompatibility and enhanced healing ability. These nanohybrids can promote angiogenesis, bone regeneration, and macrophage polarization through multiple signaling pathways, providing a promising method for effectively treating tissue defects.
BIOACTIVE MATERIALS
(2022)
Review
Biochemistry & Molecular Biology
Sourav Mohanto, Soumya Narayana, Khushboo Paresh Merai, Jahanvee Ashok Kumar, Adrija Bhunia, Umme Hani, Adel Al Fatease, B. H. Jaswanth Gowda, Sagnik Nag, Mohammed Gulzar Ahmed, Karthika Paul, Lalitkumar K. Vora
Summary: A gelatin-based hydrogel system is a biocompatible and biodegradable polymer system that responds to stimuli and has solid-like rheology. It can entangle moisture and gradually assemble a hierarchical crosslinked structure, allowing for controlled release of encapsulated payloads and providing mechanical signals to adjacent cells. The use of functional tunable biopolymers in hydrogels has increased their porosity and mechanical ability, enabling higher loading of therapeutic molecules. This review presents gelatin-based hydrogel as a potential biomaterial for various biomedical applications, highlighting its sources, structural characteristics, challenges, and designs for drug delivery.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Review
Clinical Neurology
Ryan Jarrah, Sally El Sammak, Chiduziem Onyedimma, Abdul Karim Ghaith, F. M. Moinuddin, Archis R. Bhandarkar, Ahad Siddiqui, Nicolas Madigan, Mohamad Bydon
Summary: Alginate hydrogels have been shown to be efficient and promising substrates for various applications in the treatment of spinal cord injury, including tissue engineering, drug delivery, neural regeneration, and cell-based therapies. They have the potential to improve clinical outcomes as a pro-regenerative and anti-degenerative agent.
Review
Polymer Science
Dimas Ilham Hutomo, Lisa Amir, Dewi Fatma Suniarti, Endang Winiati Bachtiar, Yuniarti Soeroso
Summary: This systematic review assessed the application of hydrogel scaffold for gingival regeneration and analyzed experimental studies on the physical and biological properties. The incorporation of natural polymers such as collagen, chitosan, and hyaluronic acids improved biomaterial characteristics, while the use of synthetic polymers faced some drawbacks. Peptides such as growth factors and arginine-glycine-aspartic acid (RGD) enhanced cell adhesion and migration. Overall, these studies successfully demonstrated the potential of hydrogel and highlighted essential biomaterial properties for future periodontal regenerative treatment.
Article
Engineering, Biomedical
Marianna Sofman, Alexander Brown, Linda G. Griffith, Paula T. Hammond
Summary: Three-dimensional micro-physiological in vitro models, such as embedding degradable hydrogel microbeads coated with induced pluripotent stem cell-derived endothelial cells, have the potential to drive angiogenesis and support vascularization of cell-dense tissues with high metabolic demand.
Article
Engineering, Biomedical
Shi Liu, Zhengzhe Han, Dapeng Zhang, Xianglong Li, Yuanyuan Cao, Jinghuan Huang, Yongsheng Li
Summary: Osteogenesis, osteoclastogenesis, and angiogenesis are crucial for bone regeneration. A novel NIR-activatable scaffold with dual-mode PTHrP-2 release capability was developed to promote osteogenesis and angiogenesis for high-efficacy bone regeneration. In vivo experiments confirmed its significant improvement of bone reparative effect.
BIOACTIVE MATERIALS
(2023)
Review
Materials Science, Biomaterials
Dinglingge Cao, Jiandong Ding
Summary: This review provides an overview of the development and applications of biomaterials, with a focus on recent advancements in medical materials. The interactions between cells and biomaterials, as well as the use of biomaterials in public health emergencies, are highlighted. The review also suggests future directions for research and development in the field.
REGENERATIVE BIOMATERIALS
(2022)
Review
Engineering, Biomedical
Xiurui Zhang, Yuwei Liu, Karen L. Clark, Anne-Marie Padget, Peter G. Alexander, Jianwu Dai, Weimin Zhu, Hang Lin
Summary: Mesenchymal stem cell-derived extracellular matrix (mECM) is gaining attention in tissue engineering due to its bioactivity, biocompatibility, and potential for autologous tissue engineering. However, current mECM methods have limitations such as low mechanical properties and lack of internal architecture. This review summarizes the development of mECM, recent methods to improve its properties, and proposes future studies for its application in regenerating tissues in humans.
BIOMEDICAL MATERIALS
(2021)
Article
Engineering, Biomedical
Ying Wang, Ranjith Kumar Kankala, Caiwen Ou, Aizheng Chen, Zhilu Yang
Summary: This review focuses on the application and advantages of hydrogels in tissue vascularization, including the creation of highly complex three-dimensional tissues and organs, tunable physical properties, and high similarity to physiological environments. Furthermore, the utilization of hydrogel-based vascularized tissues in various applications such as tissue regeneration, drug screening, and organ-on-chips is discussed.
BIOACTIVE MATERIALS
(2022)
Article
Oncology
Zuzana Demcisakova, Lenka Luptakova, Zuzana Tirpakova, Alena Kvasilova, Lubomir Medvecky, Ward De Spiegelaere, Eva Petrovova
Summary: The chorioallantoic membrane (CAM) is widely used as an in vivo model to study angiogenesis and its inhibition. This study evaluates the angiogenic potential of an acellular biopolymer scaffold using the CAM assay, showing promising results for the treatment of hard tissue defects.
Review
Chemistry, Multidisciplinary
Tamara Birman, Dror Seliktar
Summary: Balancing between chemical and biological functionality is crucial to achieve injectability in hydrogels through specific crosslinking pathways, while maintaining cell compatibility. With the emergence of new injectable biosynthetic materials, their impact in cell therapy and bioprinting is becoming increasingly significant.
ADVANCED FUNCTIONAL MATERIALS
(2021)
Article
Cell Biology
Sonia Melino, Dror Seliktar
CELL DEATH & DISEASE
(2021)
Article
Medicine, General & Internal
Liat Fellus-Alyagor, Inbal E. Biton, Hagit Dafni, Filip Bochner, Ron Rotkopf, Nava Dekel, Michal Neeman
Summary: This study characterized the early changes in blood vessel properties upon the establishment of dominance in the mouse ovary and investigated the vascular phenotype for prediction of ovulating follicles. The neo-vasculature during dominant follicle selection showed low blood volume and permeability, while the vasculature of the corpus luteum exhibited higher blood volume but similar permeability. The study showed that dominant follicles have relatively impermeable vasculature and delayed contrast enhancement in their antrum, which may aid in clinical prediction of follicular dominance and infertility treatment.
FRONTIERS IN MEDICINE
(2021)
Article
Cell & Tissue Engineering
Talia Cohen, Olga Kossover, Eli Peled, Tova Bick, Lena Hasanov, Tan Tuan Chun, Simon Cool, Dina Lewinson, Dror Seliktar
Summary: The study investigates the ability to repair critical-sized long-bone injuries using hydrogel biomaterials. It suggests that sustained delivery of rhBMP2 enhances osteogenesis and is superior to hydrogel implants containing hEPCs in promoting bone regeneration.
JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE
(2022)
Article
Obstetrics & Gynecology
Lea Nevo, Adva Cahen-Peretz, Olesya Vorontsov, Rachelli Frenkel, Maor Kabessa, Sarah M. Cohen, Adar Hamrani, Esther Oiknine-Djian, Michal Lipschuetz, Debra Goldman-Wohl, Asnat Walfisch, Michal Kovo, Michal Neeman, Simcha Yagel, Dana G. Wolf, Ofer Beharier
Summary: This study aimed to investigate the dynamics of anti-SARS-CoV-2 antibody levels following SARS-CoV-2 infection during pregnancy and the effect of a single post-infection vaccine booster dose on antibody levels in parturients. The results showed that maternal antibody levels decline during pregnancy, but a booster dose of the vaccine can significantly increase antibody levels in both mothers and newborns.
AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY
(2022)
Article
Polymer Science
Shani Attias Cohen, Haneen Simaan-Yameen, Claudia Fuoco, Cesare Gargioli, Dror Seliktar
Summary: This study presents a hydrogel-based strategy for encapsulating therapeutic AONs, which can enhance stability and cellular uptake. The injectable PF microspheres can sustainably release the AONs and achieve cellular uptake, making it a promising approach for treating DMD.
EUROPEAN POLYMER JOURNAL
(2022)
Article
Engineering, Biomedical
Raziel Hamami, Haneen Simaan-Yameen, Cesare Gargioli, Dror Seliktar
Summary: This study compares four different bioprocessing techniques for producing hydrogel microcarriers, aiming to achieve effective cell encapsulation while maintaining high cell viability and small particle size for injectability. The results show that the reverse thermal gelation technique produces cell-laden microgels with minimal specialized equipment and bioprocessing steps. The average microgel size is significantly smaller and more uniform when produced without cells.
REGENERATIVE ENGINEERING AND TRANSLATIONAL MEDICINE
(2022)
Article
Cell Biology
Shani Attias Cohen, Orit Bar-Am, Claudia Fuoco, Galit Saar, Cesare Gargioli, Dror Seliktar
Summary: This study further investigated the delivery strategy of using PF microspheres as a carrier for AON therapy in DMD. The results showed that the use of PF microspheres as a carrier improved the efficacy of AON therapy, restoring Dystrophin expression in mdx mice muscles and improving tissue histopathology. Intra-femoral injection of the microspheres demonstrated better results compared to intramuscular injection.
CELL DEATH & DISEASE
(2022)
Article
Immunology
Irit Avni Biron, Yair Maayan, Tali Mishael, Eran Hadar, Michal Neeman, Romina Plitman Mayo, Hen Y. Sela, Simcha Yagel, Rosalind Goldenberg, Ami Ben Ya'acov, Sorina Grisaru Granovsky, Jacob E. Ollech, Hadar Edelman-Klapper, Keren Masha Rabinowitz, Maor H. Pauker, Henit Yanai, Sophy Goren, Dani Cohen, Iris Dotan, Ariella Bar-Gil Shitrit
Summary: This study assessed the serologic response to two doses of the mRNA SARS-CoV-2 BNT162b2 vaccine in pregnant women with IBD compared to pregnant women without IBD and non-pregnant women with IBD. The results showed that all patients with IBD mounted a serologic response and the interval between vaccine administration and serology assessment was the most important factor determining antibody levels. Cord blood antibody levels exceeded maternal levels in both groups. It was suggested that pregnant women with IBD vaccinated at early stages of pregnancy should consider a third vaccine dose.
Article
Medicine, Research & Experimental
Adva Cahen-Peretz, Lilah Tsaitlin-Mor, Hadas Allouche Kam, Racheli Frenkel, Maor Kabessa, Sarah M. Cohen, Michal Lipschuetz, Esther Oiknine-Djian, Sapir Lianski, Debra Goldman-Wohl, Asnat Walfisch, Michal Kovo, Michal Neeman, Dana G. Wolf, Simcha Yagel, Ofer Beharier
Summary: This study aimed to investigate the effects of a third dose of the mRNA Pfizer BNT162b2 vaccine during pregnancy on maternal and neonatal immunity. The study found that the third dose significantly increased levels of antibodies in both maternal and cord blood, and this effect remained significant even when controlling for the trimester of last exposure. Mild side effects were reported following the third dose compared to the second dose.
Article
Cell Biology
Alessio Reggio, Francesca De Paolis, Salma Bousselmi, Felice Cicciarelli, Sergio Bernardini, Alberto Rainee, Dror Seliktar, Stefano Testa, Carmine Cirillo, Paolo Grumati, Stefano Cannata, Claudia Fuoco, Cesare Gargioli
Summary: In human dystrophies, ectopic deposition of fat and fibrous tissue from FAPs exacerbates progressive muscle wasting. LY2090314 interferes with adipogenesis and has the potential to limit FAP-derived fat infiltrates in dystrophic muscles.
DISEASE MODELS & MECHANISMS
(2023)
Article
Engineering, Biomedical
Revital Goldshmid, Haneen Simaan-Yameen, Liaura Ifergan, Claudia Loebel, Jason A. Burdick, Dror Seliktar
Summary: This study evaluates how mechanical properties of a scaffold can alter the phenotype and genotype of human mesenchymal stromal cells (hMSCs), aiming to enhance their differentiation potential along the myogenic, neurogenic, or chondrogenic lineages. The shear storage modulus of a hydrogel scaffold was controlled by adjusting the amount of cross-linker, and it was found that materials with lower storage moduli promoted neurogenic differentiation, while those with intermediate moduli favored myogenic differentiation, and higher moduli favored chondrogenic differentiation.
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
(2023)
Editorial Material
Biotechnology & Applied Microbiology
Ofer Beharier, Michal Neeman
Article
Medicine, Research & Experimental
Ofer Beharier, Romina Plitman Mayo, Tal Raz, Kira Nahum Sacks, Letizia Schreiber, Yael Suissa-Cohen, Rony Chen, Rachel Gomez-Tolub, Eran Hadar, Rinat Gabbay-Benziv, Yuval Jaffe Moshkovich, Tal Biron-Shental, Gil Shechter-Maor, Sivan Farladansky-Gershnabel, Hen Yitzhak Sela, Hedi Benyamini-Raischer, Nitzan D. Sela, Debra Goldman-Wohl, Ziv Shulman, Ariel Many, Haim Barr, Simcha Yagel, Michal Neeman, Michal Kovo
Summary: The study demonstrates that the BNT162b2 mRNA vaccine induces a strong maternal IgG response that crosses the placenta barrier to reach fetal titers within 15 days of the first dose. The transfer ratio of anti-COVID-19 antibodies from mother to neonate differs between vaccination and infection groups, with lower IgG transfer ratio for third-trimester compared to second-trimester infection. Additionally, fetal IgM response was only detected in neonates from the infected group.
JOURNAL OF CLINICAL INVESTIGATION
(2021)
Meeting Abstract
Developmental Biology
Lital Ben Moyal, Talia Harris, Hagit Dafni, Michal Neeman