Journal
JOURNAL OF TRANSLATIONAL MEDICINE
Volume 16, Issue -, Pages -Publisher
BMC
DOI: 10.1186/s12967-018-1600-x
Keywords
Myalgic encephalomyelitis/chronic fatigue syndrome; Long non-coding RNA; Expression signature; Peripheral blood mononuclear cells
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Funding
- Ministry of Science and Technology (MOST), Taiwan [106-2314-B-039-047-MY3]
- Lost Voices Foundation e.V., Germany
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Background: Myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS) is a chronic debilitating disease with huge social-economic impact. It has been suggested that immune dysregulation, nitrooxidative stress, and metabolic impairment might contribute to disease pathogenesis. However, the etiology of ME/CFS remains largely unclear, and diagnostic/prognostic disease markers are lacking. Several long noncoding RNAs (lncRNA, > 200 bp) have been reported to play roles in immunological diseases or in stress responses. Methods: In our study, we examined the expression signature of 10 very long lncRNAs (>5 kb, CR933609, His-RNA, AK124742, GNAS1-AS, EmX20S, MIAT,TUG1, NEAT1, MALAT1, NTT) in the peripheral blood mononuclear cells of 44 ME/CFS patients. Results: LncRNAs NTT, MIAT and EmX2OS levels were found to be significantly elevated in ME/CFS patients as compared with healthy controls. Furthermore, NTT and EmX2OS levels increased with disease severity. Stimulation of human monocytic cell line THP-1 and glioma cell line KALS1 with H2O2 (oxidative stress) and poly (I:C) (double strand RNA, representing viral activation) increased the expression levels of NTT and MIAT. Conclusions: Our study revealed a ME/CFS-associated very long lncRNA expression signature, which might reflect the regulatory response in ME/CFS patients to oxidative stress, chronic viral infection and hypoxemia. Further investigations need to be done to uncover the functions and potential diagnostic value of these lncRNAs in ME/CFS.
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