Article
Biochemistry & Molecular Biology
Pia Wilhelmi, Varun Giri, Franziska Maria Zickgraf, Volker Haake, Stefan Henkes, Peter Driemert, Paul Michaelis, Wibke Busch, Stefan Scholz, Burkhard Flick, Marta Barenys, Barbara Birk, Hennicke Kamp, Robert Landsiedel, Dorothee Funk-Weyer
Summary: Through LC-MS/MS and GC-MS metabolomics, this study investigated the changes in endogenous metabolites in zebrafish embryos as indicators of developmental toxicity pathways. The results showed that exposure to the compound PTU led to morphological alterations and significant metabolic changes, which could be linked to the inhibition of thyroid peroxidase and potential neurodevelopmental impairments. This study demonstrated that metabolite changes in zebrafish embryos provide mechanistic information associated with the mode of action of PTU.
CHEMICO-BIOLOGICAL INTERACTIONS
(2023)
Review
Environmental Sciences
Juanmei Gao, Wanhua Shen
Summary: The Xenopus model is advantageous for studying embryo development and developmental toxicity, as well as human diseases. It has been successfully used to study various diseases, including fetal alcohol spectrum disorders, autism, epilepsy, and cardiovascular disease. Xenopus also has potential applications in genetic and chemical screening.
ENVIRONMENTAL POLLUTION
(2021)
Article
Environmental Sciences
Chuanxin Zhang, Xueqi Dong, Xiaoyu Yin, Xinyi Yuan, Jiawei Wang, Jinzhu Song, Zhenzhen Hou, Cheng Li, Keliang Wu
Summary: 2-bromoacetamide (BAcAm), a new class of disinfection by-products (DBPs), is commonly found in drinking water worldwide. The high cytogenetic toxicity of BAcAm has raised concerns about its toxic effects on early embryonic development. In this study, we developed an optimized in vitro culture (IVC) system for peri-and early post-implantation mouse embryos and used it to assess the developmental toxicity of BAcAm. We observed that exposure to BAcAm led to a decrease in egg cylinder formation rate and aberrant lineage differentiation in a dose-dependent manner. Transcriptomic analysis further revealed that BAcAm exposure during early developmental stages affected the abundance of transcripts related to various biological processes, indicating its toxic effects on embryonic development. Therefore, this study provides a robust tool for studying the toxicology of chemicals during early embryonic development and highlights the developmental toxicity of BAcAm in mammals.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2023)
Article
Agriculture, Dairy & Animal Science
Yao Xiao, Froylan Sosa, Lesley R. de Armas, Li Pan, Peter J. Hansen
Summary: Gene expression analysis in preimplantation embryos using a direct synthesis and specific-target pre-amplification method showed reliable results for sexing and other gene expression studies. Calibration curve analysis of PCR results validated 93.75% of genes tested, demonstrating the method's robustness. The study also showed that within-assay variation increased when cycle threshold values exceeded 18, indicating limitations in sensitivity at higher levels of gene expression.
JOURNAL OF DAIRY SCIENCE
(2021)
Article
Environmental Sciences
Kaori Chigusa, Kazuki Kanda, Hisato Iwata
Summary: This study investigated the toxicity of TCIPP on early chicken embryos using a shell-less incubation system. The results showed that TCIPP exposure led to decreased survival rate, reduced body and organ length, impaired blood vessel and red blood cell development, decreased heart rate, asymmetrical somite formation, and impaired eye development in avian embryos.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2023)
Review
Developmental Biology
Mirjam Niethammer, Tanja Burgdorf, Elisa Wistorf, Gilbert Schoenfelder, Mandy Kleinsorge
Summary: This article discusses the application of in vitro models in developmental toxicity testing, questioning the relevance of adverse reactions in animals to humans. It also points out that the currently developed cell culture-based models of embryo-fetal development lack sufficient levels of standardization and reproducibility.
Article
Biochemistry & Molecular Biology
Kaikai Liu, Jingjing Song, Wendan Chi, Hongjun Liu, Shanshan Ge, Daode Yu
Summary: The study found that nickel exposure had significant toxic effects on the early development of marine fish, affecting egg size, heart rate, hatchability, deformity rate, and total body length of newly hatched larvae. Embryos were more sensitive to nickel than larvae, with specific genes showing altered expressions in response to nickel exposure.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
(2021)
Article
Environmental Sciences
Fei Liu, Ying Zhang, Fan Wang
Summary: This study exposed zebrafish embryos to TCS and evaluated its developmental toxicity. The results showed that TCS can induce oxidative stress and apoptosis, potentially through the ROS-p53-caspase dependent pathway.
ENVIRONMENTAL TOXICOLOGY
(2022)
Article
Environmental Sciences
Xian Qin, Huiju Lin, Yaru Cao, Rudolf Shiu Sun Wu, Keng Po Lai, Richard Yuen Chong Kong
Summary: Exposure to 17 alpha-ethinylestradiol (EE2) in fish can result in developmental toxicity, including growth retardation, decreased heart rate, delayed eye pigmentation, reduced hatching rate, and impaired larval locomotion. Transcriptional analysis revealed that these impairments are controlled by estrogen receptor and related to eye structure, neuronal and synaptic structure, and behavior. Key factors identified, such as PRKAA2, APOB, EPHB2, OXTR, NR2E3, and POU4F2, could serve as biomarkers for assessing EE2-induced embryo developmental toxicity.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Genetics & Heredity
Jada Lindsay Nix, Gustavo Pimenta Schettini, Fernando Henrique Biase
Summary: This article introduces the methods of using RNA-sequencing data or PCR assays to determine the presence of the chromosome Y in samples and emphasizes the importance of these methods for studying biological variation due to sexual dimorphism. The authors successfully identified a set of genes exclusively expressed in male tissues using the sequence of the cattle ChrY and transcriptome data, and used them to determine the sex of 22 cattle blastocysts. Additionally, they accurately identified the sex of cattle blastocysts using a pair of oligonucleotides in a PCR assay. These methods have important applications for researchers using RNA-sequencing data or DNA to determine the sex of samples. Evaluation: 9 points.
FRONTIERS IN GENETICS
(2023)
Article
Multidisciplinary Sciences
Monika Stachowiak, Izabela Szczerbal, Joanna Nowacka-Woszuk, Tomasz Nowak, Natalia Sowinska, Anna Lukomska, Maciej Gogulski, Malgorzata Badura, Karolina Sklorz-Mencel, Dariusz Jagodka, Wojciech Nizanski, Stanislaw Dzimira, Marek Switonski
Summary: The genetic background of feline disorders of sex development (DSDs) was investigated, revealing that abnormalities of sex chromosomes are important causes of feline DSDs. Additionally, a specific variant of the LHCGR gene was identified as a potential marker associated with the XY DSD phenotype.
SCIENTIFIC REPORTS
(2022)
Article
Agriculture, Dairy & Animal Science
Irene Estelles-Domingo, Pascual Lopez-Lopez
Summary: This study used biometric analysis to accurately determine the sex of Bonelli's eagles using just two measurements. The study included 137 eagles sampled in eastern Spain from 2015 to 2022. The results showed that the lateral tarsus length and dorso-ventral tarsus length were the most effective measurements for sex determination. This non-invasive method has multiple applications in conservation efforts.
Article
Veterinary Sciences
Wataru Hirata, Taiki Tomoda, Shunsuke Yuri, Ayako Isotani
Summary: By introducing a red fluorescent protein (RFP) transgene on the Y-chromosome, a mouse model was successfully established to rapidly and accurately identify male embryos. However, the Y-RFP mouse exhibited a lethal phenotype after birth, but no lethal phenotypes were observed when including the mitochondrial locating signal tdTomato (mtRFP) in the transgene construct. When crossed with wild-type females, about half of the fertilized eggs from Y-mtRFP male mice showed an RFP signal at the preimplantation stage, allowing for the recognition of XY eggs as RFP-positive embryos. Additionally, 100% sexing was achieved at the preimplantation stage using X-linked GFP/Y-linked RFP male mice. The established Y-mtRFP mouse models can be utilized for research related to sex chromosomes.
EXPERIMENTAL ANIMALS
(2022)
Article
Biochemistry & Molecular Biology
Mingxing Wang, Juan Liu, Huiyun Wang, Tingzhang Hu
Summary: This study investigated the effects of Spiromesifen (SPF) on blood vessel development and its mechanism of action. It was found that SPF exposure caused abnormal blood vessel development, inhibited cell migration and proliferation, and disrupted the cytoskeleton via the Rho/ROCK pathway. These findings contribute to a better understanding of the toxic effects of SPF on angiocardiopathy.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2022)
Article
Environmental Sciences
Lizhen Zhu, Chengju Wang, Hongyun Jiang, Lan Zhang, Liangang Mao, Yanning Zhang, Suzhen Qi, Xingang Liu
Summary: Quizalofop-P-ethyl (QpE), a highly efficient selective herbicide, was found to have toxic effects on zebrafish embryos, including increased mortality rate, decreased hatching rate, morphological defects, and severe disruption in cardiovascular development. QpE exposure also led to inflammatory responses in the heart and altered the expression of genes related to heart development and inflammation, potentially affecting embryo development. These findings are important for assessing the effects of QpE on early development of aquatic organisms and designing risk management measures.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2022)
Article
Chemistry, Organic
Safwan Aroua, Andrew N. Lowell, Ankita Ray, Nils Trapp, W. Bernd Schweizer, Marc-Olivier Ebert, Yoko Yamakoshi
Article
Biology
Chiemi Toyama-Mori, Kurumi Suzuki, Yoko Miyazaki, Takehito Suzuki, Masaya Katsumata, Kazuaki Tanaka, Makoto Usami, Tatsuya Takizawa
Article
Chemistry, Multidisciplinary
Safwan Aroua, Ankita Ray, Marc-Olivier Ebert, Yoko Yamakoshi
HELVETICA CHIMICA ACTA
(2019)
Article
Chemistry, Multidisciplinary
Olesya Semivrazhskaya, Adrian Romero-Rivera, Safwan Aroua, Sergey I. Troyanov, Marc Garcia-Borras, Steven Stevenson, Silvia Osuna, Yoko Yamakoshi
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2019)
Article
Chemistry, Organic
Korinne Liosi, Adrian Romero-Rivera, Olesya Semivrazhskaya, Caravaggio D. Caniglia, Marc Garcia-Borras, Nils Trapp, Silvia Osuna, Yoko Yamakoshi
Article
Biochemistry & Molecular Biology
Takumi Okubo, Shinri Fujimoto, Daiki Hayashi, Takehito Suzuki, Motoharu Sakaue, Yoko Miyazaki, Kazuaki Tanaka, Makoto Usami, Tatsuya Takizawa
NITRIC OXIDE-BIOLOGY AND CHEMISTRY
(2019)
Article
Chemistry, Multidisciplinary
Olesya Semivrazhskaya, Safwan Aroua, Maxim Yulikov, Adrian Romero-Rivera, Steven Stevenson, Marc Garcia-Borras, Silvia Osuna, Yoko Yamakoshi
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2020)
Article
Chemistry, Physical
Ankita Ray, Korinne Liosi, Shivaprakash N. Ramakrishna, Nicholas D. Spencer, Akinori Kuzuya, Yoko Yamakoshi
JOURNAL OF PHYSICAL CHEMISTRY LETTERS
(2020)
Article
Biochemistry & Molecular Biology
Daiki Hayashi, Takumi Okubo, Takehito Suzuki, Yoko Miyazaki, Kazuaki Tanaka, Makoto Usami, Tatsuya Takizawa
Summary: Valproic acid promotes neuronal differentiation of ASCs through up-regulated iNOS and the NO-citrulline cycle. Mechanisms involve increased expression of ASS and iNOS by VPA, leading to continuous NO production and potent signaling for neuronal differentiation. This suggests a mechanism allowing short-lived NO to function conveniently as a potent signaling molecule that can disappear quickly after its role.
NITRIC OXIDE-BIOLOGY AND CHEMISTRY
(2021)
Article
Nanoscience & Nanotechnology
Alessandro Fracassi, Ankita Ray, Nako Nakatsuka, Cristiana Passiu, Matthias Tanriver, Dominik Schauenburg, Simon Scherrer, Anissa Ouald Chaib, Joydeb Mandal, Shivaprakash N. Ramakrishna, Jeffrey W. Bode, Nicholas D. Spencer, Antonella Rossi, Yoko Yamakoshi
Summary: The efficient and bioorthogonal chemical ligation reaction between potassium acyltrifluoroborates (KATs) and hydroxylamines (HAs) was used for the surface functionalization of a self-assembled monolayer (SAM) with biomolecules. The results demonstrate the versatility of the KAT ligation for the covalent attachment of a variety of water-soluble molecules onto SAM surfaces under dilute and biocompatible conditions to form stable, natural amide bonds. Despite the presence of unprotected functional groups within their structures, the surface KAT ligation proceeded rapidly in a chemoselective manner.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Chemistry, Multidisciplinary
Ankita Ray, Cristiana Passiu, Masayuki Nasuda, Shivaprakash N. Ramakrishna, Antonella Rossi, Akinori Kuzuya, Nicholas D. Spencer, Yoko Yamakoshi
Summary: A novel tripod molecule incorporating a C-60 photocatalyst was designed and immobilized onto an AFM tip for single-molecule level oxidative damage of DNA origami. The localized and temporally controlled oxidation of DNA origami was successfully achieved through singlet-oxygen generation from immobilized C-60 on the AFM tip under visible-light irradiation. This method allows for manipulation of various species on surfaces via controlled oxidation reactions triggered by locally generated singlet-oxygen from C-60 on the AFM tip.
Article
Chemistry, Multidisciplinary
Korinne Liosi, Anton J. Stasyuk, Fabio Masero, Alexander A. Voityuk, Thomas Nauser, Victor Mougel, Miquel Sola, Yoko Yamakoshi
Summary: The study found that photoinduced cleavage of DNA by C-60-PEG was higher than that by C-70-PEG under visible light irradiation, especially in the presence of an electron donor. The preference of photoinduced ROS generation from fullerene-PEG conjugates was dependent on the fullerene core and functionalization pattern of C-70, with different pathways (type I versus type II) determined by electron transfer rates and computationally estimated reaction rates.
Article
Toxicology
Atsuko Miyajima, Yukie Kuroda, Kazue Sakemi-Hoshikawa, Makoto Usami, Katsuyoshi Mitsunaga, Tomohiko Irie, Yasuo Ohno, Momoko Sunouchi
TOXICOLOGY REPORTS
(2020)
Article
Chemistry, Multidisciplinary
Alessandro Fracassi, Jianbo Cao, Naoko Yoshizawa-Sugata, Eva Toth, Corey Archer, Olivier Groninger, Emanuela Ricciotti, Soon Yew Tang, Stephan Handschin, Jean-Pascal Bourgeois, Ankita Ray, Korinne Liosi, Sean Oriana, Wendelin Stark, Hisao Masai, Rong Zhou, Yoko Yamakoshi
Meeting Abstract
Toxicology
A. Miyajima-Tabata, K. Sakemi-Hoshikawa, M. Usami, K. Mitsunaga, T. Irie, Y. Ohno, M. Sunouchi
TOXICOLOGY LETTERS
(2019)
