Review
Oncology
Saevar Ingthorsson, Gunnhildur Asta Traustadottir, Thorarinn Gudjonsson
Summary: This review discusses the structure, function, and dynamics of the breast gland, as well as how changes in breast cell function can lead to different types of cancer. The development of the breast gland is closely related to the menstrual cycle, and stem cells and progenitor cells play important roles in the proliferation, differentiation, and involution cycles of the breast. Breast cancer originates from stem cells and progenitor cells, and both stem cells and cancer cells exhibit cellular plasticity. Additionally, the stromal environment plays a vital role in cancer initiation and progression.
Article
Biotechnology & Applied Microbiology
Filipa C. Teixeira, Sara Chaves, Ana Luisa Torres, Cristina C. Barrias, Silvia J. Bidarra
Summary: The stromal microenvironment of breast tumors plays a crucial role in tumor development and metastasis, with tumor angiogenesis requiring cooperation between various cell types and extracellular matrix. The developed hybrid alginate-based 3D system allows for the simulation and support of epithelial morphogenesis and endothelial tubulogenesis in breast tumors.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Raul Munoz Velasco, Ana Garcia Garcia, Paula Jimenez Sanchez, Inmaculada Montanuy Sellart, Victor Javier Sanchez-Arevalo Lobo
Summary: Pancreatic ductal adenocarcinoma is a challenging disease with low survival rate due to its chemoresistance and tumor microenvironment. New therapies targeting the tumor microenvironment should be developed for effective treatment.
JOURNAL OF PHYSIOLOGY AND BIOCHEMISTRY
(2023)
Article
Nanoscience & Nanotechnology
Barbara Blanco-Fernandez, Sergi Rey-Vinolas, Gulsun Bagci, Gerard Rubi-Sans, Jorge Otero, Daniel Navajas, Soledad Perez-Amodio, Elisabeth Engel
Summary: Breast tissue-derived matrices can serve as important biomaterials to replicate the complexity of the tumor extracellular matrix (ECM), with the addition of collagen type I improving the fidelity to breast tumors. These novel bioinks have great potential in bioprinting breast cancer models.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Biochemistry & Molecular Biology
Nikolaos Louros, Joost Schymkowitz, Frederic Rousseau
Summary: The number of disease-associated amyloid structures at atomic resolution has increased significantly in recent years. These structures have confirmed the polymorphic nature of amyloids and their association with specific proteinopathies. The exact nature and relationship of this association with the selective vulnerability of amyloid nucleation, propagation, and toxicity are still unknown. However, recent patient-derived amyloid structures have provided mechanistic insights, discussing the framework organization of amyloid polymorphism and how heterotypic amyloid interactions with the physiological environment could modify the solubility and assembly of amyloidogenic proteins.
CURRENT OPINION IN STRUCTURAL BIOLOGY
(2022)
Review
Oncology
Jia Ning Nicolette Yau, Giulia Adriani
Summary: Colorectal cancer (CRC) remains a major cause of death globally. Recent developments in 3D cell culture have allowed for better modeling of the tumor microenvironment, leading to more accurate predictions of tumor progression and drug response. Incorporating different cell types such as cancer-associated fibroblasts, endothelial cells, immune cells, and gut bacteria in 3D CRC spheroid models has improved their relevance and potential for identifying new therapeutic targets. This mini review discusses the advances in mimicking tumor heterogeneity in these models and highlights the impact of diverse cell populations on chemoresistance and tumor progression.
FRONTIERS IN ONCOLOGY
(2023)
Article
Materials Science, Multidisciplinary
Yutong Chen, Yuzhe Liu, Jiaxin Zhang, He Liu, Jincheng Wang, Qiran Liu, Yan Zhang
Summary: Breast reconstruction is crucial for mental health, current methods have limitations, and 3D bioprinting technology shows promise as an alternative solution.
MATERIALS & DESIGN
(2021)
Article
Biochemistry & Molecular Biology
Zuzanna Bober, Rafal Podgorski, David Aebisher, Grzegorz Cieslar, Aleksandra Kawczyk-Krupka, Dorota Bartusik-Aebisher
Summary: Noninvasive measurements of H-1 Magnetic Resonance Imaging (MRI) relaxation times were used to evaluate Trastuzumab delivery in a 3D cell culture construct. The study utilized a bioreactor for the cell cultures and compared normal and breast cancer cells. Results showed that the relaxation time of cancer cells was lower than normal cells before and after treatment. The use of H-1 MRI relaxation times allows for the visualization of cell viability in response to treatment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Engineering, Biomedical
Antonio Varone, Justin Ke Nguyen, Lian Leng, Riccardo Barrile, Josiah Sliz, Carolina Lucchesi, Norman Wen, Achille Gravanis, Geraldine A. Hamilton, Katia Karalis, Christopher D. Hinojosa
Summary: This study presents a new 3D cell-culture system that combines Organ-Chip technology with advantages of three-dimensional organotypic culture. Using microfabrication techniques, a flexible chip was engineered to recreate critical elements of native tissue architecture, offering researchers the possibility to model organs and their functionality more accurately. The Open-Top Chip design successfully addresses common challenges associated with Organs-on-Chip technology, demonstrating its feasibility with primary human skin and alveolar epithelial cells.
Article
Biotechnology & Applied Microbiology
Jae A. Mckee, Elisabet A. Olsen, Gideon Wills Kpeli, Moriah R. Brooks, Mohamadreza Beitollahpoor, Noshir S. Pesika, Matthew E. Burow, Mark J. Mondrinos
Summary: Physical characteristics of solid tumors, such as dense internal microarchitectures and pathological stiffness, play a role in cancer progression and treatment. This study demonstrates a method to engineer tissue constructs that mimic dense solid tumors by allowing contractile mesenchymal cells to deform and compact collagen-containing hydrogels co-seeded with carcinoma cell spheroids. These dense carcinoma constructs capture relevant physical characteristics of solid carcinomas and can be used for extended culture. This approach has the potential to provide clinically relevant venues for studying cancer pathophysiology and addressing challenges in drug delivery and cellular immunotherapy to solid tumors.
BIOTECHNOLOGY AND BIOENGINEERING
(2023)
Article
Engineering, Civil
Xingyan Fan, Lianghao Zou, Jie Song, Shuguo Liang, Min Yu
Summary: The present study proposes a novel three-dimensional forced vibration wind tunnel test system that can drive the model to vibrate in three dimensions while maintaining more realistic mode shapes. The test results show that the system performs well, providing stable vibration amplitude, frequency, and phase angle in all three directions. The proposed system provides an alternative method for determining the aeroelastic properties of high-rise buildings.
JOURNAL OF WIND ENGINEERING AND INDUSTRIAL AERODYNAMICS
(2023)
Article
Materials Science, Multidisciplinary
Irene Chiesa, Carmelo De Maria, Giovanni Vozzi, Riccardo Gottardi
Summary: Each year, thousands of patients face life-threatening ear, nose, and throat disorders, such as tracheal stenosis, or conditions that affect their psychosocial well-being, such as microtia. Tissue engineering offers an exciting alternative by creating bioartificial constructs using three-dimensional scaffolds and human cells that can grow and develop. Bioprinting technologies, which utilize additive manufacturing, are particularly useful in addressing the complex geometries of ear, nose, and throat, allowing for the creation of patient-specific scaffolds with high design flexibility and repeatability.
Article
Oncology
Milena Hornburg, Melanie Desbois, Shan Lu, Yinghui Guan, Amy A. Lo, Susan Kaufman, Ashley Elrod, Alina Lotstein, Teresa M. DesRochers, Jose L. Munoz-Rodriguez, Xingwei Wang, Jennifer Giltnane, Oleg Mayba, Shannon J. Turley, Richard Bourgon, Anneleen Daemen, Yulei Wang
Summary: This study provides a detailed analysis of distinct T cell infiltration patterns in ovarian tumors, revealing the impact on responses to cancer immunotherapies and the underlying key determinants and biology of tumor immune phenotypes. The research highlights potential molecular mechanisms shaping these phenotypes and may inform therapeutic strategies for enhancing clinical benefit from cancer immunotherapies.
Article
Nanoscience & Nanotechnology
Yue Gu, Chunfeng Wang, Namheon Kim, Jingxin Zhang, Tsui Min Wang, Jennifer Stowe, Rohollah Nasiri, Jinfeng Li, Daibo Zhang, Albert Yang, Leo Huan-Hsuan Hsu, Xiaochuan Dai, Jing Mu, Zheyuan Liu, Muyang Lin, Weixin Li, Chonghe Wang, Hua Gong, Yimu Chen, Yusheng Lei, Hongjie Hu, Yang Li, Lin Zhang, Zhenlong Huang, Xingcai Zhang, Samad Ahadian, Pooja Banik, Liangfang Zhang, Xiaocheng Jiang, Peter J. Burke, Ali Khademhosseini, Andrew D. McCulloch, Sheng Xu
Summary: The research introduces a scalable platform featuring a three-dimensional field-effect transistor array produced via compressive buckling, enabling accurate recording of transmembrane potentials and intracellular recordings in cells and cellular networks. The platform utilizes high-performance field-effect transistors for cellular interfacing, providing faithful recordings and valuable experimental data for probing the electrical behaviors of single cells and cellular networks. Leveraging the high spatial and temporal resolutions of the transistors, the study reveals new insights into signal conduction velocities and pathways within cells and cellular constructs.
NATURE NANOTECHNOLOGY
(2022)
Review
Biotechnology & Applied Microbiology
Hikaru Suenaga, Noritaka Kagaya, Manabu Kawada, Daisuke Tatsuda, Toshiro Sato, Kazuo Shin-ya
Summary: Recent progress in three-dimensional cell culture systems, including 3D co-culture, spheroid culture, and organoid culture, has attracted attention in basic life science and drug development. These methods enable more human tissue-like culture and have largely replaced traditional two-dimensional monolayer culture. By combining 3D culture methods with high-content imaging analysis, diverse and convincing data can be obtained even during initial screening.
JOURNAL OF ANTIBIOTICS
(2021)
Article
Biotechnology & Applied Microbiology
K. Kwist, W. C. Bridges, K. J. L. Burg
Article
Cell Biology
Richard T. Pace, Karen J. L. Burg
JOURNAL OF HISTOTECHNOLOGY
(2015)
Editorial Material
Cell Biology
Karen J. L. Burg
JOURNAL OF HISTOTECHNOLOGY
(2015)
Article
Engineering, Biomedical
Jordon Gilmore, Timothy Burg, Richard E. Groff, Karen J. L. Burg
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS
(2017)
Editorial Material
Cell Biology
Karen J. L. Burg
JOURNAL OF HISTOTECHNOLOGY
(2016)
Editorial Material
Cell Biology
Karen J. L. Burg
JOURNAL OF HISTOTECHNOLOGY
(2016)
Editorial Material
Cell Biology
Karen J. L. Burg
JOURNAL OF HISTOTECHNOLOGY
(2017)
Article
Engineering, Biomedical
Jordon Gilmore, Freda Yin, Karen J. L. Burg
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS
(2019)
Article
Cell & Tissue Engineering
Amanda Lance, Chih-Chao Yang, Muthulekha Swamydas, Delphine Dean, Sandy Deitch, Karen J. L. Burg, Didier Dreau
JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE
(2016)
Article
Multidisciplinary Sciences
Kevin J. Wu, Timothy C. Burg, Karen J. L. Burg
TECHNOLOGY AND INNOVATION
(2020)
Article
Multidisciplinary Sciences
Sarah C. Rowlinson, Timothy C. Burg, William C. Bridges, Karen J. L. Burg
TECHNOLOGY AND INNOVATION
(2019)
Article
Multidisciplinary Sciences
Neela White, Karen Burg
TECHNOLOGY AND INNOVATION
(2019)
Article
Engineering, Biomedical
Suzanne M. Tabbaa, Julia L. Sharp, Karen J. L. Burg
ANNALS OF BIOMEDICAL ENGINEERING
(2017)
Article
Engineering, Biomedical
Jonathan L. Shaul, Betsy K. Davis, Karen J. L. Burg
REGENERATIVE ENGINEERING AND TRANSLATIONAL MEDICINE
(2016)
Article
Oncology
Terrika A. Ngobili, Hiral Shah, Jang Pyo Park, Kerri W. Kwist, Beau Inskeep, Karen J. L. Burg, Brian W. Booth
ANTICANCER RESEARCH
(2015)