Article
Multidisciplinary Sciences
Konstantinos Nakos, Md Noor A. Alam, Megan R. Radler, Ilona A. Kesisova, Changsong Yang, Joshua Okletey, Meagan R. Tomasso, Shae B. Padrick, Tatyana M. Svitkina, Elias T. Spiliotis
Summary: Cellular morphogenesis and processes such as cell division and migration require the coordination of the microtubule and actin cytoskeletons. This study shows that septins mediate microtubule-actin crosstalk by coupling actin polymerization to microtubule lattices.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Review
Cell Biology
Anne-Betty Ndiaye, Gijsje H. Koenderink, Michal Shemesh
Summary: The mammalian cytoskeleton plays an important role in transmitting external forces to the cell interior and generating intracellular forces. The three interpenetrating structural proteins, actin, microtubules, and intermediate filaments, have interdependent functions and the intermediate filaments play a central role in mechanosensitivity. Cytoskeletal crosstalk regulates the stability and organization of all three filament families at different scales, and also affects cell adaptation to external cues.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Review
Cell Biology
Ji Wu, Yun-Yi Wang, Xi-Wen Yang, Xiao-Tian Zhang, Jia-Yi Tang
Summary: hNP22 is a novel neuron-specific protein that interacts with actin filaments and microtubules, showing similarities to other cytoskeleton-associated proteins. It has potential biological functions in neurological disorders like schizophrenia and ARBD, making it a target for research in exploring clinical significance.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Urko Del Castillo, Rosalind Norkett, Wen Lu, Anna Serpinskaya, Vladimir Gelfand
Summary: Ataxin-2 plays a crucial role in regulating neuronal cytoskeletal dynamics and organelle trafficking, as evidenced by fruit fly models, where its loss led to defects in nervous system development and survival.
Article
Chemistry, Physical
Gloria Lee, Gregor Leech, Pancy Lwin, Jonathan Michel, Christopher Currie, Michael J. Rust, Jennifer L. Ross, Ryan J. McGorty, Moumita Das, Rae M. Robertson-Anderson
Summary: This study combines microscale experiments with mechanistic modeling to investigate how connectivity, rigidity, and force-generation affect emergent material properties in composite networks of actin, tubulin, and myosin in the cytoskeleton. The research reveals that percolated actomyosin networks are essential for contraction, and a critical fraction of microtubules is necessary to sustain controlled dynamics. The comprehensive phase map presented not only provides important insight into different routes the cytoskeleton can use to alter its dynamics and structure, but also serves as a blueprint for designing cytoskeleton-inspired materials with tunability, resilience, and adaptability for diverse applications.
Article
Cell Biology
Maria Selvadurai, Mitchell J. Moon, Simon J. Mountford, Xiao Ma, Zhaohua Zheng, Ian G. Jennings, Natasha M. Setiabakti, Rizani P. Iman, Rose J. Brazilek, Nurul Aisha Z. Abidin, Gaetan Chicanne, Sonia Severin, Alyce J. Nicholls, Connie H. Y. Wong, Jean-Yves Rinckel, Anita Eckly, Christian Gachet, Warwick S. Nesbitt, Philip E. Thompson, Justin R. Hamilton
SCIENCE TRANSLATIONAL MEDICINE
(2020)
Review
Biochemistry & Molecular Biology
Agnes Ribes, Antoine Oprescu, Julien Viaud, Karim Hnia, Gaetan Chicanne, Jean-Marie Xuereb, Sonia Severin, Marie-Pierre Gratacap, Bernard Payrastre
BIOCHEMICAL JOURNAL
(2020)
Article
Hematology
Marion Egot, Dominique Lasne, Sonia Poirault-Chassac, Tristan Mirault, Dominique Pidard, Elise Dreano, Caroline Elie, Sophie Gandrille, Aurore Marchelli, Dominique Baruch, John Rendu, Julien Faure, Claire Flaujac, Marie-Pierre Gratacap, Pierre Sie, Pascale Gaussem, Remi Salomon, Genevieve Baujat, Christilla Bachelot-Loza
Summary: This study reveals that a deficiency in the OCRL protein results in abnormal actomyosin cytoskeleton reorganization in both platelets and megakaryocytes in patients with Lowe syndrome, affecting both thrombopoiesis and platelet responses to activation essential for hemostasis.
BRITISH JOURNAL OF HAEMATOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Silvia Arcucci, Fernanda Ramos-Delgado, Coralie Cayron, Nicole Therville, Marie-Pierre Gratacap, Celine Basset, Benoit Thibault, Julie Guillermet-Guibert
Summary: PI3Ks are important lipid kinases that produce phosphoinositides, with three classes including PI3Kα and PI3Kβ in vertebrates. These isoforms share similar protein domains and lipid substrate specificity. In addition to redundancy, PI3Kα and PI3Kβ cooperate in integrating specific signals for organ development, physiology, and disease.
BIOCHEMICAL JOURNAL
(2021)
Article
Biochemistry & Molecular Biology
Typhaine Anquetil, Romain Solinhac, Aude Jaffre, Gaetan Chicanne, Julien Viaud, Jean Darcourt, Cyrille Orset, Eva Geuss, Christoph Kleinschnitz, Bart Vanhaesebroeck, Denis Vivien, Karim Hnia, Vincent Larrue, Bernard Payrastre, Marie-Pierre Gratacap
Summary: Protecting endothelial cells is critical in preventing the impact of vascular leakage and edema on pathological conditions. PI3KC2 beta could be a potential new therapeutic target for treating aggravating lesions following ischemic stroke.
Article
Cell Biology
Fanny Vardon-Bounes, Cedric Garcia, Alexandra Piton, Jennifer Series, Marie-Pierre Gratacap, Michael Poette, Thierry Seguin, Laure Crognier, Stephanie Ruiz, Stein Silva, Jean-Marie Conil, Vincent Minville, Bernard Payrastre
Summary: This study assessed platelet activation parameters at admission and 48 hours later in patients with sepsis, revealing significantly increased platelet activation levels in patients compared to controls at both time points, but with less responsiveness to different agonists.
Review
Cell Biology
Cedric Garcia, Baptiste Compagnon, Michael Poette, Marie-Pierre Gratacap, Francois-Xavier Lapebie, Sophie Voisin, Vincent Minville, Bernard Payrastre, Fanny Vardon-Bounes, Agnes Ribes
Summary: Platelets play a crucial role in hemostasis and thrombosis, but they are also involved in mechanisms associated with inflammation, thrombosis, and the immune system in various diseases. During sepsis, platelets enhance the recruitment and activation of immune cells and contribute to pathogen elimination. However, these mechanisms can also lead to severe organ dysfunction.
Article
Hematology
Manuella Caux, Rana Mansour, Jean-Marie Xuereb, Gaetan Chicanne, Julien Viaud, Alicia Vauclard, Frederic Boal, Bernard Payrastre, Helene Tronchere, Sonia Severin
Summary: This study demonstrates that PIKfyve and its lipid products PtdIns5P and PtdIns(3,5)P-2 play crucial roles in regulating granule integrity in both megakaryocytes and platelets. Inhibition of PIKfyve leads to impaired granule integrity, affecting platelet aggregation and thrombus formation.
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Wen-Ting Lo, Hassane Belabed, Murat Kuecuekdisli, Juliane Metag, Yvette Roske, Polina Prokofeva, Yohei Ohashi, Andre Horatscheck, Davide Cirillo, Michael Krauss, Christopher Schmied, Martin Neuenschwander, Jens Peter von Kries, Guillaume Medard, Bernhard Kuster, Olga Perisic, Roger L. Williams, Oliver Daumke, Bernard Payrastre, Sonia Severin, Marc Nazare, Volker Haucke
Summary: In this study, we developed a novel PI3KC2 alpha catalytic activity inhibitor PITCOIN, which exhibits strong selectivity towards PI3KC2 alpha due to its unique mode of interaction with the ATP-binding site of the enzyme. It was demonstrated that PITCOINs can inhibit the PI3KC2 alpha-mediated synthesis of phosphatidylinositol 3-phosphates, impairing endocytic membrane dynamics and platelet-dependent thrombus formation.
NATURE CHEMICAL BIOLOGY
(2023)
Article
Biology
Agnes Ribes, Cedric Garcia, Marie-Pierre Gratacap, Evi Kostenis, Laurent O. Martinez, Bernard Payrastre, Jean-Michel Senard, Celine Gales, Veronique Pons
Summary: This study investigated the pharmacology of P2Y(1)-R signaling in various cells and platelets and found that P2Y(1)-R is constitutively active in physiological conditions. The study also identified P2Y(1)-R antagonists that can inhibit this constitutive activation. These findings suggest that targeting P2Y(1)-R constitutive activation could be a promising strategy for antiplatelet therapy.
Article
Cell Biology
Waltraud Cornelia Schrottmaier, Julia Barbara Kral-Pointner, Manuel Salzmann, Marion Mussbacher, Anna Schmuckenschlager, Anita Pirabe, Laura Brunnthaler, Mario Kuttke, Barbara Maier, Stefan Heber, Hannes Datler, Yasemin Ekici, Birgit Niederreiter, Ulrike Heber, Bo Blomgren, Anna-Dorothea Gorki, Cecilia Soederberg-Naucler, Bernard Payrastre, Marie-Pierre Gratacap, Sylvia Knapp, Gernot Schabbauer, Alice Assinger
Summary: Phosphatidylinositol 3-kinase catalytic subunit p110 beta plays a role in tumorigenesis and hemostasis. This study investigates the role of p110 beta in platelet-mediated immune responses and finds that its dysfunction exacerbates infection by impeding leukocyte functions. These findings provide important insights into the immunomodulatory potential of PI3K inhibitors in bacterial infection.
Article
Biochemistry & Molecular Biology
Abdulrahman Mujalli, Julien Viaud, Sonia Severin, Marie-Pierre Gratacap, Gaetan Chicanne, Karim Hnia, Bernard Payrastre, Anne-Dominique Terrisse
Summary: Phosphoinositides (PIs) are important for regulating intracellular signaling and membrane trafficking. This study reveals a previously unknown pool of phosphatidylinositol 3-monophosphate (PI3P) in the outer leaflet of resting platelet plasma membrane. The presence of external PI3P is influenced by certain kinases and can bind to PI3P-binding proteins, suggesting a potential role in platelet communication and protein elimination.
Article
Hematology
Caroline Kardeby, Alice Evans, Joana Campos, Afraa Moosa Al-Wahaibi, Christopher W. Smith, Alexandre Slater, Eleyna M. Martin, Sonia Severin, Alexander Brill, Gunnar Pejler, Yi Sun, Steve P. Watson
Summary: English Summary: The study identified PEAR1 as a receptor for heparin and HPGM, and PI3K(3 as a key signaling molecule downstream of PEAR1 in platelets. These findings may provide important insights into the role of PEAR1 in cardiovascular disease.
JOURNAL OF THROMBOSIS AND HAEMOSTASIS
(2023)
Article
Biochemistry & Molecular Biology
Sonia Severin, Alessandra Consonni, Gaetan Chicanne, Sophie Allart, Bernard Payrastre, Marie-Pierre Gratacap
Summary: In this study, the role of SHIP1 in platelet activation was investigated. SHIP1 was found to regulate cytoskeletal organization and internal contractile activity in thrombin-activated platelets, and it interacted with the spectrin-based membrane skeleton. The absence of SHIP1 resulted in a loss of sustained association of integrins with the membrane skeleton and a decrease in α(IIb)β(3) integrin clustering.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
