Journal
JOURNAL OF THROMBOSIS AND HAEMOSTASIS
Volume 9, Issue 12, Pages 2447-2456Publisher
WILEY
DOI: 10.1111/j.1538-7836.2011.04532.x
Keywords
antiphospholipid syndrome; autoantibodies; ss 2-glycoprotein I
Categories
Funding
- Netherlands Organization for Scientific Research (ZonMW) [91207002]
- Swedish Research Council [7480]
- AMC
Ask authors/readers for more resources
Background: The antiphospholipid syndrome (APS) is characterized by the persistent presence of anti-beta 2-glycoprotein I (beta 2-GPI) autoantibodies. beta 2-GPI can exist in two conformations. In plasma it is a circular protein, whereas it adopts a fish-hook conformation after binding to phospholipids. Only the latter conformation is recognized by patient antibodies. beta 2-GPI has been shown to interact with Streptococcus pyogenes. Objective: To evaluate the potential of S. pyogenes-derived proteins to induce anti-beta 2-GPI autoantibodies. Methods and results: Four S. pyogenes surface proteins (M1 protein, protein H, streptococcal collagen-like protein A [SclA], and streptococcal collagen-like protein B [SclB]) were found to interact with beta 2-GPI. Only binding to protein H induces a conformational change in beta 2-GPI, thereby exposing a cryptic epitope for APS-related autoantibodies. Mice were injected with the four proteins. Only mice injected with protein H developed antibodies against the patient antibody-related epitope in domain I of beta 2-GPI. Patients with pharyngotonsillitis caused by S. pyogenes who developed anti-protein H antibodies also generated anti-beta 2-GPI antibodies. Conclusions: Our study has demonstrated that a bacterial protein can induce a conformational change in beta 2-GPI, resulting in the formation of anti beta 2-GPI autoantibodies. This constitutes a novel mechanism for the formation of anti-beta 2-GPI autoantibodies.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available