Article
Green & Sustainable Science & Technology
Zahra Shokravi, Hoofar Shokravi, A. E. Atabani, Woei Jye Lau, Ong Hwai Chyuan, Ahmad Fauzi Ismail
Summary: This paper provides a compilation of technical developments aimed at promoting fatty acid profile (FAP) and lipid yield (LY) during the harvesting process of microalgae. Co-cultivating specific microalgae with fungi, bacteria, or other microalgae strains was found to improve flocculation efficiency and FAP&LY. Proper wet storage conditions and stress induction strategies could enhance FAP&LY efficiency and reduce expenses associated with cell drying and related equipment. Enhancing FAP&LY through the harvesting process could be a breakthrough technology for cost-efficient microalgaebased biodiesel production.
RENEWABLE & SUSTAINABLE ENERGY REVIEWS
(2022)
Review
Fisheries
Jose-Pedro Canavate, Catalina Fernandez-Diaz
Summary: Salinity is a fundamental factor in microalgal aquaculture production, affecting the lipid composition and production of microalgae. Different microalgal species show varying responses to salinity, with hypersalinity having a greater impact on lipid profiles in cultured marine microalgae. Optimizing salinity levels can lead to more sustainable microalgal production.
REVIEWS IN AQUACULTURE
(2022)
Article
Chemistry, Multidisciplinary
Saumita Chakravarty, Nirupama Mallick
Summary: Microalgal-derived biofuels play a significant role in sustainable and green energy generation. This study aims to enhance lipid yields and quality, reduce experimental time and chemical usage, and increase cost-effectiveness and mass applicability. Through optimizing macronutrient stresses, an optimized medium was developed, resulting in higher lipid content and productivity, reduced chemical requirements, and lower medium cost. The optimized medium showed promising annual outcomes and suitability for different microalgae strains.
SUSTAINABLE CHEMISTRY AND PHARMACY
(2022)
Article
Agricultural Engineering
Anil Kumar Patel, Ajeet Singh Chauhan, Prashant Kumar, Philippe Michaud, Vijai Kumar Gupta, Jo-Shu Chang, Chiu-Wen Chen, Cheng-Di Dong, Reeta Rani Singhania
Summary: This article provides an update on the production of essential fatty acids from potential microbes and explores novel strategies to promote the accumulation of omega-3 and omega-6 through engineering and omics approaches.
BIORESOURCE TECHNOLOGY
(2022)
Article
Environmental Sciences
Gang Li, Jiang Zhang, Huan Li, Ruichen Hu, Xiaolong Yao, Ying Liu, Yuguang Zhou, Tao Lyu
Summary: This study focused on the simultaneous treatment of livestock wastewater and high-quality biodiesel production using microalgae technology. Higher microalgal biomass production was observed in the original piggery effluent medium, while microalgae grown in anaerobically-digested effluent had a higher lipid content, which is favorable for biodiesel production. The N/P ratio in the original effluent was closer to the optimal ratio for microalgae growth compared to the anaerobically-digested effluent, indicating potential for sustainable livestock waste management.
Article
Green & Sustainable Science & Technology
Zi-Hao Qin, Xiaomeng Hu, Jin-Hua Mou, Guo-Hui He, Guang-Bin Ye, Hong-Ye Li, Shauhrat Singh Chopra, Liang Dong, Carol Sze Ki Lin, Xiang Wang
Summary: This study conducted Life Cycle Assessments (LCA) to evaluate the environmental impact of laboratory-scale EPA production by Phaeodactylum tricornutum. The results showed that technical advancements, particularly metabolic engineering, significantly reduced the global warming potential and cumulative energy demand associated with EPA production. Electricity consumption and extraction solvents were identified as the main contributors to the environmental impact. The study suggests future directions should focus on increasing EPA productivity, adopting alternative cultivation approaches with less energy input, and implementing environmentally friendly methods for EPA recovery.
JOURNAL OF CLEANER PRODUCTION
(2023)
Article
Plant Sciences
Meng-meng Huang, Xing Xin, Hui-ling Shi, Yi-meng Lin, Hong-zhi Ling, Jingping Ge
Summary: This study isolated six microalgae strains from alpine regions and evaluated their potential for biodiesel and high-value compound production. Among them, Chlorella sp. HDA04 showed potential as a biodiesel producer, while Chlorella sp. HDA07 was suitable for high-value compound production.
SOUTH AFRICAN JOURNAL OF BOTANY
(2022)
Review
Biotechnology & Applied Microbiology
Bayu Afnovandra Perdana, Zulkarnain Chaidir, Arif Juliari Kusnanda, Abdi Dharma, Indra Junaidi Zakaria, Syafrizayanti, Asep Bayu, Masteria Yunovilsa Putra
Summary: Microalgae are a promising source of omega-3 fatty acids, and their production can be enhanced by improving exogenous factors and optimizing cultivation conditions. Adjusting factors such as temperature, nutrients, and light can increase lipid content and omega-3 fatty acid levels in microalgae.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2021)
Article
Agricultural Engineering
M. Mofijur, S. M. Ashrafur Rahman, Luong N. Nguyen, T. M. Mahlia, L. D. Nghiem
Summary: The study developed and applied the PROMETHEE-GAIA method to select microalgae strains for aviation fuel production, with Chlorella sp. NT8a identified as the most suitable strain. However, unmodified biofuel from the chosen strain did not meet all jet fuel standards, highlighting the need for processing or modification of biofuels and revision of jet fuel standards to introduce microalgae-based biofuels into the aviation industry.
BIORESOURCE TECHNOLOGY
(2022)
Article
Energy & Fuels
Najmeh Hamidian, Hajar Zamani
Summary: The study investigated the cultivation of freshwater microalgae Chlorella sorokiniana EAKI in untreated dairy wastewater as a low-cost nutrient source for biofuel production. Undiluted wastewater was found to result in the highest microalgae biomass production and lipid accumulation, making it a cost-effective option for sustainable biofuel generation. The biodiesel produced from wastewater-grown C. sorokiniana showed promising physico-chemical characteristics, confirming its potential for high-quality biodiesel production.
BIOENERGY RESEARCH
(2022)
Article
Agricultural Engineering
Bancha Kumsiri, Jeeraporn Pekkoh, Wasu Pathom-aree, Saisamorn Lumyong, Kittiya Phinyo, Chayakorn Pumas, Sirasit Srinuanpan
Summary: The study proposes an innovative approach using actinomycetes co-culture with microalgae in biogas digestate effluent to enhance biomass and lipid production, providing a sustainable feedstock for biodiesel. The co-cultivation significantly increased biomass production, chlorophyll a content, and lipid productivity compared to microalgae monoculture, with accumulated lipids containing long-chain fatty acids suitable for biodiesel production. Overall, the actinomycete co-culture shows great potential for cost-effective and environmentally friendly microbial-based biofuel production.
BIORESOURCE TECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Akash Anandraj, Sarah White, Joseph K. Bwapwa
Summary: Temperature and nutrient conditions affect the physiology and FAME composition of Chlorella sp., with dual stress leading to increased lipid yield. Identified FAMEs C-16 and C-18 have potential for quality biodiesel and aviation fuel.
BIOFUELS BIOPRODUCTS & BIOREFINING-BIOFPR
(2021)
Article
Biotechnology & Applied Microbiology
Wenjia Gu, John M. Kavanagh, Dale D. McClure
Summary: A kinetic model for EPA-producing microalga Phaeodactylum tricornutum was developed and successfully validated, demonstrating its potential for large-scale EPA production.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Zhengshuai Wu, Shuang Qiu, Abdul-Wahab Abbew, Zhipeng Chen, Yanlin Liu, Jinyu Zuo, Shijian Ge
Summary: Microalgae-based wastewater treatment offers a competitive alternative to traditional biological techniques. This study investigated the effects of nitrogen sources, levels, and feeding modes on microalgal biomass, nitrogen removal, and the co-production of fucoxanthin and fatty acids. Urea supplementation achieved over 90% nitrogen removal efficiency and enhanced microalgal growth and fatty acid accumulation. With 12.35 mg-N/L urea, the co-production of fucoxanthin and fatty acids was optimized with one-time feeding on D0 and two-time feedings on D0 and D5.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2022)
Review
Biotechnology & Applied Microbiology
Wenjia Gu, John M. Kavanagh, Dale D. McClure
Summary: Eicosapentaenoic acid (EPA) is an essential omega-3 fatty acid for both humans and animals, with a growing global demand for it. Alternative sources such as heterotrophic production and genetically modified organisms have been explored to address potential supply shortages. Widely used species for large-scale EPA production include Phaeodactylum tricornutum and Nannochloropsis, with considerations on economic factors and sustainability.
CRITICAL REVIEWS IN BIOTECHNOLOGY
(2021)
Article
Energy & Fuels
Najwa Mlaik, Sami Sayadi, Nesrine Mnasri, Sonia Kechaou, Salim Loukil, Fathi Aloui, Sonia Khoufi
Summary: This study investigated the dry anaerobic co-digestion of vegetable wastes and animal manures. The results showed that co-digestion significantly increased methane yield and improved the quality of the digestate.
BIOMASS CONVERSION AND BIOREFINERY
(2023)
Article
Energy & Fuels
Ines Dahmen-Ben Moussa, Mohamed Ali Masmoudi, Sirine Choura, Mohamed Chamkha, Sami Sayadi
Summary: This study optimized the extraction of polyphenols from microalgae and found that Chlorella showed the best extraction results at methanol, 80 degrees C, and 4 hours. It was also discovered that polyphenols extracted from microalgae grown with olive mill wastewater exhibited strong antioxidant and antimicrobial activities.
BIOMASS CONVERSION AND BIOREFINERY
(2023)
Correction
Chemistry, Physical
Sonia Ben Younes, Saoussen Ben Khedher, Yongjun Zhang, Sven-Uwe Geissen, Sami Sayadi
Article
Environmental Sciences
Olfa Baccari, Mohamed Barkallah, Jihen Elleuch, Nourelhouda Ben Ayed, Amel Chtourou, Hela Karray-Hakim, Adenene Hammami, Philippe Michaud, Imen Fendri, Slim Abdelkafi
Summary: Parachlamydia acanthamoebae, a potential respiratory pathogen, was detected using a TaqMan-PCR assay that showed good accuracy and reproducibility. This diagnostic tool can be used for large-scale screening and during outbreaks.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Article
Biochemical Research Methods
Olfa Baccari, Mohamed Barkallah, Jihen Elleuch, Nourelhouda Ben Ayed, Amel Chtourou, Hela Karray-Hakim, Adenene Hammami, Philippe Michaud, Imen Fendri, Slim Abdelkafi
Summary: This study developed a new duplex probe-based q-PCR assay for simultaneous detection and quantification of Parachlamydia acanthamoebae and Simkania negevensis. The assay showed high accuracy and sensitivity in detecting these bacteria in water and clinical samples.
ANALYTICAL BIOCHEMISTRY
(2023)
Article
Food Science & Technology
Ali Ellafi, Racha Farhat, Mejdi Snoussi, Emira Noumi, El Hassane Anouar, Ridha Ben Ali, Michele Veronique El May, Sami Sayadi, Kaiss Aouadi, Adel Kadri, Sonia Ben Younes
Summary: The phytochemical variability of Juglans regia root aqueous extract (JRRAE) was investigated, revealing the presence of biomolecules. The extract showed significant antibacterial activity, inhibited biofilm formation, induced lethality in Leishmania amazonensis promastigotes, and exhibited insecticidal activity. Molecular docking studies confirmed that the activities were mainly due to specific compounds present in the extract.
INTERNATIONAL JOURNAL OF FOOD PROPERTIES
(2023)
Article
Chemistry, Medicinal
Meriem Aitouguinane, Zainab El Alaoui-Talibi, Halima Rchid, Imen Fendri, Slim Abdelkafi, Mohamed Didi Ould El-Hadj, Zakaria Boual, Didier Le Cerf, Christophe Rihouey, Christine Gardarin, Pascal Dubessay, Philippe Michaud, Guillaume Pierre, Cedric Delattre, Cherkaoui El Modafar
Summary: The study investigated the ability of alginates extracted from two Moroccan brown seaweeds and their derivatives to induce phenolic metabolism in tomato seedlings. Elicitation was done by foliar spraying the seaweed alginates on tomato seedlings and evaluating their effects on PAL activity, polyphenol, and lignin production. It was found that low-molecular-weight alginates were more effective in inducing natural defenses in tomato seedlings compared to the alginate polymers. These findings suggest that low-molecular-weight alginates may have potential as a stimulant for plant natural defenses.
Article
Chemistry, Multidisciplinary
Hana Derbel, Jihen Elleuch, Wael Mahfoudh, Philippe Michaud, Imen Fendri, Slim Abdelkafi
Summary: Bioactive peptides extracted from microalgae have gained significant attention recently due to their promising properties. In this study, proteins from the marine microalga Rhodomonas sp. were hydrolyzed into peptides using alcalase and pepsin. The biological activities of the protein preparations were evaluated and it was found that the crude protein exhibited higher antioxidant and anti-inflammatory activities compared to the peptides obtained after hydrolysis.
APPLIED SCIENCES-BASEL
(2023)
Review
Microbiology
Fatma Masmoudi, Mohammed Alsafran, Hareb AL Jabri, Hoda Hosseini, Mohammed Trigui, Sami Sayadi, Slim Tounsi, Imen Saadaoui
Summary: Abiotic and biotic stresses have negative impacts on plant growth and crop yield. Traditional methods for managing stress factors have shown limited success. Halotolerant bacteria have emerged as potential plant promoters under stressful conditions. This review examines the capability of plant-growth-promoting halobacteria (PGPH) to enhance plant growth, improve tolerance to stressors, and sustain soil fertility. The review also discusses the limitations and concerns of using PGPH as an innovative approach for boosting crop production and food security.
Review
Chemistry, Medicinal
Latifa Tounsi, Hajer Ben Hlima, Faiez Hentati, Ons Hentati, Hana Derbel, Philippe Michaud, Slim Abdelkafi
Summary: This review provides a detailed overview of optimizing culture media elements and physical parameters to improve the large-scale production of phycobiliproteins. It also explores the main features of phycobiliproteins and examines their potential for biotechnological uses in various fields, with a focus on health and nutritional claims.
Article
Environmental Sciences
Aziza Safaa Chenine, Zakaria Boual, Mohamed Didi Ould Elhadj, Noura Addoun, Reguia Mahfoudi, Aicha Khemili, Hakim Belkhalfa, Khaldoun Bachari, Imen Fendri, Cherkaoui El Modafar, Zainab Alaoui Talibi, Pascal Dubessay, Cedric Delattre, Guillaume Pierre, Philippe Michaud, Jihen Elleuch, Slim Abdelkafi
Summary: This study investigates the impact of partial acid hydrolysis on the antihyperglycemic activity of a polysaccharide derived from Plantago ciliata seeds. The results demonstrate that the resulting oligosaccharides exhibit significant inhibitory effects on alpha-amylase and alpha-D-glucosidase enzymes and act as non-competitive inhibitors. Additionally, these oligosaccharides show resistance to digestion, suggesting their potential as a natural and healthy drug for reducing blood sugar levels.
EURO-MEDITERRANEAN JOURNAL FOR ENVIRONMENTAL INTEGRATION
(2023)
Review
Green & Sustainable Science & Technology
Ilyes Dammak, Mariem Fersi, Ridha Hachicha, Slim Abdelkafi
Summary: Microalgae cultivation in municipal wastewater offers potential for pollution removal and biomass utilization, promoting environmental sustainability. However, scaling these systems to industrial levels presents challenges in maintaining consistent control over multiple influencing factors. The use of native microalgal consortiums appears to be a promising strategy, but further exploration of their potential is necessary.
Article
Environmental Sciences
Hakima Gharbi-Khelifi, Habib Jmii, Mohamed Mosbahi, Samiha Hamdi, Rawand Hamdi, Jihen Brahmi, Slim Loukil, Mohamed Chamkha, Sami Sayadi, Mahjoub Aouni, Ana Barreiro, Maria J. Fernandez-Sanjurjo, Avelino Nunez-Delgado, Esperanza Alvarez Rodriguez
Summary: This study evaluates the quality of treated wastewaters from two wastewater treatment plants in Tunisia and assesses the ability of local clays to enhance the wastewater quality. The results show that the registered values of microbiological and physicochemical parameters do not comply with the standards. However, the treatment process improves the microbiological parameters, especially with the use of specific clays.
ENVIRONMENTAL RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Farah Hadjkacem, Jihen Elleuch, Meriem Aitouguinane, Fatma Zohra Chakou, Alina Violeta Ursu, Pascal Dubessay, Nathalie Bourgougnon, Mounir Traikia, Didier Le Cerf, Zainab El Alaoui-Talibi, Cherkaoui El Modafar, Zakaria Boual, Mohamed Didi Ould El Hadj, Cedric Delattre, Gwendoline Christophe, Philippe Michaud, Imen Fendri, Slim Abdelkafi, Guillaume Pierre
Summary: This study evaluates the biological and biotechnological potentials of two water soluble polysaccharides (alginate and fucoidan) extracted from a Tunisian brown macroalgae. The polysaccharides showed significant anti-inflammatory, anticoagulant, antihyperglycemic, and antiviral activities, as well as the ability to activate the natural defenses of tomato seedlings.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Engineering, Chemical
Shifa M. R. Shaikh, Mohammad Abdul Quadir, Mustafa S. Nasser, Hamza Rekik, Mohammad K. Hassan, Ahmad I. Ayesh, Sami Sayadi
Summary: This study demonstrates that coagulation-flocculation techniques can effectively achieve flocculation of freshwater microalgae Scenedesmus sp. cultivated using process wastewater, and exhibits non-Newtonian fluid behavior and viscoelastic gel properties.
SEPARATION AND PURIFICATION TECHNOLOGY
(2024)
