Protein molecular structures in alfalfa hay cut at three stages of maturity and in the afternoon and morning and relationship with nutrient availability in ruminants

BACKGROUND: Molecular structures in feed protein influence its digestive behavior, availability andutilization. From a nutritive point of view, stage of maturity and cutting time are important factors affecting nutrient profiles and availability of alfalfa (Medicago sativa L.) hay in ruminants. The objectives of this study were to determine protein molecular structures by Fourier transform infrared spectroscopy (FTIR), and their relationship with nutrient profiles and availability in ruminants of alfalfa hay cut at early bud, late bud and early flower stages and in afternoon and morning. RESULTS: With advancing maturity, molecular structure ratios of alpha-helix: beta-sheets decreased, while amide I: amide II increased (P <= 0.05). Alfalfa cutting in afternoon versus morning increased protein structure alpha-helix:beta-sheets and alpha-helix:others ratios (P < 0.05) and tended to decrease the proportion of beta-sheets (P = 0.09). Positive correlations were found for alpha-helix:beta-sheet ratio (R >= 0.60; P < 0.05) with intermediately degradable protein (PB2) and ruminal degradability and intestinal protein supply, and all these parameters correlated negatively with amide I: amide II ratio (R <= -0.62; P < 0.05). CONCLUSION: Protein molecular structures in alfalfa hay changed with advancing maturity and during the day and these protein structures affected predicted nutrient availability of alfalfa hay in ruminants. (C) 2013 Society of Chemical Industry