Article
Chemistry, Applied
Fanfan Yang, Long Xu, Alberto C. P. Dias, Xiaoying Zhang
Summary: The competitive sandwich immunoassay offers a sensitive and accurate method for detecting histamine in seafood, with a wide linear range and low detection limit.
Review
Food Science & Technology
Xin Dong, Vijaya Raghavan
Summary: Seafood is a nutritious food that plays a significant role in human health, but seafood allergy has become a global health issue. This review provides an overview of recent advances in processing techniques and allergen-detection methods for seafood products, aiming to provide key information for further research.
COMPREHENSIVE REVIEWS IN FOOD SCIENCE AND FOOD SAFETY
(2022)
Article
Biotechnology & Applied Microbiology
Yang Mu, Cunyu Jia, Xu Zheng, Haipeng Zhu, Xin Zhang, Haoran Xu, Baoyuan Liu, Qin Zhao, En-Min Zhou
Summary: A rapid, low-cost, reliable and useful cELISA method based on nanobody technology was developed for detecting PCV2 antibodies. The experiment demonstrated that this method has high sensitivity and specificity, and a high consistency rate with commercial kits.
JOURNAL OF NANOBIOTECHNOLOGY
(2021)
Review
Food Science & Technology
Regina Klapper, Horst Karl, Ute Ostermeyer
Summary: Efforts are being made to identify anisakid nematode larvae in fish products to estimate health risks and develop effective detection methods. Understanding the structural properties of the larvae and compounds produced during host-parasite interactions is crucial for improved detection. Various detection methods, including anatomy, compounds, and fluorescence characteristics, are being explored in both the medical field and fish processing industries for detecting anisakid larvae.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2021)
Article
Biochemical Research Methods
Shaolong Feng, Marti Z. Hua, M. S. Roopesh, Xiaonan Lu
Summary: We have developed a competitive ELISA-based origami microfluidic paper-based analytical device (mu PAD) for the detection of mycotoxins in animal feed materials. The mu PAD was patterned using the wax printing technique, with a testing pad in the middle and absorption pads on the sides. By immobilizing anti-mycotoxin antibodies on chitosan-glutaraldehyde-modified sample reservoirs, we successfully achieved the quantification of zearalenone, deoxynivalenol, and T-2 toxin in corn flour in just 20 minutes. The mu PAD integrated with competitive ELISA shows potential for rapid, sensitive, and cost-effective detection of various mycotoxins in animal feed materials, which is important for the livestock industry.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Microbiology
Xiuqin Chen, Lianjing Zhao, Jiahui Wang, Haolu Wang, Yangyuan Qiu, Zijian Dong, Chunling Zhang, Mingyuan Liu, Xuelin Wang, Xue Bai
Summary: In this study, a novel RPA-SG assay was developed for the rapid and specific detection of anisakids. The assay showed similar sensitivity to qPCR and high precision in detecting fresh marine fish contaminated with anisakids. This method has potential applications in field detection and food safety control.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Chemistry, Applied
Yifang Cui, Huiwen Wang, Fangfang Guo, Xiaoya Cao, Xue Wang, Ximin Zeng, Guolin Cui, Jun Lin, Fuzhou Xu
Summary: This study developed a monoclonal antibody-based ELISA method for quantifying Enterobacteriaceae levels in the intestine, which can be used to assess gut inflammation. The ELISA showed high accuracy, repeatability, and reproducibility, and was able to detect Enterobacteriaceae levels produced in different bacterial cultures.
Article
Chemistry, Analytical
Kui Gu, Peng Ma, Zengxu Song, Ming Yang, Xue Yang, Chao Li, Changyu Zhou, Zijing Ju, Yu Zhao, Hao Li, Xin Yang, Changwei Lei, Hongning Wang
Summary: African swine fever (ASF) is a highly contagious and lethal hemorrhagic disease, causing significant distress and economic losses to the world pig industry. The lack of approved vaccines and effective treatment makes accurate early detection of ASFV antigen and antibody crucial in preventing the outbreak and spread of ASFV. In this study, specific nanobodies against the ASFV-P30 protein were screened from an immunized Bactrian camel library and fused with horseradish peroxidase (HRP) for expression in HEK293T cells. A novel competitive ELISA (cELISA) based on ferritin-displayed P30 nanoparticles protein (P30-Fn) and nanobody-horseradish peroxidase fusions (P30-Nb71-vHRP) was developed for rapid and sensitive detection of ASFV antibody in serum samples, showing high sensitivity and specificity.
Correction
Biotechnology & Applied Microbiology
Yang Mu, Cunyu Jia, Xu Zheng, Haipeng Zhu, Xin Zhang, Haoran Xu, Baoyuan Liu, Qin Zhao, En-Min Zhou
Summary: A correction to this paper has been published and is accessible through the original article.
JOURNAL OF NANOBIOTECHNOLOGY
(2021)
Article
Food Science & Technology
Shokoofeh Shamsi, Jaydipbhai Suthar, Xiaocheng Zhu, Diane P. Barton
Summary: This study aimed to assess the occurrence and prevalence of infectious gnathostomatid parasites in selected commercial fish species in Australia. The study found that 12.3% of the fish were infected with at least one gnathostomatid larva, with the yellow-dabbled flounder exhibiting the highest prevalence. These findings are important for revising current seafood safety protocols in Australia.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2023)
Article
Agriculture, Dairy & Animal Science
Tianxiang Chen, Baoyuan Liu, Yiyang Chen, Xueting Wang, Meimei Zhang, Xukun Dang, Qin Zhao, En-Min Zhou
Summary: A rapid and reliable competitive ELISA (cELISA) was developed to detect avian HEV antibodies, which showed good specificity. The epitope recognized by Nb49 was located in aa 593-604 of the avian HEV capsid protein, and the peptide TFPS in aa 601-604 was essential for binding.
Article
Chemistry, Analytical
Yuan Cheng, Wenliang Ge, Hua Kuang, Jianping Zhu, Liqiang Liu, Yingyue Zhu, Chuanlai Xu
Summary: A highly sensitive and specific monoclonal antibody was developed for KCZ detection in chicken samples, with a visual limit of detection of 0.5 ng/g. The CGIA method shows great potential for rapidly detecting KCZ residues in chicken samples, with results revealed in only 10 minutes.
MICROCHEMICAL JOURNAL
(2022)
Article
Chemistry, Analytical
Kavya L. Singampalli, Jiran Li, Peter B. Lillehoj
Summary: Protein-based diagnostics are widely used for screening and diagnosing various diseases. The current gold standard method, enzyme-linked immunosorbent assay (ELISA), has high sensitivity but is time-consuming. This study presents a unique magneto-ELISA using labeled magnetic nanoparticles for rapid enrichment and signal amplification. The assay showed superior diagnostic accuracy in detecting malaria parasite biomarker and SARS-CoV-2 nucleocapsid protein, with fast completion time and compatibility with standard equipment.
ANALYTICA CHIMICA ACTA
(2022)
Article
Food Science & Technology
Yaozhou Zhu, Yun-Hwa Peggy Hsieh
Summary: This study successfully detected a wide spectrum of commercially important fish species using three specific monoclonal antibodies in the indirect enzyme-linked immunosorbent assay, exhibiting high sensitivity, specificity, and reproducibility.
Article
Biotechnology & Applied Microbiology
Ann-Katrin Llarena, Eystein Skjerve, Solfrid Bjorkoy, Merete Forseth, Julianne Winge, Sigrun J. Hauge, Gro S. Johannessen, Bjorn Spilsberg, Gunvor Elise Nagel-Alne
Summary: Campylobacter remains a major cause of bacterial gastroenteritis in Europe, with poultry, especially broiler chickens, being an important reservoir. Evaluation of four rapid detection methods showed that Biofire (R) FilmArray (R) had superior sensitivity and specificity compared to others, while the VIDAS Campylobacter, Singlepath (R) Campylobacter, and isothermal DNA amplification methods had lower sensitivity and were therefore unsuitable for monitoring Campylobacter in chickens. Further research to develop reliable methods for rapid detection of Campylobacter in chickens close to slaughter is needed.