Article
Horticulture
Tilen Zamljen, Aljaz Medic, Metka Hudina, Robert Veberic, Ana Slatnar
Summary: The effects of applying a biostimulant of hydrolyzed animal protein on the activity of key enzymes and capsaicinoid content in pepper plants under salt stress conditions were studied. The application of the biostimulant significantly increased the activity of capsaicin synthase (CS) and phenylalanine ammonia lyase (PAL), while decreasing peroxidase (POX) activity. Capsaicinoid content showed a strong correlation with the biostimulant application and salinity.
SCIENTIA HORTICULTURAE
(2023)
Article
Plant Sciences
Shan Jin, Naixing Ye, Xuejin Chen, Pengjie Wang, Mengya Gu, Binghao Hou, Churan Zhang, Yucheng Zheng, Yun Sun
Summary: This study characterized the PAL gene family in tea plants and investigated the role of CsPALs in anthocyanins accumulation. The analysis identified seven CsPAL genes and revealed that CsPAL4 plays a key role in the accumulation of different anthocyanins.
HORTICULTURAL PLANT JOURNAL
(2022)
Article
Biochemistry & Molecular Biology
Yu Qin, Qiue Li, Qiuju An, Dexin Li, Sipei Huang, Yongyang Zhao, Weijia Chen, Jiayu Zhou, Hai Liao
Summary: Drought is a key threat to plants and agriculture, and the role of the enzyme phenylalanine ammonia lyase (PAL) in drought tolerance has been explored in this study. A PAL member called FuPAL1 was isolated from the alpine pharmaceutical plant Fritillaria unibracteata. FuPAL1 was found to play a role in the ammonization process and was involved in substrate binding and catalysis. Transgenic Arabidopsis plants with FuPAL1 showed increased levels of lignin and salicylic acid and enhanced drought tolerance. These findings suggest that FuPAL1 could be a valuable target for improving drought resistance.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Biotechnology & Applied Microbiology
Feiyan Xue, Zihui Liu, Yue Yu, Yangjie Wu, Yuxin Jin, Mingfeng Yang, Lanqing Ma
Summary: Researchers cloned the cDNA of Rhodotorula glutinis PAL (RgPAL) and introduced codon optimization to enhance its protein expression and enzyme activity in Escherichia coli. This improvement allowed for the successful expression and purification of the recombinant enzyme PAL-opt, which exhibited high specific activity for substrate L-phenylalanine and L-tyrosine. This bifunctional enzyme provides a promising foundation for future applications.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Plant Sciences
Hassan S. Al-Zahrani, Hesham E. Alharby, Khalid Rehman Hakeem, Reiaz Ul Rehman
Summary: The study found that the application of zinc can alleviate the negative effects of salt stress on plant growth to a certain extent by improving physiological and biochemical changes, including increased shoot and root length, enhanced chlorophyll and carotenoid contents, and increased enzymatic antioxidant activities.
Article
Biochemistry & Molecular Biology
Renu Bhardwaj, Sunil Pareek, J. Abraham Dominguez-Avila, Gustavo A. Gonzalez-Aguilar, Daniel Valero, Maria Serrano
Summary: Exogenous melatonin treatment improves cold resistance of mangoes, with 'Langra' mangoes showing the greatest benefit and 'Gulab Jamun' mangoes showing the least. The positive effect is associated with an increase in endogenous melatonin concentration, which prevents reactive oxygen accumulation and stimulates non-enzymatic antioxidants. 'Langra' mangoes also exhibit enhanced antioxidant activity and cold resistance enzyme performance.
Article
Chemistry, Physical
Chun-Yen Hsieh, Yi-Hao Huang, Hui-Hsuan Yeh, Pei-Yu Hong, Che-Jen Hsiao, Lu-Sheng Hsieh
Summary: Bambusa oldhamii BoPAL4 exhibits broad substrate specificity, with optimal reaction pH and temperature varying for different substrates. A major substrate specificity site mutant, BoPAL4-H123F, shows improved affinity for L-phenylalanine. BoPAL4 is confirmed to utilize L-phenylalanine, L-tyrosine, and L-DOPA as substrates.
Article
Biochemistry & Molecular Biology
Fei Ye, Xinbo Guo, Bo Li, Haiqiang Chen, Xiaoyan Qiao
Summary: Harvesting seasons have a significant impact on the physicochemical qualities of large-leaf variety black tea. Black tea made from Huangyu leaves exhibited better chemical qualities and sensory characteristics, with the spring processed leaves showing superior physicochemical qualities. These findings provide scientific guidance for the production of high-quality large-leaf variety black tea in South China.
Article
Plant Sciences
Guohui Li, Cheng Song, Muhammad Aamir Manzoor, Daoyuan Li, Yunpeng Cao, Yongping Cai
Summary: This study identified three PAL genes in the pear genome and revealed their significant impact on stone cell and lignin contents in pear fruits. Additionally, a transcription factor PbWLIM1 that regulates the transcription level of PbPAL2 was also discovered.
Article
Plant Sciences
Kazem Ghassemi-Golezani, Salar Farhangi-Abriz
Summary: Biochar reduces lignification and enhances nutrient uptake by plant roots. Addition of biochar promotes root growth, activity, and the absorption of essential elements, while reducing oxidative damages caused by toxic substances.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2023)
Article
Engineering, Environmental
Jialian Wei, Sisi Liao, Muzi Li, Bin Zhu, Hongcheng Wang, Lei Gu, Huayan Yin, Xuye Du
Summary: Cadmium is a toxic heavy metal that poses a serious threat to crop safety and human health. This study investigated the molecular mechanisms underlying the response of Aegilops tauschii, a common wheat donor, to cadmium stress. Key genes involved in cadmium stress response were identified, including AetSRG1, which played a crucial role in reducing cadmium accumulation and increasing antioxidant production. These findings provide new insights into the development of low cadmium wheat.
JOURNAL OF HAZARDOUS MATERIALS
(2022)
Article
Plant Sciences
AiLian Liu, Yue Zhu, YuHao Wang, TianYu Wang, ShuPing Zhao, Kai Feng, LiangJun Li, Peng Wu
Summary: In this study, PAL genes in E. ferox were identified and functionally analyzed. EfPAL1 and EfPAL2 were found to be key genes in flavonoid biosynthesis, and a regulatory network for EfPAL2 was established. These findings provide novel information for understanding the mechanism of flavonoid biosynthesis.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Food Science & Technology
Weiwei Wang, Ting Le, Wei Wang, Luting Yu, Lijuan Yang, Heyuan Jiang
Summary: This study analyzed the antioxidant activity and contents of various components in black tea, and found that catechins are the most important components determining the antioxidant activity of black tea.
Article
Food Science & Technology
Ayumi Ito, Emiko Yanase
Summary: This study used liquid chromatography and mass spectrometry to analyze the quantitative changes in compounds during black tea processing. The results showed that the catechin content significantly decreased during processing, while the variation in theaflavin content was negatively correlated with the catechin content. Twenty-three compounds containing known compounds and 45 compounds significantly increased during specific stages were identified.
FOOD RESEARCH INTERNATIONAL
(2022)
Article
Food Science & Technology
Qingping Ma, Mengyao Qin, Laichao Song, Haiwei Sun, Hong Zhang, Huanhuan Wu, Zhihong Ren, Hui Liu, Gang Duan, Yu Wang, Zhaotang Ding
Summary: This study elucidated the flavor formation mechanism of Changqing tea and found that the coordination of flavonoid biosynthesis and amino acids biosynthesis resulted in the special flavor of Changqing tea.