Journal
JOURNAL OF THE ROYAL SOCIETY INTERFACE
Volume 9, Issue 67, Pages 313-327Publisher
ROYAL SOC
DOI: 10.1098/rsif.2011.0278
Keywords
plasma SiOx:H nanocoating; surface wettability; cell adhesion/migration; FAK-Rho GTPases signalling pathways
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Funding
- National Natural Science Foundation of China [30970721, 10772127]
- Chinese Postdoctoral Science Foundation [20090461340, 201003702]
- China Medical Board [82-412]
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Vascular endothelial cell (EC) adhesion and migration are essential processes in re-endothelialization of implanted biomaterials. There is no clear relationship and mechanism between EC adhesion and migration behaviour on surfaces with varying wettabilities. As model substrates, plasma SiOx:H nanocoatings with well-controlled surface wettability (with water contact angles in the range of 98.5 +/- 2.3 degrees to 26.3 +/- 4.0 degrees) were used in this study to investigate the effects of surface wettability on cell adhesion/migration and associated protein expressions in FAK-Rho GTPases signalling pathways. It was found that EC adhesion/migration showed opposite behaviour on the hydrophilic and hydrophobic surfaces (i.e. hydrophobic surfaces promoted EC migration but were anti-adhesions). The number of adherent ECs showed a maximum on hydrophilic surfaces, while cells adhered to hydrophobic surfaces exhibited a tendency for cell migration. The focal adhesion kinase (FAK) inhibitor targeting the Y-397 site of FAK could significantly inhibit cell adhesion/migration, suggesting that EC adhesion and migration on surfaces with different wettabilities involve (p) FAK and its downstream signalling pathways. Western blot results suggested that the FAK-Rho GTPases signalling pathways were correlative to EC migration on hydrophobic plasma SiOx:H surfaces, but uncertain to hydrophilic surfaces. This work demonstrated that surface wettability could induce cellular behaviours that were associated with different cellular signalling events.
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