Article
Biophysics
Deepak Patil, Sushma Kumari, Kaushik Chatterjee
Summary: This study presents an innovative spin-coating setup to generate nanotopography on 3D-printed polymer scaffolds, which exhibit excellent bactericidal and osteogenic activities. The nanostructured PLA surface showed a high bactericidal effect on P. aeruginosa and S. aureus, while also supporting pre-osteoblast attachment and proliferation. This work has important implications for engineering next-generation 3D printed bioactive tissue scaffolds.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2023)
Article
Engineering, Biomedical
Xiaoyi Wu, Wenan Peng, Gufeng Liu, Shilei Wang, Bo Duan, Jian Yu, Hongye Yang, Cui Huang
Summary: This study introduces the concept of extrafibrillar demineralization into the construction of dentin-derived biomaterials for the first time. By selectively removing the apatite minerals outside the dentin collagen, an extrafibrillarly demineralized dentin matrix (EDM) is formed. The EDM exhibits unique nanotopography and bone-like mechanical properties, promoting cell adhesion, migration, and osteogenic differentiation in vitro as well as enhancing in vivo bone healing of rat calvarial defects. The outstanding osteogenic performance of EDM is related to the activation of the focal adhesion-cytoskeleton-nucleus mechanotransduction axis.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Biotechnology & Applied Microbiology
Daun Kim, Yonghyun Gwon, Sunho Park, Woochan Kim, Kwidug Yun, Jangho Kim
Summary: The study developed a polycaprolactone-based scaffold for bone regeneration with a soluble eggshell membrane protein coating and nanotopography structure, exhibiting flexibility and mechanical strength. In vitro experiments showed that the scaffold could regulate and enhance cell morphology, adhesion, proliferation, and differentiation.
BIOTECHNOLOGY AND BIOENGINEERING
(2021)
Review
Chemistry, Multidisciplinary
Arun Kumar Rajendran, Hwan D. D. Kim, Jung-Whan Kim, Jong Woo Bae, Nathaniel S. S. Hwang
Summary: Nanotechnology has achieved unprecedented progress in the field of biomaterials science. Researchers in tissue engineering and regenerative medicine are extensively utilizing nanotechnology-based systems for targeted and triggered drug delivery, as well as enhancing tissue regeneration by mimicking nature with nanoscale structures. Technical advancements, such as photolithography, have enabled the incorporation of nanoscale features into existing biomaterials for bench-to-bedside applications. The development of gel systems, magnetic nanoparticle controllable systems, and spheroid and organoid cultures has further expanded the field. This review provides an overview of the various nanotechnological approaches being explored and utilized to enhance tissue regeneration.
KOREAN JOURNAL OF CHEMICAL ENGINEERING
(2023)
Article
Materials Science, Multidisciplinary
Tao Xu, Xu Zhang, Xiu Dai
Summary: The construction of a suitable microenvironment for nerve regeneration is crucial for the functional recovery of defective nerves. In this study, PLA/COL hybrid fibrous membranes with a nanoporous structure on the fiber surface were fabricated, and their effects on neural cells were systematically investigated. The results showed that the membranes had appropriate mechanical properties, improved hydrophilicity, and good biocompatibility. The nanoporous structure and the introduction of COL promoted the proliferation and elongation of Schwann cells and the extension of dorsal root ganglion neurites.
MACROMOLECULAR MATERIALS AND ENGINEERING
(2022)
Article
Cell & Tissue Engineering
Robert E. Marx, Paul Amailuk, Neal Patel, Andre Ledoux, Dani Stanbouly
Summary: This study compared the effectiveness of three devices in harvesting stem/progenitor cells from bone marrow and found that a flexible bone marrow aspirator produced higher yields of stem/progenitor cells, resulting in greater bone regeneration. This highlights the importance of stem/progenitor cell yield in tissue engineering for bone regeneration.
JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE
(2022)
Article
Engineering, Biomedical
Naiana Suter, Sophie Stebel, Carmela Rianna, Manfred Radmacher, Dorothea Bruegemann
Summary: Current knowledge on cell-biomaterial interactions is largely based on 2D cell culture systems, but transferring results to 3D protein scaffolds with biomimetic nanofiber structures remains a challenge. By fabricating binary collagen scaffolds with variable thickness and topographies, researchers were able to observe differences in cell responses to different protein topographies, indicating potential for further understanding topography-dependent cell recognition processes.
Article
Cell & Tissue Engineering
Jing Li, Youming Zhu, Na Li, Tao Wu, Xianyu Zheng, Boon Chin Heng, Duohong Zou, Jianguang Xu
Summary: By overexpressing ETV2, human dental pulp stem cells (DPSCs) showed significantly enhanced differentiation into endothelial cells, with increased angiogenic potential both in vitro and in vivo. Proteomic analysis revealed upregulation of VEGF receptors, indicating enhanced VEGF signaling. These results suggest that DPSCs could be a promising cell source for tissue engineering applications.
CELL TRANSPLANTATION
(2021)
Article
Biochemistry & Molecular Biology
Tongtong Zhang, Juehong Li, Ziyang Sun, Bing Tu, Wei Wang, Gang Luo, Yunwei He, Shichao Jiang, Cunyi Fan
Summary: In this study, osteoprogenitors were successfully isolated from human heterotopic ossification tissues and identified for their strong osteogenic potential and gene transcriptions. The activation of ERK and hedgehog signaling pathways was shown to enhance the osteogenic potential of the osteoprogenitors.
Article
Biochemistry & Molecular Biology
Neus Gomila Pelegri, Aleksandra M. Stanczak, Amy L. Bottomley, Max L. Cummins, Bruce K. Milthorpe, Catherine A. Gorrie, Matthew P. Padula, Jerran Santos
Summary: This study cultured human adipose-derived stem cells (ADSCs) in 1.1 kPa polyethylene-glycol 3D hydrogels and examined the neural differentiation potential of ADSCs using B27, CultureOne (C1), and N2 neural supplements. The results showed that B27 and C1 enhanced the expression of neural markers in ADSCs, while N2 did not. Additionally, there were morphological changes observed in 2D environments but not in 3D environments.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Engineering, Biomedical
Rui Zhang, Junqun Jiang, Yaling Yu, Fangfang Wang, Niuniu Gao, Yingjie Zhou, Xinlong Wan, Zhibin Wang, Peng Wei, Jin Mei
Summary: Chronic kidney disease is a major global public health problem, with renal fibrosis being a common pathological process in chronic renal failure. Research indicates that renal fibrosis decellularized scaffolds have the potential to serve as a model for cellular mechanisms of tissue fibrosis or donors for tissue engineering. Analysis through histology, proteomics, and ELISA showed changes in the structure and components of fibrotic tissue. The fibrosis model induced by adenine-fed demonstrated changes in the fibrotic scaffold's structure and protein expression, but cytokines associated with renal regeneration post-injury were maintained, indicating potential for tissue engineering.
BIOACTIVE MATERIALS
(2021)
Article
Biochemistry & Molecular Biology
Neus Gomila Pelegri, Aleksandra M. M. Stanczak, Amy L. L. Bottomley, Bruce K. K. Milthorpe, Catherine A. A. Gorrie, Matthew P. P. Padula, Jerran Santos
Summary: Neurological diseases are a major cause of disability and death worldwide, but effective treatments are still difficult to develop. Tissue engineering provides a potential solution for testing treatments, but creating accurate brain tissue models remains challenging. This study focused on using adipose-derived stem cells (ADSCs) to create neural models and found that growing ADSCs in a 3D environment with similar mechanical properties to the central nervous system can promote spontaneous neural differentiation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Engineering, Biomedical
Stefanie Heltmann-Meyer, Dominik Steiner, Claudia Mueller, Dominik Schneidereit, Oliver Friedrich, Sahar Salehi, Felix B. Engel, Andreas Arkudas, Raymund E. Horch
Summary: The study showed that GelMA hydrogels are stable and biocompatible in the rat AVL model, promoting connective tissue formation and vascularization. Multiphoton microscopy was introduced as a new method to visualize neovessel formation.
BIOMEDICAL MATERIALS
(2021)
Article
Cell & Tissue Engineering
Alyssa Peterson, Lakshmi S. Nair
Summary: This review discusses the characteristics of hair follicle stem cells (HFSCs) and their potential applications in skin, bone, cardiovascular, and nerve tissue engineering.
TISSUE ENGINEERING PART B-REVIEWS
(2022)
Article
Engineering, Biomedical
Benjamin J. Bielajew, Ryan P. Donahue, Elliott K. Lamkin, Jerry C. Hu, Vincent C. Hascall, Kyriacos A. Athanasiou
Summary: In this study, proteomics, mechanical, and biochemical analyses were used to quantitatively investigate self-assembled neocartilage development from passaged, rejuvenated costal chondrocytes. The results showed that the temporal trends in neocartilage formation are similar to those seen in native hyaline articular cartilage development. Importantly, self-assembled neocartilage derived from costal chondrocytes is suitable for a non-homologous approach in the knee.
BIOMATERIALS RESEARCH
(2022)
Article
Endocrinology & Metabolism
Yang-Hee Kim, Richard O. C. Oreffo, Jonathan Dawson
Summary: In the past decade, there has been an increasing recognition of the role of the innate immune response in mediating repair and biomaterial directed tissue regeneration. The macrophage is now seen as a key player in orchestrating the resolution of inflammation and initiating the process of remodeling and repair. In the context of bone, macrophages have been found to have an essential coordinating role in supporting bone repair and facilitating biomaterial driven osteogenesis. This review examines the evidence for the role of macrophages in bone regeneration and repair, and discusses recent approaches that target macrophage modulation to accelerate and enhance bone tissue regeneration.
Article
Multidisciplinary Sciences
Hilary J. Anderson, Jugal Kishore Sahoo, Julia Wells, Sebastiaan van Nuffel, Hala S. Dhowre, Richard O. C. Oreffo, Mischa Zelzer, Rein V. Ulijn, Matthew J. Dalby
Summary: Skeletal stem cells are a dynamic population of cells that can enter quiescence, self-renew, or differentiate depending on regenerative demand and signals from their environment. This study presents an autonomous system where the cell-controlled surface can modulate cell adhesion and phenotype, demonstrating the potential for stem cell phenotype modulation.
SCIENTIFIC REPORTS
(2022)
Article
Materials Science, Multidisciplinary
Jack Perris, Charchit Kumar, Yang Xu, Manlio Tassieri, Mehmet E. Kartal, Nikolaj Gadegaard, Daniel M. Mulvihill
Summary: This study presents a method for rapidly producing high-quality polymer surface topographies from numerically generated surfaces using advanced surface generation tools. Injection molding provides high throughput and quality replication, while casting with low-viscosity resin enables slightly improved high-quality replication. The technology is important for researching surface phenomena sensitive to roughness.
ADVANCED ENGINEERING MATERIALS
(2022)
Article
Biochemistry & Molecular Biology
Estrela Neto, Luis Leitao, Jose C. Mateus, Daniela M. Sousa, Cecilia J. Alves, Miguel Aroso, Ana C. Monteiro, Francisco Conceicao, Richard O. C. Oreffo, Jonathan West, Paulo Aguiar, Meriem Lamghari
Summary: This study reveals that osteoclast-derived extracellular vesicles (EV) promote sensory neuron outgrowth in bone by targeting EGFR/ErbB2 signaling pathway. Additionally, osteoclasts also enhance sensory neurons' electrophysiological activity, potentially leading to nerve sensitization in the bone microenvironment.
CELL AND BIOSCIENCE
(2022)
Article
Nanoscience & Nanotechnology
Victor Tabouillot, Rahul Kumar, Paula L. Lalaguna, Maryam Hajji, Rebecca Clarke, Affar S. Karimullah, Andrew R. Thomson, Andrew Sutherland, Nikolaj Gadegaard, Shun Hashiyada, Malcolm Kadodwala
Summary: In this study, we harness the power of nanophotonic platforms and plasmonic circularly polarized luminescence (PCPL) to detect the chirality of monolayer quantities of a de novo designed peptide. This demonstrates that optimal biomolecular detection performance can be achieved by leveraging the near field sensitivity of PCPL, opening new avenues for nanometrology.
Editorial Material
Endocrinology & Metabolism
Jillian Cornish, Richard O. C. Oreffo
Article
Chemistry, Physical
Olga Guselnikova, Roman Elashnikov, Vaclav Svorcik, Martin Kartau, Cameron Gilroy, Nikolaj Gadegaard, Malcolm Kadodwala, Affar S. S. Karimullah, Oleksiy Lyutakov
Summary: The use of surface enhanced Raman scattering (SERS) combined with disposable chiral plasmonic shurikens and silver nanoclusters allows for effective detection and discrimination of chiral compounds with high sensitivity. Numerical simulations were used to study the effects of shuriken geometry, silver nanostructured layer parameters, and SERS excitation wavelength(s) on the observed SERS response. The designed structures were able to successfully discriminate cysteine enantiomers and probe biomolecular chirality using a standard Raman spectrometer.
NANOSCALE HORIZONS
(2023)
Article
Biochemistry & Molecular Biology
Marzuq A. Ungogo, Mustafa M. Aldfer, Manal J. Natto, Hainan Zhuang, Robyn Chisholm, Katy Walsh, MarieClaire McGee, Kayhan Ilbeigi, Jamal Ibrahim Asseri, Richard J. S. Burchmore, Guy Caljon, Serge Van Calenbergh, Harry P. De Koning
Summary: African Animal Trypanosomiasis (AAT) is a fatal livestock disease in Sub-Saharan Africa caused by Trypanosoma brucei brucei, T. vivax, and T. congolense. Limited treatment options and resistance threaten the control of this disease. In this study, the adenosine transporters of T. vivax (TvxNT3) and T. congolense (TcoAT1/NT10) were characterized and their functional expression was demonstrated. The findings suggest that nucleoside chemotherapy for AAT is viable based on the similarities in nucleoside sensitivity among different parasite species and the interactions with nucleoside transporters.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Materials Science, Biomaterials
Antonio Conde-Gonzalez, Michael Glinka, Deepanjalee Dutta, Robert Wallace, Anthony Callanan, Richard O. C. Oreffo, Mark Bradley
Summary: Regenerative medicine demands sophisticated 3D biomaterials to promote tissue formation. This study presents a novel technique for screening potential biocompatible 3D scaffolds, identifying a porous acrylate-based scaffold that promotes cell proliferation and tissue formation. The scaffold was fabricated through freeze-casting and photo-polymerization techniques and demonstrated tunable properties suitable for skeletal repair. This platform offers an innovative approach to scanning synthetic biomaterials for tissue regeneration.
BIOMATERIALS ADVANCES
(2023)
Article
Materials Science, Multidisciplinary
Martin Kartau, Anastasia Skvortsova, Victor Tabouillot, Shailendra K. Chaubey, Polina Bainova, Rahul Kumar, Vasilii Burtsev, Vaclav Svorcik, Nikolaj Gadegaard, Sang Won Im, Marie Urbanova, Oleksiy Lyutakov, Malcolm Kadodwala, Affar S. Karimullah
Summary: Chiral nanophotonic platforms enhance both the asymmetric properties and intensities of near fields, enabling optical measurements with enantiomeric discrimination at a higher detection level than conventional chirally sensitive spectroscopic methods. This study demonstrates the use of surface enhanced Raman spectroscopy (SERS) as a local probe to discriminate chiral helicoid nanoparticles on chiral metafilms. The intensity of electromagnetic hotspots generated by matched combinations of helicoid and metafilms is higher, while mismatched combinations result in cooler electromagnetic hotspots. SERS is used to detect the enantiomeric dependency of hotspot intensity, allowing the distinction between different electromagnetic environments of plasmonic diastereomers.
ADVANCED OPTICAL MATERIALS
(2023)
Article
Chemistry, Multidisciplinary
Roxanna S. Ramnarine-Sanchez, Janos M. Kanczler, Nicholas D. Evans, Richard O. C. Oreffo, Jonathan I. Dawson
Summary: This paper reports a new reaction-diffusion self-assembly system, in which proteins function as diffusive reactants to assemble stable clay-protein composite hydrogels. Using this method, scalable and highly stable 3D protein patterns can be generated at sub-cellular resolution, and can guide cell behavior to achieve precise bone tissue template formation.
ADVANCED MATERIALS
(2023)
Article
Biochemistry & Molecular Biology
Ivana Rubic, Stefan Weidt, Richard Burchmore, Alan Kovacevic, Josipa Kules, Peter David Eckersall, Marin Torti, Ines Jovic, Mislav Kovacic, Jelena Gotic, Renata Baric Rafaj, Predrag Novak, Marko Samardzija, Vladimir Mrljak
Summary: This study utilized mass spectrometry-based analysis to compare changes in plasma metabolites between dogs with idiopathic dilated cardiomyopathy (iDCM) and healthy dogs. The findings revealed altered concentrations of various metabolites involved in the development of iDCM. These results will contribute to the detection and monitoring of pathophysiological mechanisms in the development of iDCM in the future.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemical Research Methods
Duanyang Xu, Konstantinos N. Bourdakos, Anna Crisford, Peter Johnson, Ibrahim Abughazaleh, Panuwat Srisamran, Richard O. C. Oreffo, Sumeet Mahajan, David J. Richardson, Lin Xu
Summary: We present an all-fiber ized 1840-nm thulium-fiber-laser source for label-free biological imaging through nonlinear microscopy. The source consists of a dissipative-soliton mode-locked seed laser and a chirped-pulse-amplification system. It utilizes short pulses generated by the mode-locked laser and pulse compression techniques to achieve high-quality imaging of biological samples.
BIOMEDICAL OPTICS EXPRESS
(2023)
Article
Cell & Tissue Engineering
Elloise Z. Matthews, Stuart Lanham, Kate White, Maria-Eleni Kyriazi, Konstantina Alexaki, Afaf H. El-Sagheer, Tom Brown, Antonios G. Kanaras, Jonathan West, Ben D. MacArthur, Patrick S. Stumpf, Richard O. C. Oreffo
Summary: Researchers have developed a new method to isolate and enrich skeletal stem cells from human bone marrow, which have the potential to differentiate into bone, cartilage, and fat cells. This study provides valuable insights for further understanding and utilization of these stem cells.
JOURNAL OF TISSUE ENGINEERING
(2023)
Article
Materials Science, Multidisciplinary
C. Gilroy, D. J. P. Koyroytsaltis-McQuire, N. Gadegaard, A. S. Karimullah, M. Kadodwala
Summary: This study demonstrates that superchiral hot-spots are created around structural heterogeneities in all real metal structures, which possess chiral asymmetries greater than what would be expected from an idealised perfect structure. The findings suggest that surface morphology could play a role in determining the efficacy of a chiral structure for sensing applications.
MATERIALS ADVANCES
(2022)
