Article
Immunology
Qinfan Yao, Cuili Wang, Yucheng Wang, Wenyu Xiang, Yin Chen, Qin Zhou, Jianghua Chen, Hong Jiang, Dajin Chen
Summary: This study identified key differentially expressed genes (DEGs) in acute allograft rejection (AR) and found that they have the potential to be used as biomarkers for early-stage AR diagnosis. The expression of these genes can reflect the immune status of patients with AR.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Computer Science, Artificial Intelligence
Pourya Moghadam, Abbas Ahmadi
Summary: The prevalence of chronic kidney diseases has increased the demand for kidney replacement therapy. This paper proposes a novel clustering method using Red Deer Algorithm (RDA) to accurately predict kidney rejection in an imbalanced dataset. The proposed method can serve as a decision support system for clinical experts to predict kidney transplantation failure.
EXPERT SYSTEMS WITH APPLICATIONS
(2022)
Article
Urology & Nephrology
Thibaut Vaulet, Gillian Divard, Olivier Thaunat, Evelyne Lerut, Aleksandar Senev, Olivier Aubert, Elisabet Van Loon, Jasper Callemeyn, Marie-Paule Emonds, Amaryllis Van Craenenbroeck, Katrien De Vusser, Ben Sprangers, Maud Rabeyrin, Valerie Dubois, Dirk Kuypers, Maarten De Vos, Alexandre Loupy, Bart De Moor, Maarten Naesens
Summary: An international group of experts developed a consensus classification of kidney transplant rejection phenotypes known as the Banff classification. The data-driven clustering approach identified six clinically meaningful cluster phenotypes, significantly associated with graft failure, while eliminating intermediate and mixed phenotypes. This approach offers a more quantitative evaluation of rejection subtypes and severity.
JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY
(2021)
Article
Chemistry, Multidisciplinary
Penghui Cheng, Rending Wang, Shasha He, Pengpeng Yan, Hongfeng Huang, Jianghua Chen, Jia Shen, Kanyi Pu
Summary: We report the development of Artificial bioMarker Probes (AMPros) for sensitive urinalysis of Acute renal allograft rejection (ARAR) in murine models. AMPros specifically react with immune biomarkers in the kidneys and activate their fluorescence signals to detect rejection. The urinalysis using AMPros is noninvasive, sensitive, and can detect ARAR earlier than current diagnostic methods.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Multidisciplinary Sciences
Mehdi Maanaoui, Mikael Chetboun, Isabelle Top, Vincent Elsermans, Julie Kerr-Conte, Kristell Le Mapihan, Frederique Defrance, Valery Gmyr, Thomas Hubert, Myriam Labalette, Marc Hazzan, Marie-Christine Vantyghem, Francois Pattou
Summary: Islet transplantation, which requires multiple donors, is a unique form of organ transplantation. Preformed or de novo donor-specific antibodies may target one or several donor islets, and their presence is associated with different clinical outcomes.
SCIENTIFIC REPORTS
(2022)
Article
Immunology
Zijian Zhang, Yan Qin, Yicun Wang, Shuai Li, Xiaopeng Hu
Summary: This study predicted the differential expression of monocytes associated with rejection by analyzing the transcriptome data of peripheral blood mononuclear cells. A novel gene, ISG15, was found to be associated with rejection and showed a critical role in promoting T cell proliferation. The results of this study have important implications for non-invasive diagnosis and potential therapeutic targets.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Cardiac & Cardiovascular Systems
Joo Myung Lee, Ki Hong Choi, Jin-Oh Choi, Doosup Shin, Yoonjee Park, Juwon Kim, Seung Hun Lee, Darae Kim, Jeong Hoon Yang, Yang Hyun Cho, Kiick Sung, Ji Yeon Choi, Meesoon Park, Jung-Sun Kim, Taek Kyu Park, Young Bin Song, Joo-Yong Hahn, Seung-Hyuk Choi, Hyeon-Cheol Gwon, Jae K. Oh, Eun-Seok Jeon
Summary: Assessing coronary microcirculatory dysfunction with IMR early after heart transplantation is significantly associated with the risk of acute cellular rejection, making IMR a valuable clinical tool for identifying high-risk patients.
Review
Medicine, General & Internal
Farah Abuazzam, Casey Dubrawka, Tarek Abdulhadi, Gwendolyn Amurao, Louai Alrata, Dema Yaseen Alsabbagh, Omar Alomar, Tarek Alhamad
Summary: Despite advances in immunosuppressive medications, antibody-mediated rejection (AMR) remains a major cause of kidney allograft failure. Recent developments in understanding the pathophysiology of AMR and new therapeutic options have emerged. Surveillance protocols using donor-derived cell-free DNA and gene profile testing have allowed for early detection of AMR. Ongoing clinical trials offer opportunities for improving outcomes in kidney transplant recipients.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Surgery
Friedrich Alexander von Samson-Himmelstjerna, Maja Lucia Messtorff, Nassim Kakavand, Ute Eisenberger, Johannes Korth, Ulrich Lange, Benedikt Kolbrink, Leon Aldag, Tobias Schulze Dieckhoff, Thorsten Feldkamp, Ulrich Kunzendorf, Ana Harth, Kevin Schulte
Summary: Early-on post kidney transplantation, there is a high risk of graft rejection and viral infections. A low tacrolimus C/D ratio at 1 month does not predict risk, while a low ratio at 3 months is associated with subsequent rejections and impaired kidney function.
TRANSPLANT INTERNATIONAL
(2023)
Article
Biochemistry & Molecular Biology
Florian Emmerich, Stefan Zschiedrich, Christine Reichenbach-Braun, Caner Susal, Susana Minguet, Marie-Christin Pauly, Maximilian Seidl
Summary: Higher Cav-1 levels are associated with lower occurrence of acute cellular tubulointerstitial rejection episodes.
Article
Urology & Nephrology
Amaury Dujardin, Melanie Chesneau, Florian Dubois, Richard Danger, Linh Bui, Clarisse Kerleau, Pierrick Guerif, Sophie Brouard, Jacques Dantal
Summary: Patients with more than 25 years of graft survival after kidney transplantation exhibit an immunological signature associated with low rejection risk, characterized by lower total B cell percentage, higher proportion of CD24(Hi)CD38(Lo) memory B cells, fewer CD24(Lo)CD38(Lo) naive B cells, and lower proportion of PD1(Hi)CCR7(Lo) Tfh lymphocytes.
KIDNEY INTERNATIONAL
(2021)
Article
Urology & Nephrology
Hak-Jae Lee, Hyun-Wook Kwon, Jong-Kwan Baek, Chan-Hee Park, Hye-Kyung Seo, Suk-Kyung Hong
Summary: This study investigates the risk factors associated with the development of severe PCP infection with acute respiratory failure after kidney transplantation. Age, time from transplantation to PCP diagnosis, lymphopenia, and history of acute rejection within 1 year were identified as significant risk factors for severe PCP.
Article
Immunology
Hengcheng Zhang, Cecilia B. Cavazzoni, Benjamin L. Hanson, Elsa D. Bechu, Manuel A. Podesta, Jamil Azzi, Bruce R. Blazar, Anita S. Chong, Daniel Kreisel, Alessandro Alessandrini, Peter T. Sage
Summary: After allogeneic kidney transplantation, the formation of donor-specific antibodies and antibody-mediated rejection contribute to graft loss. B cells infiltrate into kidney grafts during this rejection process, however, their origins, repertoires, and functions are still unknown.
Article
Urology & Nephrology
Karim El Sakhawi, Giovanna Melica, Anne Scemla, Dominique Bertrand, Cyril Garrouste, Paolo Malvezzi, Philippe Remy, Anissa Moktefi, Alexandre Ingels, Cecile Champy, Jean-Daniel Lelievre, David Kheav, Antoine Morel, David Mokrani, Philippe Attias, Philippe Grimbert, Marie Matignon
Summary: The study found that the switch from CNI to belatacept in HIV-positive kidney allograft recipients may increase long-term kidney allograft survival rates. Results showed that the conversion treatment did not increase the risk of HIV replication after a year, but the importance of patient medication compliance and adverse events should be noted.
CLINICAL KIDNEY JOURNAL
(2021)
Article
Medicine, General & Internal
Dayana Nasr, Mahmoudreza Moein, Stephanie Niforatos, Sandy Nasr, Mulham Ombada, Farzam Khokhar, Myera Shahnawaz, Bhavya Poudyal, Maroun Bou Zerdan, Dibyendu Dutta, Reza F. Saidi, Seah H. Lim
Summary: This study investigates the impact of oral broad-spectrum antibiotics on acute graft rejection before and after kidney transplantation. The findings suggest that the use of piperacillin/tazobactam or meropenem increases the risk of acute graft rejection and shortens the development time.
JOURNAL OF CLINICAL MEDICINE
(2022)
Letter
Surgery
Ivan Zahradka, Vojtech Petr, Maria Magicova, Ondrej Viklicky
AMERICAN JOURNAL OF TRANSPLANTATION
(2022)
Article
Geriatrics & Gerontology
Tazio Maleitzke, Petra Reinke, Alison N. Agres, Sonia A. Alves, Levent Akyuz, Florian N. Fleckenstein, Anna Bichmann, Racheli Ofir, Carsten Perka, Georg N. Duda, Tobias Winkler
Summary: In this case report, we demonstrate a successful treatment of a chronic quadriceps tendon rupture (QTR) using a synergistic surgical and biological reconstructive approach. The addition of PLX-PAD cells as a local treatment option may be considered in specific cases of chronic QTRs that are not suitable for traditional surgical procedures and have a high risk of treatment failure.
JOURNAL OF CACHEXIA SARCOPENIA AND MUSCLE
(2022)
Article
Medicine, General & Internal
Ivan Zahradka, Vojtech Petr, Istvan Modos, Maria Magicova, Ladislav Dusek, Ondrej Viklicky
Summary: This retrospective observational cohort study examined the association between mRNA vaccination and SARS-CoV-2 infection rate in kidney transplant recipients (KTRs). The study found that vaccination of KTRs is associated with a lower risk for SARS-CoV-2 infection.
ANNALS OF INTERNAL MEDICINE
(2022)
Article
Microbiology
Julia Sbierski-Kind, Sophia Grenkowitz, Stephan Schlickeiser, Arvid Sandforth, Marie Friedrich, Desiree Kunkel, Rainer Glauben, Sebastian Brachs, Knut Mai, Andrea Thuermer, Aleksandar Radonic, Oliver Drechsel, Peter J. Turnbaugh, Jordan E. Bisanz, Hans-Dieter Volk, Joachim Spranger, Reiner Jumpertz von Schwartzenberg
Summary: Caloric restriction can improve metabolic health, reduce body fat accumulation, and enhance glucose tolerance, while delaying immune senescence by reducing specific cellular immune responses. This suggests that modulation of the gut microbiome could lead to the development of new therapeutic options for metabolic diseases.
Article
Medicine, Research & Experimental
Lena Peter, Desiree Jacqueline Wendering, Stephan Schlickeiser, Henrike Hoffmann, Rebecca Noster, Dimitrios Laurin Wagner, Ghazaleh Zarrinrad, Sandra Muench, Samira Picht, Sarah Schulenberg, Hanieh Moradian, Mir-Farzin Mashreghi, Oliver Klein, Manfred Gossen, Toralf Roch, Nina Babel, Petra Reinke, Hans-Dieter Volk, Leila Amini, Michael Schmueck-Henneresse
Summary: Solid organ transplant (SOT) recipients with compromised immune response are at high risk of severe COVID-19. This research proposes the adoptive transfer of tacrolimus-resistant SARS-CoV-2-specific T cells to enhance immune response in immunosuppressed patients. Promising results from laboratory analysis support the potential clinical validation of this approach in transplantation. The combinatory use of tacrolimus and tacrolimus-resistant T cells is suggested to prevent immunopathology and facilitate viral clearance in severe COVID-19 cases.
MOLECULAR THERAPY-METHODS & CLINICAL DEVELOPMENT
(2022)
Article
Medicine, Research & Experimental
Jonas Kath, Weijie Du, Alina Pruene, Tobias Braun, Bernice Thommandru, Rolf Turk, Morgan L. Sturgeon, Gavin L. Kurgan, Leila Amini, Maik Stein, Tatiana Zittel, Stefania Martini, Lennard Ostendorf, Andreas Wilhelm, Levent Akyuez, Armin Rehm, Uta E. Hoepken, Axel Pruss, Annette Kunkele, Ashley M. Jacobi, Hans-Dieter Volk, Michael Schmueck-Henneresse, Renata Stripecke, Petra Reinke, Dimitrios L. Wagner
Summary: This study optimized a virus-free genome-editing method to efficiently insert CAR into the TRAC locus of primary human T cells using CRISPR-Cas and double-stranded template DNA (dsDNA). Two pharmacological interventions were evaluated to improve cell viability and relative CAR knockin rates. The results showed that the combination of both interventions significantly increased CAR knockin rates and CAR T cell yield.
MOLECULAR THERAPY-METHODS & CLINICAL DEVELOPMENT
(2022)
Article
Cell Biology
Gabriele Brachtl, Rodolphe Poupardin, Sarah Hochmann, Anna Raninger, Karsten Jurchott, Mathias Streitz, Stephan Schlickeiser, Michaela Oeller, Martin Wolf, Katharina Schallmoser, Hans-Dieter Volk, Sven Geissler, Dirk Strunk
Summary: Donor variation is an important issue limiting cell-based therapies. The use of human platelet lysate (hPL) instead of fetal bovine serum (FBS) to propagate bone marrow stromal cells (BMSCs) can have significant effects on BMSC phenotype and function. Notably, propagation in hPL increased BMSC proliferation and gene expression trajectories, resulting in distinct surface marker signatures after just one passage. The use of FBS as a culture medium led to declining proliferative potential and limited effects on DNA methylation. Culturing BMSCs in xenogenic serum resulted in loss of function during cartilage disk formation and increased clotting risk.
Article
Cell Biology
Bella Rossbach, Krithika Hariharan, Nancy Mah, Su-Jun Oh, Hans-Dieter Volk, Petra Reinke, Andreas Kurtz
Summary: This study investigates the immunological effects of hiPSC-derived cells and finds that their immune response varies depending on the stage of cell maturation. Autologous and allogeneic T cells tolerate early renal progenitors and mature renal epithelial cells, while NK cell responses are not suppressed and can effectively change NK cell activation status. These findings have important implications for the success of cell therapies.
Article
Cell Biology
Martin Wolf, Rodolphe W. Poupardin, Patricia Ebner-Peking, Andre Cronemberger Andrade, Constantin Bloechl, Astrid Obermayer, Fausto Gueths Gomes, Balazs Vari, Nicole Maeding, Essi Eminger, Heide-Marie Binder, Anna M. Raninger, Sarah Hochmann, Gabriele Brachtl, Andreas Spittler, Thomas Heuser, Racheli Ofir, Christian G. Huber, Zami Aberman, Katharina Schallmoser, Hans-Dieter Volk, Dirk Strunk
Summary: Nanoparticles derived from therapy-grade human placental-expanded stromal cells are surrounded by a protein corona, which improves skin regeneration and angiogenesis and induces differential signaling in immune cells.
JOURNAL OF EXTRACELLULAR VESICLES
(2022)
Article
Multidisciplinary Sciences
C. Kedor, H. Freitag, L. Meyer-Arndt, K. Wittke, L. G. Hanitsch, T. Zoller, F. Steinbeis, M. Haffke, G. Rudolf, B. Heidecker, T. Bobbert, J. Spranger, H. D. Volk, C. Skurk, F. Konietschke, F. Paul, U. Behrends, J. Bellmann-Strobl, C. Scheibenbogen
Summary: Some COVID-19 patients experience long-lasting symptoms, such as fatigue and exertion intolerance, even after recovery. These symptoms are similar to those seen in non-COVID-19 patients and may be related to low-level inflammation and hypoperfusion.
NATURE COMMUNICATIONS
(2022)
Article
Allergy
Robert Sabat, Deimante Simaite, Johann Eli Gudjonsson, Theresa-Charlotte Brembach, Katrin Witte, Torben Krause, Georgios Kokolakis, Eckart Bartnik, Christos Nikolaou, Natascha Rill, Bema Coulibaly, Clement Levin, Matthias Herrmann, Gabriela Salinas, Thomas Leeuw, Hans-Dieter Volk, Kamran Ghoreschi, Kerstin Wolk
Summary: This study aimed to characterize mediators that support B/plasma cell persistence in Hidradenitis suppurativa (HS), a chronic inflammatory disease. The results showed that levels of B/plasma cells, neutrophils, CD81 T cells, and M0 and M1 macrophages were elevated in HS lesions compared to healthy skin. It was found that BAFF, produced by myeloid cells, played a key role in supporting B/plasma cell persistence and function in HS lesions.
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
(2023)
Review
Immunology
Sophie Van Linthout, Hans-Dieter Volk
Summary: This review discusses the immunological aspects connecting cancer and cardiovascular disease, and the opportunities and challenges of integrating immunotherapies and anti-inflammatory therapies in both diseases. It also discusses the use of advanced therapies like chimeric antigen receptor T cells to address the burden of cancer and cardiovascular disease. The review emphasizes the importance of using biomarkers to guide therapeutic interventions in cardiology.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Transplantation
Kamila Bednarova, Geir Mjoen, Petra Hruba, Istvan Modos, Ludek Voska, Marek Kollar, Ondrej Viklicky
Summary: This study developed a multivariable Cox model based on clinical data and a web-based nomogram to predict the recurrence and graft loss in kidney transplant recipients with IgA nephropathy (IgAN). The nomogram was externally validated and identified age, gender, retransplantation status, proteinuria, and C4d positivity as independent risk factors. The nomogram showed good predictive performance in identifying IgAN patients at risk for premature graft loss.
NEPHROLOGY DIALYSIS TRANSPLANTATION
(2023)
Article
Urology & Nephrology
Michal Schmalz, Hana Vankova, Silvie Rajnochova-Bloudickova, Petra Hruba, Martina Fialova, Jiri Gurka, Maria Magicova, Ilja Striz, Ivan Zahradka, Ondrej Viklicky
Summary: Frailty syndrome assessment may determine the most vulnerable population for an impaired humoral immune response to SARS-CoV-2 mRNA vaccination in kidney transplant recipients.
INTERNATIONAL UROLOGY AND NEPHROLOGY
(2023)
Article
Biochemistry & Molecular Biology
Christian H. Bucher, Julia C. Berkmann, Lisa-Marie Burkhardt, Carolin Paschke, Claudia Schlundt, Annemarie Lang, Angelique Wolter, Alexandra Damerau, Sven Geissler, Hans-Dieter Volk, Georg N. Duda, Katharina Schmidt-Bleek
Summary: Neutralizing immune protein could rejuvenate delayed bone fracture healing in elderly patients by reducing inflammatory responses. This study reveals the impact of immune profile on healing process and the role of interleukin-22 in delayed healing. Neutralizing IL-22 may offer a novel therapeutic strategy for accelerating healing in individuals with advanced immune experiences.
EXPERIMENTAL AND MOLECULAR MEDICINE
(2022)