Article
Cell Biology
Kimihito Nomi, Ryuhei Hayashi, Yuki Ishikawa, Yuki Kobayashi, Tomohiko Katayama, Andrew J. Quantock, Kohji Nishida
Summary: Through SEAM cells derived from human-induced pluripotent stem cells, we depict the development of the human conjunctiva. Our study reveals that EGF drives the generation of conjunctival epithelial lineage cells, while KGF is essential for the maturation of conjunctival epithelium, particularly the goblet cells.
Article
Biotechnology & Applied Microbiology
Sarah Alsobaie, Tamador Alsobaie, Amal Alshammary, Sakis Mantalaris
Summary: This study developed a method to culture lung mature type I pneumocytes from human induced pluripotent stem cells (IPSCs) in only 20 days without using feeder cells. The three-dimensional (3D) bioprocess allowed cell derivation into endoderm, and subsequently into type II alveolar epithelial cells within a very short period. This research provides an individualized model for lung engineering, therapy, and drug testing.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Cell Biology
Aline Fernanda de Souza, Fabiana Fernandes Bressan, Naira Caroline Godoy Pieri, Ramon Cesar Botigelli, Tamas Revay, Simone Kashima Haddad, Dimas Tadeu Covas, Ester Silveira Ramos, Willian Allan King, Flavio Vieira Meirelles
Summary: By reprogramming peripheral mononuclear blood cells from TS women into iPSCs and differentiating them into hPGCLCs, this study explored the feasibility of in vitro models for understanding germ cell formation mechanisms in TS.
Article
Cell & Tissue Engineering
Chanchan Song, Linli Wang, Qingyang Li, Baoyi Liao, Weihua Qiao, Qiang Li, Nianguo Dong, Liangping Li
Summary: A simple HLA gene-swapping system has been developed to generate iPSCs with specific HLA alleles, allowing for the evasion of transplant rejection by CD8+ T cells and activation of NK cells. These modified iPSCs have been successfully differentiated into functional ECs for use in cellular heart valve engineering.
STEM CELL RESEARCH & THERAPY
(2022)
Review
Genetics & Heredity
Daniel Schraivogel, Lars M. Steinmetz, Leopold Parts
Summary: Assigning functions to genes and controlling their expression are crucial in cell biology and therapeutic development. Genetic screening, especially single-cell CRISPR screening, has become an efficient and unbiased method. It has the potential to revolutionize biomedical research in the coming decades.
ANNUAL REVIEW OF GENETICS
(2023)
Article
Biochemistry & Molecular Biology
Jonathan Lozano, Alin Rai, Jarmon G. Lees, Haoyun Fang, Bethany Claridge, Shiang Y. Lim, David W. Greening
Summary: This study presents a rapid method to generate large quantities of EV-like nanovesicles from human iPSCs, which can regulate cardiac cells and have therapeutic potential. The nanovesicles carry proteins similar to the parental cells and promote angiogenesis and cardiomyocyte survival while attenuating fibroblast activation in the heart.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Multidisciplinary
Ngoc Duc Vo, Anh Thi Van Nguyen, Hoi Thi Le, Nam Hoang Nguyen, Huong Thi Thu Pham
Summary: The study developed an alternative method, the MACS-Countess system, for CD4(+) T cell counting, which combines magnetic activated cell sorting with an automated cell counting system. Compared to the FACS gold standard, the MACS-Countess system showed a mean bias of 3.5% with a narrow limit of agreement, making it a reliable and cost-effective option for laboratories in limited resource regions.
APPLIED SCIENCES-BASEL
(2021)
Article
Nanoscience & Nanotechnology
Meng-Jie Shen, Rene C. L. Olsthoorn, Ye Zeng, Thomas Bakkum, Alexander Kros, Aimee L. Boyle
Summary: The article presents a new MACS method based on coiled-coil peptide interactions, enabling efficient cell sorting of different cell lines without the need for antigen presentation, and allowing for easy detachment of magnetic particles from cells after sorting.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Multidisciplinary Sciences
Nikhil T. Awatade, Andrew T. Reid, Kristy S. Nichol, Kurtis F. Budden, Punnam Chander Veerati, Prabuddha S. Pathinayake, Christopher L. Grainge, Philip M. Hansbro, Peter A. B. Wark
Summary: This study compared the morphology and functional responses of primary bronchial epithelial cells differentiated using two commonly used culture media, PneumaCult and bronchial epithelial growth medium. The results showed distinct structural and functional differences between the two media.
SCIENTIFIC REPORTS
(2023)
Review
Biotechnology & Applied Microbiology
Miguel J. S. Ferreira, Fabrizio E. Mancini, Paul A. Humphreys, Leona Ogene, Michael Buckley, Marco A. N. Domingos, Susan J. Kimber
Summary: The use of human pluripotent stem cells in skeletal tissue engineering has shown promising results, with various methods being used for generating cartilage and bone. Emphasis is placed on generating authentic tissues rather than just differentiated cells, and new technologies are being developed to overcome challenges in this field. It is likely that cost-effective and efficacious engineered constructs using human pluripotent stem cells will be available for skeletal repair in the near future.
CRITICAL REVIEWS IN BIOTECHNOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Mattia Pasqua, Roberto Di Gesu, Cinzia Maria Chinnici, Pier Giulio Conaldi, Maria Giovanna Francipane
Summary: The review summarizes recent strategies for specification of iPSCs towards hepatobiliary lineages and their use as platforms for disease modeling and drug testing, discussing the application of tissue-engineering methods and suggesting potential pathways for improvement.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell & Tissue Engineering
Yoshinori Endo, Ken-ichiro Kamei, Kouichi Hasegawa, Keisuke Okita, Hideyuki Ito, Shiho Terada, Miho Inoue-Murayama
Summary: This study generated iPSCs from the endangered Grevy's zebra and described their molecular characteristics. The results suggest evolutionary conservation between these iPSCs and those from humans and mice, to some extent.
STEM CELLS AND DEVELOPMENT
(2022)
Article
Chemistry, Analytical
Claudia Zielke, Adriana J. Gutierrez Ramirez, Kelsey Voss, Maya S. Ryan, Azam Gholizadeh, Jeffrey C. Rathmell, Paul Abbyad
Summary: This study presents a label-free, fixation-free and passive sorting method to isolate activated T-cells shortly after activation and prior to the display of activation surface markers. The method uses a sorting platform called Sorting by Interfacial Tension (SIFT) based on pH. It successfully isolates and enriches activated T-cells on chip, and allows for the testing of T-cell activation modulators and the determination of differentiation outcomes.
Article
Biology
Faizzan S. Ahmad, Yongcheng Jin, Alexander Grassam-Rowe, Yafei Zhou, Meng Yuan, Xuehui Fan, Rui Zhou, Razik Mu-u-min, Christopher O'Shea, Ayman M. Ibrahim, Wajiha Hyder, Yasmine Aguib, Magdi Yacoub, Davor Pavlovic, Yanmin Zhang, Xiaoqiu Tan, Ming Lei, Derek A. Terrar
Summary: We demonstrate a novel approach to generate atrial-like cardiomyocytes (CMs) from human-induced pluripotent stem cells (hiPSCs) through the treatment of Gremlin 2 and retinoic acid. The generated CMs exhibited similar morphology and characteristics to adult atrial myocytes (AMs), including expression of CM proteins and striated appearance. Single-cell RNA sequencing also revealed high expression of atrial-specific transcripts in the generated CMs. This method provides a valuable tool for studying atrial cell signaling pathways and atrial fibrillation (AF).
PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Nastaran Alinezhadbalalami, Philip M. Graybill, Khan Mohammad Imran, Scott S. Verbridge, Irving C. Allen, Rafael Davalos
Summary: Expansion of tumor-activated CTLs using high-voltage pulsed electric fields is proposed as a novel method in this study, with experimental results indicating that CTL expansion is more effective under HFIRE conditions with longer pulse widths and higher voltages. This technique shows potential for clinical applications in targeting and eliminating tumors.
BIOELECTROCHEMISTRY
(2021)
Article
Cell & Tissue Engineering
Nerea Jimenez-Tellez, Carlos D. Vera, Zehra Yildirim, Julio Vicente Guevara, Tina Zhang, Joseph C. Wu
Summary: Long QT syndrome (LQTS) is a genetic cardiovascular disorder characterized by electrical conduction abnormalities, leading to arrhythmia, fainting, seizures, and an increased risk of sudden death. Researchers generated two human-induced pluripotent stem cell (iPSC) lines from LQT patients carrying a mutation in SNTA1 gene. These iPSC lines showed normal properties and pluripotency markers, making them valuable for studying the pathological mechanisms of LQTS related to the SNTA1 variant.
STEM CELL RESEARCH
(2023)
Article
Cell & Tissue Engineering
Ling Liu, Soochi Kim, Matthew T. Buckley, Jaime M. Reyes, Jengmin Kang, Lei Tian, Mingqiang Wang, Alexander Lieu, Michelle Mao, Cristina Rodriguez-Mateo, Heather D. Ishak, Mira Jeong, Joseph C. Wu, Margaret A. Goodell, Anne Brunet, Thomas A. Rando
Summary: Exercise can rejuvenate stem cells and improve tissue regeneration in aging animals. The cellular and molecular changes caused by exercise in various stem cell compartments have not been extensively studied. In this study, young and old mice were subjected to aerobic exercise and a comprehensive single-cell transcriptomic atlas of different stem cell types and their niche cells was generated. The study found that exercise ameliorated inflammatory pathways associated with old age and restored intercellular communication mediated by immune cells within these stem cell compartments. Exercise had a profound impact on the composition and transcriptomic landscape of circulating and tissue-resident immune cells.
Letter
Cardiac & Cardiovascular Systems
Mengcheng Shen, Chun Liu, Shane Rui Zhao, Amit Manhas, Laksshman Sundaram, Mohamed Ameen, Joseph C. Wu
Editorial Material
Cardiac & Cardiovascular Systems
Xuekun Wu, Francesca Vacante, Joseph C. Wu
EUROPEAN HEART JOURNAL
(2023)
Review
Physiology
Angela Zhang, Zhenqin Wu, Eric Wu, Matthew Wu, Michael P. P. Snyder, James Zou, Joseph C. C. Wu
Summary: Artificial intelligence has greatly advanced in the field of health care over the past decade, especially in utilizing physiology data to transform and improve health care. This review explores the impact of past research on the field and identifies future challenges and directions. The focus is on artificial intelligence models, their application in automating health care tasks, increasing access to care, and enhancing capabilities. The review also addresses emerging concerns regarding individual physiology data and the challenges of deploying artificial intelligence models for meaningful clinical impact.
PHYSIOLOGICAL REVIEWS
(2023)
Article
Cell & Tissue Engineering
Xiaohui Kong, Nadjet Belbachir, Wenshu Zeng, Christopher D. Yan, Sai Navada, Marco V. Perez, Joseph C. Wu
Summary: Catecholaminergic polymorphic ventricular tachycardia is a congenital arrhythmic syndrome caused by mutations on the RYR2 gene. Two patient-specific iPSC lines with RYR2 mutations were generated and evaluated for pluripotency, differentiation capability, and karyotype stability. These iPSC lines provide a valuable tool for studying the CPVT phenotype and underlying mechanisms.
STEM CELL RESEARCH
(2023)
Article
Cell & Tissue Engineering
Peter-James H. Zushin, Yang Zhou, Audrey Li, Euan A. Ashley, Matthew T. Wheeler, Joseph C. Wu
Summary: Dilated cardiomyopathies (DCM) are a leading cause of heart failure in aging populations. The chaperone protein BAG3 plays a significant role in the development of DCM and the rate at which it progresses to heart failure. In this study, two human induced pluripotent stem cell (iPSC) lines were generated from individuals with mutations in exon 3 of the BAG3 gene and their pluripotency and ability to differentiate into three primary germ layers were validated. These cell lines serve as a valuable model for studying BAG3 inactivation and insufficiency in relation to DCM.
STEM CELL RESEARCH
(2023)
Article
Cell & Tissue Engineering
Wenshu Zenga, Xiaohui Kong, Christina Alamanaa, Yu Liu, Jessica Guzman, Paul D. Pang, John W. Day, Joseph C. Wu
Summary: Spinal muscular atrophy (SMA) is a severe neurodegenerative muscular disease caused by the loss of survival of motor neuron 1 (SMN1) genes. Two iPSC lines were generated from SMA type I patients with SMN1 mutations, and their pluripotency and ability to differentiate into three germ layers were validated. These iPSC lines can be used to generate skeletal muscles to model the muscle atrophy of SMA, providing a complementary platform for in vitro drug screening.
STEM CELL RESEARCH
(2023)
Article
Cell Biology
Shasha Yin, Liu Liu, Lauren E. Ball, Yalong Wang, Mark T. Bedford, Stephen A. Duncan, Haizhen Wang, Wenjian Gan
Summary: This study identifies protein arginine methyltransferase 1 (PRMT1) as a critical regulator of the GATOR2 complex, which activates the mTORC1 pathway for tumor growth. Inhibition of the CDK5-PRMT1-WDR24 axis suppresses HCC cell proliferation and tumor growth. High PRMT1 protein expression is associated with elevated mTORC1 signaling in patients with HCC.
Article
Biology
Jui-Tung Liu, Caren Doueiry, Yu-lin Jiang, Josef Blaszkiewicz, Mary Paige Lamprecht, James A. A. Heslop, Yuri K. K. Peterson, Juliana Debrito Carten, Paula Traktman, Yang Yuan, Salman R. R. Khetani, Waleed O. O. Twal, Stephen A. A. Duncan
Summary: iPSC-derived hepatocytes were used to identify small molecule inhibitors of apoB secretion without causing steatosis. These inhibitors show effectiveness in reducing apoB secretion in cultured hepatocytes and humanized livers in mice. This research provides a potential solution for treating FH patients with hoFH mutations, with the identified small molecules having a distinct chemical structure from existing cholesterol lowering drugs.
COMMUNICATIONS BIOLOGY
(2023)
Editorial Material
Cardiac & Cardiovascular Systems
Syed Mukhtar Ahmed, Rabindra V. Shivnaraine, Joseph C. Wu
Article
Cell & Tissue Engineering
Wenqiang Liu, Wenshu Zeng, Xiaohui Kong, Min Htet, Rebecca Yu, Matthew Wheeler, John W. Day, Joseph C. Wu
Summary: Researchers successfully generated two DMD induced pluripotent stem cell lines, which not only had pluripotency but also the capability to differentiate into cells of the three germ layers in the body. These cell lines can be used to model DMD in vitro and have the potential for therapeutic development.
STEM CELL RESEARCH
(2023)
Article
Cell & Tissue Engineering
Wenjuan Zhu, Wenqiang Liu, Rebecca Yu, Melanie Manning, Anitra Waran Romfh, Joseph C. Wu
Summary: This study has successfully generated two induced pluripotent stem cell lines from two patients with Down syndrome, providing valuable resources for research and testing therapeutic strategies for this disorder.
STEM CELL RESEARCH
(2023)
Article
Cell & Tissue Engineering
Mao Zhang, Ravichandra Venkateshappa, Audrey Li, Michael B. Fowler, Melinda L. Telli, Joseph C. Wu
Summary: Researchers generated induced pluripotent stem cell (iPSC) lines from blood cells of breast cancer patients with germline ATM mutations. These patient-specific iPSC lines can differentiate into three germ layers and hold great potential for studying mechanisms and developing drug screening strategies for ATM-related cancer.
STEM CELL RESEARCH
(2023)
Letter
Cardiac & Cardiovascular Systems
Nadjet Belbachir, Yiyang Wu, Mengcheng Shen, Sophia L. Zhang, Joe Z. Zhang, Chun Liu, Bjorn C. Knollmann, Gholson J. Lyon, Ning Ma, Joseph C. Wu