Article
Parasitology
Patrick S. Sebastian, Roberto Mera y Sierra, Gisela Neira, Jaled Hadid, Fernando S. Flores, Santiago Nava
Summary: This study suggests that Rhipicephalus sanguineus s.s. may play a more important role in the transmission of Ehrlichia canis in Argentina compared to previous assumptions.
PARASITOLOGY RESEARCH
(2021)
Article
Parasitology
Maylin Gonzalez Navarrete, Adnan Hodzic, Belkis Corona-Gonzalez, Matheus Dias Cordeiro, Claudia Bezerra da Silva, Liani Coronado Baez, Dasiel Obregon, Daniel Moura de Aguiar, Amanda Noeli da Silva Campos, Isis Indaiara Goncalves Granjeiro Taques, Alejandra Wu-Chuang, Eugenio Roque Lopez, Elianne Piloto-Sardinas, Lianet Abuin-Denis, Adivaldo Henrique da Fonseca, Alejandro Cabezas-Cruz
Summary: This study characterized the genetic diversity of E. canis in Cuba based on the trp36 gene and identified a new genogroup called the 'Cuba (CUB) genogroup'. Dogs infected with the CUB genogroup showed frequent hemorrhagic lesions, suggesting that genetic diversification of E. canis in Cuba is associated with increased virulence.
PARASITES & VECTORS
(2022)
Article
Immunology
Gustavo Seron Sanches, Margarita Villar, Joana Couto, Joana Ferrolho, Isabel G. Fernandez de Mera, Marcos Rogerio Andr, Darci Moraes Barros-Battesti, Rosangela Zacarias Machado, Gervasio Henrique Bechara, Lourdes Mateos-Hernandez, Jose de la Fuente, Sandra Antunes, Ana Domingos
Summary: The study revealed differences in the proteomic profiles of tropical and temperate lineages of R. sanguineus during E. canis infection, suggesting the possibility of them belonging to different species.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Biology
Saulo Pereira Cardoso, Adenilda Cristina Honorio-Franca, Danielle Cristina Honorio Franca, Luana Paula Sales Silva, Danny Laura Gomes Fagundes-Triches, Maria Clara Bianchini Neves, Aron Carlos de Melo Cotrim, Arleana do Bom Parto Ferreira de Almeida, Eduardo Luzia Franca, Valeria Regia Franco Sousa
Summary: This study analyzed the hematological parameters, blood viscosity, and cytokines of dogs infected by Ehrlichia canis untreated and treated with doxycycline. The results showed that infected dogs had reduced leukocytes and lymphocytes, regardless of doxycycline treatment. Dogs without treatment also had decreased eosinophils and platelets. Dogs treated with doxycycline showed increased monocytes, eosinophils, and platelets.
Article
Microbiology
S. E. Acevedo-Monroy, J. M. Mendez-Aleman, I Castro-Mendoza, M. A. Mojica-Sanchez, A. Verdugo-Rodriguez
Summary: Ehrlichia canis is an increasingly important zoonotic bacterium in Mexico, but the extent of the issue in animals and public health is unknown. Local studies using serology and molecular tests have been conducted, but Ehrlichiosis is not considered endemic in the central valley of Mexico. The nested PCR method offers rapid results and high sensitivity, allowing for timely diagnosis and treatment. This method has been modified and used for the diagnosis of E. canis in dogs in Mexico, aiding in the collection of epidemiological information.
ARCHIVES OF MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Michela Pugliese, Vito Biondi, Giordana Merola, Alessandra Landi, Annamaria Passantino
Summary: This study evaluated oxidative stress parameters in canine monocytic ehrlichiosis (CME) and found that the concentration of thiol groups in plasma compounds was significantly lower in CME dogs compared to healthy dogs, and it was negatively correlated with hemoglobin concentration. These preliminary results suggest a possible role of oxidative stress in the onset of clinical signs during CME.
Article
Parasitology
AbdElkarim Laatamna, Christina Strube, Deon K. Bakkes, Sabine Schaper, Fatima Zohra Aziza, Hanan Ben Chelef, Nour El Houda Amrane, Ramlia Bedraoui, Gerhard Dobler, Lidia Chitimia-Dobler
Summary: A molecular investigation was conducted on important tick-borne pathogens in Rhipicephalus sanguineus sensu stricto collected from domestic dogs in steppe and high plateau areas of central and eastern Algeria. The study revealed a high prevalence of Hepatozoon spp., with additional detections of Babesia vogeli, Anaplasma platys, Rickettsia spp., and Ehrlichia canis. Further large scale studies are needed to better understand the epidemiology and distributions of canine tick-borne pathogens in Algeria.
Article
Zoology
Houqiang Luo, Yanfang Lan, Ping Gan, Wenjun Zhou, Meng Wang, Bing Hu, Zhuning Zhang, Yu Bai, Kun Li
Summary: The study investigated the prevalence of Rhipicephalus sanguineus and Ehrlichia canis infection in pet dogs in Wenzhou, China. The results showed that 1.29% of the serum samples were positive for E. canis and 5.50% of dogs were infested with ticks. The R. sanguineus samples in Wenzhou exhibited high homology (99.7%-99.8%) and the E. canis derived from R. sanguineus showed a similarity of 98.7%-99.7% to previously published isolates. The findings indicate the need for precautions against the potential threat posed by these ticks, especially to pet owners.
PAKISTAN JOURNAL OF ZOOLOGY
(2021)
Article
Infectious Diseases
Matthew J. Neave, Patrick Mileto, Ancy Joseph, Tristan J. Reid, Angela Scott, David T. Williams, Anthony L. Keyburn
Summary: Ehrlichia canis, a prevalent tick-borne pathogen in dogs worldwide, was detected in several dogs from Kununurra, Western Australia in May 2020. Subsequent surveillance activities revealed a significant spread of E. canis throughout northern Australia. Genomic analysis of E. canis samples from different regions in Australia suggests a potential origin from Asia or the Middle East.
TICKS AND TICK-BORNE DISEASES
(2022)
Article
Agriculture, Dairy & Animal Science
V. N. Sarawade, P. D. Pawar, P. P. Mhase, C. S. Mote, B. N. Ambore, K. Kundu, V. G. Nimbalkar, N. S. Das, L. D. Singla
Summary: The research aimed to investigate the prevalence and influence of associated risk factors on canine monocytic ehrlichiosis (CME) in dogs from Pune and Satara, Maharashtra, India. Conventional microscopy and PCR-based assays were used to detect Ehrlichia canis in blood samples from clinically ill dogs. The results showed that younger, exotic cross breed females dogs were more prone to CME compared to adults, and Indian non-descript male counterparts and winter season were more favorable for CME occurrence.
INDIAN JOURNAL OF ANIMAL RESEARCH
(2023)
Article
Veterinary Sciences
Lorenza Urbani, Alessandro Tirolo, Andrea Balboni, Roberta Troia, Francesco Dondi, Mara Battilani
Summary: This report describes the concomitant infection of CPV-2, H. canis, and E. canis in two puppy dogs from Southern Italy. Multiple diagnostic methods were used to confirm the infection, and it was found that CPV-2 belonged to the 2c variant and that H. canis and E. canis preferred to infect the same cells. It is important to carefully evaluate the movement of puppies from areas where vector-borne pathogens are endemic and to adopt appropriate preventive measures.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Veterinary Sciences
A-L Chaber, R. Easther, B. Cumming, R. Irving, Al Keyburn, C. Smart, R. O'Handley, L. Lignereux
Summary: Recent concerns have arisen in Australia due to the detection and outbreak of the exotic bacterium Ehrlichia canis. This study confirms the wide distribution range of E. canis in South Australia, spread by the tropical brown dog tick. It is important to implement effective measures to control the spread of the bacterium and minimize the impact of ehrlichiosis outbreaks.
AUSTRALIAN VETERINARY JOURNAL
(2022)
Article
Veterinary Sciences
Burcak Aslan celik, Adnan Ayan, Ali Bilgin Yilmaz, Ozgur Yasar celik, Ozlem Orunc Kilinc, Ozge Oktay Ayan
Summary: This study sequenced the 16S rRNA genetic section of Ehrlichia canis positive ticks collected from dogs in the Siirt province, Turkey, identifying 15.2% of the ticks carrying the pathogen. Sequence analysis revealed that the E. canis strains obtained were 100% identical to strains from the United States, India, and Egypt. The study identified a zoonotic agent from Rhipicephalus sanguineus ticks collected from dogs in the Siirt province.
ACTA VETERINARIA-BEOGRAD
(2021)
Article
Agriculture, Dairy & Animal Science
Boondarika Nambooppha, Amarin Rittipornlertrak, Anucha Muenthaisong, Pongpisid Koonyosying, Sahatchai Tangtrongsup, Saruda Tiwananthagorn, Yang-Tsung Chung, Nattawooti Sthitmatee
Summary: A peptide vaccine candidate, GP19(4-43), designed in this study successfully induced hyperimmune serum in rabbits, leading to a significant decrease in E. canis infection in macrophage-like cells. The treated group showed a marked reduction in the percentage of infected cells and an upregulation in IFNG expression during infections.
Article
Biotechnology & Applied Microbiology
Mingqun Lin, Qingming Xiong, Matthew Chung, Sean C. Daugherty, Sushma Nagaraj, Naomi Sengamalay, Sandra Ott, Al Godinez, Luke J. Tallon, Lisa Sadzewicz, Claire Fraser, Julie C. Dunning Hotopp, Yasuko Rikihisa
Summary: Ehrlichia sp. HF, a novel species in the genus Ehrlichia, has been stably cultured and its complete genome sequenced, showing similarities with E. chaffeensis. This species is proposed as a model for studying fatal human ehrlichiosis, providing insights into virulence factors and host inflammatory responses. The naming of this bacterium as Ehrlichia japonica sp. nov. reflects its initial isolation in Japan.
Article
Veterinary Sciences
Warley Vieira de Freitas Paula, Lucianne Cardoso Neves, Luiza Gabriella Ferreira de Paula, Maria Carolina de Azevedo Serpa, Fabricio Peixoto de Oliveira, Filipe Dantas-Torres, Sebastian Munoz-Leal, Marcelo Bahia Labruna, Felipe da Silva Krawczak
Summary: In this study, the first molecular detection of B. theileri subclinical infection in a cow in Brazil is described. The detection was confirmed using PCR, and the implications of this finding for cattle health management in the region are discussed.
VETERINARY RESEARCH COMMUNICATIONS
(2023)
Article
Infectious Diseases
Paula A. Ossa-Lopez, Lorys Y. Mancilla-Agrono, Lizeth Fernanda Banguero Micolta, Hector E. Ramirez-Chaves, Juan David Carvajal Agudelo, Sebastian Munoz-Leal, Marcelo B. Labruna, Vett Lloyd, Fredy A. Rivera-Paez
Summary: This study conducted morphological and molecular analysis on 174 larvae of Ornithodoros hasei collected from three bat species in the Orinoquia Region of Colombia. The results confirmed the species identification and showed low genetic divergence between larvae from the Arauca Department and higher genetic divergence with larvae from other American countries. This work contributes to the understanding of the distribution and potential role of O. hasei as a vector for Borrelia.
TICKS AND TICK-BORNE DISEASES
(2023)
Editorial Material
Public, Environmental & Occupational Health
Sergio E. Bermudez, Alvaro A. Faccini-Martinez, Felipe S. Krawczak
TRAVEL MEDICINE AND INFECTIOUS DISEASE
(2023)
Article
Agriculture, Dairy & Animal Science
Lucianne Cardoso Neves, Warley Vieira de Freitas Paula, Luiza Gabriella Ferreira de Paula, Bianca Barbara Fonseca da Silva, Sarah Alves Dias, Brenda Gomes Pereira, Bruno Sergio Alves Silva, Anaia da Paixao Seva, Filipe Dantas-Torres, Marcelo B. Labruna, Felipe da Silva Krawczak
Summary: Human cases of Brazilian spotted fever (BSF) have been recently reported in Goias state, Brazil, but the agent responsible for these cases remains unknown. In this study, antibodies against Rickettsia spp. were detected in dogs, horses, and capybaras, and rickettsial DNA was found in ticks collected from these animals and the environment. The DNA belonged to Rickettsia bellii, a species not belonging to the spotted fever group (SFG). Seroreactivity to SFG and R. bellii antigens was observed in animals, indicating the circulation of SFG rickettsiae in the region.
Article
Agriculture, Dairy & Animal Science
Shafi Ullah, Abdulaziz Alouffi, Mashal M. Almutairi, Nabila Islam, Gauhar Rehman, Zia Ul Islam, Haroon Ahmed, Itabajara da Silva Vaz, Marcelo B. Labruna, Tetsuya Tanaka, Abid Ali
Summary: Ticks are blood-feeding ectoparasites that can transmit life-threatening pathogens to humans and animals. This study detected Rickettsia conorii for the first time in the Hyalomma kumari ticks collected from goats and sheep in Pakistan. This finding is important for the surveillance and prevention of zoonotic diseases.
Article
Microbiology
Felipe S. Krawczak, Lina C. Binder, Fabio Gregori, Thiago F. Martins, Gracielle T. Padua, Jonas Sponchiado, Geruza L. Melo, Gina Polo, Marcelo B. Labruna
Summary: This study investigated the rickettsial infection in ticks, domestic dogs, and small mammals in a natural reserve in the Pampa biome in southern Brazil. The main vector, Amblyomma tigrinum, was not found to carry Rickettsia parkeri, but instead carried the non-pathogenic agent 'Candidatus Rickettsia andeanae'. The study area was determined not to be endemic for R. parkeri rickettsiosis, and a negative correlation was observed between the infection rates of R. parkeri and 'Candidatus R. andeanae' in A. tigrinum populations.
Article
Infectious Diseases
Luis E. Romero, Lina C. Binder, Arlei Marcili, Marcelo B. Labruna
Summary: This study investigated tick infestation in domestic dogs in El Salvador and identified five tick species. It also detected three spotted fever group Rickettsia agents, suggesting the occurrence of spotted fever cases caused by R. parkeri strain Atlantic rainforest in El Salvador.
TICKS AND TICK-BORNE DISEASES
(2023)
Article
Infectious Diseases
Vanessa Lima de Souza, Francisco Borges Costa, Thiago Fernandes Martins, Patricia Rosa de Oliveira, Jonatas Lima, David Pedroza Guimara, Ednaira Alencar dos Santosb, Nicole Oliveira de Moura-Martiniano, Tayra Pereira Sato, Ana Beatriz Pais Borsoi, Karla Bitencourth, Gilberto Salles Gazeta, Edson Guilherme, Francisco Glauco de Arujo Santos
Summary: The study aimed to investigate tick species and rickettsial agents on wild birds captured in the Western Amazon. Ticks were collected from 607 birds and identified using morphological and molecular analyses. The study found new host-parasite associations with different tick species and detected rickettsial DNA fragments, including R. parkeri and R. tamurae-like. The findings highlight the need for further exploration of the public health implications and new host-parasite interactions in this underexplored region.
TICKS AND TICK-BORNE DISEASES
(2023)
Article
Infectious Diseases
Joao F. Soares, Marcelo B. Labruna, Derek B. de Amorim, Vinicius Baggio-Souza, Renata Fagundes-Moreira, Aline Girotto-Soares, Barbara Weck, Pablo H. Nunes, Thiago F. Martins
Summary: Adult hard ticks were collected from great horned owls in southern Brazil in 2020. The analysis of their external morphology revealed a new tick species, named Amblyomma monteiroae n. sp. Genetic sequencing showed that this new species is closely related to Amblyomma parvitarsum Neumann, 1901. This study increases the Amblyomma tick species in Brazil to a total of 34.
TICKS AND TICK-BORNE DISEASES
(2023)
Article
Infectious Diseases
Hermes R. Luz, Marcelo B. Labruna, Richard C. Pacheco, Sergio L. Gianizella, Pablo H. Nunes, Matias P. J. Szabo, Monize Gerardi, Rodrigo H. F. Teixeira, Silvio C. da Silva, Louise B. Kmetiuk, Isabella P. Pesenato, Arlei Marcili, Joao L. H. Faccini, Thiago F. Martins
Summary: Abnormalities in external morphology were observed in 31 individual adult ticks of 15 different species collected from various locations in Brazil. These abnormalities included local anomalies such as malformations of body parts and general anomalies such as duplication of the opisthosoma. The findings expand the list of anomalous tick species in the Neotropics.
TICKS AND TICK-BORNE DISEASES
(2023)
Article
Microbiology
Shehla Shehla, Farman Ullah, Abdulaziz Alouffi, Mashal M. Almutairi, Zaibullah Khan, Tetsuya Tanaka, Marcelo B. Labruna, Kun-Hsien Tsai, Abid Ali
Summary: This study investigated the tick species infesting livestock in Pakistan and the presence of Rickettsia spp. The results showed the distribution of Rickettsia species in ticks and identified certain species that can cause zoonotic diseases.
Review
Microbiology
Jose de la Fuente, Agustin Estrada-Pena, Marta Rafael, Consuelo Almazan, Sergio Bermudez, Abdelbaset E. Abdelbaset, Paul D. Kasaija, Fredrick Kabi, Foluke Adedayo Akande, Dorcas Oluwakemi Ajagbe, Timothy Bamgbose, Srikant Ghosh, Azhahianambi Palavesam, Penny H. Hamid, Charlotte L. Oskam, Siobhon L. Egan, Amanda Duarte-Barbosa, Olcay Hekimoglu, Matias P. J. Szabo, Marcelo B. Labruna, Ananta Dahal
Summary: In this comprehensive review study, the challenge posed by ticks and tick-borne diseases (TBDs) with growing incidence affecting global human and animal health was addressed. Data and perspectives from different countries and regions were collected to update the current situation with ticks and TBDs and highlight the existing information bias and gaps perceived by society. The study emphasizes the importance of multidisciplinary and international collaborations in advancing surveillance, communication, and proposed future directions to address these challenges.
Article
Agronomy
Gracielle Teles Padua, Luiza Gabriella Ferreira de Paula, Ana Carolina Borsanelli, Marcelo Bahia Labruna, Felipe da Silva Krawczak
Summary: Dermacentor nitens Neumann is a common tick species in horses in Brazil, mainly infesting the ears, nasal diverticulum, perineal and perianal regions. Infestations can cause severe damage, anemia, and can also transmit Babesia caballi. This study reports an unusual parasitic site of D. nitens on a formerly enucleated eye orbit in a female cross breed horse.