Review
Biochemistry & Molecular Biology
Hsuan Peng, Kazuhiro Shindo, Renee R. Donahue, Ahmed Abdel-Latif
Summary: Stem cell-based cardiac therapies present challenges in cell delivery, engraftment, and differentiation, but still lead to sustained cardiac benefits. Current research focuses on exploring the mechanisms of stem cell-mediated cardiac immunomodulation, with efforts to improve current strategies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell & Tissue Engineering
Marta Rojas-Torres, Ismael Sanchez-Gomar, Antonio Rosal-Vela, Lucia Beltran-Camacho, Sara Eslava-Alcon, Jose Angel Alonso-Pineiro, Javier Martin-Ramirez, Rafael Moreno-Luna, Ma Carmen Duran-Ruiz
Summary: Before translating ECFCs into clinical treatment, it is necessary to perform biodistribution assays to ensure biosafety and determine the distribution of cells. This study optimized a cell-tracking assay using DiR labeling and qPCR analysis, and found that intramuscularly administered ECFCs mainly remained in the ischemic tissues, while intravenously injected cells were found in multiple organs.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Medicine, Research & Experimental
Shi Hua Tan, Sze Jie Loo, Yu Gao, Zhong Hao Tao, Li Ping Su, Chen Xu Wang, Sophia L. Zhang, Yong Hui Mu, Ying Hua Cui, Desiree Abdurrachim, Wei Hsin Wang, Janise Lalic, Kheng Choon Lim, Jun Bu, Ru San Tan, Teck Hock Lee, Jianyi Zhang, Lei Ye
Summary: In a porcine model of myocardial infarction, co-treatment with Tb4-microspheres and hiPSC-CMs improved the engraftment and reparative potency of the transplanted hiPSC-CMs, induced vasculogenesis and proliferation of cardiomyocytes and endothelial cells, improved left ventricular systolic function, and reduced infarct size. HiPSC-CM implantation did not increase the incidence of ventricular arrhythmias and did not induce tumorigenesis in the immunosuppressed pigs.
Review
Cell Biology
Wei-Zhan Zhuang, Yi-Heng Lin, Long-Jyun Su, Meng-Shiue Wu, Han-Yin Jeng, Huan-Cheng Chang, Yen-Hua Huang, Thai-Yen Ling
Summary: Mesenchymal stem/stromal cells (MSCs) are a promising resource for cell-based therapy due to their high immunomodulation ability and tropism towards inflamed and injured tissues. However, the lack of standardized methods to evaluate the safety, efficacy, and biodistribution of MSCs has been a major hindrance to their clinical application.
JOURNAL OF BIOMEDICAL SCIENCE
(2021)
Review
Cell Biology
Juan Huang, Qi Feng, Li Wang, Bingying Zhou
Summary: Cardiac diseases are the leading cause of global mortality, but limited progress has been made in developing therapeutic options. The use of animal models has limitations due to species-specific differences, leading to inaccurate drug testing results. Human pluripotent stem cell (hPSC) technology offers promising alternatives for heart disease modeling, cell therapy, and drug discovery, with potential for revolutionizing these fields with further optimization required.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Polymer Science
Cecilie Hoeeg, Alireza Dolatshahi-Pirouz, Bjarke Follin
Summary: Cell therapy has the potential to regenerate cardiac tissue and treat various cardiac diseases. Injectable hydrogels have been investigated for their potential to improve cell graft retention and enhance the effects of cardiac cell therapy.
Article
Pharmacology & Pharmacy
Marieke T. Roefs, Wolf Heusermann, Maike A. D. Brans, Christian Snijders Blok, Zhiyong Lei, Pieter Vader, Joost P. G. Sluijter
Summary: This study investigated the fate of cardiac progenitor cell-derived extracellular vesicles (CPC-EVs) in vivo and found that after intramyocardial injection, CPC-EVs did not immediately flush out of the heart but migrated in the myocardial tissue, while after intravenous injection, most CPC-EVs accumulated in the liver. The study also demonstrated that decorating EVs with targeting peptides enhanced EV delivery to cardiac endothelial cells.
FRONTIERS IN PHARMACOLOGY
(2022)
Review
Cell & Tissue Engineering
Ramana Vaka, Darryl R. Davis
Summary: This article discusses the current state of cellular therapies for cardiac repair and regeneration, outlining the progression of new ideas from preclinical literature to ongoing clinical trials. Recent data supporting the mechanics and mechanisms of myogenic and paracrine therapies are evaluated in the context of long-term cardiac engraftment, informing on promising new approaches for future avenues in the field.
Article
Biochemistry & Molecular Biology
Adel Hinsenkamp, Kiara Kun, Fatime Gajnut, Aliz Majer, Zsombor Lacza, Istvan Hornyak
Summary: Fibrin membranes are widely used in regenerative medicine due to their biocompatibility, biodegradability, high growth factor content, and cell attachment support. Cryoprecipitate isolation can increase fibrinogen and platelet concentration in plasma, affecting membrane thickness and growth factor content. However, membrane thickness does not significantly influence cell attachment, as demonstrated by live-dead staining and XTT measurements.
Article
Multidisciplinary Sciences
Chae Moon Hong, Prakash Gangadaran, Ji Min Oh, Ramya Lakshmi Rajendran, Arunnehru Gopal, Liya Zhu, Byeong-Cheol Ahn
Summary: This study developed a nuclear imaging method for tracking EVs derived from cell lines, showing that radioiodine-labeled EVs had good biodistribution. The radioiodine labeling did not significantly affect the morphology and properties of EVs.
SCIENTIFIC REPORTS
(2021)
Article
Cell Biology
Bimal Gurung, Gary Tse, Wendy Keung, Ronald A. Li, Wing Tak Wong
Summary: The study shows that hypokalaemia has pro-arrhythmic effects on hvCAS, leading to changes in conduction velocity, repolarization, refractoriness, and calcium handling. This bioengineered platform provides a useful tool for investigating electrophysiological substrates and conducting arrhythmia screening.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Engineering, Biomedical
Ferdous B. Finklea, Yuan Tian, Petra Kerscher, Wen J. Seeto, Morgan E. Ellis, Elizabeth A. Lipke
Summary: The study demonstrates successful production of functional cardiac tissue microspheres through direct differentiation of hydrogel encapsulated hiPSCs, with high reproducibility and cell viability. These microspheres support efficient cardiac differentiation and have potential applications in biomanufacturing, drug screening, and regenerative therapies.
Article
Cardiac & Cardiovascular Systems
Zhongkai Wang, Pan Li, Bili Zhang, Jingjuan Huang, Shaoping Chen, Zhuhong Cai, Yingyi Qin, Jihai Fan, Wendong Tang, Yongwen Qin, Ruogu Li, Xianxian Zhao
Summary: The combination of SyncAV with BiV leads to a more significant reduction in QRS duration and improves LV remodeling in HF patients undergoing CRT therapy, compared to conventional BiV pacing. During follow-up, no deaths related to HF deterioration or severe procedure complications occurred.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2021)
Article
Multidisciplinary Sciences
Lu Meng, Fengmin Yang, Yan Pang, Zhenping Cao, Feng Wu, Deyue Yan, Jinyao Liu
Summary: This study demonstrates the use of cationic polymers to selectively cap bacteriophages, enabling the entry of phages into intestinal epithelial cells and escaping from endosomes. This nanocapping technique significantly reduces the translocation of pathogens and shows potential for developing next-generation antimicrobials.
Review
Biochemistry & Molecular Biology
Sara Ghodrat, Seyed Javad Hoseini, Shiva Asadpour, Simin Nazarnezhad, Fariba Alizadeh Eghtedar, Saeid Kargozar
Summary: Stem cells play a crucial role in promoting angiogenesis and improving cardiac healing and remodeling processes. They can enhance angiogenesis by differentiating into endothelial lineages and secreting paracrine factors to drive neo-vessel formation. Conducting further research is necessary to identify the molecular mechanisms behind stem cells' proangiogenic potential, including signaling pathways and regulatory molecules like microRNAs.
