Article
Nanoscience & Nanotechnology
Ziwei Liu, Chen Cai, Wengang Wu, Xinxia Cai, Zhi-mei Qi
Summary: Nanostructured films possess unique electrical, optical, and plasmonic characteristics due to their nano-size effect. In this study, a hyperspectral dark-field microscope (HSDFM) was employed to characterize nanostructured films with high resolution, revealing different optical properties of various samples and showcasing the applicability of HSDFM for thin-film device optimization.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Chemistry, Analytical
Austin F. Dunn, Megan A. Catterton, Drake D. Dixon, Rebecca R. Pompano
Summary: The study developed an optimized method to detect dynamic and spatially resolved glucose uptake in living tissue using a fluorescent glucose analogue and tissue slice culture. This new approach provides a user-friendly platform for quantifying dynamic metabolic activities in complex tissue microenvironments, with potential applications in various research fields.
ANALYTICA CHIMICA ACTA
(2021)
Article
Chemistry, Analytical
Yang Zeng, Rui Cao, Jie Zhu, Wei Zhao, Dan Sun, Ce Zhang
Summary: A methodology using dark field microscopy to capture live cell behavior is proposed, with minimal phototoxicity compared to traditional bright-field and fluorescence microscopy. The approach is suitable for long-term live cell imaging with super-high temporal resolution, as demonstrated by continuous exposure experiments on fibroblasts and hepatoma cells.
ANALYTICAL METHODS
(2022)
Article
Chemistry, Analytical
Xi Chen, Zhongwei Jiang, Ling Liang, Yuan Fang Li, Cheng Zhi Huang, Peng Fei Gao
Summary: By utilizing the Au NBPs@ZIF-8 nanoprobe, the fluctuation of ATP level in living cells can be monitored with high sensitivity, selectivity, and biocompatibility. This method demonstrates a good linear relationship, low detection limit, and excellent selectivity, allowing for effective observation of intracellular ATP changes.
ANALYTICAL CHEMISTRY
(2022)
Article
Biochemical Research Methods
Thomas Mangeat, Simon Labouesse, Marc Allain, Awoke Negash, Emmanuel Martin, Aude Guenole, Renaud Poincloux, Claire Estibal, Anais Bouissou, Sylvain Cantaloube, Elodie Vega, Tong Li, Christian Rouviere, Sophie Allart, Debora Keller, Valentin Debarnot, Xia Bo Wang, Gregoire Michaux, Mathieu Pinot, Roland Le Borgne, Sylvie Tournier, Magali Suzanne, Jerome Idier, Anne Sentenac
Summary: The study introduces a super-resolution microscopy method called random illumination microscopy (RIM), which is suitable for live-cell imaging and easy to implement. RIM's simplicity and extended biological applicability can help biology laboratories to more easily utilize super-resolution microscopy.
CELL REPORTS METHODS
(2021)
Article
Plant Sciences
Hai-Yan Jiang, Hui-Yu Gao, Jie Li, Tian -Yu Zhou, Shu-Ting Wang, Jian-Bo Yang, Rui-Rui Hao, Fei Pang, Feng Wei, Zhi-Gang Liu, Lian Kuang, Shuang-Cheng Ma, Jiu-Ming He, Hong-Tao Jin
Summary: The study comprehensively identified the hepatotoxicity mechanisms of PM-D through an integrated spatially resolved metabolomics and network toxicology strategy, unveiling key targets, pathways, and metabolic changes associated with PM-D-induced liver injury. The findings provide a theoretical basis for understanding the toxicity of PM and its safe clinical use.
JOURNAL OF ETHNOPHARMACOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Stefano Mariani, Antonino Amedeo La Mattina, Alessandro Paghi, Lucanos Strambini, Giuseppe Barillaro
Summary: A fluoride-assisted method for controlled in-situ synthesis of metal nanoparticles on PDMS is reported, allowing modulation of NP size and coverage over time and space. This method enables the preparation of linear and step gradients of NPs on PDMS surface, showing potential for flexible plasmonics/photonics applications.
ADVANCED FUNCTIONAL MATERIALS
(2021)
Article
Multidisciplinary Sciences
Jacopo Valsecchi, Youngju Kim, Seung Wook Lee, Kotaro Saito, Christian Grunzweig, Markus Strobl
Summary: In the past decade, neutron dark-field contrast imaging has evolved from a qualitative tool to a quantitative spatial resolved small-angle scattering instrument, allowing for quantitative assessment of microscopic structures from the nanometer to micrometer range. The introduction of polarized neutron grating interferometric imaging has enabled the development of a polarized and polarization analyzed dark-field contrast method for studying magnetic microstructures with spatially resolved small-angle scattering. This method allows for the separation and measurement of spin-flip and non-spin-flip small-angle scattering, as well as the potential for distinguishing nuclear and different magnetic contributions in the analyzed small-angle scattering.
SCIENTIFIC REPORTS
(2021)
Article
Biology
Yifeng Shi, Shiyi Peng, Zhongyu Huang, Zhe Feng, Wen Liu, Jun Qian, Weidong Zhou
Summary: This study demonstrates that gold nanorods can significantly improve dark-field imaging of colorectal cancer cells in the NIR-II spectrum, and can be used for specific labeling of colorectal cancer cell nuclei.
Article
Electrochemistry
F. F. Oehler, A. Graule, S. Kuecher, T. Roth, A. Adam, J. Li, E. Ronge, R. Moertel, A. Jossen
Summary: This study presents a novel method for measuring local potentials in lithium-ion batteries and addresses the stability issue of multiple reference electrodes. Experimental results demonstrate the high reproducibility and applicability of this method.
JOURNAL OF THE ELECTROCHEMICAL SOCIETY
(2023)
Article
Computer Science, Cybernetics
Shu-Hsien Liao, Retno Widowati, Pimchanok Puttong
Summary: This research examines the behaviors of various users of FB Live in Thailand through an empirical survey research and shows how the FB Live study and social media/network analytics results can obtain streamers/users knowledge patterns and rules investigating different users' profiles and behaviors to develop social commerce and business models in Thailand.
ENTERTAINMENT COMPUTING
(2022)
Article
Optics
Chanjun Sun, Ben Aernouts, Wouter Saeys
Summary: This research investigates the mismatches between measurements and simulations by studying the influences from light source and the incident and detection angle on diffuse reflectance profiles. After reducing the mismatches caused by these factors, metamodels linking optical properties with diffuse reflectance profiles were built based on measured and simulated data, both achieving high prediction performance with R-2 values above 0.96, allowing for the creation of simulation-based metamodel applicable to different measurement configurations.
Article
Biochemistry & Molecular Biology
Ilona D. Nordhorn, Doerthe Dietrich, Christine Verlemann, Antje Vennemann, Robin Schmid, Matthias Elinkmann, Joshua Fuchs, Michael Sperling, Martin Wiemann, Uwe Karst
Summary: In a dual approach, laser ablation-inductively coupled plasma-mass spectrometry was used to investigate the translocation of polyvinylpyrrolidone-coated gold nanoparticles from the lungs to the spleen in rats. Spatially resolved imaging analysis and single particle analysis were deployed to determine the distribution pattern and species of translocated gold, showing the translocation of intact gold nanoparticles in a non-agglomerated state.