Article
Multidisciplinary Sciences
Yongchan Lee, Pattama Wiriyasermkul, Pornparn Kongpracha, Satomi Moriyama, Deryck J. Mills, Werner Kuhlbrandt, Shushi Nagamori
Summary: The study reveals the molecular basis of system b(0,+) biogenesis and type I cystinuria, by demonstrating the role of Ca2+ in stabilizing the interface between regulatory subunits and facilitating higher-order assembly. These findings provide valuable insights for understanding cystinuria and developing new therapeutic strategies, and also establish a connection between transporter oligomeric assembly and protein-trafficking diseases.
NATURE COMMUNICATIONS
(2022)
Article
Microbiology
Sunyia Hussain, Janine H. Peterson, Harris D. Bernstein
Summary: The assembly of trimeric porins involves a stepwise process that requires the presence of the Bam complex and LPS. The interaction with LPS is not only essential for trimerization but also for the productive insertion of individual subunits into the lipid bilayer.
Article
Chemistry, Multidisciplinary
Xueqin Yang, Lijiao Yang, Donglei Yang, Min Li, Pengfei Wang
Summary: Cell membrane proteins play a crucial role in regulating intracellular signal transductions and cell behaviors. By utilizing membrane-anchored DNA hybridization chain reaction, DNA self-assembly can be induced on the live cell surface, leading to the clustering of membrane proteins and modulation of cell behaviors.
Article
Biochemistry & Molecular Biology
Yilmaz Yucehan Yazici, Serkan Belkaya, Emel Timucin
Summary: IL18 is a pro-inflammatory cytokine that regulates immune responses. The activity of IL18 is controlled by IL18BP. The structure and dynamics of the IL18-IL18BP heterodimers are affected by a short surface epitope between amino acids 68 and 81. MD simulations showed that the helical form of this epitope stabilizes the complex and contributes to IL18-IL18BP stability.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2023)
Article
Chemistry, Multidisciplinary
Jue Yin, Mo Xie, Junke Wang, Meirong Cui, Dan Zhu, Shao Su, Chunhai Fan, Jie Chao, Qian Li, Lianhui Wang
Summary: In this work, the authors report a simple and versatile one-pot strategy to fabricate DNA architectures with the assistance of spherical gold nanoparticles modified with thiolated oligonucleotide strands. The desired architectures are formed via the stochastic co-assembly of different modules.
Article
Chemistry, Physical
Sandra C. C. Nunes, A. A. C. C. Pais
Summary: Introduces the first step in gene delivery applications, which is the interaction with negatively charged cell membranes. Discusses the complexity and factors influencing the interaction between DNA and cell membranes, and presents the use of Monte Carlo simulations to determine the optimal conditions for DNA-polyplex adsorption.
JOURNAL OF MOLECULAR LIQUIDS
(2022)
Article
Multidisciplinary Sciences
Matthew Charman, Nicholas Grams, Namrata Kumar, Edwin Halko, Joseph M. Dybas, Amber Abbott, Krystal K. Lum, Daniel Blumenthal, Elene Tsopurashvili, Matthew D. Weitzman
Summary: Biomolecular condensates formed by phase separation can compartmentalize and regulate cellular processes. Evidence suggests that membraneless subcellular compartments in virus-infected cells also form by phase separation. Here we demonstrate that phase separation of the human adenovirus 52-kDa protein plays a critical role in the coordinated assembly of infectious progeny particles. Our findings identify essential requirements for coordinated assembly of progeny particles and demonstrate that phase separation of a viral protein is critical for production of infectious progeny during adenovirus infection.
Article
Biochemistry & Molecular Biology
Nicolo Alvisi, Fabiola A. Gutierrez-Mejia, Meike Lokker, Yu-Ting Lin, Arthur M. de Jong, Floris van Delft, Renko de Vries
Summary: Control over biomolecule placement and activity on solid surfaces is crucial in bionanotechnology. Recombinant protein engineering can be used to design protein-based diblock polymers that self-assemble on silica surfaces and form stable polypeptide brushes for biofunctionalization. The stability of these brushes is partly due to peptide-peptide interactions at the silica surface, as evidenced by high unbinding rates of silica-binding peptides and fibrillar self-assembly induced by silica binding.
Article
Chemistry, Multidisciplinary
Jiahui Wu, Nina Svensen, Wenjiao Song, Hyaeyeong Kim, Sulei Zhang, Xing Li, Samie R. Jaffrey
Summary: DNA and RNA can self-assemble into various structures, and there are methods available to achieve RNA self-assembly in cells. The use of dimeric aptamers allows the creation of RNA and RNA-protein assemblies in cells. These studies provide an approach for inducing RNA self-assembly, demonstrate that proteins can be recruited to RNA assemblies to genetically encode intracellular RNA-protein assemblies.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)
Article
Chemistry, Multidisciplinary
Zheng Yuan, Dapeng Zhang, Fangzhi Yu, Yangde Ma, Yan Liu, Xiangjun Li, Hailin Wang
Summary: This study demonstrates a new supernuclease's capability for highly efficient cutting of DNA segments, leading to the development of a method that allows precise sequencing of single protected-DNA fragment molecules, and the direct measurement of RecA filament length. This innovative assay shows promise as an analytical tool for studying various protein-DNA interactions involved in DNA replication, transcription, recombination, repair, and gene editing.
Article
Multidisciplinary Sciences
Yiming Yu, Shige H. Yoshimura
Summary: CIP4 drives asymmetric closing in clathrin-mediated endocytosis through LLPS-driven self-assembly and stereospecific interaction, which generates an actin-rich microenvironment near the pit. This study provides mechanistic insights into how disordered and structured domain collaboration promotes spatio-temporal actin polymerization near the plasma membrane.
NATURE COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Xundong Wei, Jianhua Zhang, Jian Cui, Wei Xu, Xuyu Zhou, Jie Ma
Summary: The Split-Cre system allows for precise gene manipulation in cell subpopulations by improving recombinase activity through GFP-mediated dimerization and SpyTag/SpyCatcher conjugation. This dual-split system enhances gene expression control and provides advanced analysis of genes and cell functions in complex tissues like the immune system.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Review
Microbiology
Matthew Thomas Doyle, Harris D. Bernstein
Summary: The Omp85 protein superfamily is a group of proteins found in the outer membrane of gram-negative bacteria and bacterial-origin eukaryotic organelles. These proteins are involved in both membrane insertion and translocation of proteins across the outer membrane. Recent studies have uncovered new insights into the functions of these proteins, particularly the well-studied member BamA, which is essential for bacterial barrel assembly and has potential implications for antibiotic development.
ANNUAL REVIEW OF MICROBIOLOGY
(2022)
Article
Cell Biology
Samuel S. Wallis, Leandro N. Ventimiglia, Evita Otigbah, Elvira Infante, Miguel Angel Cuesta-Geijo, Gururaj Rao Kidiyoor, M. Alejandra Carbajal, Roland A. Fleck, Marco Foiani, Sergi Garcia-Manyes, Juan Martin-Serrano, Monica Agromayor
Summary: Research reveals the crucial role of the ESCRT-associated protein BROX in NE repair, showing that its interaction with Nesprin-2G helps alleviate cytoskeletal forces and promote efficient repair of NE ruptures. This mechanism contributes to maintaining nuclear-cytoplasmic compartmentalization and genomic stability.
DEVELOPMENTAL CELL
(2021)
Article
Multidisciplinary Sciences
Oliver G. Hayes, Benjamin E. Partridge, Chad A. Mirkin
Summary: This study demonstrates the deliberate control of hierarchical assembly of protein-DNA materials by exploiting the chemical anisotropy of proteins and the programmability of DNA ligands. The introduction of orthogonal DNA interactions with disparate strengths onto specific geometric regions of a model protein leads to directional assembly and increased multi valency through DNA sequence design. The judicious DNA design not only directs assembly along a specific pathway but also influences distinct structural outcomes from a single pathway.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Chemistry, Multidisciplinary
Vladimir N. Podust, Sibu Balan, Bee-Cheng Sim, Michael P. Coyle, Ulrich Ernst, Robert T. Peters, Volker Schellenberger
JOURNAL OF CONTROLLED RELEASE
(2016)
Article
Chemistry, Multidisciplinary
Stacy L. Capehart, Michael P. Coyle, Jeff E. Glasgow, Matthew B. Francis
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2013)
Article
Chemistry, Multidisciplinary
Theobald Lohmueller, Sara Triffo, Geoff P. O'Donoghue, Qian Xu, Michael P. Coyle, Jay T. Groves
