Journal
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
Volume 135, Issue 28, Pages 10254-10257Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ja404276g
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Funding
- National Creative Research initiatives of MEST/NRF of Korea (Center for Single-Molecule Systems Biology)
- National Research Foundation of Korea
- Korean Government [2009-0087691]
- U.S. National Institutes of Health [GM051290-18]
- Korea Institute of Science and Technology
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Current single-molecule techniques do not permit the real-time observation of multiple proteins interacting closely with each other. We here report an approach enabling us to determine the single-molecule fluorescence resonance energy transfer (FRET) kinetics of multiple protein-protein interactions occurring far below the diffraction limit. We observe a strongly cooperative formation of multimeric soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) complexes, which suggests that formation of the first SNARE complex triggers a cascade of SNARE complex formation.
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