Article
Dermatology
Yu Jeong Cho, So-Hyun Nam, Eugene Oh, Michael P. Luciano, Choonghee Lee, Il Hyung Shin, Martin J. Schnermann, Jaepyeong Cha, Kwan Woo Kim
Summary: Bile duct injury during laparoscopic cholecystectomy is a common complication, and identifying bile duct during surgeries remains a challenge. This study demonstrated successful visualization of the biliary tract using a novel fluorescent dye in a swine model, suggesting its potential as a valuable tool for hepato-biliary surgeries.
LASERS IN SURGERY AND MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Harrison C. Daly, Siddharth S. Matikonda, Helena C. Steffens, Bastian Ruehle, Ute Resch-Genger, Joseph Ivanic, Martin J. Schnermann
Summary: The study demonstrates that simple modifications on the xanthene dye scaffold can extend into the SWIR range for deep tissue imaging.
PHOTOCHEMISTRY AND PHOTOBIOLOGY
(2022)
Article
Biochemical Research Methods
Ek Raj Thapaliya, Syed Muhammad Usama, Nimit L. Patel, Yang Feng, Joseph D. Kalen, Brad St Croix, Martin J. Schnermann
Summary: This study systematically investigates the impact of various functional groups on the properties of antibody-fluorophore conjugates, and suggests that zwitterionic substituents possess an optimal combination of high tumor signal, tumor-to-background ratio, and low liver-to-background ratio.
BIOCONJUGATE CHEMISTRY
(2022)
Article
Biochemical Research Methods
Venu G. Bandi, Michael P. Luciano, Mara Saccomano, Nimit L. Patel, Thomas S. Bischof, Jakob G. P. Lingg, Peter T. Tsrunchev, Meredith N. Nix, Bastian Ruehle, Chelsea Sanders, Lisa Riffle, Christina M. Robinson, Simone Difilippantonio, Joseph D. Kalen, Ute Resch-Genger, Joseph Ivanic, Oliver T. Bruns, Martin J. Schnermann
Summary: Through rational design, red-shifted, bioconjugatable heptamethine cyanine dyes were developed for multiplexed in vivo imaging in the shortwave-infrared/near-infrared-II region. The use of wavelengths between 1,000 and 2,000 nm enables high-resolution in vivo imaging at depths not possible with conventional optical wavelengths. Recent progress has shown a lack of bioconjugatable probes for imaging in the shortwave-infrared or near-infrared (NIR)-II range.
Editorial Material
Biochemistry & Molecular Biology
Martin J. Schnermann, Youngjae You
PHOTOCHEMISTRY AND PHOTOBIOLOGY
(2022)
Article
Oncology
Syed Muhammad Usama, Sierra C. Marker, Servando Hernandez Vargas, Solmaz AghaAmiri, Sukhen C. Ghosh, Naruhiko Ikoma, Hop S. Tran Cao, Martin J. Schnermann, Ali Azhdarinia
Summary: This review provides an overview of the design considerations and fluorescent building blocks of targeted dual-modal imaging agents, as well as potential future directions in this field. Targeted dual-modal imaging agents have the potential to improve surgical workflow, including preoperative patient selection and surgical planning, as well as fluorescence-guided surgery.
Review
Biochemical Research Methods
Siddharth S. Matikonda, Ryan McLaughlin, Pradeep Shrestha, Carol Lipshultz, Martin J. Schnermann
Summary: Antibody-drug conjugates (ADCs) are a rapidly growing class of cancer therapeutics that aim to overcome the limitations of conventional cytotoxic agents. This review describes different ADCs combinations and technologies, with a focus on the anti-HER-2 antibody trastuzumab and its variants.
BIOCONJUGATE CHEMISTRY
(2022)
Article
Biophysics
Syed Muhammad Usama, Donald R. Caldwell, Pradeep Shrestha, Michael P. Luciano, Nimit L. Patel, Joseph D. Kalen, Joseph Ivanic, Martin J. Schnermann
Summary: This study details the synthesis and evaluation of a class of norcyanine compounds that enable pH responsive fluorescence imaging in the long wavelength range by installing a hydrophilic substituted quaternary amine, showing promising photophysical properties.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Chemistry, Multidisciplinary
Hiroshi Fukushima, Siddharth S. Matikonda, Syed Muhammad Usama, Aki Furusawa, Takuya Kato, Lenka Stackova, Petr Klan, Hisataka Kobayashi, Martin J. Schnermann
Summary: Photoconvertible tracking strategies, such as PACT, are used to evaluate the migration of immune cells into tumor-draining lymphatics. PACT utilizes a recently discovered cyanine photoconversion reaction and near-infrared wavelengths, eliminating the need for genetic engineering. This approach provides a straightforward and quantitative method for studying cell migration dynamics.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)
Article
Chemistry, Organic
Quan T. Khong, Donghao Li, Brice A. P. Wilson, Kalina Ranguelova, Masoumeh Dalilian, Emily A. Smith, Antony Wamiru, Ekaterina I. Goncharova, Tanja Grkovic, Donna Voeller, Stanley Lipkowitz, Martin J. Schnermann, Barry R. O'Keefe, Lin Du
Summary: A new dimeric alkaloid, Plakoramine A, was discovered from a marine sponge. It showed potent inhibition of ubiquitin ligase activities. The study also revealed a previously undescribed route to form this compound through photochemical conversion.
Article
Chemistry, Multidisciplinary
Dong-Hao Li, Rananjaya S. Gamage, Allen G. Oliver, Nimit L. Patel, Syed Muhammad Usama, Joseph D. Kalen, Martin J. Schnermann, Bradley D. Smith
Summary: This study reports a synthetic method for producing novel doubly strapped zwitterionic heptamethine cyanine dyes with enhanced stability. These dyes, including a structural analogue of ZW800-1, can be conjugated to proteins, including monoclonal antibodies, without causing fluorophore degradation or dye stacking. The fluorescent antibody conjugates show excellent tumor-targeting specificity in a xenograft mouse tumor model. The enhanced stability provided by the doubly strapped molecular design enables new classes of in vivo NIR fluorescence imaging experiments with possible translation to humans.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Dermatology
Michele S. Saruwatari, Kochai Jawed, Khalid M. Ali, Bo Ning, Sailee Naik, So-Hyun Nam, Martin J. Schnermann, Anthony Sandler, Richard J. Cha
Summary: This study compared the use of BL-760 and ICG for detecting bile leakage during surgery. The results showed that BL-760 could rapidly detect bile leakage with a high fluorescence- to- liver parenchyma ratio, making it a potential tool for identifying bile leakage and duct injury.
LASERS IN SURGERY AND MEDICINE
(2023)
Review
Biochemistry & Molecular Biology
Martin J. Schnermann, Luke D. Lavis
Summary: The field of organic chemistry originated in the 19th century with scientists identifying and expanding synthetic dye molecules for textiles. In the 20th century, dye chemistry continued to develop with a focus on creating photographic sensitizers and laser dyes. In the 21st century, the advancement of biological imaging techniques has become a driving force for dye chemistry, especially in the development of rhodamines and cyanines, which are widely used in biological imaging.
CURRENT OPINION IN CHEMICAL BIOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Anna C. Love, Donald R. Caldwell, Bethany Kolbaba-Kartchner, Katherine M. Townsend, Lila P. Halbers, Zi Yao, Caroline K. Brennan, Joseph Ivanic, Tanya Hadjian, Jeremy H. Mills, Martin J. Schnermann, Jennifer A. Prescher
Summary: To enable multicomponent bioluminescence imaging in vivo, we developed a new class of near-infrared emitting coumarin luciferin analogues (CouLuc-3s) that can penetrate tissues. These scaffolds were easily synthesized from commercially available dyes. We also identified mutant luciferases that can specifically interact with the CouLuc-3 analogues. The brightest probes allowed for sensitive in vivo imaging. The CouLuc-3 scaffolds are orthogonal to popular bioluminescent reporters, making them suitable for multicomponent imaging applications.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Computer Science, Artificial Intelligence
William J. Godinez, Eric J. Ma, Alexander T. Chao, Luying Pei, Peter Skewes-Cox, Stephen M. Canham, Jeremy L. Jenkins, Joseph M. Young, Eric J. Martin, W. Armand Guiguemde
Summary: Recent advances in generative modelling, specifically the use of deep neural networks, have enabled the design of novel compounds. This paper introduces a generative modelling approach called JAEGER, which is built on top of the JT-VAE neural network model. The aim of JAEGER is to find new chemical matter with desired bioactivity, and it has been successfully applied to design compounds for inhibiting malaria. The results show that JAEGER is a viable approach for finding novel active compounds for drug discovery.
NATURE MACHINE INTELLIGENCE
(2022)