Article
Chemistry, Multidisciplinary
Yusuke Takezawa, Shiori Sakakibara, Mitsuhiko Shionoya
Summary: DNA three-way junction (3WJ) structures serve as essential building blocks for constructing DNA nanoarchitectures. By inducing structure through the addition or removal of Ni-II ions, as well as performing Ni-II-mediated self-sorting using bpy-modified strands and their unmodified counterparts, potential applications for metal-responsive DNA materials can be further developed.
CHEMISTRY-A EUROPEAN JOURNAL
(2021)
Review
Biochemistry & Molecular Biology
Shunsuke Takahashi, Masahiko Oshige, Shinji Katsura
Summary: DNA replication, repair, and recombination in cells regulate genetic information transfer. Single-molecule imaging provides new insights into the dynamic behaviors of individual biomolecules, helping analyze the interaction between DNA and proteins.
Article
Biochemistry & Molecular Biology
Javier Jarillo, Borja Ibarra, Francisco Javier Cao-Garcia
Summary: The analysis and modeling techniques of in vitro single-molecule DNA replication events play a crucial role in understanding the process, with a focus on the real-time kinetics of DNA polymerase and DNA helicase. Proper analysis of single-molecule data is essential for obtaining a detailed picture of the system's dynamics, including kinetics rates, equilibrium constants, and conformational changes. These techniques can also be adapted to study the operation of other proteins involved in nucleic acids metabolism.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2021)
Article
Biochemical Research Methods
Yunwei Zhao, Hui Zhang, Ying Wang, Yanfang Zhao, Yaowei Li, Lei Han, Lihua Lu
Summary: A simple fluorescence detection platform based on DNA three-way junctions has been developed for the assay of acetamiprid, tripling the fluorescence signal without the need for any other amplification. This platform utilizes G-quadruplex assembly to achieve sensitive detection of acetamiprid and demonstrates high potential for applications in food control and environmental monitoring.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Wei Zhang, Zhibin Zhao, Min Tan, Adila Adijiang, Shurong Zhong, Xiaona Xu, Tianran Zhao, Emusani Ramya, Lu Sun, Xueyan Zhao, Zhiqiang Fan, Dong Xiang
Summary: The binding orientation of molecules with respect to the electrode is crucial for the performance of molecular devices. However, modulating the binding orientation of single molecules in situ remains challenging. In this study, we demonstrate that the conductance of pyridine-anchored molecular junctions can be controlled by the applied voltage using a STM-BJ technique. Flicker noise measurements and theoretical calculations support the notion that the orientation of nitrogen-gold bonds in the molecular junctions can be manipulated by the mechanical stretching force and electric fields.
Article
Multidisciplinary Sciences
Masahiko Ajiro, Tomonari Awaya, Young Jin Kim, Kei Iida, Masatsugu Denawa, Nobuo Tanaka, Ryo Kurosawa, Shingo Matsushima, Saiko Shibata, Tetsunori Sakamoto, Rolenz Studer, Adrian R. Krainer, Masatoshi Hagiwara
Summary: Research has shown that the small molecule splice modulator RECTAS can enhance the inclusion of exon 20 of the IKBKAP gene causing familial dysautonomia by promoting the binding of the splicing enhancer factor SRSF6. RECTAS interacts with CDC-like kinases (CLKs) and enhances SRSF6 phosphorylation to achieve its effects. By regulating cellular CLK activity, RECTAS can bidirectionally manipulate exon 20 splicing, which has been validated in multiple FD disease models.
NATURE COMMUNICATIONS
(2021)
Article
Chemistry, Analytical
Yapiao Li, Fangzhi Yu, Shaokun Chen, Ning Zhang, Hailin Wang, Qiang Zhao
Summary: This study presents an immunoassay mediated by a triply functional DNA probe for detecting small-molecule targets. The method allows for the separation of antibody-probe complexes using capillary electrophoresis (CE) and achieves simple and highly sensitive detection. The approach was demonstrated to simultaneously detect two model small-molecule targets, showing potential for wide applications.
ANALYTICAL CHEMISTRY
(2021)
Article
Medicine, Research & Experimental
Maria Troisi, Mitchell Klein, Andrew C. Smith, Gaston Moorhead, Yonatan Kebede, Raymond Huang, Elliott Parker, Hector Herrada, Elizabeth Wade, Samara Smith, Payson Broome, Jonah Halsell, Louis Estevez, Anthony J. Bell
Summary: This study aims to analyze the structure and protein recognition features of branched DNA four-way junctions, with a focus on the therapeutic potential of these molecules. The results show that inserting hairpins into the junctions can increase stability and nuclease resistance, while maintaining similar protein binding affinity as the control.
EXPERIMENTAL BIOLOGY AND MEDICINE
(2021)
Article
Materials Science, Multidisciplinary
Yingnan Zhang, Changhao Fang, Wildemar S. P. Carvalho, Yongfeng Gao, Michael J. Serpe
Summary: The light- and temperature-responsive poly(N-isopropylacrylamide-co-nitrobenzyl methacrylate) microgels demonstrated efficient release of fluorescein through UV irradiation-induced cleavage and heating-induced deswelling, showing potential applications in triggered cargo release systems.
ACS APPLIED POLYMER MATERIALS
(2021)
Article
Nanoscience & Nanotechnology
Yeongkwon Kang, Jung Won Yoon, Seok Woo Lee, Hyungju Ahn, Siwon Yun, Jongchul Lim, Jihoon Lee, Dong Wook Chang, Hyosung Choi, Bong-Gi Kim
Summary: To investigate the effect of miscibility between conjugated polymers (CPs) and Y6 on the morphology of bulk-heterojunction (BHJ) structures, three different CPs with similar chemical structures but varying miscibility with Y6 were studied. The interface morphology and interlocked dimensions were compared quantitatively using a square-wave model after selectively removing Y6 from the CP/Y6 blend films. It was found that as CP-Y6 miscibility increases, a higher intermixed interface is formed, while a decrease in miscibility leads to a decrease in the dimensions formed by phase separation and an increase in width.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Chemistry, Multidisciplinary
Chun-yan Qu, Li-sha Zhou, Li-hong Shu, Qian Huang
Summary: A stable tetrahedron-assisted probe was developed for the detection of multiple miRNAs, allowing simultaneous detection of miRNA-21 and miRNA-144. The method showed good agreement with qRT-PCR in clinical samples, suggesting significant potentials in early cancer diagnosis and treatment.
Article
Biochemistry & Molecular Biology
Pinghua Ling, Shan Cheng, Linyu Wang, Xinyu Sun, Xianping Gao, Feng Gao
Summary: In this study, the differential binding ability of aminoglycosides to DNA structures was investigated using electrochemical method and principal component analysis (PCA). It was found that the structure and sequence of DNA, as well as the size and structure of small molecules, can affect the binding ability between aminoglycosides and DNA. This research presents a new approach for classifying DNA structure and designing targeted drugs.
BIOELECTROCHEMISTRY
(2022)
Article
Multidisciplinary Sciences
Heysol C. Bermudez-Cabrera, Sannie Culbertson, Sammy Barkal, Benjamin Holmes, Max W. Shen, Sophia Zhang, David K. Gifford, Richard Sherwood
Summary: The mutational outcomes of CRISPR-Cas9 editing are predictable and targeted, and the ATM inhibitor KU-60019 has been shown to increase 1-bp insertions at the targeted locus. This small molecule has the potential to improve the efficiency of correction for pathogenic 1-bp deletion variants, adding another dimension to precise genome editing with Cas9 nucleases.
NATURE COMMUNICATIONS
(2021)
Article
Chemistry, Multidisciplinary
Hua Chai, Peng Miao
Summary: A novel electrochemical strategy combining strand displacement amplification and DNA nanostructures was developed for ultrasensitive detection of ctDNA, achieving a limit of detection as low as 48 amol/L. This proposed electrochemical biosensor shows great potential for clinical applications.
CHINESE CHEMICAL LETTERS
(2021)
Article
Chemistry, Multidisciplinary
Qigang Zhong, Alexander Ihle, Sebastian Ahles, Hermann A. Wegner, Andre Schirmeisen, Daniel Ebeling
Summary: Constructing low-dimensional covalent assemblies with tailored size and connectivity is challenging but important in molecular electronics for tuning the properties of quantum materials. A versatile approach has been developed for building such structures block by block on a surface through highly selective tip-induced intermolecular reactions.
Article
Chemistry, Multidisciplinary
Andreas Schachtschneider, Martin Wessig, Martin Spitzbarth, Adrian Donner, Christian Fischer, Malte Drescher, Sebastian Polarz
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2015)
Article
Chemistry, Multidisciplinary
Filip Wojciechowski, Andreas Gross, Isabelle T. Holder, Laura Knoerr, Malte Drescher, Joerg S. Hartig
CHEMICAL COMMUNICATIONS
(2015)
Article
Biochemical Research Methods
Martin Spitzbarth, Malte Drescher
JOURNAL OF MAGNETIC RESONANCE
(2015)
Article
Chemistry, Multidisciplinary
Stefan Scheerer, Michael Linseis, Evelyn Wuttke, Sabrina Weickert, Malte Drescher, Oliver Troeppner, Ivana Ivanovic-Burmazovic, Andreas Irmler, Fabian Pauly, Rainer F. Winter
CHEMISTRY-A EUROPEAN JOURNAL
(2016)
Review
Chemistry, Physical
Christian Hintze, Ulrich E. Steiner, Malte Drescher
Article
Multidisciplinary Sciences
Martin Spitzbarth, Tobias Lemke, Malte Drescher
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2016)
Article
Chemistry, Organic
Holger Busskamp, Sascha Keller, Marta Robotta, Malte Drescher, Andreas Marx
BEILSTEIN JOURNAL OF ORGANIC CHEMISTRY
(2014)
Article
Biochemistry & Molecular Biology
Marta Robotta, Hanne R. Gerding, Antonia Vogel, Karin Hauser, Stefan Schildknecht, Christiaan Karreman, Marcel Leist, Vinod Subramaniam, Malte Drescher
Article
Chemistry, Multidisciplinary
Stephan M. Hacker, Christian Hintze, Andreas Marx, Malte Drescher
CHEMICAL COMMUNICATIONS
(2014)
Correction
Chemistry, Multidisciplinary
Moritz J. Schmidt, Julia Borbas, Malte Drescher, Daniel Summerer
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2014)
Article
Chemistry, Multidisciplinary
Moritz J. Schmidt, Julia Borbas, Malte Drescher, Daniel Summerer
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2014)
Article
Chemistry, Physical
Silvia Domingo Koehler, Sebastian Hoefel, Malte Drescher
Article
Chemistry, Multidisciplinary
Charlotte Rehm, Isabelle T. Holder, Andreas Gross, Filip Wojciechowski, Maximilian Urban, Malte Sinn, Malte Drescher, Joerg S. Hartig
Review
Biochemistry & Molecular Biology
Theresa Braun, Malte Drescher, Daniel Summerer
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2019)
Article
Biology
Jessica Droeden, Malte Drescher
Summary: In this study, rapid scan (RS) electron paramagnetic resonance (EPR) spectroscopy was used to investigate the intermolecular interactions of intrinsically disordered proteins (IDPs). The results showed that RS EPR spectroscopy can provide insights into the aggregation kinetics and highlight the key features of Parkinson's disease. Compared to traditional EPR methods, RS EPR spectroscopy offers 5-fold higher sensitivity and significantly reduces data acquisition time.
