Review
Chemistry, Applied
Junqiao Wang, Jing Zhao, Shaoping Nie, Mingyong Xie, Shaoping Li
Summary: This review summarizes the recent research progress on MALDI mass spectrometry (MS) detection in food carbohydrates, including the improvement of ionization efficiency, the application in structure characterization of polysaccharides, and the quantitative study and mass spectrometry imaging.
Review
Polymer Science
Paola Rizzarelli, Marco Rapisarda
Summary: In-depth structural and compositional investigation of biodegradable polymeric materials is crucial for their successful applications. Advanced mass spectrometry techniques, such as tandem mass spectrometry (MS/MS), have been increasingly applied in biodegradable polymer studies for detailed structure characterization and degradation monitoring. This review discusses the application of matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) and electrospray ionization mass spectrometry (ESI-MS) MS/MS in biodegradable polymers.
Article
Chemistry, Applied
Yunhe Hong, Nicholas Birse, Brian Quinn, Yicong Li, Wenyang Jia, Saskia van Ruth, Christopher T. Elliott
Summary: In this study, the difference between wild and farmed salmon production was successfully characterized using matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-ToF MS) combined with chemometric analysis. The established method involved multivariate analysis techniques, such as principal component analysis (PCA), partial least squares-discriminant analysis (PLS-DA), and orthogonal partial least squares-discriminant analysis (OPLS-DA), to identify distinctive features in wild and farmed salmon products. The proposed method achieved 100% classification accuracy in discriminating farmed and wild salmon, as well as distinguishing different species of wild salmon. The results suggest that this method could be used as a reference for detecting salmon fraud related to production type and expand the application of MALDI-ToF technology in food authenticity testing.
Article
Biochemistry & Molecular Biology
Marco Roverso, Roberta Seraglia, Raghav Dogra, Denis Badocco, Silvia Pettenuzzo, Luca Cappellin, Paolo Pastore, Sara Bogialli
Summary: This study evaluated the use of single-walled carbon nanohorns (SWCNH) as a specific surface for Surface-Assisted Laser Desorption Ionization (SALDI) analysis. The optimized method provided rapid analysis of amino acids and lipids, and accurately determined the composition of fatty acids in olive oil samples without any sample treatment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Oncology
Rita Casadonte, Mark Kriegsmann, Katharina Kriegsmann, Isabella Hauk, Rolf R. Meliss, Cornelia S. L. Mueller, Joerg Kriegsmann
Summary: This study utilized imaging mass spectrometry to compare the proteomic profiles of cutaneous melanomas and melanocytic nevi in a large cohort, identifying a signature that can distinguish malignant melanoma from benign nevus with an overall accuracy of over 98%. The prediction model was validated in an independent set with an accuracy of 93% in classifying melanoma from nevi. Statistical analysis of the imaging mass spectrometry data revealed significant variations in mass-to-charge ratio peaks between the two tissue types. This study represents the largest imaging mass spectrometry investigation of cutaneous melanoma and nevi to date, demonstrating the potential for IMS to discriminate between melanocytic nevi and melanoma.
Article
Biotechnology & Applied Microbiology
Hangjun Huang, Haiqiang Liu, Weiwei Ma, Liang Qin, Lulu Chen, Hua Guo, Hualei Xu, Jinrong Li, Chenyu Yang, Hao Hu, Ran Wu, Difan Chen, Jinchao Feng, Yijun Zhou, Junli Wang, Xiaodong Wang
Summary: A novel metabolomics analysis technique called MALDI-MSI-PTMA was developed to detect and image metabolites from plant tissues. This technique overcomes the limitation of intact plant tissues by homogenizing plant tissues, preparing PTMA moulds, and spraying matrix on PTMA sections. The technique has advantages such as no need of sample separation and enrichment, high-throughput metabolite detection and imaging, high-stability mass spectrometry data acquisition and imaging reconstruction, and high reproducibility of data.
PLANT BIOTECHNOLOGY JOURNAL
(2023)
Article
Chemistry, Analytical
Huihui Liu, Ting Xie, Jiyun Wang, Xiao Wang, Jing Han, Zhihua Huang, Lixia Jiang, Zongxiu Nie
Summary: HIE is a major cause of neurological disability and fetal or neonatal asphyxia death. The study found significant changes in metabolites involved in multiple metabolic pathways during the HIE period, including the accumulation of neutral TGs in the ischemic region.
Article
Oncology
Rita Casadonte, Joerg Kriegsmann, Mark Kriegsmann, Katharina Kriegsmann, Roberta Torcasio, Maria Eugenia Gallo Cantafio, Giuseppe Viglietto, Nicola Amodio
Summary: Appropriate sample preparation is critical for the analysis of cell cultures with mass spectrometry. In this study, two different sample preparation procedures for proteomic analysis of multiple myeloma (MM) cells were evaluated using imaging mass spectrometry (IMS). The results showed that cytospin preparation resulted in better signal-to-noise ratios compared to formalin fixation and paraffin embedding. IMS technology holds the potential to stratify different cell lines and identify differentially expressed proteins.
Article
Microbiology
Marceau Levasseur, Teo Hebra, Nicolas Elie, Vincent Guerineau, David Touboul, Veronique Eparvier
Summary: In this study, we proposed a workflow using a standardized protein and lipid extraction protocol and reliable spectral comparison algorithm in MetGem software for classification and identification of environmental microorganisms. Our method was validated and shown to be capable of classifying bacteria at the order and genus level, and fungi at the order level.
Article
Chemistry, Analytical
Valentina V. Ilyushenkova, Marina E. Zimens, Nikolay Yu Polovkov, Artyom P. Topolyan, Roman S. Borisov, Vladimir G. Zaikin
Summary: This study presents an efficient method for the highly sensitive determination of dipeptides in biological liquids. The method involves derivatization of peptides followed by mass spectrometry, which allows for the detection of low quantities of dipeptides. The approach was successfully applied to model dipeptides in artificial urine and blood serum, and several dipeptides were identified in real blood serum samples. The use of collision induced dissociation generated characteristic fragmentation mass spectra for rapid identification and quantification. The derivatized dipeptides showed higher sensitivity compared to non-modified dipeptides. Overall, this derivatization approach is valuable for the identification of specific dipeptides in artificial media and dietary supplements.
Review
Chemistry, Analytical
Judy Gopal, Manikandan Muthu
Summary: Mushrooms have transitioned from a luxury food to a widely accepted nutrient-rich food. Mushroom polysaccharides have become icons of mushroom bioactivity, and the analytical techniques for their analysis have been consolidated and summarized.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Article
Biochemical Research Methods
Brett R. Hamilton, Weili Chan, Karen L. Cheney, Robert K. P. Sullivan, Matthias Floetenmeyer, Mary J. Garson, Roger Wepf
Summary: In this paper, we investigate the presence of a toxin in a nudibranch and demonstrate improved resolution and sensitivity for subcellular molecular imaging. The findings suggest the potential for subcellular 3D volume reconstruction.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2022)
Review
Biochemistry & Molecular Biology
Madhuri Jayathirtha, Emmalyn J. Dupree, Zaen Manzoor, Brianna Larose, Zach Sechrist, Anca-Narcisa Neagu, Brindusa Alina Petre, Costel C. Darie
Summary: The human genome consists of around 30,000 genes, making humans slightly more complex than worms or flies. However, the complexity of humans mainly comes from the diverse proteins encoded by these genes, as well as the production of multiple proteins through alternative splicing and tissue-dependent expression. Post-translational modifications in proteins significantly increase the number of gene products or protein isoforms.
CURRENT PROTEIN & PEPTIDE SCIENCE
(2021)
Review
Biochemistry & Molecular Biology
Weifu Ren, Qi Bian, Yan Cai
Summary: Kidney disease is a global health concern that requires upgraded diagnostic tools to diagnose and prevent disease progression at an early stage. N-glycosylation, as an important protein modification, plays a crucial role in renal structure and function. Research on the role of N-glycosylation in kidney disease provides insights into its pathophysiology and paves the way for clinical applications.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2022)
Review
Chemistry, Analytical
Quan He, Cuirong Sun, Jian Liu, Yuanjiang Pan
Summary: This article discusses the role of matrix-assisted laser desorption/ionization mass spectrometry imaging in cancer drug development and therapy, emphasizing its importance in revealing drug distribution and metabolism.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2021)
