Journal
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
Volume 131, Issue 37, Pages 13305-13314Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ja902662e
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Funding
- Engineering and Physical Sciences Research Council [GR/T09224]
- University of Nottingham
- University of Warwick
- BBSRC [BB/E009042/1] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/E009042/1] Funding Source: researchfish
- Engineering and Physical Sciences Research Council [GR/T09224/01] Funding Source: researchfish
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Flow linear dichroism (LD) spectroscopy provides information on the orientation of molecules in solution and hence on the relative orientation of parts of molecules. Long molecules such as fibrous proteins can be aligned in Couette flow cells and characterized using LD. We have measured using Couette flow and calculated from first principles the LD of proteins representing prototypical secondary structure classes: a self-assembling fiber,and tropomyosin (all-alpha-helical), FtsZ (an alpha beta protein), an amyloid fibril (beta-sheet), and Collagen [poly(proline)II helices]. the combination of calculation and experiment allows elucidation of the protein orientation in the Couette flow and the orientation of chromophores within the protein fibers.
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