Article
Neurosciences
Ishak Suat Ovey, Ahmet Ozsimsek, Halil Aziz Velioglu, Ozlem Altay, Adil Mardinoglu, Burak Yulug
Summary: This study aimed to investigate the effects of EGb 761 on a controlled model of pentylenetetrazole-induced generalized epilepsy. The results showed that EGb 761 reduced Ca release, restored apoptosis, ROS changes, mitochondrial depolarization and caspase levels, suggesting its prophylactic and therapeutic effects. It is the first study to investigate the combined effects of EGb 761 and pentylenetetrazole on TRP channels in a relevant model of human epileptic diseases.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2023)
Article
Pharmacology & Pharmacy
Ranwa A. Elrayess, Mahmoud E. Mohallal, Yomn M. Mobarak, Hala M. Ebaid, Sarah Haywood-Small, Keith Miller, Peter N. Strong, Mohamed A. Abdel-Rahman
Summary: According to recent studies, Smp24 and Smp43 peptides found in the venom of the Egyptian scorpion have shown cytotoxicity towards both tumor and non-tumor cells. These peptides activate a programmed pyroptotic mechanism to inhibit cell growth.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Pharmacology & Pharmacy
King-Chuen Wu, Kar-Lok Wong, Lian-Ru Shiao, Cing-Yu Chen, Paul Chan, Yuk-Man Leung
Summary: This study revealed two novel actions of lidocaine in neuronal cells: depletion of Ca2+ store in an IP3R- and RYR-independent manner, and suppression of store-operated Ca2+ influx.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2021)
Article
Biochemical Research Methods
Kohei Yamaguchi, Kenta Yokoi, Masakazu Umezawa, Koji Tsuchiya, Yasuyuki Yamada, Shin Aoki
Summary: In this study, triptycene-peptide hybrids were designed and synthesized, and it was found that certain compounds with conjugated structures induced a new type of cell death called paraptosis in leukemia T-lymphocytes. Mechanistic studies revealed that these compounds induced a series of cellular processes leading to paraptosis.
BIOCONJUGATE CHEMISTRY
(2022)
Article
Chemistry, Medicinal
Ye Wu, Dong Lu, Yixin Jiang, Jinmei Jin, Sanhong Liu, Lili Chen, Hong Zhang, Yudong Zhou, Hongzhuan Chen, Dale G. Nagle, Xin Luan, Weidong Zhang
Summary: The study demonstrates that the novel stapled peptides, Ano-3/3s, based on peptide stapling chemistry, show superior protease-resistance and selective cytotoxicity against tumor cells, inducing tumor ablation through direct oncolytic effect and immunogenic cell death. In vivo experiments reveal a more remarkable response to Ano-3/3s in melanoma, suggesting the potential of these peptides as effective agents for oncolytic therapy.
JOURNAL OF MEDICINAL CHEMISTRY
(2021)
Article
Multidisciplinary Sciences
Takatoshi Uchida, Shota Shimizu, Reiko Yamagishi, Suzumi M. Tokuoka, Yoshihiro Kita, Megumi Honjo, Makoto Aihara
Summary: The study found that Piezo1 is abundantly expressed in the trabecular meshwork and can regulate trabecular meshwork cell contraction by modulating Ca2+ influx and release of arachidonic acid and PGE(2). This suggests that Piezo1 could act as a regulator to control intraocular pressure.
SCIENTIFIC REPORTS
(2021)
Article
Multidisciplinary Sciences
Hyungyu Min, Wooseob Kim, Sehoon Hong, Sungkyu Lee, Jinguk Jeong, Seyun Kim, Rho H. Seong
Summary: This study reveals that inositol polyphosphate multikinase (IPMK) is a crucial regulator of Treg cell differentiation downstream of TCR signaling, and deficiency of IPMK leads to abnormal T cell activation and impaired Treg cell differentiation and function.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Review
Biotechnology & Applied Microbiology
Julien Giribaldi, Jennifer J. Smith, Christina I. Schroeder
Summary: Animal venoms contain bioactive peptides that modulate cellular excitability and show potential in anticancer therapy. Strategies such as bioconjugation and nanotechnologies could enhance the selectivity and potency of venom peptides against cancer cells.
BIOTECHNOLOGY ADVANCES
(2021)
Article
Biochemistry & Molecular Biology
Daniel Janitschke, Anna Andrea Lauer, Cornel Manuel Bachmann, Jakob Winkler, Lea Victoria Griebsch, Sabrina Melanie Pilz, Elena Leoni Theiss, Heike Sabine Grimm, Tobias Hartmann, Marcus Otto Walter Grimm
Summary: Alzheimer's disease is characterized by increased plaque formation and tangle accumulation in the brain, along with extensive lipid alterations. Methylxanthines, a type of alkaloid commonly consumed through diet, have been found to interfere with the molecular mechanisms leading to Alzheimer's disease. Our study shows that methylxanthines not only affect triglycerides and cholesterol in the liver and serum, but also induce changes in other lipid classes in neuroblastoma cells. These changes include both beneficial and adverse effects related to Alzheimer's disease. Therefore, we suggest combining methylxanthines with a diet that alters lipid metabolism to counteract the adverse effects.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biophysics
Josette Zaklit, Gale L. Craviso, Normand Leblanc, P. Thomas Vernier, Esin B. Sozer
Summary: The cellular effects of nanosecond-pulsed electric field exposures can be attenuated by bipolar pulse cancellation using equal duration and amplitude positive and negative phases, with response restoration achieved through appropriate interphase intervals. The modulation of Ca2+ responses in neural-type cells with ultrashort pulses demonstrates the potential for using nanosecond bipolar pulse technologies for neuromodulation applications.
BIOPHYSICAL JOURNAL
(2021)
Article
Biochemistry & Molecular Biology
Kongdong Li, Chuanzhi Guo, Jiacheng Ruan, Bo Ning, Chris Kong-Chu Wong, Haifeng Shi, Jie Gu
Summary: Cadmium (Cd2+) exposure-induced cytotoxicity and renal injury can be protected against by activating the calcium-sensing receptor (CaSR) to restore the intracellular Ca2+ homeostasis through the sarco/endoplasmic reticulum Ca2+-ATPase (SERCA) channel. SERCA2 degradation caused by Cd2+ can be inhibited by the proteasome inhibitor MG132, indicating the involvement of the proteasomal protein degradation pathway in maintaining SERCA2 stability. Targeting SERCA2 and the associated proteasome may provide a new therapeutic approach for preventing Cd2+-induced cytotoxicity and renal injury.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Environmental Sciences
Caiying Zhang, Tianjin Lin, Gaohui Nie, Ruiming Hu, Shaoxing Pi, Zejing Wei, Chang Wang, Chenghong Xing, Guoliang Hu
Summary: The study revealed that the combination of Mo and Cd synergistically induces oxidative stress and triggers pyroptosis via the ROS/PTEN/PI3K/AKT axis in duck renal tubular epithelial cells.
ENVIRONMENTAL POLLUTION
(2021)
Article
Multidisciplinary Sciences
Soo Yeon Chae, Dowoon Nam, Do Young Hyeon, Areum Hong, Timothy Dain Lee, Sujin Kim, Dongjoon Im, Jiwon Hong, Chaewon Kang, Ji Won Lee, Daehee Hwang, Sang-Won Lee, Hugh Kim
Summary: The study investigated dose-dependent effects of topotecan on human neuroblastoma cells under different nutrient supply conditions, revealing increased survival rate with high concentration treatment. Network analysis indicated upregulation of DNA repair and cholesterol-mediated topotecan efflux by topotecan, leading to chemoresistance in neuroblastoma cells. This research provides a model for dose-dependent chemoresistance in neuroblastoma cells, offering potential for personalized chemotherapy screening strategies.
Article
Biochemistry & Molecular Biology
Lucia Cappabianca, Michela Sebastiano, Marianna Ruggieri, Maddalena Sbaffone, Veronica Zelli, Antonietta Rosella Farina, Andrew Reay Mackay
Summary: Patients with advanced neuroblastoma often develop resistance to the chemotherapy drug doxorubicin (Dox), leading to a poor prognosis. In this study, researchers identified a novel mechanism of Dox resistance involving the activation of an oncogenic variant called TrkAIII. They found that inhibiting this mechanism using certain kinase inhibitors could overcome Dox resistance. The study also provided insights into the molecular pathways and cellular processes involved in Dox-induced TrkAIII activation and resistance.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Endocrinology & Metabolism
Do-Hyung Lee, Kyung-Sun Heo, Chang-Seon Myung
Summary: This study investigated the mechanisms by which activation of orphan G protein-coupled receptor GPR41 enhances glucose uptake in C2C12 myotubes, using a GPR41-selective agonist. The results showed that AR420626 increased glucose uptake mediated by GPR41-dependent Ca2+ signaling, improving diabetes mellitus.
Article
Biotechnology & Applied Microbiology
Menghua Zhang, Taiki Sunaba, Yiting Sun, Takahiro Shibata, Kazunori Sasaki, Hiroko Isoda, Hideo Kigoshi, Masaki Kita
JOURNAL OF ANTIBIOTICS
(2020)
Article
Chemistry, Organic
Minako Hirukawa, Menghua Zhang, Lazaro M. Echenique-Diaz, Koji Mizota, Satoshi D. Ohdachi, Gerardo Begue-Quiala, Jorge L. Delgado-Labanino, Jorgelino Gamez-Diez, Jose Alvarez-Lemus, Leandro Galano Machado, Miguel Suarez Nunez, Takahiro Shibata, Hideo Kigoshi, Masaki Kita
TETRAHEDRON LETTERS
(2020)
Article
Chemistry, Multidisciplinary
Takayuki Ohyoshi, Hikaru Tano, Hideo Kigoshi
Summary: The synthesis of Aplysiasecosterol A involves pseudo-desymmetrizing acetalization and acyl radical cyclization to construct the unique tricyclic core. An efficient method for introducing the D ring fragment was developed using an organocuprate, advancing the total synthesis process.
BULLETIN OF THE CHEMICAL SOCIETY OF JAPAN
(2021)
Review
Chemistry, Multidisciplinary
Takayuki Ohyoshi, Hideo Kigoshi
Summary: This review discusses recent progress in the design of artificial analogs of Aplyronine A, including hybridization and structure-activity relationship studies, to further understand its novel mode of action as an antitumor marine macrolide inducing protein-protein interaction between actin and tubulin.
Article
Pharmacology & Pharmacy
Haruna Ebisu, Kana Shintani, Takumi Chinen, Yoko Nagumo, Shuya Shioda, Taisei Hatanaka, Akira Sakakura, Ichiro Hayakawa, Hideo Kigoshi, Takeo Usui
Summary: The study verified the antitumor activity of a specific γ-tubulin inhibitor, gatastatin, which showed weaker cytotoxicity compared to conventional microtubule inhibitors. Screening revealed that the Plk1 inhibitor, BI 2536, significantly increased the cytotoxicity of gatastatin, leading to cell cycle arrest at mitosis with abnormal spindles and mitotic cell death. Co-treatment with Plk1 and γ-tubulin inhibitors may offer a novel approach to cancer chemotherapy.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Multidisciplinary Sciences
Osamu Kutomi, Ryosuke Yamamoto, Keiko Hirose, Katsutoshi Mizuno, Yuuhei Nakagiri, Hiroshi Imai, Akira Noga, Jagan Mohan Obbineni, Noemi Zimmermann, Masako Nakajima, Daisuke Shibata, Misa Shibata, Kogiku Shiba, Masaki Kita, Hideo Kigoshi, Yui Tanaka, Yuya Yamasaki, Yuma Asahina, Chihong Song, Mami Nomura, Mamoru Nomura, Ayako Nakajima, Mia Nakachi, Lixy Yamada, Shiori Nakazawa, Hitoshi Sawada, Kazuyoshi Murata, Kaoru Mitsuoka, Takashi Ishikawa, Ken-ichi Wakabayashi, Takahide Kon, Kazuo Inaba
Summary: A novel subunit named DYBLUP has been discovered to modulate dynein activity in animal spermatozoa and a unicellular green alga through connecting the f/I1 motor domain and the tether complex. Chlamydomonas lacking the DYBLUP ortholog showed both positive and negative phototaxis, but became acclimated and attracted to high-intensity blue light, suggesting a mechanism to avoid toxic strong light via direct photoregulation of dyneins.
Article
Chemistry, Multidisciplinary
Tetsuya Inaba, Yoshimasa Ishizaki, Masayuki Igarashi, Masahito Yoshida, Hideo Kigoshi
Summary: The total synthesis of the piperazic acid-containing antibiotic cyclic peptide, hytramycin V, was achieved successfully. The study revealed a method to prevent unexpected cleavage of the peptide bond and explored the relationship between activity and chirality.
BULLETIN OF THE CHEMICAL SOCIETY OF JAPAN
(2021)
Article
Chemistry, Multidisciplinary
Tran Ngoc Linh, Takashi Arimura, Kenichi Tominaga, Hideo Kigoshi, Hiroko Isoda
Summary: New squalene receptors with open-chain polyether ligands were synthesized and found to interact with alkaline earth metal ions. Self-assembly behavior of the receptors at 25 degrees C was studied using fluorescence spectral analysis and microscopy, showing vesicle size changes induced by the metal ions. Cryogenic transmission electron microscopy confirmed the decrease in vesicle size of SQ-tetraEG upon addition of the metal ions.
SUPRAMOLECULAR CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Takayuki Ohyoshi, Yiwen Zhao, Kei Akemoto, Takuma Ishihara, Ayaka Taniguchi, Menghua Zhang, Hideo Kigoshi
Summary: The total synthesis of aplaminal with a unique skeleton was achieved, and the effects of different substituents at the para-position on cytotoxicity were investigated.
BULLETIN OF THE CHEMICAL SOCIETY OF JAPAN
(2022)
Article
Plant Sciences
Takayuki Ohyoshi, Yiwen Zhao, Hideo Kigoshi
Summary: Two new bromoditerpenes, azuriaplysins A (1) and B (2), were isolated from the sea hare Aplysia kurodai. Their relative stereostructures were determined by NMR analysis, and their absolute stereostructures were determined through total synthesis. Azuriaplysin B (2) and its enantiomer exhibited moderate cytotoxicity against HeLa S3 cells.
JOURNAL OF NATURAL PRODUCTS
(2022)
Article
Plant Sciences
Masahito Yoshida, Tomoya Matsushita, Shinji Kondo, Hiroko Isoda, Hideo Kigoshi
Summary: The structure of trichomide D has been revised through total synthesis, and the configuration of the chlorohydrin moiety was found to be a pivotal factor for exhibiting potent cytotoxicity against cancer cells.
JOURNAL OF NATURAL PRODUCTS
(2022)
Article
Chemistry, Organic
Keisuke Mitsugi, Toru Takabayashi, Takayuki Ohyoshi, Hideo Kigoshi
Summary: A strategy for constructing a tricyclo[4.3.1.0(3,7)]decane skeleton, common to many polycyclic polyprenylated acylphloroglucinols, has been established. The total synthesis of nemorosonol was achieved through a tandem Michael addition-intramolecular aldol reaction using 3-ethoxy-1-phenyl-2-proyn-1-one.
Correction
Plant Sciences
Takayuki Ohyoshi, Yiwen Zhao, Hideo Kigoshi
JOURNAL OF NATURAL PRODUCTS
(2022)
Article
Chemistry, Organic
Takayuki Ohyoshi, Atsuhiro Takano, Imari Kikuchi, Tomotaka Ogura, Mayu Namiki, Yuto Miyazaki, Takahiro Hirano, Shota Konishi, Yuta Ebihara, Koichi Takeno, Ichiro Hayakawa, Hideo Kigoshi
Summary: In this study, an aplyronine A-swinholide A hybrid was designed, synthesized, and evaluated for its biological activity. The methoxy group in the N,N,O-trimethylserine ester was found to be important for the compound's activity, and the side chain analog of swinholide A exhibited weaker actin-depolymerizing activity compared to that of aplyronine A.
ORGANIC & BIOMOLECULAR CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Didik Huswo Utomo, Akari Fujieda, Kentaro Tanaka, Momoko Takahashi, Kentaro Futaki, Kenta Tanabe, Hideo Kigoshi, Masaki Kita
Summary: Inspired by natural products based on protein-protein interactions (PPI), structurally-simplified analogs of aplyronine A were developed, with one analog showing potent actin-depolymerizing activity. These analogs are versatile actin-affinity tags that can accelerate research on the mode of action related to cytoskeletal dynamics and the development of PPI-based drug leads.
CHEMICAL COMMUNICATIONS
(2021)
