Article
Plant Sciences
Yalda Vasebi, Reza Khakvar, Boris A. Vinatzer
Summary: A total of 293 bacterial strains were isolated from the surface and internal tissues of almond and apricot trees in East Azerbaijan province, Iran. These strains belong to 15 different genera, with Pseudomonas, Pantoea, and Lysinibacillus being the most abundant. Some strains showed antagonistic activity against the causal agent of apricot canker disease.
JOURNAL OF PLANT PATHOLOGY
(2023)
Article
Microbiology
Tamara Rankovic, Ivan Nikolic, Tanja Beric, Tatjana Popovic, Jelena Lozo, Olja Medic, Slavisa Stankovic
Summary: This study analyzed the draft genome sequences of two pathogenic strains of Pseudomonas syringae pv. aptata to evaluate their survival strategies and pathogenic potential. The results showed differences in genome size, GC content, and protein-coding sequences between the two strains. Furthermore, differences in effector sequences were found to be related to the type III secretion system. This study provides important information on the genomic features, epidemiology, and pathogenic strategies of P. syringae pv. aptata.
MICROBIOLOGY SPECTRUM
(2023)
Article
Microbiology
Jun Liu, Xuejiang Zhang, Siyi Deng, Hua Wang, Youfu Zhao
Summary: This study investigates the importance of thiamine and its derivative TPP in the growth, stress resistance, and virulence of the plant pathogen PstDC3000. The results show that the absence of thiamine-related genes leads to reduced growth and virulence, but this can be restored with exogenous thiamine. Furthermore, a mutation in a lipoprotein-related gene affects stress tolerance and colonization. Overall, the study highlights the role of the thiamine biosynthetic pathway in the colonization and infection of PstDC3000.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Melanie R. Smee, Imperio Real-Ramirez, Catalina Zuluaga Arias, Tory A. Hendry
Summary: In this study, it was found that most aphid species are susceptible to infection and death when exposed to P. syringae, indicating that these bacteria are broadly useful for biological control. However, one aphid species was much more resistant to infection, suggesting that more testing on diverse aphid species is needed.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Plant Sciences
Jay Jayaraman, Minsoo Yoon, Lauren M. Hemara, Deborah Bohne, Jibran Tahir, Ronan K. Y. Chen, Cyril Brendolise, Erik H. A. Rikkerink, Matthew D. Templeton
Summary: Testing effector knockout strains of Pseudomonas syringae pv. actinidiae biovar 3 (Psa3) in their native kiwifruit host revealed nonredundant effectors contributing to Psa3 virulence, while complementation in weak kiwifruit pathogen P. syringae pv. actinidifoliorum (Pfm) identified redundant Psa3 effectors. Important Psa3 effectors include those that suppress pattern-triggered immunity (PTI) and early infection process effector redundancy targeting plant immunity hub RIN4.
Article
Biochemistry & Molecular Biology
Simone Luti, Sara Campigli, Francesco Ranaldi, Paolo Paoli, Luigia Pazzagli, Guido Marchi
Summary: Research on the bacterial canker disease caused by Pseudomonas syringae pv. actinidiae (Psa) biovar 3 has identified three potential genes coding for levansucrases in the Psa3 genome. Through cloning and expression in E. coli, lsc beta and lsc gamma were characterized for their different biochemical properties, indicating distinct roles in sucrose hydrolysis and levan synthesis, suggesting potential differential functions in the physiology of the bacterium.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Review
Biochemistry & Molecular Biology
Jiadai Huang, Chunyan Yao, Yue Sun, Quanjiang Ji, Xin Deng
Summary: This review summarizes the recent advances in the regulatory networks of transcription factors (TFs) involved in virulence-related pathways in Pseudomonas syringae. It also discusses future perspectives in terms of TF-mediated pathogenesis mechanisms and provides novel insights for combating P. syringae infections based on TF regulatory networks.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2022)
Article
Plant Sciences
Pei Qiao, Mei Zhao, Dehua Liu, Wei Guan, Qingrong Bai, Yuwen Yang, Tingchang Zhao
Summary: Watermelon diseases caused by pathogenic bacteria were endemic in Liaoning and Jilin Provinces from 2019 to 2020 in China. The pathogen, identified as Pseudomonas syringae, was reported for the first time in causing watermelon disease in China. The outbreak of bacterial leaf spot in watermelon was mainly caused by P. syringae clade 2a, indicating multiple sources of infection.
Article
Biochemistry & Molecular Biology
Yu Zhou, Shengxiong Huang, Wei Tang, Zhongqiu Wu, Siqi Sun, Yaqiong Qiu, Hongtao Wang, Xue Chen, Xiaofeng Tang, Fangming Xiao, Yongsheng Liu, Xiangli Niu
Summary: In this study, ten strains of Kiwifruit bacterial canker were isolated and sequenced, revealing divergences in pathogenicity and pathogenic-related genes among the strains. Pseudomonas syringae pv. actinidiae infection re-programmed the expression of host genes, with down-regulation of genes involved in plant-pathogen interactions, especially calcium signaling transduction and fatty acid elongation. These findings provide insights into understanding the molecular mechanism of Psa-kiwifruit interactions and improving resistance in the kiwifruit crop.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Microbiology
Stephany Angelia Tumewu, Hidenori Matsui, Mikihiro Yamamoto, Yoshiteru Noutoshi, Kazuhiro Toyoda, Yuki Ichinose
Summary: In this study, four dCache_1 type MCPs in Pta6605 were characterized, with PscA, PscB, and PscC2 responsible for sensing amino acids. Mutant strains of PscA, PscB, and PscC2 showed reduced chemotaxis to most amino acids, as well as effects on cellular functions and virulence in the host tobacco plant.
MICROBIOLOGICAL RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Isabel Orf, Hezi Tenenboim, Nooshin Omranian, Zoran Nikoloski, Alisdair R. Fernie, Jan Lisec, Yariv Brotman, Mariusz A. Bromke
Summary: This study analyzed the differences in resistance to the pathogen Pseudomonas syringae pv. tomato (Pst) between Arabidopsis thaliana accessions Col-0 and C24, and found that the resistance differences mainly involve salicylic-acid-dependent systemic acquired resistance mechanisms and differential activity of certain metabolic pathways.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Xiaobo Qin, Min Zhang, Qiaohong Li, Dalei Chen, Leiming Sun, Xiujuan Qi, Ke Cao, Jinbao Fang
Summary: By analyzing the transcriptomic profiles of kiwifruit shoots, it was found that different varieties have different defense mechanisms against Pseudomonas syringae pv. actinidiae (Psa). The PTI and cell walls play important roles in Maohua's defense against Psa infection, while the ETI is crucial in Hongyang's defense against the pathogen.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemical Research Methods
Leonardo Furci, David Pascual-Pardo, Jurriaan Ton
Summary: A non-destructive method using bioluminescent Pst::LUX was developed to quantify and visualize spatial-temporal colonisation by Pst in intact leaves of Arabidopsis and tomato. This method allowed for monitoring of Pst infection dynamics and detection of differences in colonisation between plant genotypes and treatments.
Article
Plant Sciences
Saray Santamaria-Hernando, Alvaro Lopez-Maroto, Clara Galvez-Roldan, Marti Munar-Palmer, Elizabet Monteagudo-Cascales, Jose-Juan Rodriguez-Herva, Tino Krell, Emilia Lopez-Solanilla
Summary: This study demonstrates that the perception of GABA and l-Pro by the PsPto-PscC chemoreceptor drives the entry of Pseudomonas syringae pv. tomato into the tomato apoplast. The recognition of these compounds leads to chemoattraction and is involved in the regulation of GABA catabolism. Mutation of the PsPto-PscC chemoreceptor reduces chemotactic response, impairing entry and reducing virulence in tomato plants. Interestingly, GABA and l-Pro levels increase upon pathogen infection and are involved in the regulation of the plant defense response.
MOLECULAR PLANT PATHOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Mingyang Zhang, Yilin Zhang, Xue Han, Juan Wang, Yu Yang, Biao Ren, Mian Xia, Gang Li, Rongxiang Fang, Hang He, Yantao Jia
Summary: Members of the Enterobacter genus, such as the newly isolated Enterobacter mori strain CX01, are gram-negative bacteria that can function as both plant growth-promoting bacteria and emerging pathogens. The CX01 strain lacks the typical type III secretion system found in pathogenic bacteria, but possesses other secretory systems along with various genes encoding potential virulence factors and systems for nutrient acquisition and phytohormone synthesis. Pathogenicity assays suggest that related strains may have similar host ranges, highlighting the importance of understanding the pathogenesis and ecology of such bacteria.
Article
Chemistry, Multidisciplinary
Doris A. van Bergeijk, Somayah S. Elsayed, Chao Du, Isabel Nunez Santiago, Anna M. Roseboom, Le Zhang, Victor J. Carrion, Herman P. Spaink, Gilles P. van Wezel
Summary: The biosynthetic gene clusters of Actinobacteria are poorly expressed in the laboratory. However, the stress hormone epinephrine has been found to promote their expression and influence the metabolism of Streptomyces. This response is mediated by the catechol moiety.
COMMUNICATIONS CHEMISTRY
(2022)
Article
Microbiology
M. Berlanga-Clavero, C. Molina-Santiago, A. M. Caraballo-Rodriguez, D. Petras, L. Diaz-Martinez, A. Perez-Garcia, A. de Vicente, V. J. Carrion, P. C. Dorrestein, D. Romero
Summary: This study reveals the effects of using Bacillus subtilis on melon seeds, showing changes in genetic and physiological responses that influence the metabolic and developmental status of the resulting plants. The extracellular matrix components of B. subtilis biofilms play a role in seed colonization and growth promotion. Understanding the mechanisms of bacterial growth promotion can lead to the development of customized growth-promoting strains.
NATURE MICROBIOLOGY
(2022)
Article
Microbiology
Carlos Molina-Santiago, John R. Pearson, Maria Victoria Berlanga-Clavero, Alicia Isabel Perez-Lorente, Antonio de Vicente, Diego Romero
Summary: Complex interactions between microbial populations can greatly influence the properties of microbial communities, which are highly relevant in diverse scientific areas. Utilizing modern microscopic techniques provides new insights into how bacteria interact at the cellular level, but our ability to study complex interactions in vivo remains somewhat limited.
MICROBIOLOGY SPECTRUM
(2022)
Article
Microbiology
Jesus M. Martinez-Cruz, Alvaro Polonio, Laura Ruiz-Jimenez, Alejandra Vielba-Fernandez, Jesus Hierrezuelo, Diego Romero, Antonio de Vicente, Dolores Fernandez-Ortuno, Alejandro Perez-Garcia
Summary: Phytopathogenic fungi have evolved mechanisms to manipulate plant defenses by preventing chitin recognition, with the CDA gene playing a crucial role in powdery mildew fungi. A new fungal chitin-binding effector, CHBE, has been identified as involved in the manipulation of chitin-triggered immunity in powdery mildew fungi.
Article
Biochemistry & Molecular Biology
Jose A. Gutierrez-Barranquero, Zaira Heredia-Ponce, Lorena Aguilera-Cobos, Adrian Pintado, M. Gonzalo Claros, Cayo Ramos, Francisco M. Cazorla, Antonio de Vicente
MOLECULAR PLANT-MICROBE INTERACTIONS
(2022)
Article
Microbiology
Maite Anorga, Miriam Urriza, Cayo Ramos, Jesus Murillo
Summary: This study revealed that Pseudomonas syringae carries multiple relaxase genes in its plasmids, which affect the transfer frequency and provide functional diversity and redundancy to the conjugation system, adding robustness to plasmid dissemination.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Plant Sciences
Rafael Villar-Moreno, Sandra Tienda, Jose A. Gutierrez-Barranquero, Victor J. Carrion, Antonio de Vicente, Francisco M. Cazorla, Eva Arrebola
Summary: This study investigated the interactions between rhizospheric Pc strains and plant roots, focusing on their effects on root performance. The researchers found that PCL1606 has a better adaptation to nutrient-rich environments and forms biofilms. Additionally, PCL1601 and PCL1606 strains were well adapted to avocado roots and showed biocontrol activity against a fungal pathogen. These findings suggest that these strains could be used to construct a beneficial microbial community associated with avocado plant roots.
FRONTIERS IN PLANT SCIENCE
(2022)
Review
Biochemistry & Molecular Biology
Isabel Padilla-Roji, Laura Ruiz-Jimenez, Nisrine Bakhat, Alejandra Vielba-Fernandez, Alejandro Perez-Garcia, Dolores Fernandez-Ortuno
Summary: RNA interference (RNAi) technology plays a crucial role in the study and management of powdery mildew and rust fungi by allowing gene function analysis and potential disease control approaches.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Ferdinand Marletaz, Elisa de la Calle-Mustienes, Rafael D. Acemel, Christina Paliou, Silvia Naranjo, Pedro Manuel Martinez-Garcia, Ildefonso Cases, Victoria A. Sleight, Christine Hirschberger, Marina Marcet-Houben, Dina Navon, Ali Andrescavage, Ksenia Skvortsova, Paul Edward Duckett, Alvaro Gonzalez-Rajal, Ozren Bogdanovic, Johan H. Gibcus, Liyan Yang, Lourdes Gallardo-Fuentes, Ismael Sospedra, Javier Lopez-Rios, Fabrice Darbellay, Axel Visel, Job Dekker, Neil Shubin, Toni Gabaldon, Tetsuya Nakamura, Juan J. Tena, Dario G. Lupianez, Daniel S. Rokhsar, Jose Luis Gomez-Skarmeta
Summary: Through the analysis of the little skate Leucoraja erinacea genome, it was found that the enlarged pectoral fins of skates are attributed to genomic rearrangements in the planar cell polarity pathway. Functional inhibition of planar cell polarity signaling resulted in a reduction in fin size, confirming its role in skate fin morphology.
Review
Biochemistry & Molecular Biology
Xinya Pan, Jos M. Raaijmakers, Victor J. Carrion
Summary: Bacteroidetes are widely found in soil ecosystems, and they interact with various eukaryotic hosts such as plants, animals, and humans. Their adaptability and genomic plasticity highlight their versatility in different environments. While much research has focused on clinically relevant Bacteroidetes, the study of plant-associated Bacteroidetes has been relatively understudied. To enhance our understanding of the functional roles of Bacteroidetes in plants and other hosts, this review explores their taxonomy, ecology, and their roles in nutrient cycling and host fitness. The review emphasizes their distribution, stress tolerance, genomic diversity, and importance in diverse ecosystems, including plant-associated microbiomes.
TRENDS IN MICROBIOLOGY
(2023)
Article
Plant Sciences
Adrian Pintado, Hilario Dominguez-Cervan, Victoria Pastor, Marissa Vincent, Soon Goo Lee, Victor Flors, Cayo Ramos
Summary: The iaaL alleles in P. savastanoi determine the production of IAA-Lys. iaaL(Psn) and iaaL(Psf) genes control the production of IAA-Lys in pv. savastanoi and pv. fraxini, respectively. Overexpression of these genes reduces the free IAA content and alleviates the inhibitory effect of IAA on plant root growth.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Biotechnology & Applied Microbiology
Jesus Camara-Almiron, Laura Dominguez-Garcia, Nadia El Mammeri, Alons Lends, Birgit Habenstein, Antonio de Vicente, Antoine Loquet, Diego Romero
Summary: This study demonstrates that recombinantly purified RcTasA retains the biochemical properties of the entire protein and shows that two aggregation-prone stretches and imperfect amino acid repeats in RcTasA contribute to its amyloid-like capacity. The study also identifies the critical role of residues D64, K68, and D69 in the structural function of TasA. Experiments with mutant versions of TasA reveal a partial loss of function in the assembly of the extracellular matrix or the stability of the core and amyloid-like properties. Overall, these findings provide insights into the polymerization process of TasA and the sequence determinants that govern the behavior of protein filaments in bacteria.
NPJ BIOFILMS AND MICROBIOMES
(2023)
Review
Microbiology
Nisrine Bakhat, Alejandra Vielba-Fernandez, Isabel Padilla-Roji, Jesus Martinez-Cruz, Alvaro Polonio, Dolores Fernandez-Ortuno, Alejandro Perez-Garcia
Summary: Fungal pathogens pose a growing threat to crop production worldwide, and chitin-induced immunity plays a crucial role in plant defense against these pathogens. However, fungi have developed various strategies to evade chitin-triggered immunity, including the secretion of effector proteins that modify or degrade chitin oligomers. This review provides an overview of chitin-triggered immune signaling and the mechanisms employed by fungi to suppress it, using powdery mildew fungi as an example.
Article
Biotechnology & Applied Microbiology
Eva Arrebola, Francesca R. Aprile, Claudia E. Calderon, Antonio de Vicente, Francisco M. Cazorla
Summary: Pseudomonas chlororaphis, a biocontrol rhizobacterium, produces Fit toxin and 2-hexyl-5-propyl resorcinol (HPR) as key compounds for effective biocontrol against fungal pathogens. Additional compounds such as hydrogen cyanide (HCN) and pyrrolnitrin (PRN) may play a supporting role in insecticidal activity.
INTERNATIONAL MICROBIOLOGY
(2022)