Article
Cell Biology
Kavita Narwani, Jeremy Stark, Daileen Cortez, Isaac Yang, Christian Au, Alissa Diaz, Catalina Guerra, Yutaka Niihara, Fawzia Bardag-Gorce
Summary: The study successfully produced rabbit cG-CAOMECS for reconstructing corneal epithelium in patients with bilateral limbal stem cell deficiency. Through cell culture and analysis methods, conclusions were drawn regarding cell growth and morphological changes, confirming the expression characteristics of basal cells and corneal epithelial-like cells in the produced cell sheet. The utilization of clinical-grade and FDA-approved reagents supported the successful production of cG-CAMECS.
CELL AND TISSUE RESEARCH
(2021)
Article
Cell & Tissue Engineering
Akihiro Fujino, Yasushi Fuchimoto, Yoshiyuki Baba, Nobutaka Isogawa, Takanori Iwata, Katsuhiro Arai, Makoto Abe, Nobuo Kanai, Ryo Takagi, Masanori Maeda, Akihiro Umezawa
Summary: This study investigated the effectiveness of autologous oral mucosa-derived cell sheets transplantation in preventing postoperative anastomotic stricture in patients with congenital esophageal atresia. The results showed that cell sheet transplantation may have a temporary and limited effect in preventing stricture. Future studies are needed to identify the most effective cases for transplantation and determine the appropriate timing of transplantation.
STEM CELL RESEARCH & THERAPY
(2022)
Review
Immunology
Zhaosong Meng, Tianle Yang, Dayong Liu
Summary: The oral mucosa is a protective barrier against pathogens, and inflammatory oral mucosal disorders are associated with type-2 epithelial-mesenchymal transition (EMT). This review comprehensively evaluates type-2 EMT in chronically inflammatory oral mucosal disorders, aiming to provide a foundation for future research and potential treatments.
FRONTIERS IN IMMUNOLOGY
(2022)
Review
Immunology
Dongjia Lin, Lisa Yang, Liling Wen, Huanzi Lu, Qianming Chen, Zhi Wang
Summary: Oral mucosal disease (OMD), also known as soft tissue oral disease, is a range of disorders affecting the mucosa and soft tissue in the oral cavity. The unclear etiology may involve dysbiosis of oral mucosa and saliva-resident microbiota, impacting immune response and epithelial barrier function, leading to OMD. Research suggests diverse oral microbial dysbiosis in different diseases, implying potential causal pathogens across various oral pathologies. This literature review explores how microbial dysbiosis interacts with mucosal adaptive immune cells and the epithelial barrier in OMD, focusing on representative OMDs including oral candidiasis, oral lichen planus, recurrent aphthous ulcer, oral leukoplakia, and oral squamous cell carcinoma.
MUCOSAL IMMUNOLOGY
(2021)
Article
Cell & Tissue Engineering
David Hui-Kang Ma, Yi-Jen Hsueh, Kevin Sheng-Kai Ma, Yueh-Ju Tsai, Shiang-Fu Huang, Hung-Chi Chen, Chi-Chin Sun, Ming-Tse Kuo, An-Shine Chao, Jui-Yang Lai
Summary: The study presented long-term outcomes of two clinical cases of COMET transplantation, demonstrating the promising potential of collagenase-based suspension culture technique for enhancing the long-term survival of transplanted OMECs.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Ophthalmology
Danni Gong, Chenxi Yan, Fei Yu, Dan Yan, Nianxuan Wu, Liangbo Chen, Siyi Zhang, Yao Fu
Summary: In this experiment, uncultured autologous rat oral mucosal epithelium (OME) was transplanted to alkali burned corneas in order to treat refractory persistent corneal epithelial defects (PED). The results showed that the transplantation accelerated the healing process and promoted the formation of the corneal epithelial barrier. In the further investigation, the transplanted oral epithelium cells were able to migrate to the corneal surface and transform into corneal epithelial phenotype. This suggests that oral epithelium may have a positive role in treating refractory PED.
EXPERIMENTAL EYE RESEARCH
(2022)
Article
Medicine, General & Internal
Wipawee Booranapong, Panida Kosrirukvongs, Sunisa Duangsa-ard, Kanda Kasetsinsombat, Khanit Sa-ngiamsuntorn, Adisak Wongkajornsilp
Summary: This study evaluated the efficacy and safety of using cultivated oral mucosal cells as autologous grafts for corneal replacement in patients with limbal stem cell deficiency. The results showed favorable outcomes, with patients who suffered chemical burns benefiting more clinically compared to those with SJS.
JOURNAL OF MEDICAL CASE REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Naiyou Liu, Charles M. Bowen, Mohammadali M. Shoja, Karen Larissa Castro de Pereira, Laxmi Priya Dongur, Antonio Saad, William K. Russell, Thomas Christopher Broderick, Jeffrey H. Fair, William Samuel Fagg
Summary: Myofibroblast activation is a cellular response to physiological or pathological insults. In this study, co-cultured amniotic cell conditioned media (coACCM) was found to activate myofibroblast, while amniotic fluid (AF) represses it. These findings suggest that different biologics can elicit different cellular responses and may have broad utility in the clinic and the lab.
Article
Ophthalmology
Pinnita Prabhasawat, Chareenun Chirapapaisan, Anuwat Jiravarnsirikul, Pattama Ekpo, Mongkol Uiprasertkul, Ranida Thamphithak, Sutthicha Matamnan, Chawikan Boonwong
Summary: This study aimed to analyze the phenotype of corneal epithelium in patients who underwent autologous cultivated oral mucosal epithelial transplantation (COMET) using in vivo confocal microscopy (IVCM) and impression cytology with immunofluorescence staining (ICIF). The results showed that successful cases demonstrated a cornea-like phenotype while failed cases mainly exhibited a conjunctival phenotype, with evidence of oral mucosal epithelial cells remaining in the cornea in both groups.
Article
Engineering, Biomedical
Suai Zhang, Ke Ye, Guanjie Gao, Xiaojing Song, Ping Xu, Jingrong Zeng, Bingbing Xie, Dandan Zheng, Liwen He, Jianping Ji, Xiufeng Zhong
Summary: In this study, we developed a simple and novel method to construct authentic iRPE sheets using human amniotic membrane as a natural scaffold. The decellularized amniotic membrane provided a bioscaffold similar to Bruch's membrane, supporting the growth and enhancing the characteristics and functions of iRPE cells. RNA-seq analysis revealed that the potential mechanism by which the decellularized amniotic membrane supported iRPE growth was the inhibition of epithelial-mesenchymal transition. Furthermore, the tissue-engineered iRPE sheets survived and maintained a monolayer when transplanted into the subretinal space of rabbits.
ACTA BIOMATERIALIA
(2022)
Article
Immunology
Maomao Zhao, Miaomiao Zhang, Kaiyuan Xu, Kaihui Wu, Ruiqi Xie, Ruowei Li, Qiong Wang, Weida Liu, Wenmei Wang, Xiang Wang
Summary: This study reveals that extracellular vesicles (EVs) derived from human oral mucosal epithelial cells (Leuk-1) have antifungal capabilities against Candida albicans. In vitro experiments showed that Leuk-1-EVs inhibited the growth and changed the morphology of C. albicans. In vivo experiments demonstrated that Leuk-1-EVs significantly alleviated tongue lesions, C. albicans infection, and oral mucosal inflammation in infected mice.
FRONTIERS IN IMMUNOLOGY
(2022)
Review
Transplantation
S. A. Borzenok, B. E. Malyugin, M. Yu Gerasimov, D. S. Ostrovsky
Summary: The article introduces a new treatment method for corneal blindness - cell therapy, which reconstructs the corneal epithelium of patients through autologous oral epithelial culture, improving vision and quality of life.
VESTNIK TRANSPLANTOLOGII I ISKUSSTVENNYH ORGANOV
(2021)
Article
Dentistry, Oral Surgery & Medicine
Iris Lin, Madhurima Datta, Denise M. Laronde, Miriam P. Rosin, Bertrand Chan
Summary: Oral cancer is a global health issue with high mortality due to late-stage diagnosis. Accessible oral cavity allows for screening, but some patients may lack follow-up services. Intraoral photos play a crucial role in timely identification and monitoring of oral lesions.
INTERNATIONAL DENTAL JOURNAL
(2021)
Article
Biology
Francesca Veronesi, Milena Fini, Lucia Martini, Paolo Berardinelli, Valentina Russo, Giuseppe Filardo, Berardo Di Matteo, Maurilio Marcacci, Elizaveta Kon
Summary: Early treatment of osteoarthritis (OA) is crucial for restoring daily and sport activities. This study compared the effects of three orthobiological treatments on OA and found that adipose-derived mesenchymal stromal cells (ADSCs) and amniotic epithelial stem cells (AECs) showed superior results in cartilage regeneration and inflammation reduction.
Review
Biochemistry & Molecular Biology
Hiroshi Toshida, Tomoto Kasahara, Masamichi Kiriyama, Yuma Iwasaki, Jobu Sugita, Kohei Ichikawa, Toshihiko Ohta, Katsumi Miyahara
Summary: The first product for ex vivo cultivated oral mucosal epithelial cell transplantation (COMET) was launched in Japan in June 2021. Two patients underwent COMET, and pathological and immunohistochemical examinations were conducted. The results showed that Ocural(R) can be accomplished without major complications, and stem cells derived from oral mucosa might be successfully engrafted.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)